Buchi FatExtractor E-500 User manual

FatExtractor E-500
Operation Manual
11593950 | A en

Imprint
Product Identification:
Operation Manual (Original) FatExtractor E-500
11593950
Publication date: 01.2019
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
E-Mail: [email protected]
BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi-
ence, especially with respect to structure, illustrations and technical details.
This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for com-
petitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.

BÜCHI Labortechnik AG Table of contents
Operation Manual FatExtractor E-500 iii
Table of contents
1 About this document...........................................................................................................7
1.1 Warning notices in this document........................................................................................................ 7
1.2 Symbols............................................................................................................................................... 7
1.2.1 Warning symbols .....................................................................................................................7
1.2.2 Mark-ups and symbols.............................................................................................................7
1.3 Trademarks.......................................................................................................................................... 8
2 Safety....................................................................................................................................9
2.1 Proper use ........................................................................................................................................... 9
2.2 Use other than intended ...................................................................................................................... 9
2.3 Staff qualification ................................................................................................................................. 9
2.4 Location of warning signs on the product .......................................................................................... 10
2.5 Protection devices ............................................................................................................................. 11
2.6 Residual risks .................................................................................................................................... 11
2.6.1 Hot surfaces...........................................................................................................................11
2.6.2 Empty beakers on heating plates........................................................................................... 11
2.6.3 Mechanical damages.............................................................................................................11
2.6.4 Glass breakage......................................................................................................................12
2.6.5 Glass damages during extraction process.............................................................................12
2.6.6 Overfilled extraction beakers .................................................................................................12
2.7 Personal protective equipment .......................................................................................................... 12
2.8 Modifications...................................................................................................................................... 12
3 Product description...........................................................................................................13
3.1 Description of function ....................................................................................................................... 13
3.1.1 Soxhlet Extraction..................................................................................................................13
3.1.2 Hot Extraction ........................................................................................................................14
3.1.3 Economic Continuous Extraction........................................................................................... 14
3.1.4 Description of the Analyte Protection (option)........................................................................ 14
3.2 Configuration ..................................................................................................................................... 16
3.2.1 Front view ..............................................................................................................................16
3.2.2 Rear view ...............................................................................................................................17
3.2.3 Upper connections on the rear side....................................................................................... 17
3.2.4 Lower connections on the rear side....................................................................................... 18
3.2.5 Installation board....................................................................................................................19
3.2.6 Interface ................................................................................................................................. 20
3.2.7 Pro Interface ..........................................................................................................................21
3.3 Type plate.......................................................................................................................................... 21
3.4 Scope of delivery ............................................................................................................................... 22
3.5 Technical data ................................................................................................................................... 22
3.5.1 FatExtractor E-500.................................................................................................................22
3.5.2 Ambient conditions.................................................................................................................23
3.5.3 Materials ................................................................................................................................23
4 Transport and storage ......................................................................................................24
4.1 Transport ........................................................................................................................................... 24
4.2 Storage .............................................................................................................................................. 24
4.3 Lifting the instrument ......................................................................................................................... 24

Table of contents BÜCHI Labortechnik AG
iv Operation Manual FatExtractor E-500
5 Installation..........................................................................................................................26
5.1 Before installation .............................................................................................................................. 26
5.2 Installation site................................................................................................................................... 26
5.3 Establishing electrical connections.................................................................................................... 26
5.4 Securing against earthquakes ........................................................................................................... 27
5.5 Connecting the coolant hose ............................................................................................................. 27
5.6 Connecting the solvent tubes to the condenser tank bottle............................................................... 28
5.7 Connecting the coolant tubes to the condenser tank bottle............................................................... 29
5.8 Preparing the tank bottle.................................................................................................................... 29
5.9 Preparing the instrument for Soxhlet Extraction ................................................................................ 30
5.9.1 Installations for Soxhlet Extraction.........................................................................................30
5.9.2 Preparing the extraction glass chamber Soxhlet ...................................................................32
5.9.3 Preparing the chamber for a Soxhlet Extraction ....................................................................32
5.10 Preparing the instrument for Hot Extraction....................................................................................... 34
5.10.1 Installations for Hot Extraction ...............................................................................................34
5.11 Preparing the instrument for Economic Continuous Extraction ......................................................... 35
5.11.1 Installations for Economic Continuous Extraction..................................................................35
5.11.2 Preparing the extraction glass chamber for an Economic Continuous Extraction .................36
5.11.3 Preparing the Economic Continuous Extraction chamber .....................................................36
5.12 Installing the protection shields.......................................................................................................... 37
5.12.1 Installing two protection shields (Soxhlet only) ......................................................................37
5.12.2 Installing one protection shield (Hot and Economic Continuous Extraction only).................. 38
5.13 Preparing the condenser ................................................................................................................... 39
5.14 Selecting a configuration ................................................................................................................... 41
5.15 Disassembling the condenser circuit ................................................................................................. 41
6 Operating the interface .....................................................................................................43
6.1 Layout of the interface ....................................................................................................................... 43
6.2 Function bar....................................................................................................................................... 43
6.3 Menu bar............................................................................................................................................ 44
6.3.1 Home menu ...........................................................................................................................45
6.3.2 Method menu.........................................................................................................................45
6.3.3 Configuration menu................................................................................................................45
6.3.4 Solvent menu.........................................................................................................................47
6.4 Status bar .......................................................................................................................................... 47
7 Operating the interface pro ..............................................................................................48
7.1 Layout of the interface ....................................................................................................................... 48
7.2 Function bar....................................................................................................................................... 49
7.3 Menu bar............................................................................................................................................ 49
7.3.1 Home menu ...........................................................................................................................50
7.3.2 Method menu.........................................................................................................................51
7.3.3 Configuration menu................................................................................................................51
7.3.4 Solvent menu.........................................................................................................................53
7.4 Status bar .......................................................................................................................................... 53

BÜCHI Labortechnik AG Table of contents
Operation Manual FatExtractor E-500 v
8 Editing a method ...............................................................................................................54
8.1 Creating a new method...................................................................................................................... 54
8.2 Changing the name of a method ....................................................................................................... 54
8.3 Setting the solvent of a method ......................................................................................................... 54
8.4 Setting the analyt protection .............................................................................................................. 55
8.5 Setting the extraction time of a method ............................................................................................. 55
8.6 Setting the extraction cycles of a method (Soxhlet Extraction only).................................................. 56
8.7 Setting the extraction heating level of a method................................................................................ 56
8.8 Setting the Soxhlet valve open time of a method (Soxhlet only) ....................................................... 56
8.9 Setting the rinse time of a method (Soxhlet and Hot Extraction only) ............................................... 57
8.10 Setting the rinse heating level of a method (Soxhlet and Hot Extraction only).................................. 57
8.11 Setting the number of drains for a method (Hot Extraction only)....................................................... 58
8.12 Setting Smart-Drying (Soxhlet and Economic Continuous Extraction only) ...................................... 58
8.13 Setting the drying time of a method................................................................................................... 59
8.14 Setting the drying heating level of a method...................................................................................... 59
9 Performing an extraction ..................................................................................................60
9.1 Adding solvent through the condenser .............................................................................................. 60
9.2 Putting the glass sample tube with frit in the extraction glass chamber ............................................ 60
9.3 Performing a Soxhlet Extraction ........................................................................................................ 61
9.3.1 Adjusting the optical sensor on the extraction glass chamber Soxhlet.................................. 61
9.3.2 Preparing the instrument for a Soxhlet Extraction .................................................................61
9.3.3 Selecting a method ................................................................................................................62
9.3.4 Selecting the extraction positions ..........................................................................................62
9.3.5 Starting a Soxhlet Extraction.................................................................................................. 62
9.3.6 Ending a Soxhlet Extraction...................................................................................................63
9.3.7 Shutting down the instrument ................................................................................................63
9.4 Performing a Hot Extraction............................................................................................................... 63
9.4.1 Preparing the instrument for a Hot Extraction........................................................................63
9.4.2 Selecting a method ................................................................................................................64
9.4.3 Selecting the extraction positions ..........................................................................................64
9.4.4 Starting a Hot Extraction........................................................................................................64
9.4.5 Ending a Hot Extraction .........................................................................................................65
9.4.6 Shutting down the instrument ................................................................................................65
9.5 Performing an Economic Continuous Extraction ............................................................................... 65
9.5.1 Preparing the instrument for an Economic Continuous Extraction ........................................65
9.5.2 Selecting a method ................................................................................................................66
9.5.3 Selecting the extraction positions ..........................................................................................66
9.5.4 Starting an Economic Continuous Extraction......................................................................... 66
9.5.5 Ending an Economic Continuous Extraction..........................................................................67
9.5.6 Shutting down the instrument ................................................................................................67
9.6 Exporting extraction parameters........................................................................................................ 67
9.6.1 Exporting extraction parameters without cloud connection.................................................... 67
9.6.2 Exporting extraction parameters with cloud connection......................................................... 68
10 Editing a solvent................................................................................................................69
10.1 Creating a new solvent ...................................................................................................................... 69
10.2 Changing the name of a solvent (own created solvents only) ........................................................... 69
11 Mobile connection .............................................................................................................70
11.1 Requirements for local network settings............................................................................................ 70
11.2 Preparing the instrument for app using.............................................................................................. 70
11.3 Connecting the instrument and app using a QR code....................................................................... 70
11.4 Connecting the instrument and app using a password...................................................................... 71

Table of contents BÜCHI Labortechnik AG
vi Operation Manual FatExtractor E-500
12 Cleaning and servicing .....................................................................................................72
12.1 Regular maintenance work................................................................................................................ 72
12.2 Removing the tank bottle................................................................................................................... 73
12.3 Draining solvent from the instrument................................................................................................. 74
13 Help with faults ..................................................................................................................75
13.1 Troubleshooting................................................................................................................................. 75
13.2 Draining solvent from Extraction glass chamber Soxhlet .................................................................. 76
13.3 Replacing the gliding elements.......................................................................................................... 76
14 Taking out of service and disposal..................................................................................78
14.1 Taking out of service.......................................................................................................................... 78
14.2 Disposal............................................................................................................................................. 78
14.3 Returning the instrument ................................................................................................................... 78
15 Appendix ............................................................................................................................79
15.1 Schematics ........................................................................................................................................ 79
15.1.1 Condenser circuit ...................................................................................................................79
15.1.2 Solvent collector schematic ...................................................................................................80
15.2 Spare parts and accessories ............................................................................................................. 80
15.2.1 Spare parts ............................................................................................................................80
15.2.2 Conversion kits ......................................................................................................................82
15.2.3 Accessories............................................................................................................................82
15.2.4 Consumables ......................................................................................................................... 83
15.2.5 Holder for extraction thimbles ................................................................................................83
15.2.6 Extraction thimbles................................................................................................................. 83

BÜCHI Labortechnik AG About this document | 1
Operation Manual FatExtractor E-500 7/84
1 About this document
This operation manual is applicable for all variants of the instrument.
Read this operation manual before operating the instrument and follow the instruc-
tions to ensure safe and trouble-free operation.
Keep this operation manual for later use and pass it on to any subsequent user or
owner.
BÜCHI Labortechnik AG accepts no liability for damage, faults and malfunctions re-
sulting from not following this operation manual.
If you have any questions after reading this operation manual:
uContact BÜCHI Labortechnik AG Customer Service.
https://www.buchi.com/contact
1.1 Warning notices in this document
Warning notices warn you of dangers that can occur when handling the device. There
are four danger levels, each identifiable by the signal word used.
Signal word Meaning
DANGER Indicates a danger with a high level of risk which could result in
death or serious injury if not prevented.
WARNING Indicates a danger with a medium level of risk which could result in
death or serious injury if not prevented.
CAUTION Indicates a danger with a low level of risk which could result in
minor or medium-severity injury if not prevented.
NOTICE Indicates a danger that could result in damage to property.
1.2 Symbols
The following symbols are displayed in this operation manual or on the device:
1.2.1 Warning symbols
Symbol Meaning
General warning
Hot surface
Breakable items
Hand injuries
1.2.2 Mark-ups and symbols
NOTE
This symbol draws attention to useful and important information.

1 | About this document BÜCHI Labortechnik AG
8/84 Operation Manual FatExtractor E-500
RThis character draws attention to a requirement that must be met before the in-
structions below are carried out.
uThis character indicates an instruction that must be carried out by the user.
ðThis character indicates the result of a correctly carried out instruction.
Mark-up Explanation
Window Software Windows are marked-up like this.
Tab Tabs are marked-up like this.
Dialog Dialogs are marked-up like this.
[ Button ] Buttons are marked-up like this.
[ Field names ] Field names are marked-up like this.
[ Menu / Menu item ] Menus or menu items are marked-up like this.
Status Status is marked-up like this.
Signal Signals are marked-up like this.
1.3 Trademarks
Product names and registered or unregistered trademarks that are used in this docu-
ment are used only for identification and remain the property of the owner in each
case.

BÜCHI Labortechnik AG Safety | 2
Operation Manual FatExtractor E-500 9/84
2 Safety
2.1 Proper use
The FatExtractor E-500 is designed for the extraction (solid-liquid extraction) of sam-
ples with known solvents and the reduction or drying of the extract.
The FatExtractor E-500 can be used in laboratories for the following tasks:
Soxhlet Extraction
Hot Extraction
Economic Continuous Extraction
The FatExtractor E-500 can be used with following solvents:
Chloroform
Diethyl ether
Hexane
Petroleum ether
Other solvents only with the prior written approval of BÜCHI Labortechnik AG.
2.2 Use other than intended
Use of any kind other than that described in Chapter2.1 "Proper use", page9 and
any application that does not comply with the technical specifications constitutes use
other than that intended.
In particular, the following applications are not permissible:
Use of the instrument in areas which require explosion-safe apparatus.
Processing samples which could be caused to explode or ignite by impact, friction,
heat or sparks (e.g. explosives).
Use of the instrument with samples that react with solvent.
Use of the instrument with solvents containing peroxides.
Use of the instrument with other than original BUCHI glassware.
Use of the instrument in overpressure situations.
Use of solvents without the prior written approval of BÜCHI Labortechnik AG.
2.3 Staff qualification
Unqualified persons are unable to identify risks and are therefore exposed to greater
dangers.
The device may only be operated by suitably qualified laboratory staff.
These operating instructions are aimed at the following target groups:
Users
Users are persons that meet the following criteria:
They have been instructed in the use of the device.
They are familiar with the contents of these operating instructions and the applica-
ble safety regulations and apply them.
They are able on the basis of their training or professional experience to assess
the risks associated with the use of the device.

2 | Safety BÜCHI Labortechnik AG
10/84 Operation Manual FatExtractor E-500
Operator
The operator (generally the laboratory manager) is responsible for the following as-
pects:
The device must be correctly installed, commissioned, operated and serviced.
Only suitably qualified staff may be assigned the task of performing the operations
described in these operating instructions.
The staff must comply with the local applicable requirements and regulations for
safe and hazard-conscious working practices.
Safety-related incidents that occur while using the device should be reported to the
manufacturer ([email protected]).
BUCHI service technicians
Service technicians authorized by BUCHI have attended special training courses and
are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair
measures.
2.4 Location of warning signs on the product
Hot surfaces Hand injuries

BÜCHI Labortechnik AG Safety | 2
Operation Manual FatExtractor E-500 11/84
2.5 Protection devices
1
2
Fig.1: Protection devices
1 Protection shield 2 Protection shield
2.6 Residual risks
The device has been developed and manufactured using the latest technological ad-
vances. Nevertheless, risks to persons, property or the environment can arise if the
device is used incorrectly.
Appropriate warnings in this manual serve to alert the user to these residual dangers.
2.6.1 Hot surfaces
The surfaces of the device can become very hot. If touched they can cause skin
burns.
uDo not touch hot surfaces or else wear suitable protective gloves.
2.6.2 Empty beakers on heating plates
Empty beakers on active heating plates lead to very hot surfaces and can damage
the instrument
uRemove empty beakers from the instrument.
uDeactivate heating positions not used.
2.6.3 Mechanical damages
If a device is damaged, sharp edges, moving parts or exposed electrical wires can
cause injuries.
uCheck device regularly for visible damage.
uMake sure that the locking mechanism of the protection shield is working properly.
uIf faults occur:
Switch off the device immediately.
Unplug the power cord.
Inform the operator.
Cool the device before taking any further action.
uDo not continue to use devices that are damaged.

2 | Safety BÜCHI Labortechnik AG
12/84 Operation Manual FatExtractor E-500
2.6.4 Glass breakage
Broken glass can cause severe cuts.
Not visible cracks on glassware can lead to breakage.
Minor damage to the ground joints impairs the sealing effect and lead to loss of sol-
vent during the extraction process.
uWear protective gloves when handling broken glassware.
uHandle the glass components carefully and do not drop them.
uAlways place the glassware in a suitable holder when they are not in use.
uPrevent any mechanical impact.
uPrevent thermal shocks on glassware.
uAlways visually inspect glass components for damage every time they are used.
uDo not continue to use glass components that are damaged.
2.6.5 Glass damages during extraction process
Glass damages during the extraction process can cause instrument damages caused
by solvents, coolant and fire.
uIf fault occurs:
Switch off the device immediately.
Unplug the power cord.
Inform the operator.
Let the temperature of the instrument decrease under observation until it is the
same as the ambient.
2.6.6 Overfilled extraction beakers
Overfilled extraction beakers can cause spills and lead to instrument damage or fire.
uDo not fill more than 170 mL in the extraction beakers.
uDo not fill more than 280 mL in the extraction beakers LSV.
2.7 Personal protective equipment
Depending on the application, hazards due to heat and/or corrosive chemicals may
arise.
uAlways wear appropriate personal protective equipment such as safety goggles,
protective clothing and gloves.
uMake sure that the personal protective equipment meets the requirements of the
safety data sheets for all chemicals used.
2.8 Modifications
Unauthorized modifications may impair safety and lead to accidents.
uUse only genuine BUCHI accessories, spare parts and consumables.
uTechnical modifications to the device or accessories should only be carried out
with the prior written approval of BÜCHI Labortechnik AG and only by authorized
BUCHI technicians.
BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized
modifications.

BÜCHI Labortechnik AG Product description | 3
Operation Manual FatExtractor E-500 13/84
3 Product description
3.1 Description of function
The FatExtractor E-500 is designed to carry out the following solid-liquid extraction
methods:
Soxhlet Extraction (see Chapter3.1.1 "Soxhlet Extraction", page13)
Hot Extraction (see Chapter3.1.2 "Hot Extraction", page14)
Economic Continuous Extraction (see Chapter3.1.3 "Economic Continuous Extrac-
tion", page14)
3.1.1 Soxhlet Extraction
Step 1 extraction
The sample is located in the extraction chamber.
The beaker contains the solvent.
The solvent is heated, vapor rises up to the condenser, con-
denses and drops into the extraction chamber with the sample.
The magnetic valve is closed, the solvent is collected up to the
optical sensor and extracts the analyte.
When the optical sensor is reached, the magnetic valve opens
and the solvent containing the analyte flows back into the beaker.
Step 2 rinsing
The solvent is heated, vapor rises up to the condenser, con-
denses and drops into the extraction chamber with the sample.
The magnetic valve is open, the solvent flows back into beaker,
the solvent is not collected.
This flushes traces of fat into the beaker.
Step 3 drying
The solvent is heated, vapor rises up to the condenser, con-
denses and flows into tank.
The analyte remains in the beaker.

3 | Product description BÜCHI Labortechnik AG
14/84 Operation Manual FatExtractor E-500
3.1.2 Hot Extraction
Step 1 extraction
The sample is located in the beaker.
The beaker contains the solvent.
The solvent is heated, vapor rises up to the condenser, con-
denses and drops into the beaker with the sample.
Step 2 rinsing
The solvent in the beaker is heated up and evaporated.
The vapor rises up to the condenser.
The condensed solvent flows into the beaker with the sample.
The tank bottle valve opens periodically and condensed solvent
flows in the tank bottle.
The solvent level reduce.
Step 3 drying
The solvent in the beaker is heated up and evaporated.
The vapor rises up to the condenser.
The tank bottle valve is open and condensed solvent flows in the
tank bottle.
The analyte remains in the beaker.
3.1.3 Economic Continuous Extraction
Step 1 extraction
The sample is located in the extraction chamber.
The beaker contains the solvent.
The solvent is heated, vapor rises up around the sample to the
condenser, condenses and drops back into extraction chamber
through the sample into the beaker.
Step 2 drying
The solvent is heated, vapor rises up around the sample to the
condenser, condenses and flows into tank.
The analyte remains in the beaker.
3.1.4 Description of the Analyte Protection (option)
The Analyte Protection protects the analyte from overheating.
NOTE
The presence of beaker and solvent is always detected wether the Analyte Protec-
tion is activated or deactivated.
The Analyte Protection triggers under the following situations:

BÜCHI Labortechnik AG Product description | 3
Operation Manual FatExtractor E-500 15/84
Soxhlet
Extraction
Hot
Extraction
Economic
Continuous
Extraction
Presence of beaker X X X
Presence of solvent
(Level check)
XXX
Extraction step X X X
Rinse step X X
(1. Interval)
X
Dry step X - X

3 | Product description BÜCHI Labortechnik AG
16/84 Operation Manual FatExtractor E-500
3.2 Configuration
3.2.1 Front view
4
5
11
10
12
3
6
7
8
9
Fig.2: Front view
1 Protection shield 2 Condenser rack
3 Chamber rack 4 Condenser solvent tank
5 Solvent tank 6 Beaker rack
7 Installation board 8 Analyte Protection sensor
(Option)
9 Heating plate 10 On/Off master switch
11 Interface
(Pro)

BÜCHI Labortechnik AG Product description | 3
Operation Manual FatExtractor E-500 17/84
3.2.2 Rear view
1
2
Fig.3: Rear view
1 Upper connections
See Chapter3.2.3 "Upper
connections on the rear side",
page17
2 Lower connections
See Chapter3.2.4 "Lower
connections on the rear side",
page18
3.2.3 Upper connections on the rear side
123
4
Fig.4: Connections on the top
1 Standard BUCHI communication port
(COM)
2 Standard BUCHI communication port
(COM)
3 LAN port 4 Port for the cooling water valve

3 | Product description BÜCHI Labortechnik AG
18/84 Operation Manual FatExtractor E-500
3.2.4 Lower connections on the rear side
1245
7
36
8
9
10
11
12
Fig.5: Connections on the buttom
1 Coolant water solvent tank in 2 Solvent in
3 Fuse 4 Power supply connection
5 Fuse 6 Inert gas connection
(option)
7 Coolant water out 8 Exhaust
9 Coolant water in 10 Type plate
11 Coolant water solvent tank out 12 Solvent out

BÜCHI Labortechnik AG Product description | 3
Operation Manual FatExtractor E-500 19/84
3.2.5 Installation board
4
5
6
7
13
12
11
10
1
2
3
8
9
Fig.6: Installation board
1 For UniversalExtractor E-800 2 Condenser rack Soxhlet Extraction
3 For UniversalExtractor E-800 4 Chamber rack Soxhlet Extraction
5 Condenser rack Economic
Continuous Extraction
6 Condenser rack Hot Extraction
7 Chamber rack Economic Continuous
Extraction
8 Beaker rack Hot Extraction
9 Beaker rack Soxhlet Extraction /
Economic Continuous Extraction
10 Solvent collector tube
11 Sensor plugs 12 Attachment for tube
13 Lift

3 | Product description BÜCHI Labortechnik AG
20/84 Operation Manual FatExtractor E-500
3.2.6 Interface
123
4
Fig.7: Interface
1Stop button 2 Screen
3 Function buttons 4 Navigation control