Buchi Pure C-810 User manual

Pure Chromatography Instruments
Operation Manual
11593990 | E en
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Imprint
Product Identification:
Operation Manual (Original) Pure Chromatography Instruments
11593990
Publication date: 05.2021
Version E
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
E-Mail: [email protected]
BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience,
especially with respect to structure, illustrations and technical details.
This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for
competitive purposes, nor made available to third parties. The manufacture of any component with the
aid of this manual without prior written agreement is also prohibited.
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Büchi Labortechnik AG Table of contents
Operation Manual Pure Chromatography Instruments iii
Table of contents
1 About this document...........................................................................................................8
1.1 Warning notices in this document..........................................................................................8
1.2 Symbols.................................................................................................................................8
1.2.1 Warning symbols ........................................................................................................8
1.2.2 Mark-ups and symbols................................................................................................8
1.3 Trademarks ...........................................................................................................................9
1.4 Connected devices ................................................................................................................9
2 Safety..................................................................................................................................10
2.1 Proper use ...........................................................................................................................10
2.2 Use other than intended ......................................................................................................10
2.3 Staff qualification .................................................................................................................10
2.4 Location of warning signs on the product ............................................................................11
2.5 Residual risks ......................................................................................................................11
2.5.1 Faults during operation .............................................................................................11
2.5.2 Malware infection due to connections with other devices or network .......................11
2.5.3 Damage to the internal memory due to incorrect shutting down of the instrument...12
2.6 Personal protective equipment ............................................................................................ 12
2.7 Modifications........................................................................................................................12
3 Product description...........................................................................................................13
3.1 Description of function .........................................................................................................13
3.2 Configuration .......................................................................................................................14
3.2.1 Front view Pure C-810 / C-815 .................................................................................14
3.2.2 Front view Pure C-830 / C-835 / C-850 ....................................................................15
3.2.3 Rear view..................................................................................................................15
3.2.4 Connections on gradient valve .................................................................................17
3.3 Type plate............................................................................................................................17
3.4 Scope of delivery .................................................................................................................18
3.5 Technical data .....................................................................................................................18
3.5.1 Pure Chromatography Instruments...........................................................................18
3.5.2 Ambient conditions ...................................................................................................20
3.5.3 Material .....................................................................................................................21
4 Transport and storage ......................................................................................................22
4.1 Transport .............................................................................................................................22
4.2 Storage ................................................................................................................................22
4.3 Lifting the instrument ...........................................................................................................23
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Table of contents Büchi Labortechnik AG
iv Operation Manual Pure Chromatography Instruments
5 Installation..........................................................................................................................24
5.1 Before installation ................................................................................................................24
5.2 Installation site.....................................................................................................................24
5.3 Securing against earthquakes ............................................................................................. 24
5.4 Establishing electrical connections......................................................................................25
5.5 Establishing solvent connection .......................................................................................... 25
5.6 Installing the solvent level sensor........................................................................................26
5.7 Assigning solvent to solvent lines........................................................................................26
5.8 Calibrating the solvent level sensor.....................................................................................27
5.9 Installing the exhaust (ELSD only) ...................................................................................... 28
5.10 Assembling the waste bottle................................................................................................28
5.11 Installing the waste level sensor..........................................................................................28
5.12 Calibrating the waste level sensor.......................................................................................29
5.13 Assembling the dry air supply (option) ................................................................................29
5.14 Assembling the gas connection (option)..............................................................................30
5.15 Assembling the solvent bottle platform (option)...................................................................31
5.16 Assembling the sample loop (Prep instruments only) ......................................................... 32
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Büchi Labortechnik AG Table of contents
Operation Manual Pure Chromatography Instruments v
6 Operation............................................................................................................................33
6.1 Control panel .......................................................................................................................33
6.1.1 Layout of control panel .............................................................................................33
6.1.2 Enter value................................................................................................................34
6.1.3 Menu bar...................................................................................................................35
6.1.4 Conditions panel .......................................................................................................37
6.1.5 Collection panel ........................................................................................................38
6.1.6 Gradient panel ..........................................................................................................39
6.1.7 Run panel .................................................................................................................40
6.1.8 Solvent selection panel.............................................................................................41
6.1.9 Detector selection panel ...........................................................................................41
6.1.10 Slope detection panel ...............................................................................................42
6.2 Editing a method..................................................................................................................42
6.2.1 Selecting a cartridge (Flash mode)...........................................................................42
6.2.2 Selecting a column (Prep mode) ..............................................................................44
6.2.3 Selecting a solvent....................................................................................................45
6.2.4 Editing the gradient...................................................................................................45
6.2.5 Editing detector selection..........................................................................................48
6.2.6 Editing the wavelength for the UV detector ..............................................................48
6.2.7 Editing the fraction collection criteria ........................................................................49
6.2.8 Editing the fraction collection time ............................................................................50
6.2.9 Editing the vapor sensor sensitivity ..........................................................................52
6.2.10 Saving a method.......................................................................................................52
6.3 Editing a solvent ..................................................................................................................53
6.3.1 Adding a new solvent................................................................................................53
6.3.2 Deleting a solvent .....................................................................................................54
6.4 Tasks during a separation ...................................................................................................54
6.4.1 Introducing a sample into the prep system ...............................................................54
6.4.2 Injecting a sample into the flash system ...................................................................55
6.4.3 Installing and removing a cartridge...........................................................................57
6.4.4 Installing and removing a column .............................................................................58
6.4.5 Inserting the fraction collection tray ..........................................................................60
6.4.6 Selecting an existing method....................................................................................61
6.4.7 Selecting values on the dialog box Sample Loading ................................................61
6.5 Performing a Flash separation using a method................................................................... 61
6.5.1 Preparing the instrument ..........................................................................................61
6.5.2 Selecting the flash mode (Pure C-850 only) .............................................................62
6.5.3 Starting Flash separation using a method ................................................................62
6.5.4 Changings during a separation.................................................................................62
6.5.5 Ending a Flash separation ........................................................................................63
6.5.6 Shutting down the instrument ...................................................................................63
6.6 Performing a Flash separation manually .............................................................................63
6.6.1 Preparing the instrument ..........................................................................................63
6.6.2 Selecting the flash mode (Pure C-850 only) .............................................................64
6.6.3 Starting Flash separation manually ..........................................................................64
6.6.4 Changings during a separation.................................................................................65
6.6.5 Ending a Flash separation ........................................................................................65
6.6.6 Shutting down the instrument ...................................................................................65
6.7 Performing a Prep separation using a method .................................................................... 66
6.7.1 Preparing the instrument ..........................................................................................66
6.7.2 Selecting the prep mode (Pure C-850 only) .............................................................66
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Table of contents Büchi Labortechnik AG
vi Operation Manual Pure Chromatography Instruments
6.7.3 Starting a Prep separation using a method ..............................................................66
6.7.4 Changings during a separation.................................................................................67
6.7.5 Ending a Prep separation .........................................................................................67
6.7.6 Shutting down the instrument ...................................................................................67
6.8 Performing a Prep separation manually .............................................................................. 67
6.8.1 Preparing the instrument ..........................................................................................68
6.8.2 Selecting the prep mode (Pure C-850 only) .............................................................68
6.8.3 Starting a separation.................................................................................................68
6.8.4 Changings during a separation.................................................................................69
6.8.5 Ending a Prep separation .........................................................................................69
6.8.6 Shutting down the instrument ...................................................................................69
6.9 Identifying fractions..............................................................................................................70
6.9.1 Identifying fractions by peak .....................................................................................70
6.9.2 Identifying fractions per vial ......................................................................................71
6.10 Importing and exporting data...............................................................................................71
6.10.1 Printing a run report ..................................................................................................71
6.10.2 Sending data to USB ................................................................................................72
6.10.3 Sending PDF to USB ................................................................................................72
7 Cleaning and servicing .....................................................................................................73
7.1 Regular maintenance work..................................................................................................73
7.2 Carrying out a data backup ................................................................................................. 74
7.3 Calibrating the display .........................................................................................................74
7.4 Cleaning the nebulizer.........................................................................................................75
7.5 Cleaning the check valve.....................................................................................................76
7.6 Cleaning the instrument.......................................................................................................77
7.7 Cleaning the solid loader .....................................................................................................78
7.8 Removing solvent from an used cartridge ........................................................................... 78
8 Help with faults ..................................................................................................................79
8.1 Faults, possible causes and solutions (general)..................................................................79
8.2 Faults, possible causes and solutions (cartridge)................................................................79
8.3 Faults, possible causes and solutions (solvent delivery)..................................................... 79
8.4 Faults, possible causes and solutions (sample injection).................................................... 81
8.5 Faults, possible causes and solutions (fraction collection).................................................. 81
8.6 Faults, possible causes and solutions (detection) ............................................................... 82
8.7 Error messages ...................................................................................................................83
8.8 Resetting the Fraction collector arm.................................................................................... 89
8.9 Replacing the shuttle valve rotor .........................................................................................90
9 Taking out of service and disposal..................................................................................92
9.1 Taking out of service ...........................................................................................................92
9.2 Disposal...............................................................................................................................92
9.3 Returning the instrument .....................................................................................................92
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Büchi Labortechnik AG Table of contents
Operation Manual Pure Chromatography Instruments vii
10 Appendix ............................................................................................................................93
10.1 Spare parts and accessories ...............................................................................................93
10.1.1 Accessories ..............................................................................................................93
10.1.2 Sample introduction accessories ..............................................................................94
10.1.3 Spare parts ...............................................................................................................95
10.1.4 Tubes........................................................................................................................95
10.1.5 Tools and adapter kits ..............................................................................................95
10.1.6 Wear parts ................................................................................................................96
10.2 Folder locations ...................................................................................................................96
10.3 Finding out separation conditions with the navigator...........................................................97
10.3.1 Open the navigator ...................................................................................................97
10.3.2 TLC Silica .................................................................................................................97
10.3.3 LC-C18 .....................................................................................................................98
10.3.4 LC-Transfer...............................................................................................................99
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1 | About this document Büchi Labortechnik AG
8/102 Operation Manual Pure Chromatography Instruments
1 About this document
This operation manual is applicable for all variants of the instrument.
Read this operation manual before operating the instrument and follow the
instructions to ensure safe and trouble-free operation.
Keep this operation manual for later use and pass it on to any subsequent user or
owner.
BÜCHI Labortechnik AG accepts no liability for damage, faults and malfunctions
resulting from not following this operation manual.
If you have any questions after reading this operation manual:
uContact BÜCHI Labortechnik AG Customer Service.
https://www.buchi.com/contact
1.1 Warning notices in this document
Warning notices warn you of dangers that can occur when handling the device.
There are four danger levels, each identifiable by the signal word used.
Signal word Meaning
DANGER Indicates a danger with a high level of risk which could result in
death or serious injury if not prevented.
WARNING Indicates a danger with a medium level of risk which could result in
death or serious injury if not prevented.
CAUTION Indicates a danger with a low level of risk which could result in mi-
nor or medium-severity injury if not prevented.
NOTICE Indicates a danger that could result in damage to property.
1.2 Symbols
The following symbols are displayed in this operation manual or on the device:
1.2.1 Warning symbols
Symbol Meaning
General warning
Dangerous electrical voltage
Flammable substances
Laser class 1
1.2.2 Mark-ups and symbols
NOTE
This symbol draws attention to useful and important information.
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Büchi Labortechnik AG About this document | 1
Operation Manual Pure Chromatography Instruments 9/102
RThis character draws attention to a requirement that must be met before the
instructions below are carried out.
uThis character indicates an instruction that must be carried out by the user.
ðThis character indicates the result of a correctly carried out instruction.
Mark-up Explanation
Window
Software Windows are marked-up like this.
Tab
Tabs are marked-up like this.
Dialog
Dialogs are marked-up like this.
[Button] Buttons are marked-up like this.
[Field names] Field names are marked-up like this.
[Menu / Menu item] Menus or menu items are marked-up like this.
Status Status is marked-up like this.
Signal Signals are marked-up like this.
1.3 Trademarks
Product names and registered or unregistered trademarks that are used in this
document are used only for identification and remain the property of the owner in
each case.
1.4 Connected devices
In addition to these operating instructions, follow the instructions and specifications
in the documentation for the connected devices.
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2 | Safety Büchi Labortechnik AG
10/102 Operation Manual Pure Chromatography Instruments
2 Safety
2.1 Proper use
The instrument is designed and built for laboratories.
The instrument can be used for the following tasks:
Purification
Separation of one or more compounds from a mixture
2.2 Use other than intended
Use of any kind other than that described in Chapter2.1 "Proper use", page10 and
any application that does not comply with the technical specifications (See
Chapter3.5 "Technical data", page18) constitutes use other than intended.
In particular, the following applications are not permissible:
Use of the instrument in areas which require explosion-safe instruments.
Use of the instrument with solvents containing peroxides.
Use the ELSD without connected exhaust.
Use the instrument for production purposes.
2.3 Staff qualification
Unqualified persons are unable to identify risks and are therefore exposed to greater
dangers.
The device may only be operated by suitably qualified laboratory staff.
These operating instructions are aimed at the following target groups:
Users
Users are persons that meet the following criteria:
They have been instructed in the use of the device.
They are familiar with the contents of these operating instructions and the
applicable safety regulations and apply them.
They are able on the basis of their training or professional experience to assess
the risks associated with the use of the device.
Operator
The operator (generally the laboratory manager) is responsible for the following
aspects:
The device must be correctly installed, commissioned, operated and serviced.
Only suitably qualified staff may be assigned the task of performing the
operations described in these operating instructions.
The staff must comply with the local applicable requirements and regulations for
safe and hazard-conscious working practices.
Safety-related incidents that occur while using the device should be reported to
the manufacturer ([email protected]).
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Büchi Labortechnik AG Safety | 2
Operation Manual Pure Chromatography Instruments 11/102
BUCHI service technicians
Service technicians authorized by BUCHI have attended special training courses and
are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair
measures.
2.4 Location of warning signs on the product
Fig.1: Location of the warning signs
Laser class 1
2.5 Residual risks
The device has been developed and manufactured using the latest technological
advances. Nevertheless, risks to persons, property or the environment can arise if
the device is used incorrectly.
Appropriate warnings in this manual serve to alert the user to these residual
dangers.
2.5.1 Faults during operation
If a device is damaged, sharp edges, moving parts or exposed electrical wires can
cause injuries.
uRegularly check device for visible damage.
uIf faults occur, switch off the device immediately, unplug the power cord and
inform the operator.
uDo not continue to use devices that are damaged.
2.5.2 Malware infection due to connections with other devices or network
Connections with other devices or a network can cause a malware infection to the
instrument.
uInstall antivirus software and firewall on the instrument before connecting it to
other devices or network.
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2 | Safety Büchi Labortechnik AG
12/102 Operation Manual Pure Chromatography Instruments
2.5.3 Damage to the internal memory due to incorrect shutting down of the
instrument
Incorrect shutting down of the instrument can cause damage to the internal
memory.
uShut down the instrument as described. See Chapter6 "Operation", page33
2.6 Personal protective equipment
Depending on the application, hazards due to heat and/or corrosive chemicals may
arise.
uAlways wear appropriate personal protective equipment such as safety goggles,
protective clothing and gloves.
uMake sure that the personal protective equipment meets the requirements of
the safety data sheets for all chemicals used.
2.7 Modifications
Unauthorized modifications can effect safety and lead to accidents.
uUse only genuine BUCHI accessories, spare parts and consumables.
uCarry out technical changes only with prior written approval from BUCHI.
uOnly allow changes to be made by BUCHI service technicians.
BUCHI accepts no liability for damage, faults and malfunctions resulting from
unauthorized modifications.
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Büchi Labortechnik AG Product description | 3
Operation Manual Pure Chromatography Instruments 13/102
3 Product description
3.1 Description of function
Pure instruments are purification devices designed to purify complex samples by
either flash chromatography, prep chromatography or by both.
Flash chromatography has the ability to separate gram size samples in short
period.
Prep-HPLC has the ability to separate complex samples at high resolution.
Pure instruments allow:
4 different solvents
Injection of liquid or solid sample
Separation on a cartridge or column
Identifying the compounds by UV and/or ELS detection
Collecting the desired fractions
Pure instrument schematic:
Gradient valve
UV
detector
Fraction
collection
Solvent 2
Solvent 3
Solvent 4
Solvent 1
Pump
Sample
introduction
Cartridge / column
ELSD
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3 | Product description Büchi Labortechnik AG
14/102 Operation Manual Pure Chromatography Instruments
3.2 Configuration
3.2.1 Front view Pure C-810 / C-815
5
10
9
8
123
4
6
7
Fig.2: Front view Pure C-810 / C-815
1 Control panel 2 RFID reader
3 On/Off switch 4 USB Port
5 Injection port 6 Cartridge holder
7 Flash pump 8 ELSD flow split valve
(C-815 only)
9 ELSD nebulizer
(C-815 only)
10 Fraction collection bay
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Büchi Labortechnik AG Product description | 3
Operation Manual Pure Chromatography Instruments 15/102
3.2.2 Front view Pure C-830 / C-835 / C-850
10
9
8
7
123
4
5
6
Fig.3: Front view Pure C-830 / C-835 / C-850
1 Control panel 2 RFID reader
(C-850 only)
3 On/Off switch 4 USB Port
5 Column holder 6 Prep HPLC pump
7 ELSD flow split valve
(C-835 / C-850 only)
8 ELSD nebulizer
(C-835 / C-850 only)
9 Prep sample injection valve 10 Fraction collection bay
3.2.3 Rear view
NOTE
All electrical connections are not limited energy.
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3 | Product description Büchi Labortechnik AG
16/102 Operation Manual Pure Chromatography Instruments
3
7
8
9
10
2
1
6
54
Fig.4: Rear view
1 Ventilation slot 2 Gradient valve
(See Chapter3.2.4 "Connections on
gradient valve", page17 )
3 Exhaust
(used for instruments with ELSD
only)
4 Signal connection
(for external air supply)
5 USB ports 6 Power supply connection
7 Fuse 8 On/Off master switch
9 Pressurized air inlet 10 LAN port
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Büchi Labortechnik AG Product description | 3
Operation Manual Pure Chromatography Instruments 17/102
3.2.4 Connections on gradient valve
6
5
4
3
21
10
987
Fig.5: Connections on the rear side
1 Waste line 2 Solvent line 4
3 Solvent line 3 4 Waste level sensor
5 Solvent line 2 6 Solvent line 1
7 Solvent level sensor line 1 8 Solvent level sensor line 2
9 Solvent level sensor line 3 10 Solvent level sensor line 4
3.3 Type plate
The type plate identifies the instrument. The type plate is located at the rear of the
instrument.
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3 | Product description Büchi Labortechnik AG
18/102 Operation Manual Pure Chromatography Instruments
Buchi Labortechnik AG
9230 Flawil / Switzerland
Type: xxx
SN: 10xxxxxxxx
Volt: xxx-xxx VAC
Frequ.: xx Hz
Power: xxxx W
Built: 20xx
Made in Switzerland
6
5
4
1
3
2
7
8
910 9
Fig.6: Type Plate
1 Company name and address 2 Instrument name
3 Serial number 4 Input voltage range
5 Frequency 6 Power consumption maximum
7 Year of manufacture 8 Product code
9 Approvals 10 Symbol for "Do not dispose of as
household waste"
3.4 Scope of delivery
NOTE
The scope of delivery depends of the configuration of the purchase order.
Accessories are delivered as per the purchase order, order confirmation, and
delivery note.
3.5 Technical data
3.5.1 Pure Chromatography Instruments
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Dimension
(W x D x H)
365 x 570 x
680 mm
365 x 570 x
680 mm
365 x 570 x
680 mm
365 x 570 x
680 mm
365 x 570 x
680 mm
Weight 25 kg 27 kg 31 kg 33 kg 33 kg
Power consumption 350 W 350 W 350 W 350 W 350 W
Connection voltage 100 - 240 V
± 10 %
100 - 240 V
± 10 %
100 - 240 V
± 10 %
100 - 240 V
± 10 %
100 - 240 V
± 10 %
Frequency 50 - 60 Hz 50 - 60 Hz 50 - 60 Hz 50 - 60 Hz 50 - 60 Hz
Fuse 4 A 4 A 4 A 4 A 4 A
Overvoltage cate-
gory
II II II II II
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Büchi Labortechnik AG Product description | 3
Operation Manual Pure Chromatography Instruments 19/102
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Pollution degree 2 2 2 2 2
IP Code IP 20 IP 20 IP 20 IP 20 IP 20
Solvents 4 4 4 4 4
Gas pressure
( maximum)
8 bar 8 bar 8 bar 8 bar 8 bar
Compressed air oil and dust
free
oil and dust
free
oil and dust
free
oil and dust
free
oil and dust
free
USB port 3 3 3 3 3
LAN port
(RJ45)
11111
RFID reader
(racks)
Yes Yes Yes Yes Yes
RFID reader
(cartridges)
Yes Yes No No Yes
Fraction collector
bay
closed closed closed closed closed
Lighted fraction col-
lector bay
Yes Yes Yes Yes Yes
Solvent level sensor Yes Yes Yes Yes Yes
Waste level sensor Yes Yes Yes Yes Yes
Vapor sensor Yes Yes Yes Yes Yes
Pump Flash Mode
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Gradient binary binary - - binary
Pressure range 0 - 50 bar 0 - 50 bar - - 0 - 50 bar
Flow rate 0 - 250 mL/
min
0 - 250 mL/
min
- - 0 - 250 mL/
min
Flow rate accuracy < 2 % < 2 % - - < 2 %
Functional principle self-priming self-priming - - self-priming
Specification 3 pistons
radial ar-
ranged
3 pistons
radial ar-
ranged
- - 2 pistons
parallel ar-
ranged
Pump Prep Mode
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Gradient - - binary binary binary
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3 | Product description Büchi Labortechnik AG
20/102 Operation Manual Pure Chromatography Instruments
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Pressure range - - 0-300 bar 0-300 bar 0-300 bar
Flow rate - - 0 - 100 ml/
min
0 - 100 ml/
min
0 - 100 ml/
min
Flow rate accuracy - - < 2 % < 2 % < 2 %
Functional principle - - self-priming self-priming self-priming
Specification - - 2 pistons
parallel ar-
ranged
2 pistons
parallel ar-
ranged
2 pistons
parallel ar-
ranged
UV Detector
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
UV - Vis wavelength
range
200 - 800
nm
200 - 800
nm
200 - 800
nm
200 - 800
nm
200 - 800
nm
Light sources Halogen /
Deuterium
Halogen /
Deuterium
Halogen /
Deuterium
Halogen /
Deuterium
Halogen /
Deuterium
Detector DAD DAD DAD DAD DAD
DAD scan entire
range
entire
range
entire
range
entire
range
entire
range
ELSD Detector
Pure
C-810
Pure
C-815
Pure
C-830
Pure
C-835
Pure
C-850
Light output laser - 0.3 mW - 1 mW 1 mW
Pressure carrier air - ~0.8 bar - ~0.8 bar ~0.8 bar
Pressure spray air - 3 - 3.5 bar - 3 - 3.5 bar 3 - 3.5 bar
Flow rate air - 2 - 2.5 L/
min
- 2 - 2.5 L/
min
2 - 2.5 L/
min
3.5.2 Ambient conditions
For indoor use only.
Max. altitude above sea level 2000 m
Ambient temperature 5‒40°C (25°C)
No maximum performance above 25°C
Maximum relative humidity 80% for temperatures up to 31°C
Storage temperature max. 45 °C
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