CAMAG ADC 2 User manual

INSTRUCTION MANUAL
ADC 2

ADC 2
Instruction Manual, November 2016 Page 1
Table of content
1. Introduction 2
1.1. CAMAG ADC2 2
1.2. Precautions 2
1.3. Parts supplied 5
1.4. Options 5
1.5. Spare parts 5
2. Installation 6
2.1. Instrument 6
2.2. Installation environment 7
2.3. Conditions for the installation 8
2.4. Mains connection 9
2.5. Connection to the PC 9
2.6. Mounting the exhaust tube 9
3. Getting started 11
3.1. Automatic instrument monitoring 11
3.2. Checking system functions 11
4. Manual operation 12
4.1. HPTLC plate / plate holder / foil holder 12
4.2. Drying module and plate lift 13
4.3. Positioning of Twin Trough Chamber 14
4.4. Developing and preconditioning solvent 15
4.5. Display and function of keys 16
5. Stand-Alone operation 18
5.1. Aborting an application in progress 19
5.2. Resetting the instrument 19
6. Display texts and diagrams 20
6.1. Texts and their meaning 20
6.2. The Normal operation mode dialog 21
6.3. The method stack dialog 22
6.4. The Dialog mode 23
6.5. The Parameters Input dialog 24
6.6. The Manual functions dialog 25
6.7. The Setup dialog 26
6.8. Error messages 27
7. Maintenance and trouble shooting 28
7.1. Cleaning 28
7.2. Decontamination 28
7.3. General maintenance 28
7.4. The Setup dialog 29
7.2.1. Set baud rate 29
7.2.2. Set display language 30
7.2.3. Set the LCD contrast 30
7.5. Replacing fuses 31
7.6. Maintenance Data Sheet 32
8. Technical data 32
Appendix A Option Humidity Control (022.8360) 33
1. General / saturated salt solution 33
2. Installation 34
3. Humidity control preparations 37
4. Preparation of molecular sieve 37
5. Humidity values in practice 38
6. Additional measurement values 39

ADC 2
Instruction Manual, November 2016 Page 2
1. Introduction
1.1. CAMAG ADC2
The Automatic Development Chamber (ADC2) permits reproduci-
ble chromatogram development without the need for constant
monitoring by the user. The ADC2 is shipped with a RS232 cable
and built-in interface for connecting to a computer with vision-
CATS/winCATS from which the instrument can be run but it can
be used in Stand-Alone mode too.
By means of ADC2, developing of HPTLC chromatograms will be
reproducible. The user is free from any process monitoring.
The user decides whether and how to precondition the plate, pre-
selects the distance to be migrated by the solvent system and the
drying parameters.
Development proceeds automatically and under controlled condi-
tions. A CCD-sensor monitors the progress of the solvent front;
the distance covered and the time elapsed are continuously dis-
played. On completion of development, the HPTLC plate is dried
with air and finally stays safely protected in the chamber until re-
moved by the user.
If used in Stand-Alone mode all method parameters are entered
via the keypad. Ten complete development programs can be
saved in nonvolatile memory and started at will without PC-
control.
HPTLC plates with a thickness of up to 2.5mm can be used in the
ADC2. If using aluminum foils it is mandatory to utilize a special
foil holder # 115.8363.
The ADC2 controlled by the software permits full control of the
development process by monitoring and reporting all parameters
including all TLC steps of an analysis, i.e. definition of plate mate-
rial, sample application, derivatization, development and evalua-
tion. All data are handled in a cGMP/cGLP compliant environment.
With the appropriate software option, the ADC2 can be used in a
21 CFR Part 11 compliant environment.
1.2. Precautions
•Please read this operating manual before starting the installa-
tion! This manual contains information and warnings the user
has to follow to ensure reliable operation of the instrument
•If the instrument is used in a manner not specified in this man-
ual, the protection provided by the equipment may be impaired

ADC 2
Instruction Manual, November 2016 Page 3
•Some interior parts of the instrument are under AC power.
Careless and improper use can cause injury. Unauthorized ma-
nipulations can cause damage
•This sign indicates (on instrument and in this manual) that fail-
ure to take note of the accompanying information may result
in damage of the instrument
•The instrument is manufactured and tested in accordance with
the respective European safety publications shown on the Dec-
laration of Conformity (DoC). The instrument complies with
safety class 1 and has been designed for indoor use only (IP
20). Further, this device has passed the CAMAG Quality Assur-
ance tests and has been delivered in safe operation condition.
For detailed instrument data see chapter technical data
•Attention: For safety reasons the instrument may only be used
for the purposes described in the operating manual
•To avoid injury use adequate safety equipment (protective
goggles, gloves etc. if applicable) when working with the in-
strument
•Before first operation, check whether the voltage shown on the
instrument matches your local mains voltage. The power cord
may only be connected to a grounded, fused (not higher than
16A) outlet. Do not use extension cords without ground con-
tact
•When working with the fluids of the instrument, be sure to
take the appropriate caution (protect your eyes from direct
contact with liquid)
•Risk of finger squeeze between moving parts; be careful clos-
ing the door
•The instrument may be used only by properly trained laboratory
staff
•The instrument may not be used in rooms with danger of ex-
plosions
•The instrument contains highly sophisticated electronics and
optical parts. It may be operated only in a non-condensing at-
mosphere in the temperature range outlined in the chapter
"Technical Data". Before installation and use, the instrument
should be acclimated properly
•Use a damp lint free cloth for cleaning the instrument surface.
Do not employ aggressive detergents
•Protect yourself and the instrument from electrostatic shock
which can cause damage to the electronic parts

ADC 2
Instruction Manual, November 2016 Page 4
•Only authorized personnel may open the instrument. Service
and repair is only to be performed by trained specialists. Use
spare parts and consumables supplied by CAMAG only. The
warranty is voided if parts from other sources are used. Check
the service manual before you start service to reduce product-
specific risks
•The power cord has to be removed before the instrument is
opened. It is not permitted to work on an instrument that has
been opened and is connected to the power supply
•Spare fuses must be of the type specified by the instrument
manufacturer. It is forbidden to short-circuit or manipulate fus-
es
•Use only the original, with the instrument delivered power cord
type
•If the instrument is found to be defective, it must be switched
off and steps must be taken to ensure that it cannot be
switched on by mistake
•If liquids penetrate the inside of the instrument, the power has
to be disconnected immediately. Small amounts of liquid can
be wiped off and/or dried by means of a hairdryer, with larger
amounts of liquid a service technician has to be called. A test
of functionality has to be performed in all cases
•Carry out all safety checks and the preventive maintenance as
recommended by the manufacturer in order to assure your per-
sonal safety and the full functionality of the instrument. Have
an authorized service specialist perform any service not de-
scribed by this manual
•See original manufacturers' manuals for further safety data on
third party equipment supplied with the system
•Lift/move/transport the system with the necessary care and
with sufficient manpower (install the transport security devices
if applicable, transport it only in the original packaging)
•The safety of any system incorporate with the equipment is the
responsibility of the assembler of the system
•This symbol indicates that this equipment must not be disposed
of as unsorted municipal waste but is to be collected separately
as electrical and electronic equipment (WEEE-Directive
2002/96/EC). To properly recycle the instrument or parts of it
you are requested to send the equipment back to the distribu-
tor, producer or an adequate collection system at the end of its
life. This will have potential effects on the environment and
human health

ADC 2
Instruction Manual, November 2016 Page 5
•The use of the instrument without adequate ventilation to out-
side air may constitute a health hazard depending on the sub-
stances in use
1.3. Parts supplied
Part no Description
140.8350
Tool kit, comprising:
672.0059
Exhaust tube
125.1021
RS232 connecting cable
362.0006
Fuses 1AT (2 pc)
705.0012
Allen wrench 3mm
960.0200
Cap for solvent inlet (2 pc)
700.1011
CCD-test plate ADC2/AMD2
115.8357
Plate holder
022.5261
Twin Trough Chamber
941.0006
Saturation pads (pack of 25)
Respective power cord
B.022.8350E
Instruction manual (English)
1.4. Options
Part no Description
022.8360
Humidity Control Module
022.8373
Foil holder
1.5. Spare parts
Part no Description
022 5261
Twin Trough chamber
022.8370
Saturation pads (pack of 100)
022.8372
Plate holder
022.8373
Foil holder
672.0059
Exhaust tube
960.0200
Cap for solvent inlet
022.8375
Filters for Humidity Control Module (pack of 10)
105.8352
Humidity control tank complete
956.0094
Humidity control tank (glass only)

ADC 2
Instruction Manual, November 2016 Page 6
2. Installation
2.1. Instrument
Observe the environmental requirements (2.2 Installation envi-
ronment) when setting up the instrument.
Carefully unpack all components and accessories listed on the
shipping list from the upper part of the packing. Make sure
the shipment is complete.
Remove the instrument carefully from the package and place it
on a table.
Attention: Do not pull out the instrument by holding it at the
blue top-cover, hold the instrument at the bottom when tak-
ing it out of the package and carrying it around!
Remove carefully the fixation under the Drying module.
For shipment, the Twin Trough Chamber has been securely
positioned below the bottom of the instrument.
Fig. 1: The ADC2 instrument

ADC 2
Instruction Manual, November 2016 Page 7
Fig. 2: The instrument ADC2 (opening the front door)
2.2. Installation environment
The place for installation must meet the following requirements:
Bench space Width 320mm
Depth 320mm without Humidity Option
Depth 460mm with Humidity Option
(additional for tubes 250mm)
Height 520mm
Weight 18.5kg
Operating
temperature
The temperature should be within a range of 18 to 30
degrees centigrade and free from significant variations.
Humidity Humidity and temperature conditions must not cause
condensation.
Atmospheric
conditions
Adequate ventilation free from acidic, alkaline or other
gas that may corrode metal or painted
surfaces must be
secured.

ADC 2
Instruction Manual, November 2016 Page 8
Further requirements:
•Do not place the instrument in a location where the tempera-
ture significantly changes (e.g. under an air conditioning duct
or by a window). Significant changes in temperature will affect
the performance of the unit
•Do not place the instrument in direct sunlight. Direct sunlight
will disturb the measurement of the CCD sensor
•Do not use the instrument in an environment with moving am-
bient air (draft)
•Do not expose the instrument to any strong vibration or shock
•Avoid placing the instrument near equipment that radiates
heat. Do not place the instrument near gas burners, electric
heaters or ovens
•Do not place the instrument near equipment that generates in-
tense magnetic fields such as electric welding equipment, high
frequency furnaces, pole transformers, etc.
•Protect the instrument from excessive dust
•Connect the instrument to power lines that are free from sud-
den changes or voltage fluctuations
•If you must use power motor driven equipment (such as a stir-
rer or shaker) in the same line as your instrument, ensure that a
noise reduction unit is in the same power line
2.3. Conditions for the installation
Confirm that the following requirements exist before installing the
instrument:
Power supply and ground
Line voltage: 100 – 240V (see rating plate on instrument).
Frequency: 50 / 60 Hz
Power capacity: 20 W
Ground terminal: A grounded outlet should be located within 2 me-
ters of the instrument.
Connection for Exhaust tube

ADC 2
Instruction Manual, November 2016 Page 9
2.4. Mains connection
Use the supplied power cord for connecting the instrument to the
mains outlet.
Fig. 3, Connections: 1 = RS232 Interface, 2 = Humidity module,3 = Power switch and
mains connection with fuse box.
2.5. Connection to the PC
The CAMAG ADC2 is shipped with a 9-pin RS 232 interface cable
for connection to the PC.
If you wish to use the instrument controlled by a software:
Connect the cable to the instrument and to a free COM-port
of the PC to enable data transfer.
2.6. Mounting the exhaust tube
The shipment contains an exhaust tube, which can be slipped on-
to the “black” air outlet fig.4, permitting exhaust solvent vapor to
be directed into a fume hood or other vent.
3
1
2

ADC 2
Instruction Manual, November 2016 Page 10
Fig. 4: Mounting the exhaust tube to the air outlet

ADC 2
Instruction Manual, November 2016 Page 11
3. Getting started
3.1. Automatic instrument monitoring
A sensor system controls and monitors the proper handling and
guarantees a monitored developing process. In case of failure a
signal will sound and an error message displayed. For error mes-
sages see chapter 6.8.
3.2. Checking system functions
Switch on the instrument. The ADC2 will now show the installed
firmware version and perform the following checks and tasks:
1. Initializing the drying module.
2. Initializing the lift drive.
If startup was successful the display will show:
* * ADC2 * *
SYSTEM READY
In this phase, only physical blocking of a drive can cause an error.
If necessary, check whether there are obstacles in the instrument.

ADC 2
Instruction Manual, November 2016 Page 12
4. Manual operation
4.1. HPTLC plate / plate holder / foil holder
The plate-holder is designed to attach and handle the HPTLC
plates in the ADC2. When inserting a plate into the plate holder
the layer side must be facing the user (‘LAYER’ side).
Fig. 5: Insert TLC plate in plate-holder
Important: While clamping tight the TLC plate the user
should be aware the plate fits the inside stop position and
the edges of holder and plate do not overlap.
Fig. 6: Plate clamped in Plate-holder
Important: When clamping the HPTLC plate make sure the
plate has hit the stop position (1) and the edges of the
holder (2) do not overlap the glass plate.
1
2

ADC 2
Instruction Manual, November 2016 Page 13
Note: Clamping foils works similar to that of HPTLC plates; the
two edges of the foil, left/right, must be inserted underneath the
respective down holders, fig.7.
Fig. 7: Mounting a foil in a foil-holder
4.2. Drying module and plate lift
To attach the plate or foil holder into the drying-module
make sure “LAYER” and substances are facing towards the
inside of the drying module, fig.8.
Fig. 8: Fixation of plate holder in drying module

ADC 2
Instruction Manual, November 2016 Page 14
4.3. Positioning of Twin Trough Chamber
To insert or take out the Twin Trough Chamber the lever (2) must
be in the upper position, see fig. 9.
When the Twin Trough Chamber has been positioned inside the
ADC2 make sure the chamber is touching the two stoppers at the
back, otherwise chromatography cannot be started. The
‘CAMAG’ label of the Chamber should normally face the user;
strongly inhomogeneous glass walls may thus require turning the
chamber around.
After a correct positioning of the Twin Trough Chamber, turn the
lever down in order to lower the drying module onto the edge of
the Chamber (1).
Fig. 9: 1 = Twin Trough Chamber, 2 = lever
When a saturation pad is needed, make sure it has been inserted
in the right position, i.e. the fold of the pad is contacting the bot-
1
2

ADC 2
Instruction Manual, November 2016 Page 15
tom of the Twin Trough Chamber while the shorter side is lying
on the bulge of the chamber, fig. 10.
Fig.10: Positioning of saturation pad
4.4. Developing and preconditioning solvent
The inlet reservoirs for developing (mobile phase) and precondi-
tioning solvents are located on top of the ADC2. The left inlet is

ADC 2
Instruction Manual, November 2016 Page 16
used for developing solvent and the right inlet for preconditioning
solvent. The volume of developing solvent should be 10ml, the
preconditioning volume 25ml.
Developing Preconditioning
solvent (10ml) solvent (25ml)
Fig.11: Inlet reservoirs
4.5. Display and function of keys
Control lamps
The control lamps in the display indicate the following status:
•POWER ON: this lamp is lit when the instrument is switched on.
•ON LINE: this lamp is lit when the instrument is ON-LINE with
PC and software.

ADC 2
Instruction Manual, November 2016 Page 17
The following keys are available:
DIALOG
Activates the manual parameter input mode (Method stack and
manual functions).
ARROW KEYS and 
Select the next / previous parameter (or method) in the dialog.
If the “SYSTEM READY” display is active, pressing these keys will
display the actual temperature and humidity. By pressing another
key, these measurements will be cleared.
ENTER
Confirms the current parameter, switches to the selected dialog or
(re-)starts the (last) selected method in manual function.
END
Interrupts the current application or switches back to the previous
dialog.
If the “SYSTEM READY” display is active, you can enter the man-
ual function stack.
Select any manual function by arrow keys or .
Press ENTER, to start a manual function or DIALOG, if you
want to change the manual parameters.
•IQ-TEST (performs instrument’s functionality tests)
•HUMIDITY CTRL (performs a timed humidity process)
•DRYING (performs a drying step)
•RINSING (performs a rinsing step)
•LAST RESULTS (displays the results of the last run)
RESET
Resets and re-initializes the instrument. The current method is in-
terrupted but all parameters remain in memory.
RUN
Shows the selection of stored methods.
Use the arrow keys or to select a method to start.
Press ENTER to start the selected method or DIALOG to
change the parameters of the actual method.

ADC 2
Instruction Manual, November 2016 Page 18
5. Stand-Alone operation
The instrument can be used in Stand-Alone mode. Up to 10
methods can be stored and used locally. Further you can start 4
permanent programmed methods (S1 to S4).
Note that locally started methods cannot be used in an analysis
created in visionCATS/winCATS,and that local parameter
changes are not documented in the software!
Procedure for Stand-Alone start:
Prepare the instrument for development.
Press the RUN key.
The most recently used method is displayed. With the or 
keys you can select another locally stored method.
Press the ENTER key to start the displayed method. The last
method is re-started if ENTER is pressed in the “SYSTEM
READY” mode.
Procedure for local input of parameters:
Press the RUN key.
The most recently used method is displayed. With the or 
key you can select another locally stored method.
Press the DIALOG key to locally change parameters of the se-
lected method.
Press the or key to select a parameter group (global pa-
rameters, parameters or save method) for local input and press
ENTER to confirm.
Now you can step through the parameters of the group by
means of the ENTER key or use the or key to change the
current parameter. To accelerate the change speed of a pa-
rameter you have to press the other arrow key a little bit later.
Press ENTER to confirm the new value and move on to the
next parameter.
Procedure for saving a changed method:
Select the parameter group Save method or press the END
key after having changed the parameters.
Select the memory position to save the new method by means
of the or key. Press ENTER to confirm position and save
the parameters.

ADC 2
Instruction Manual, November 2016 Page 19
Procedure for local delete of a saved method:
Press the RUN key.
The most recently used method is displayed. With the or 
key you can select the locally stored method to delete.
Press the key combination RUN-END (press and hold RUN,
then press END) and confirm deleting with ENTER.
Flow diagrams for these dialogs are depicted in chapter 6.
5.1. Aborting an application in progress
Procedure:
Press the END key
The development in progress is aborted and if the plate was
immersed in solvent, a drying process will follow. An aborted
development cannot be continued.
5.2. Resetting the instrument
Procedure:
Press the RESET key
This will abort all activities in progress and re-initialize the in-
strument.