CAMAG LINOMAT 5 User manual

INSTRUCTION MANUAL
LINOMAT 5

Linomat 5
Instruction manual, March 2016 Page 1
Table of content
1Introduction 2
1.1 CAMAG Linomat 5 2
1.2 Precautions 2
1.3 Parts supplied 4
1.4 Spare parts 4
2Installation 5
2.1 Instrument 5
2.2 Installation environment 6
2.3 Conditions for the installation 7
2.4 Gas supply 7
2.5 Mains connection 7
2.6 Connection to the PC 8
3Getting started 8
3.1 Automatic instrument monitoring 8
3.2 Checking system functions 8
3.3 Inserting a dosage syringe 9
3.4 Setting the application position 10
3.5 Loading a TLC plate or foil 10
3.6 Waste plate 11
4Manual operation 12
4.1 Display and function of keys 12
4.2 Stand-alone operation 13
4.3 Aborting an application in progress 14
4.4 Resetting the instrument 14
4.5 Docking the syringe 14
5Maintenance and trouble shooting 15
5.1 General maintenance 15
5.2 The Setup dialog 15
Set baud rate 15
Set display language 15
Set the LCD contrast 16
5.3 Handling of the HAMILTON-syringe 16
5.4 Adjusting the spray gas 16
5.5 Cleaning the jet assembly 17
5.6 Replacing fuses 17
5.7 Maintenance Data Sheet 18
6Technical data 18
7Display texts and diagrams 19
7.1 Normal mode 19
7.2 The System ready dialog 20
7.3 The syringe selection 20
7.4 The method stack dialog 21
7.5 The parameter type selection dialog 22
7.6 The parameters input dialog 23
7.7 The Setup dialog 24
7.8 Error messages 25
Declaration of Conformity (DoC)

Linomat 5
Instruction manual, March 2016 Page 2
1Introduction
1.1 CAMAG Linomat 5
The CAMAG Linomat 5 is a semiautomatic sample application de-
vice for use in qualitative, quantitative and preparative Thin-layer
chromatography. The Linomat 5 is shipped with EquiLink, RS232
cable and built in interface for connecting to a computer from
which the instrument can be programmed.
The parameters for up to 10 application programs can be stored
for stand-alone operation without PC- control.
The Linomat 5 is software controlled, visionCATS/winCATS con-
trols and reports all parameters and steps of the TLC analysis in-
cluding definition of plate material, sample application, derivatiza-
tion, development and evaluation. All data are handled in a
cGMP/cGLP-compliant manner.
1.2 Precautions
•Please read this operating manual before starting the installa-
tion! This manual contains information and warnings the user
has to follow to ensure reliable operation of the instrument.
•Some interior parts of the instrument are under AC power.
Careless and improper use can cause injury. Unauthorized ma-
nipulations can cause damage
•This sign indicates (on instrument and in this manual) that fail-
ure to take note of the accompanying information may result
in damage of the instrument
•The instrument is manufactured and tested in accordance with
the respective European safety publications shown on the Dec-
laration of Conformity (DoC). The instrument complies with
safety class 1 and has been designed for indoor use only (IP
20). Further, this device has passed the CAMAG Quality Assur-
ance tests and has been delivered in safe operation condition.
For detailed instrument data see chapter technical data
•Attention: For safety reasons the instrument may only be used
for the purposes described in the operating manual
•To avoid injury use adequate safety equipment (protective
goggles, gloves etc. if applicable) when working with the in-
strument
•Before first operation, check whether the voltage shown on the
instrument matches your local mains voltage. The power cord
may only be connected to a grounded, fused (not higher than

Linomat 5
Instruction manual, March 2016 Page 3
16A) outlet. Do not use extension cords without ground con-
tact
•When working with the fluids of the instrument, be sure to
take the appropriate caution (protect your eyes from direct
contact with liquid)
•The instrument may be used only by properly trained laboratory
staff
•The instrument may not be used in rooms with danger of ex-
plosions
•The instrument contains highly sophisticated electronics and
optical parts. It may be operated only in a non-condensing at-
mosphere in the temperature range outlined in the chapter
"Technical Data". Before installation and use, the instrument
should be acclimated properly
•Use a damp lint free cloth for cleaning the instrument surface.
Do not employ aggressive detergents
•Protect yourself and the instrument from electrostatic shock
which can cause damage to the electronic parts
•Only authorized personnel may open the instrument. Service
and repair is only to be performed by trained specialists. Use
spare parts and consumables supplied by CAMAG only. The
warranty is voided if parts from other sources are used. Check
the service manual before you start service to reduce product-
specific risks
•The power cord has to be removed before the instrument is
opened. It is not permitted to work on an instrument that has
been opened and is connected to the power supply
•Spare fuses must be of the type specified by the instrument
manufacturer. It is forbidden to short-circuit or manipulate fus-
es
•Use only the original, with the instrument delivered power cord
type
•If the instrument is found to be defective, it must be switched
off and steps must be taken to ensure that it cannot be
switched on by mistake
•If liquids penetrate the inside of the instrument, the power has
to be disconnected immediately. Small amounts of liquid can
be wiped off and/or dried by means of a hairdryer, with larger
amounts of liquid a service technician has to be called. A test
of functionality has to be performed in all cases
•Carry out all safety checks and the preventive maintenance as
recommended by the manufacturer in order to assure your per-
sonal safety and the full functionality of the instrument. Have

Linomat 5
Instruction manual, March 2016 Page 4
an authorized service specialist perform any service not de-
scribed by this manual
•See original manufacturers' manuals for further safety data on
third party equipment supplied with the system
•Lift/move/transport the system with the necessary care and
with sufficient manpower (install the transport security devices
if applicable, transport it only in the original packaging)
•The safety of any system incorporate with the equipment is the
responsibility of the assembler of the system
•This symbol indicates that this equipment must not be disposed
of as unsorted municipal waste but is to be collected separately
as electrical and electronic equipment (WEEE-Directive
2002/96/EC). To properly recycle the instrument or parts of it
you are requested to send the equipment back to the distribu-
tor, producer or an adequate collection system at the end of its
life. This will have potential effects on the environment and
human health
1.3 Parts supplied
Part no Units Description
695.0014
1
Sample Syringe 100µL
140.7808-1
1
Tool kit, comprising:
715.2329
1
Down holder for TLC sheets
715.2328
1
Down holder for TLC plates
140.0450-1
1
Pneumatics connecting kit
125.1021
1
RS232 connecting cable
362.0006
2
Fuses 1AT
705.0018
1
Allen wrench 2.5mm
1
Respective power cord
B.022.7808E
1
Instruction manual (English)
1.4 Spare parts
Part no
Description
695.0014
Sample Syringe 100µL
695.0015
Sample Syringe 500µL
022.7830
Plunger for syringe 100µL
022.7831
Plunger for syringe 500µL
115.0570
Venturi jet assembly

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Instruction manual, March 2016 Page 5
2Installation
2.1 Instrument
Observe the environmental requirements (2.2 Installation envi-
ronment) when setting up the instrument.
Carefully unpack all components and accessories listed on the
shipping list from of the upper part of the packing. Make sure
the shipment is complete.
The plate support has been secured with foam pieces for ship-
ment. To unlock the plate support, remove all foam pieces from
the slit in the instrument base.
Fig. 1: The instrument
Remove the dosage turret carefully from the package and
place it on the turret support (fig. 2).
Make sure that the dosage turret has been properly positioned
on the fixation (3) before tightening the two turret screws (2
and 4) by means of the screwdriver supplied.
Tighten screw (1) by hand.
Connect the turret cable with connector (2) as shown in fig. 3.

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Instruction manual, March 2016 Page 6
Fig. 2: Mounting the dosage turret
2.2 Installation environment
The place for installation should meet the following requirements:
Bench space Width 360mm
Depth 510mm (add space for cables approx. 100mm)
Height 410mm
Weight 12.5kg

Add adequate space for a PC next to the instrument
Operating
temperature
The temperature should be within a range of 18 to 35
degrees centigrade and free from significant variations.
Humidity Humidity and temperature conditions must not cause
condensation.
Atmospheric
conditions
Adequate ventilation free from acidic, alkaline or other
gas that may corrode metal or painted surfaces must
be secured.
Further requirements:
•Do not place the instrument in a location where the tempera-
ture significantly changes (e.g. under an air conditioning duct
or by a window). Significant changes in temperature will affect
the performance of the unit.
•Do not place the instrument in direct sunlight. Direct sunlight
may create significant temperature changes which will affect
the performance of the system.
•Do not use the instrument in an environment with moving am-
bient air (draft).
•Do not expose the instrument to any strong vibration or shock.
•Avoid placing the instrument near equipment that radiates
heat. Do not place the instrument near gas burners, electric
heaters or ovens.

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Instruction manual, March 2016 Page 7
•Do not place the instrument near equipment that generates in-
tense magnetic fields such as electric welding equipment, high
frequency furnaces, pole transformers, etc.
•Protect the instrument from excessive dust.
•Connect the instrument to power lines that are free from sud-
den changes or voltage fluctuations.
•If you must use power motor driven equipment (such as a stir-
rer or shaker) in the same line as your instrument, ensure that a
noise reduction unit is in the same power line
2.3 Conditions for the installation
Confirm that the following requirements exist before installing the
instrument:
Power supply and ground
Line voltage: 100 – 240V (see rating plate on instrument).
Frequency: 50 / 60 Hz
Power capacity: 20 W
Ground
terminal:
A grounded outlet should be within 2 meters of
the instrument.
Gas supply
N2 or
compressed air
Required pressure 4 - 6 bar (58 -87 psi)
Gas consumption 1 L/min
2.4 Gas supply
Connect the supplied pressure tubing to the gas inlet nipple
(fig. 3, item 4) on the backside of the instrument.
Connect the other end of the tubing to the gas supply.
If the outlet of your gas supply does not fit, the N2/compressed air
connection kit allows connection to standard ¼” tubing.
Adjust the primary pressure to 4 - 6 bar (58-87 psi).
The message:„WAITING FOR SYSTEM PRESSURE“ will be displayed
if the instrument is started with too low pressure.
If long thin tubing is used it is recommended to increase the pri-
mary pressure to 6-8 bar (87-115 psi).
2.5 Mains connection
Ensure that the voltage shown on the rating plate (left side)
matches that of the mains.
Use the supplied power cord for connecting the instrument to
the mains outlet.

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Instruction manual, March 2016 Page 8
Fig. 3: Connections. 1 = RS232 Interface, 2 = dosage turret connection, 3 = Power
switch and mains connection with fuse box, 4 = gas connection
2.6 Connection to the PC
The CAMAG Linomat 5 is shipped with a 9-pin interface cable for
connection to the PC.
Connect the cable to the instrument and to a free COM-port
of the PC to enable data transfer.
3Getting started
3.1 Automatic instrument monitoring
A sensor in the CAMAG Linomat 5 monitors the pressure of the
gas supply and generates an error message if the pressure drops
below 3.5 bar during spray-on application.
All valves are controlled in such way that they close if the power
fails or the instrument is switched off.
3.2 Checking system functions
Switch on the instrument.
The Linomat 5 will now perform the following checks and tasks:
1. Initializing the plate stage drive
2. Initializing the syringe drive.
If startup was successful the display will read:
** Linomat 5 **
Instrument ready
In this phase, only physical blocking of a drive can cause an error.
If necessary, check if all transport locks have been removed and
whether there are foreign objects in the instrument. (e.g. below
the plate stage).

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Instruction manual, March 2016 Page 9
3.3 Inserting a dosage syringe
Installation of a syringe (see fig. 4):
Make sure plate stage is in front position and the syringe lever
arm is at the top of the turret.
Fill the syringe with at least the minimum amount indicated on
the display.
Use the left hand to move the lever arm (1) to the left and
hold it there.
Carefully insert the syringe through the syringe holder (2) into
the spray head (3) and lower the syringe carefully until it rests
in the spray head. This way the needle is guided through the
spray nozzle.
The volume of the syringe in use must be set in the user dialog. If
a wrong syringe volume is entered, the application volumes will all
be wrong.
Fig. 4: Installation of the syringe. 1= Lever arm, 2 = syringe holder, 3 = Spray head as-
sembly with nozzle, 4 = slide coupler, 5 = scale pointer
Entering syringe volume:
Press the DIALOG key on the instrument to start the user dia-
log.
Use the arrow keys and to select the volume of the sy-
ringe in use.
Confirm by pressing the ENTER key on the instrument.