CAMAG DBS-MS 500 User manual

INSTRUCTION MANUAL
DBS-MS 500

Content
1Introduction.................................................................................................................................... 3
1.1 Intended use of the equipment ................................................................................................. 3
1.2 Basic overview........................................................................................................................... 3
1.3 Fluidic principle ......................................................................................................................... 5
1.4 Precautions ............................................................................................................................... 6
1.5 Parts supplied............................................................................................................................ 9
1.5.1 Optional Parts ....................................................................................................................... 9
2Unpacking/Installation ................................................................................................................ 10
2.1 Unpacking .............................................................................................................................. 10
2.2 Installation environment.......................................................................................................... 10
2.3 Removal of shipping protection............................................................................................... 11
2.4 Installation of the instrument .................................................................................................. 13
Fluidics............................................................................................................................................ 13
Electrics........................................................................................................................................... 15
2.5 Priming ................................................................................................................................... 16
3Getting started ............................................................................................................................. 18
3.1 The HMI (Human-Machine interface) ...................................................................................... 18
3.2 Setting up an extraction with HMI support.............................................................................. 19
General method settings................................................................................................................. 20
Sample settings............................................................................................................................... 20
Image processing settings ............................................................................................................... 24
Internal Standard settings ............................................................................................................... 25
Extraction settings........................................................................................................................... 26
Start the method ............................................................................................................................ 27
3.3 Setting up a stack extraction method ...................................................................................... 28
3.4 Additional screens................................................................................................................... 29
3.5 Setting up an extraction with software (Chronos for CAMAG) support ................................... 29
4Maintenance and Service ............................................................................................................ 30
4.1 Cleaning ................................................................................................................................. 30
4.2 Decontamination .................................................................................................................... 30
Declaration of Decontamination...................................................................................................... 31
4.3 User maintenance ................................................................................................................... 32

Introduction
Instruction Manual, Sep-18 2
4.4 Service Maintenance ............................................................................................................... 33
4.5 Troubleshooting...................................................................................................................... 33
Change of X/Y drive battery............................................................................................................ 33
Change of extraction head.............................................................................................................. 33
Fittings and capillaries ..................................................................................................................... 34
Installing high pressure fittings........................................................................................................ 34
Fittings – dead volumes................................................................................................................... 34
Fittings – System leaks .................................................................................................................... 35
Exchange of capillaries .................................................................................................................... 37
5Technical data............................................................................................................................... 37
6Appendix A: Maintenance Data Sheet....................................................................................... 38
EC - Declaration of Conformity

Introduction
Instruction Manual, Sep-18 3
1Introduction
1.1 Intended use of the equipment
The main function of the DBS-MS500 is the fully automated solvent extraction of dried
blood spot samples for detection by a mass spectrometer. The samples are delivered on
cards, with up to four spots per card. To ensure reliable automated sample extraction and
handling, these cards are stored in removable racks which hold them in exactly defined
positions inside the instrument. The instruments handling module picks cards out of the
rack and carries them to an image processing system and then to an optional station for
the application of the internal standard before delivering the card to the extraction unit.
Finally, the sampled cards are stored back in the rack position they were picked from.
With the image processing system, multiple quality factors and identification features of
each card will be measured and checked to ensure the correct spot is sampled and that
this spot has not been incorrectly spotted, or previously sampled. The exact positioning of
the card in the extraction unit will be made according to these values. The optional IS
working solution will be applied at the internal standard station to only the sample to be
extracted. At the extraction unit the analyte will be eluted out of the card using the
preferred solvents (specific extraction solvent mixture). This extract will then be delivered
to the HPLC-MS system for further separation and detection as required. Handling and
extraction processes are to be designed for maximum robustness and minimum carryover.
1.2 Basic overview
Fig. 1: The DBS-MS 500, Overview of functionalities

Introduction
Instruction Manual, Sep-18 4
1. Pick up DBS cards (Robotic-module)
Highly reliable robotic are used for handling of DBS cards. The cards (type: ID Biological
Systems or Whatman) are stored in up to 5 racks each with a capacity of 100 cards. The
robotic include a newly developed griping tool specifically adapted to DBS cards. The
gripping tool picks up cards and moves them to all of the subsequent processes in the
corresponding modules.
2. Optical card recognition (OCR-module)
A digital camera with adapted sensor determines the cards properties: spot number and
position, checkbox status and barcode information. The DBS card is only accepted for
analysis if all parameters comply. CAMAG has incorporated its knowhow of more than a
decade of optical HPTLC image analysis to build this precise and reliable OCR-module.
3. Internal standard application (ISA-module)
Variability in the absolute response of LC-MS systems can now be corrected by applying
an internal standard solution onto DBS cards directly before the analysis. This also allows
correcting for extraction efficiencies. The application of the internal standard just before
the extraction of the DBS card is much easier from a logistic point of view than adding the
internal standard before the blood sample is spotted onto the DBS card. CAMAG has
been manufacturing high precision application instruments for HPTLC for a long time and
has used this experience to build the ISA-module.
4. Extraction of the DBS card (Extraction-module)
The precise extraction process involves a sample loop which is independent from the LC-
MS system, thus allowing for high flexibility for differ fluid configurations. Whether you
are using a standard sample loop of 20µL or want to flush 200µL of solvent through a
trapping column – the system can be configured according to your needs, just like your
usual LC-MS system.
5. Wash station (extraction –module)
Requirements regarding carry-over limits are a challenge, particularly for direct sample
loop filling without a trapping column. Therefore a designated wash station cleans all
parts that are in contact with the DBS cards and additionally flushes the entire tubing to
avoid any carry-over. Using this system, independent user tests confirm that carry-over is
not an issue.
6. Replace DBS cards
Finally, the robotic gripping tool puts the cards back into their original position in the rack.
Depending on user requirements there are additional options, e.g. verification of DBS
cards for proper extraction or further tests depending on future requirements.

Introduction
Instruction Manual, Sep-18 5
1.3 Fluidic principle
Fig. 2 shows the fluidic principle diagram with loop valve (V2) in position “Elution”. The
Elution pump presses the elution solvent through the ambos and DBS card into the DBS-
MS500 loop (connection 3 and 8 of loop valve). Meanwhile the flow of the LC pump is
bypassed (through connection 4 and 7).
Fig. 2 Fluidic principle diagram in position “Elution”
Fig. 3 shows the fluidic principle diagram in position “Inline”. The LC pump generates a
steady flow of LC solvent. This transports the elution solvent from the DBS-MS500 loop
into the MS system. Meanwhile, the rinsing pump is connected through the port 10 and 9

Introduction
Instruction Manual, Sep-18 6
to ambos. The rinsing unit is placed in-between the ambos and the extraction head. The
rinsing solvent is cleaning the ambos and extraction head.
Fig. 3 Fluidic principle diagram in position “Inline”
1.4 Precautions
•Please read this operating manual before starting the installation! This manual
contains information and warnings the user has to follow to ensure reliable
operation of the instrument
•Some interior parts of the instrument are under AC power. Careless and improper
use can cause injury. Unauthorized manipulations can cause damage

Introduction
Instruction Manual, Sep-18 7
•If the instrument is used in a manner not specified in this manual, the protection
provided by the equipment may be impaired
•This sign indicates (on instrument and in this manual) that failure to take note of
the accompanying information may result in damage of the instrument
•The instrument is manufactured and tested in accordance with the respective
European safety publications shown on the Declaration of Conformity (DoC). The
instrument complies with safety class 1 and has been designed for indoor use only
(IP 20). Further, this device has passed the CAMAG Quality Assurance tests and has
been delivered in safe operation condition. For detailed instrument data see chapter
technical data
•Attention: For safety reasons the instrument may only be used for the purposes
described in the operating manual
•To avoid injury use adequate safety equipment (protective goggles, gloves etc. if
applicable) when working with the instrument
•Before first operation, check whether the voltage shown on the instrument
matches your local mains voltage. The power cord may only be connected to a
grounded, fused (not higher than 16A) outlet. Do not use extension cords without
ground contact
•When working with the fluids of the instrument, be sure to take the appropriate
caution (protect your eyes from direct contact with liquid)
•Risk of finger squeeze between moving parts; be careful closing the door
•The instrument may be used only by properly trained laboratory staff
•The instrument may not be used in rooms with danger of explosions
•The instrument contains highly sophisticated electronics and optical parts. It may be
operated only in a non-condensing atmosphere in the temperature range outlined
in the chapter "Technical Data". Before installation and use, the instrument should
be acclimated properly
•Use a damp lint free cloth for cleaning the instrument surface. Do not employ
aggressive detergents
•Protect yourself and the instrument from electrostatic shock which can cause
damage to the electronic parts
•Only authorized personnel may open the instrument. Service and repair is only to
be performed by trained specialists. Use spare parts and consumables supplied by
CAMAG only. The warranty is voided if parts from other sources are used. Check
the service manual before you start service to reduce product-specific risks
•The power cord has to be removed before the instrument is opened. It is not
permitted to work on an instrument that has been opened and is connected to the
power supply

Introduction
Instruction Manual, Sep-18 8
•Spare fuses must be of the type specified by the instrument manufacturer. It is
forbidden to short-circuit or manipulate fuses
•Use only the original, with the instrument delivered power cord type
•If the instrument is found to be defective, it must be switched off and steps must
be taken to ensure that it cannot be switched on by mistake
•If liquids penetrate the inside of the instrument, the power has to be disconnected
immediately. Small amounts of liquid can be wiped off and/or dried by means of a
hairdryer, with larger amounts of liquid a service technician has to be called. A test
of functionality has to be performed in all cases
•Carry out all safety checks and the preventive maintenance as recommended by the
manufacturer in order to assure your personal safety and the full functionality of
the instrument. Have an authorized service specialist perform any service not
described by this manual
•See original manufacturers' manuals for further safety data on third party
equipment supplied with the system
•Lift/move/transport the system with the necessary care and with sufficient
manpower (install the transport security devices if applicable, transport it only in the
original packaging)
•The safety of any system incorporate with the equipment is the responsibility of the
assembler of the system
•When working with analytical instrumentation, you must know the potential
hazards of using chemical solvents. Many organic solvents, mobile phases and
samples are harmful to health. Be sure that you know the toxic and infectious
properties of all substances you are using
•This sign draws attention to the potential risk of injury due to hot surfaces. Allow
to cool down before touching!
•This sign draws attention to the potential risks due to pathogenic or genetically
modified organisms and serves to reduce the dispersal and uncontrolled
multiplication of organisms and to protect people from unintentional infection. If
handling with such substances be aware of the applicable guidelines
•This symbol draws attention to the fact that this equipment must not be disposed
of as unsorted municipal waste but is to be collected separately as electrical and
electronic equipment (WEEE-Directive 2002/96/EC). To properly recycle the
instrument or parts of it you are requested to send the equipment back to the
distributor, producer or an adequate collection system at the end of its life. This will
have potential effects on the environment and human health

Introduction
Instruction Manual, Sep-18 9
1.5 Parts supplied
DBS-MS 500 basic, parts supplied with the instrument
Part no Units Reference
050.0705 1 Chronos Software license
110.5115 1 HMI (user interface)
115.5139 1 Waste canister 10L GL45 including tube/filter
115.5110 5 Rack
125.1035 1 Cable to HMI
125.5152 1 Contact closure cable
305.00xx 1 Power cord
666.0008 2 Fitting Peek long
666.0029 5 Solvent filter solvent/rinsing bottles
672.0002 3m Tubing Polyurethane (to compressed air)
672.0029 1m Tubing PVC (to waste canister)
672.1016 2 Tubing clamp
663.1002 1 Seal
720.3095 1 Tubing ferrule
735.3008
2
Vat for solvent bottles
960.0062 2 Bottle for extraction/rinsing 500ml
960.0088 2 Bottle for rinsing 1000ml
960.0087
4
Bottle for internal standard (IS) 25ml
960.0064 1 Bottle for rinsing IS 250ml
B.050.0500E 1 Instruction manual
804.0500 1 Unpacking the DBS
804.5101 1 Entfernen der Transportsicherung D/E
For spare parts and replacement cycles, check the Maintenance Data Sheet
(attachment A)
1.5.1 Optional Parts
DBS-MS 500 basic, parts supplied with the instrument
Part no Units Reference
050.0590 1 Customer Suitcase
050.0591 1 Maintenance Suitcase

Unpacking/Installation
Instruction Manual, Sep-18 10
2Unpacking/Installation
2.1 Unpacking
Check with reference to the parts supplied section whether any parts are missing.
Lift the instrument carefully out of its packaging.
2.2 Installation environment
The place for installation should meet the following requirements:
Connections 115 / 230V AC; 50 / 60Hz; min. 130W; A grounded outlet
should be within 2 meters of the instrument
Contact closure connector
HMI connection
Compressed air 5,5 - 8 bar; 6mm tube (outer diameter)
Connection from LC pump/ to LC/MS system (as short as
possible)
Waste canister connecting tube
For Chronos:
PC with one additional Ethernet port
Bench
specification
Width: 1260 mm (including handles)
Depth: 800 mm (add 250 mm at the rear for tubing and
connections, add 450 mm in front to open the door)
Height: 660mm (plus solvent bottles and capillaries)
Plus adequate space for the waste canister underneath the
instrument
Place the instrument at a solid desk with at least 150Kg
load-carrying capacity
Operating
temperature
The temperature should be within a range of 15 to 30° C
and free of significant variations.
Humidity Humidity and temperature must not cause condensation
Atmospheric
conditions
Adequate ventilation free of acidic, alkaline or other gas
that may corrode metal or painted surfaces must be secured
PC
specification
for Chronos
Windows XP SP3, Vista or Windows 7
At least 4GB RAM
Microsoft .NET Framework 4.0 has to be installed
Additional network connection to DBS-MS500
Use only the original power cord delivered with the instrument.
The power cord has to remain accessible at all time.
Add adequate space for the user interface next to the instrument.
Add adequate space in front and above the instrument to open the door.

Unpacking/Installation
Instruction Manual, Sep-18 11
Other requirements:
•Do not place the instrument in a location where the temperature undergoes
significant changes (e.g. under an air conditioning duct or by a window)
•Significant changes in temperature will affect the performance of the unit
•Do not place the instrument in direct sunlight. Direct sunlight may create significant
temperature changes which will affect the performance of the system
•Do not expose the instrument to any strong vibration or shock
•Avoid placing the instrument near equipment that radiates heat. Do not place the
instrument near gas burners, electric heaters or ovens
•Do not place the instrument near equipment that generates intense magnetic fields
such as electric welding equipment, high frequency furnaces, pole transformers,
etc.
•Protect the instrument from excessive dust
•Connect the instrument to power lines that are free from sudden changes or
voltage fluctuations
•If you must use power motor driven equipment (such as a stirrer or shaker) in the
same line as your instrument, ensure that a noise reduction unit is in the same
power line
2.3 Removal of shipping protection
1. Loosen the cable tie
2. Remove the spring of the gripper arm
3. Remove the 2 knurled screws and the gripper arm holder
4. Remove the 3 knurled nuts and move the plate of the shipping protection to the left

Unpacking/Installation
Instruction Manual, Sep-18 12
5. Move the plate of the shipping protection to the front and
6. Mont the spring of the gripper arm

Unpacking/Installation
Instruction Manual, Sep-18 13
2.4 Installation of the instrument
•Mount the 5 racks (insert the backside of the racks first)
Fluidics
Empty the waste canister on a regular base, if you refill the extraction/rinsing
bottles check the solvent level of the waste canister.
Make sure all bottles are clearly and correctly labelled with their content.
CAMAG strongly recommends using degassed solvents only to reduce micro-
bubbles in the system.
•Connect the waste canister according below figure
Fig. 4: Waste canister connection
•Fill and connect the solvent bottle 1 (500ml, tube “Elution 1” and “E1/R3”) with
your appropriate elution solvent
•Fill and connect the solvent bottle 2 (500ml, tube “Elution 2” and “E1/R3”) with
your appropriate elution solvent
•Fill and connect the rinsing liquid bottle 1 (1000ml, tube “Rinsing R1”) with your
appropriate rinsing solvent
•Fill and connect the rinsing liquid bottle 2 (1000ml, tube “Rinsing R2”) with your
appropriate rinsing solvent
•If applicable, fill and connect the internal standards (IS) bottles (25ml, IS1, 2, 3, 4)
with your appropriate standard solution
•Fill and connect the IS rinsing bottle (250ml) with your appropriate solution
•Connect the external system pump with 10-32 fittings to Valve3, port 4; according
fig. 5 and 6
•Connect the MS with 10-32 fittings to Valve 2, port 7; according the below figure
CAMAG strongly recommends keeping the connection as short as possible (no
longer then 2m) to maintain measurement accuracy and quality.

Unpacking/Installation
Instruction Manual, Sep-18 14
Fig. 5: Fluid Sel F-V1 /Loop F-V2/ CDM F-V3 valvs
Port 4: From LC Pump
Port 7: To LC/MS system
Fig. 6: Loop valve F-V2 connection scheme

Unpacking/Installation
Instruction Manual, Sep-18 15
Electrics
Fig 7. Electric connection panel
•Connect the HMI with the instrument (red)
•Connect the air or Nitrogen pressure (>5.5 bar up to 8 bar) with a 6mm outer
diameter hose (purple). Make sure you keep the length of the hose as short and
the diameter as big as possible
•Connect the required contact closure according table 1 (green, additional
information about the contact closure you can find in the environmental
specification of the instrument) to your measurement system control
Pin Signal Description
1 NC
Relay 1; Loop Inline
(Start signal to LC/MS)
2 NO
3 COM
4 NC
Relay 2; DBS Ready
(DBS-MS500 is ready)
5 NO
6 COM

Unpacking/Installation
Instruction Manual, Sep-18 16
7 IN+ Opto 1; LCMS Ready
(LC/MS is ready)
8 IN- (GND)
9 IN+ Opto 2; Start NxtExtr
(DBS-MS500 can start next extraction)
10 IN- (GND)
11 +24V Power
12 GND Power
Table 1: Contact closure connector
Legend: NC = normally closed
NO = normally open
IN+ = optical input positive (24V)
IN- = optical input ground (GND)
•Connect the power cord (use only the original delivered CAMAG power cord)
•Connect the LAN cable to the computer (for use of CHRONOS software)
•Switch on the unit
2.5 Priming
Prior to first use or after a longer period of non-using the instrument, the fluidic has to be
filled with the respective solvents (Priming).
Priming of extraction circuit
•Choose “Manual”
•Choose “Extractor”
•For the extraction pump (Prime EX):
If the system was completely empty, priming can take up to 50 cycles
•For the rinsing pump (Prime Ri): Activate the pump for about 60 sec.
If the system was completely empty, priming can take up to 5 min.

Unpacking/Installation
Instruction Manual, Sep-18 17
Priming of IS circuit
•Choose “manual”
•Choose “Internal Standard”
•Choose the appropriate IS Bottle
•Set the no of Prime Cycles to 5
•Set the destination parameter to “to waste”
•Activate the priming
•Set the destination parameter to “to Needle”
•Activate the priming
•Repeat the steps above until no more air bubbles are visible in the IS syringe
Priming of IS cleaning circuit
•Choose “manual”
•Choose “Internal Standard”
•Set the time parameter to “5’000” (ms)
•Activate the priming by clicking the “cleaning” button
•Repeat the steps above until a nice and constant jet of IS cleaning solvent is visible
At the priming stage, watch out for any signs of leakage at the fluidic part of the
instrument.
Your system is now ready to use!

Getting started
Instruction Manual, Sep-18 18
3Getting started
3.1 The HMI (Human-Machine interface)
The HMI allows you to control and set all parameters of the DBS-MS500.
Main
Screen
0
Method
Editor
4
Description/
Company/
Name
Sample list Sample
positon
parameter
Internal
Standard IS
Parameters
Extraction Elution
parameters
Rinsing
Parameters
Image
parameters
Image
Processing
Manual
(Functions)
6
Extractor
EX/Rinsing
Priming
Internal
Standard
IS Priming /
Cleaning
Park
Test
Function
Service
Functions
7
Password
I/O Signals
7.1
Adjust
Kinematic
7.2
Adjust
Camera
Import
Parameter
Parameters
7.5
Info
Help
Extended
Settings
7.4
Active Not active
for user
Switch on Setup
Screens
8
Start
Method
1
Pause
Abort
Export
Parameter
Close
Save
Save as
Open
(Methode)
3
Method
1….x
Close
Home/
PgUp/
PgDn/End
Delete
General
Setup
Screen
Refresh
Open (Add)
Auto Adjust
7.6
Start
Stack
2
LC-MS time
Custome
card Close
Stack Editor
5
Close
Save
Add Method
Remove
Fig. 8: Overview of HMI menu structure

Getting started
Instruction Manual, Sep-18 19
3.2 Setting up an extraction with HMI support
•Mount the DBS cards (upside down, front side with DBS spots facing towards the
back of the instrument) to extract into the racks (in a continuous way)
Fig.9: DBS card (front side, upside up)
•Switch the instrument on. Wait until the booting process has ended, the white light
goes on and on the HMI the main screen is displayed
Fig.10: Main screen of HMI