CAMAG ADC 2 User manual

ADC 2
1. Introduction
1.1. CAMAG ADC2
The Automatic Development Chamber (ADC2) permits repro-
ducible chromatogram development without the need for con-
stant monitoring by the user. The ADC2 is shipped with
EquiLink, RS232 cable and built-in interface for connecting to a
computer with winCATS from which the instrument can be run
but it can be used in Stand-Alone mode too.
By means of ADC2, developing of HPTLC chromatograms will
be reproducible. The user is free from any process monitoring.
The user decides whether and how to precondition the plate,
pre-selects the distance to be migrated by the solvent system
and the drying parameters.
Development proceeds automatically and under controlled con-
ditions. A CCD-sensor monitors the progress of the solvent
front; the distance covered and the time elapsed are continu-
ously displayed. On completion of development, the HPTLC
plate is dried with air and finally stays safely protected in the
chamber until removed by the user.
If used in Stand-Alone mode all method parameters are en-
tered via the keypad. Ten complete development programs can
be saved in nonvolatile memory and started at will without PC-
control.
HPTLC plates with a thickness of up to 2.5mm can be used in
the ADC2. If using aluminum foils it is mandatory to utilize a
special foil holder # 115.8363.
The ADC2 controlled by the software winCATS >Planar Chro-
matography Manager< permits full control of the development
process by monitoring and reporting all parameters including all
TLC steps of an analysis, i.e. definition of plate material, sample
application, derivatization, development and evaluation. All data
are handled in a GMP/GLP compliant environment. With the
appropriate winCATS option, the ADC2 can be used in a 21
CFR Part 11 compliant environment.
Information on how to install the ADC2 EquiLink is available in
the comprehensive electronic help system and the interactive
tutorial supplied with winCATS.
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ADC 2
1.2. Precautions
Warning, consult accompanying documents!
This operating manual contains information and warnings that
the user has to follow to ensure reliable operation of the instru-
ment. It has to be read before installation can be started.
The CAMAG ADC2 is manufactured and tested in accordance
with the European safety publications EN 61010-1 : 2001, EN
61326-1: 1997 und EN 61326: 1997/A1: 1998. The instrument
complies with safety class 1 and has been designed for indoors
use only (IP 20). Further, this device has passed the CAMAG
Quality Assurance tests and has been delivered in safe opera-
tion condition.
Before first operating of the instrument, check whether the volt-
age written on the instrument label is the same as that of the
power supply. The power cord may only be connected to a
grounded outlet. Do not use extension cords without ground
contact.
Instrument data:
Mains voltage 100 - 240 V
Frequency 50 / 60 Hz
Power consumption 20 W
)Some internal parts of the instrument are under AC power, un-
authorized manipulations can cause damage.
•The ADC2 must only be used by properly trained laboratory
staff.
•It’s not allowed to work with ADC2 in rooms with danger of
explosions.
•The instrument contains highly sensitive electronic and me-
chanic parts; it should only be used in dry rooms at tempera-
tures between 18°C and 35° C. Before installation and use,
the instrument should be acclimated properly.
•Attention: Some solvent used as mobile phase may be
highly flammable. Please exercise the necessary caution
(e.g. keep away any hot items)!
•If small volumes of liquid have entered the instrument it must
be unplugged immediately. After drying (for instance with a
hairdryer) the functionality of the instrument must be checked
prior to further use. If large volumes of liquid have entered
the instrument, the CAMAG service must be notified.
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ADC 2
•Use a humid cloth for cleaning the instrument surface. Do not
employ aggressive detergents.
•Protect yourself and the instrument from electrostatic shock,
which can cause damage to the electronic parts.
•Only authorized personnel may open the instrument. Service
and repair is only to be performed by trained specialists. Use
spare parts and consumables mentioned in the manual only.
The warranty is voided if other parts are used.
•The instrument must be unplugged before opening. It is not
permitted to work on an instrument that has been opened
and is connected to the power supply.
•Replacement fuses must meet the specifications of the in-
strument manufacturer. Shorting or manipulating fuses is for-
bidden.
•If safety is impaired, the instrument must be switched off and
secured in a way that prevents it from being switched on ac-
cidentally.
•Shipping the instrument is permitted only in the original con-
tainer. Improper handling or packing can cause damage to
the instrument.
•Carry out all safety checks and the preventive maintenance
as recommended by the manufacturer in order to assure
your personal safety and the full functionality of the instru-
ment. Have an authorized service specialist to perform any
service not described by this manual.
This symbol indicates that this equipment must not be dis-
posed as unsorted municipal waste but is to be collected
separately as electrical and electronic equipment (WEEE-
Directive 2002/96/EC). To correctly recycle the instrument or
parts of it you are requested to send the equipment back to
the distributor, producer or an adequate collection system at
the end of its life. This will have potential effects on the envi-
ronment and human health.
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1.3. Parts supplied
Part # Parts supplied
022.8350 ADC2 Device for 100 – 240 V, 50 / 60 Hz, 20W
140.8350 Tool kit, comprising:
022.5261Twin Trough Chamber
672.0059Exhaust tube
115.8352Plate holder(old)-Newmagnetholder#022.8372
941.0006 Saturation pads (pack of 25)
125.1021RS232 connecting cable
362.0006Fuses 1AT (2 pc)
705.0012Allen wrench 3mm
960.0200 Cap for Inlet (2 pc)
305.0015 Power cord CH-plug or
305.0016 Power cord EURO-plug or
305.0017 Power cord USA-plug or
305.0031 Power cord GB-plug
027.6355 ADC2 – EquiLink to winCATS
B.022.8350-E Manual (English)
1.4. Options
Part # Description
022.8360 Humidity Control Module
115.8363 Foil holder (old) - New magnet holder #022.8373
125.1026 Ethernet cable 3m gray
125.1027
105.8352 Ethernet cable 3m red crossed Additional tank komplete for humidity control
1.5. Spare parts
Part # Description
022 5251 Twin Trough chamber
022.8370 Saturation pads (pack of 100)
115.8352 Plate holder(old) - New magnet holder #022.8372
115.8363 Foil holder (old) - New magnet holder #022.8373
672.0059 Exhaust tube
022.8375 Filters for Humidity Control Module (pack of 10)
960.0200
672.0058 Cap for Inlet Connecting Sleeve for exhaust or humidity linking hose
(see page 9 and 35)
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ADC 2
2. Installation
2.1. Instrument
)Observe the environmental requirements (2.2 Installation envi-
ronment) when setting up the instrument.
9Carefully unpack all components and accessories listed on
the shipping list from the upper part of the packing. Make
sure the shipment is complete.
9Remove the instrument carefully from the package and
place it on a table.
Attention: Do not pull out the instrument by holding it at the
blue top-cover; hold the instrument at the bottom when tak-
ing it out of the package and carrying it around!
9Remove carefully the fixation under the Drying module.
9For shipment, the Twin Trough Chamber has been securely
positioned below the bottom of the instrument.
Fig. 1: The ADC2 instrument
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ADC 2
Fig. 2: The instrument ADC2 (opening the front door)
2.2. Installation environment
The place for installation must meet the following requirements:
Bench space Width 320mm
Depth 320mm without Humidity Option
Depth 460mm with Humidity Option
(additional for tubes 250mm)
Height 520mm
Weight 18.5kg
Operating
temperature The temperature should be within a range of 18 to 30 degrees centigrade and free from significant varia-
tions.
Humidity Humidity and temperature conditions must not cause
condensation.
Atmospheric
conditions Adequate ventilation free from acidic, alkaline or other gas that may corrode metal or painted surfaces
must be secured.
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Further requirements:
•Do not place the instrument in a location where the tempera-
ture significantly changes (e.g. under an air conditioning duct
or by a window). Significant changes in temperature will af-
fect the performance of the unit.
•Do not place the instrument in direct sunlight. Direct sunlight
will disturb the measurement of the CCD sensor.
•Do not use the instrument in an environment with moving
ambient air (draft).
•Do not expose the instrument to any strong vibration or
shock.
•Avoid placing the instrument near equipment that radiates
heat. Do not place the instrument near gas burners, electric
heaters or ovens.
•Do not place the instrument near equipment that generates
intense magnetic fields such as electric welding equipment,
high frequency furnaces, pole transformers, etc.
•Protect the instrument from excessive dust.
•Connect the instrument to power lines that are free from
sudden changes or voltage fluctuations.
•If you must use power motor driven equipment (such as a
stirrer or shaker) in the same line as your instrument, ensure
that a noise reduction unit is in the same power line
2.3. Conditions for the installation
Confirm that the following requirements exist before installing
the instrument:
Power supply and ground
Line voltage: 100 – 240V (see rating plate on instrument).
Frequency: 50 / 60 Hz
Power capacity: 20 W
Ground terminal:
A grounded outlet should be located within 2 meters of the instrument.
Connection for Exhaust tube
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2.4. Mains connection
Use the supplied power cord for connecting the instrument to
the mains outlet.
3
2
1
4
Fig. 3, Connections: 1 = RS232 Interface, 2 = Ethernet, 3 = Humidity module, 4 =
Power switch and mains connection with fuse box.
2.5. Connection to the PC
The CAMAG ADC2 is shipped with a 9-pin RS 232 interface
cable for connection to the PC.
If you wish to use the instrument controlled by winCATS:
9Connect the cable to the instrument and to a free COM-port
of the PC to enable data transfer.
As an option the instrument may also be connected to a net-
work using an Ethernet cable. The necessary cable can be or-
dered from CAMAG (see Options on page 4).
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ADC 2
2.6. Mounting the exhaust tube
The shipment contains an exhaust tube, witch can be slipped
onto the “black” air outlet fig.4, permitting exhaust solvent vapor
to be directed into a fume hood or other vent.
Fig. 4: Mounting the exhaust tube to the air outlet
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Connecting Sleeve
# 672.0058

ADC 2
3. Getting started
3.1. Automatic instrument monitoring
A sensor system controls and monitors the proper handling and
guarantees a monitored developing process. In case of failure a
signal will sound and an error message displayed. For error
messages see chapter 6.8.
3.2. Checking system functions
Switch on the instrument. The ADC2 will now show the installed
firmware version and perform the following checks and tasks:
1. Initializing the drying module.
2. Initializing the lift drive.
If startup was successful the display will show:
** ADC2 **
SYSTEM READY
In this phase, only physical blocking of a drive can cause an er-
ror. If necessary, check whether there are obstacles in the in-
strument.
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ADC 2
4. Manual operation
4.1. HPTLC plate / plate holder / foil holder
The plate-holder is designed to attach and handle the HPTLC
plates in the ADC2. When inserting a plate into the plate holder
the TLC layer side must be facing the user (the text ‘LAYER’).
Fig. 5: Insert TLC plate in plate-holder
Fig. 6: Plate clamped in Plate-holder
Important: After being clamped, the HPTLC plate must be
positioned as shown - stop position (1) and the edges of
the holder (2) do not overlap the glass plate (see fig. 6).
1
2
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ADC 2
Note: Clamping foils works similar to that of HPTLC plates; the
two edges of the foil, left/right, must be inserted underneath the
respective down holders, fig. 7.
Fig. 7: Mounting a foil into a foil-holder
4.2. Drying module and plate lift
To attach the plate or foil holder into the drying-module
approach the holder to the lift magnets as shown in fig. 8.
Fig. 8: Fixation of plate holder into drying module
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ADC 2
4.3. Positioning of Twin Trough Chamber
To insert or take out the Twin Trough Chamber the lever (2)
must be in the upper position, see fig. 9.
When the Twin Trough Chamber has been positioned inside the
ADC2 make sure the chamber is touching the two stoppers at
the back, otherwise chromatography cannot be started. The
‘CAMAG’ label of the Chamber should normally face the user;
strongly inhomogeneous glass walls may thus require turning
the chamber around.
After a correct positioning of the Twin Trough Chamber, turn the
lever down in order to lower the drying module onto the edge of
the Chamber (1).
Fig. 9: 1 = Twin Trough Chamber, 2 = lever
1 2
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4.3.1.Use of saturation pad:
)When a saturation pad is needed, make sure it has been in-
serted in the right position, i.e. the fold of the pad is contacting
the bottom of the Twin Trough Chamber while the shorter side
is lying on the bulge of the chamber, fig. 10.
Fig.10: Positioning of saturation pad
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4.4. Developing and preconditioning solvent
The inlet reservoirs for developing (mobile phase) and precondi-
tioning solvents are located on top of the ADC2. The left inlet is
used for developing solvent and the right inlet for precondition-
ing solvent. The volume of developing solvent should be 10ml,
the preconditioning volume 25ml.
Developing Preconditioning
solvent (10ml) solvent (25ml)
Fig.11: Inlet reservoirs
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4.5. Display and function of keys
Control lamps
The control lamps in the display indicate the following status:
•POWER ON: this lamp is lit when the instrument is switched
on.
•ON LINE: this lamp is lit when the instrument is ON-LINE
with PC / winCATS.
The following keys are available:
DIALOG
Activates the manual parameter input mode (Method stack and
manual functions).
ARROW KEYS 5and 6
Select the next / previous parameter (or method) in the dialog.
)If the “SYSTEM READY” display is active, pressing these keys
will display the actual temperature and humidity. By pressing
another key, these measurements will be cleared.
ENTER
Confirms the current parameter, switches to the selected dialog
or (re-)starts the (last) selected method in manual function.
END
Interrupts the current application or switches back to the previ-
ous dialog.
)If the “SYSTEM READY” display is active, you can enter the
manual function stack.
9
9
•
Select any manual function by arrow keys or .
Press ENTER, to start a manual function or DIALOG, if you
5 6
want to change the manual parameters.
•OQTEST (performs instrument’s functionality tests)
•OQ TEST = SELF TEST (since FW v. 1.01.02)
•HUMIDITY CTRL (performs a timed humidity process)
•DRYING (performs a drying step)
RINSING (performs a rinsing step)
•LAST RESULTS (displays the results of the last run)
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RESET
Resets and re-initializes the instrument. The current method is
interrupted but all parameters remain in memory.
RUN
Shows the selection of stored methods.
9Use the arrow keys 5or 6to select a method to start.
9Press ENTER to start the selected method or DIALOG to
change the parameters of the actual method.
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5. Stand-Alone operation
The instrument can be used in Stand-Alone mode. Up to 10
methods can be stored and used locally. Further you can start
4 permanent programmed methods (S1 to S4).
)Note that locally started methods cannot be used in an analysis
created in winCATS, and that local parameter changes are not
documented in winCATS!
To save a winCATS method in the instrument follow the proce-
dure outlined in the ADC2 section of the winCATS help.
5.1. Procedure for Stand-Alone start:
9Prepare the instrument for development.
9Press the RUN key.
The most recently used method is displayed. With the 5or
6keys you can select another locally stored method.
9Press the ENTER key to start the displayed method. The
last method is re-started if ENTER is pressed in the
“SYSTEM READY” mode.
5.2. Procedure for local input of parameters:
9Press the RUN key.
The most recently used method is displayed. With the 5or
6key you can select another locally stored method.
9Press the DIALOG key to locally change parameters of the
selected method.
Press the 5or 6key to select a parameter group (global
parameters, parameters or save method) for local input and
press ENTER to confirm.
9Now you can step through the parameters of the group by
means of the ENTER key or use the 5or 6key to change
the current parameter. To accelerate the change speed of a
parameter you have to press the other arrow key a little bit
later. Press ENTER to confirm the new value and move on
to the next parameter.
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5.3. Procedure for saving a changed method:
9Select the parameter group Save method or press the END
key after having changed the parameters.
9Select the memory position to save the new method by
means of the 5or 6key. Press ENTER to confirm position
and save the parameters.
5.4. Local delete of a saved method:
9Press the RUN key.
The most recently used method is displayed. With the 5or
6key you can select the locally stored method to delete.
9Press the key combination RUN-END (press and hold RUN,
then press END) and confirm deleting with ENTER.
)Flow diagrams for these dialogs are depicted in chapter 6.5.5. Aborting an application in progress
Procedure:
9Press the END key
The development in progress is aborted and if the plate was
immersed in solvent, a drying process will follow. An aborted
development cannot be continued.
5.6. Resetting the instrument
Procedure:
9Press the RESET key
This will abort all activities in progress and re-initialize the
instrument.
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6. Display texts and diagrams
6.1. Texts and their meaning
Text Description
FW V: 1.00.01
************* Shows the actual firmware
version at startup.
** CAMAG **
* ADC2 SYSTEM * Display during initialization of ADC2
at power up.
** ADC2 **
SYSTEM READY The system was properly initialized
and is waiting for a development.
THIS METHOD ?
METHOD 0-10 Selection of a stored method.
THIS FUNCTION ?
MAN. FUNCTION Selection of a manual function.
WAITING FOR
SOMETHING Device is waiting for closing the door,
moving the lever down or proper po-
sitioning of the Twin Trough Cham-
ber.
INIT
DRIVE Initialization of specified drive.
GO TO
POSITION Lift or spindle drive are moved to
specified position
TEMP. HUMIDITY
+24.9°C 40.1%RH The current temperature and relative
humidity are shown.
‘ACTION’
’REST TIME’ An action is running (for example
drying) and the rest time is shown.
DEVELOPMENT
45.3mm 22:45 Development in process since 22
min and 45s. The migration distance
is currently 45.3mm.
OPERATION
COMPLETED Development has been properly
completed.
** ERROR 38 **
ERROR MESSAGE See chapter 6.8, page 26 for error
messages.
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