Optika Italy SFX Series User manual

Ver. 2.1 2021
Model
SFX-31
SFX-32
SFX-33
SFX-34
SFX-51
SFX-52
SFX-91
SFX-91D
ST-50Led
SFX/STEREO Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 SFX-Series 4
3.2 ST-50LED 5
4. Unpacking 6
5. Intended use 6
6. Symbols and conventions 6
7. Instrument description 7
7.1 SFX-31 7
7.2 SFX-32 8
7.3 SFX-33 9
7.4 SFX-34 10
7.5 SFX-51 11
7.6 SFX-52 12
7.7 SFX-91 13
7.8 SFX-91D 14
7.9 ST-50Led 15
8. Assembling 16
9. Use of the microscope 16
9.1 Placing the specimen 16
9.2 Turning on the light 16
9.3 Adjust interpupillary distance 17
9.4 Focus 17
9.5 Diopter compensation 17
9.6 Magnication 17
9.7 Contrast disc 18
9.8 Inserting the batteries 18
9.9 Charge Battery LED 18
10. Installation of camera drivers and software 19
11. Maintenance 20
12. Troubleshooting 21
Equipment disposal 22

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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3. Package content
3.1 SFX-Series
③
①
②
④
⑤
⑧
① Microscopebody
② Microscopebase
③ Eyepieceshields
④ Rechargeablebatteries(forrechargeablemodels)
⑤ Dustcover
⑥ Frostedglassplate
⑦ Tensionadjustmenttool
⑧ Powersupply/powercord
* OnlyforSFX-91D:USBcable
⑥⑦

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3.2 ST-50LED
③
①
②④
⑤
① Microscopebody
② Microscopebase
③ Eyepieceshields
④ Dustcover
⑤ Tensionadjustmenttool
⑥ Powersupply
⑥

Page 6
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 7
TURNABLE
OBJECTIVE
EYEPIECES
DIOPTER
COMPENSATION
RING
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
TRANSMITTED/
REFLECTED LIGHT
SWITCH
LIGHT
INTENSITY
ADJUSTMENT
FOCUS KNOB
SLIDE CLAMP BATTERY
CHARGE LED
7. Instrument description
7.1 SFX-31

Page 8
TURNABLE
OBJECTIVE
EYEPIECES
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
LIGHT
INTENSITY
ADJUSTMENT
FOCUS KNOB
SLIDE CLAMP
TRANSMITTED/
REFLECTED
LIGHT SWITCH
BATTERY
CHARGE LED
7.2 SFX-32
DIOPTER
COMPENSATION
RING

Page 9
TURNABLE
OBJECTIVE
EYEPIECES
HANDLE
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
TOUCH CONTROL
FOCUS KNOB
SLIDE CLAMP BATTERY CHARGE
LED
7.3 SFX-33
DIOPTER
COMPENSATION
RING

Page 10
TURNABLE
OBJECTIVE
EYEPIECES
HANDLE
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
TOUCH CONTROL
FOCUS KNOB
SLIDE CLAMP
BATTERY
CHARGE LED
7.4 SFX-34
DIOPTER
COMPENSATION
RING

Page 11
TURNABLE
OBJECTIVE
EYEPIECES
HANDLE
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
TOUCH CONTROL
FOCUS KNOB
SLIDE CLAMP BATTERY
CHARGE LED
7.5 SFX-51
DIOPTER
COMPENSATION
RING

Page 12
TURNABLE
OBJECTIVE
EYEPIECES
HANDLE
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
TOUCH CONTROL
SLIDE CLAMP
FOCUS KNOB
BATTERY CHARGE
LED
7.6 SFX-52
DIOPTER
COMPENSATION
RING

Page 13
TURNABLE
OBJECTIVE
HANDLE
INCIDENT LIGHT
STAND
TRANSMITTED
LIGHT
ON-OFF SWITCH
TOUCH CONTROL
FOCUS KNOB
SLIDE CLAMP
BATTERY
CHARGE LED
7.7 SFX-91
DIOPTER
COMPENSATION
RING
EYEPIECES

Page 14
TURNABLE
OBJECTIVE
EYEPIECES
HANDLE
CAMERA USB
OUTPUT
INCIDENT LIGHT
TRANSMITTED
LIGHT
ON-OFF SWITCH
TOUCH CONTROL
FOCUS KNOB
SLIDE CLAMP
BATTERY CHARGE
LED
7.8 SFX-91D
DIOPTER
COMPENSATION
RING

Page 15
OBJECTIVE
EYEPIECES
STAND
ON-OFF SWITCH
FLEXIBLE ARM LED
ILLUMINATOR
FOCUS KNOB
7.9 ST-50Led
DIOPTER
COMPENSATION
RING

Page 16
8. Assembling
FFig. 1ig. 1
FFig. 2ig. 2
No installation procedure is required for these models.
The only thing you need to do is to connect the power supply (or power cable) to the microscope and the wall socket.
9. Use of the microscope
9.1 Placing the specimen
Place the specimen on the microscope stage and lock the
specimen using the slide-clamp if it is necessary.
• Ensure that the specimen is centered over the stage opening.
(Fig.1)
9.2 Turning on the light
• The microscope comes with an electrical illuminator.
1. Insert the plug of the cable into the power socket (or the
power supply jack in the socket).
2. Turn on the switch on the main body and select your light
source. (Fig. 2A-2B)
AABB
FFig. 3ig. 3
For SFX models, repeatedly press the touch button in order to
change the light intensity.
• Depending on the model, the light intensity can be adjusted
with a knob on the right side of the stand. (Fig. 3)

Page 17
9.3 Adjust interpupillary distance
Hold the right and left parts of the observation head by both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen.
• If two circles appear, the interpupillary distance is too big.
• If two overlapped circles appear, the interpupillary distance is
too small. (Fig. 4)
FFig. 4ig. 4
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
9.4 Focus
Rotate the focusing knob to bring the sample in focus. (Fig. 5)
9.5 Diopter compensation
This compensation makes it possible for people with glasses
to adjust the microscope to their eyes and use the microscope
without glasses.
1. Adjust the diopter compensation ring of the right eyepiece
tube until the image of the right eyepiece is clear and sharp.
Repeat the procedure for the left eyepiece. (Fig. 6)
9.6 Magnication
Select the desired magnication by rotating the objective. (Fig. 7)
• Total magnication used can be calculated as:
Eyepiece magnication x Objective lens magnication.

Page 18
9.7 Contrast disc
You can use the black/white disc in order to enhance the contrast
of the image when using the incident illumination.
If you want to look at bright opaque objects, place the disc with
the black side up. (Fig. 8)
FFig. 8ig. 8
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
9.8 Inserting the batteries
(Models with rechargeable batteries)
• Before you start using the instrument, you must insert the
rechargeable batteries.
1. Open the battery cover (located on the bottom of the
instrument) and insert the provided batteries, taking care to
respect the polarities. (Fig. 9-10)
2. When nished, close the battery cover again.
9.9 Charge Battery LED
(Only SFX models)
This LED ① shows the actual charge level of the batteries. (Fig.
11)
LED ON (green): batteries charged
LED OFF (no color): batteries to be recharged.
The status of the LED is independent of whether the power
supply unit is connected or not.
①

Page 19
10. Installation of camera drivers and software
Only for SFX-91D
Operating system requirements: Windows XP, Windows 7, Windows 8 and Windows 10.
• If the operating system of your PC is Windows XP: before the installation of your Camera please check if Service Pack2
is installed. If not, please update your XP from the Microsoft web site.
The software can be downloaded using the QR code printed on the booklet provided with the manual or using the following
link: https://www.optikamicroscopes.com/optikamicroscopes/download/optikaisview/?wpdmdl=69240
1. Open the folder Optika IS View and run OptikaISView Setup.exe.
2. Now ISView icon appears on your desktop.
3. Open the folder Drivers, select the folder dedicated to your Operative System and run all the .exe les.
4. The installer may show warnings about missing Windows certication: ignore such warnings and select Continue.
• Note: do NOT cancel the installation.
5. At the end of the installation process restart the PC if requested.
6. Plug the camera: it will be identied and Windows will recognize the driver which has just been installed. Then ignore
any message about Windows compatibility and select Continue.
• Note: do NOT cancel the installation.
7. System is ready.

Page 20
11. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°-40°C and
a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the included dust cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in OFF position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note:ethanolandetherarehighlyammableliquids.Donotusethemnearaheatsource,nearsparksornear
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.