CORNING Spin-X UF 6 Instruction manual

Cor i g®Spi -X®UF
6a d 20 mL
Co ce trators
Technical Da a and Opera ing Ins ruc ions
For in vitro use o ly.

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Th s d vic s ar not d sign d to fit into rotors that only acc pt round bottom
29 mm x 105mm tub s, .g., Sorvall SS34 or B ckman JA 20.
Operation
1. S l ct th most appropriat m mbran cut-off for your sampl . For maximum
r cov ry s l ct a mol cular w ight cut off (MWCO) at l ast 50% small r than th
mol cular siz of th sp ci s of int r st.
2. Fill conc ntrator with up to maximum volum s shown in Tabl 1 (pag 4). Ensur
scr w closur is fully s at d.
3. Ins rt ass mbl d conc ntrator into c ntrifug (wh n fix d angl rotors ar us d,
angl conc ntrator so that th print d window fac s upwards/outwards).
4. C ntrifug at sp ds r comm nd d in Tabl 2 (pag 5), taking car not to xc d th
maximum g forc indicat d by m mbran typ and MWCO.
5. Onc th d sir d conc ntration is achi v d, (s Tabl s 3a and 3b, pag 5) for guid
to conc ntration tim s), r mov ass mbly and r cov r sampl from th bottom of
th conc ntrat pock t with a pip t.
Desalting/Buffer Exchange
1. Conc ntrat sampl to d sir d l v l.
2. Empty filtrat contain r.
3. R fill conc ntrator with an appropriat solv nt.
4. Conc ntrat th sampl again and r p at th proc ss
until th conc ntration of th contaminating micro-
solut is suffici ntly r duc d. Typically, 3 wash cycl s
will r mov 99% of initial salt cont nt.
Removing the Spin-X® UF Body from the Filtrate Tube
Th sl v (s n from th nd) is oval in cross s ction
(Figur 1). Th tub is round in cross s ction to giv
a tight fit to th sl v . To r l as th tub from th
sl v , you must pinch th tub — to pr ss it into an
oval shap — b for r moving it with a twisting action.
Helpful Hints
1. Flow Rate
Filtration rat is aff ct d by s v ral param t rs, includ-
ing MWCO, porosity, sampl conc ntration, viscosity,
c ntrifugal forc and t mp ratur . Exp ct significantly
long r spin tim s for starting solutions with ov r 5%
solids. Wh n op rating at 4°C, flow rat s ar approxi-
mat ly 1.5 tim s slow r than at 25°C. Viscous solutions
such as 50% glyc rin will tak up to 5 tim s long r to con-
c ntrat than sampl s in a pr dominantly buff r solution.
Introduction
Corning®Spin-X®UF conc ntrators ar disposabl , singl us only ultrafiltration
d vic s with poly th rsulfon m mbran s (PES) for th conc ntration and/or
purification of biological sampl s. Spin-X UF 6 conc ntrators ar suitabl for sampl
volum s of 2 to 6 mL and th Spin-X UF 20 conc ntrators can handl sampl s up to
20 mL. Both products f atur twin v rtical m mbran s for unparall l d sp d.
Th innovativ d sign, as of us , sp d and xc ptional conc ntrat r cov ri s ar th
main f atur s of th conc ntrators.
Storage Conditions and Shelf Life
Spin-X UF conc ntrators should b stor d at room t mp ratur . Th d vic s should
b us d b for th xpiration dat print d on th box.
Chemical Compatibility
Spin-X UF conc ntrators ar d sign d for us with biological fluids and aqu ous
solutions. For ch mical compatibility d tails, r f r to Tabl 4 (pag 6).
Centrifugal Operation
Spin-X UF conc ntrators can b us d in swing buck t or fix d angl rotors acc pting
standard conical bottom tub s. In a singl spin, solutions can b conc ntrat d in
xc ss of 100-fold. Sampl s ar typically conc ntrat d in 10 to 30 minut s with
macromol cular r cov ri s in xc ss of 95%.
Th longitudinal m mbran ori ntation and thin chann l conc ntration chamb r provid
optimum cross flow conditions v n for particl lad n solutions; th c ntrifugal forc
pulling particl s and solids away from th m mbran to th bottom of th d vic .
Macromol cul s coll ct in an imp rm abl conc ntrat pock t int grally mold d
b low th m mbran surfac , th r by liminating th risk of filtration to dryn ss.
Required Equipment
1. C ntrifug with swing buck t or fix d angl rotor (minimum 25°).
Device Carrier Required
Spin-X UF 6 To fit 15 mL (17 mm diam t r) conical bottom tub s
Spin-X UF 20 To fit 50 mL (30 mm diam t r) conical bottom tub s
2. Past ur or standard pip ts for sampl addition. Pip ttors with g l loading tips ar
r comm nd d for sampl r moval from th conc ntrat pock t.
Rotor Compatibility
Please note: Spin-X UF 20 (30 mm x 116 mm) is d sign d to fit into rotors that can
accommodat Corning 50 mL conical bottom tub s, .g., B ckman All gra 25R with
TS-5.1-500 swing-out rotor with BUC 5 buck ts and 368327 adaptors; B ckman
TA-10.250 25° fix d angl rotor with 356966 adaptors; H ra us Multifug 3 S-R with
(H ra us/Sorvall) 75006445 swing out rotor with 75006441 buck ts and adaptors for
Corning 50 mL conical bottom tub s. Figure 1.

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2. Prerinsing
M mbran s fitt d to Spin-X®UF conc ntrators contain trac amounts of glyc rin and
sodium azid . Should th s int rf r with analysis th y can b r mov d by rinsing fill
volum of buff r solution or d ioniz d wat r through th conc ntrator. D cant filtrat
and conc ntrat b for proc ssing sampl solution. If you do not want to us th pr -
rins d d vic imm diat ly, stor it in th r frig rator with buff r or wat r cov ring
th m mbran surfac . Pl as do not allow th m mbran to dry out.
3.Sterilization of Polyethersulfone Membranes
Poly th rsulfon m mbran s should not b autoclav d as high t mp ratur s will
substantially incr as m mbran MWCO. To st riliz , us a 70% thanol solution
or st rilizing gas mixtur .
Technical Specifications
Table 1. Technical Properties
Spin-X UF 6 Spin-X UF 20
Concentrator Capacity
Swing buck t rotor 6 mL 20 mL
Fix d angl rotor 6 mL 14 mL
Dimensions
Total l ngth 122 mm 116 mm
Width 17 mm 30 mm
Activ m mbran ar a 2.5 cm26.0 cm2
Hold up volum of m mbran <10 µL <20 µL
D ad stop volum * 30 µL 50 µL
Materia s of Construction
Body Polycarbonat Polycarbonat
Filtrat v ss l Polycarbonat Polycarbonat
Conc ntrator cap Polypropyl n Polypropyl n
M mbran Poly th rsulfon Poly th rsulfon
*D ad stop volum as d sign d in molding tool. This volum may vary d p nding on sampl , sampl
conc ntration, op ration t mp ratur and c ntrifug rotor.
Table 2. Maximum Recommended Spin Force (xg)
Swing Bucket Fixed Ang e
Spin-X®UF 6
5,000 to 50,000 MWCO PES 4,000 xg 10,000 xg
100,000 MWCO PES 4,000 xg 6,000 xg
Spin-X UF 20
5,000 to 50,000 MWCO PES 5,000 xg 8,000 xg
100,000 MWCO PES 3,000 xg 6,000 xg
Table 3a. Performance Characteristics Spin-X UF 6
(Time to co ce trate up to 30x [mi .] at 20°C a d solute recovery %)
Rotor Swing Bucket 25° Fixed Ang e
Start Vo ume 6 mL 6 mL
Min. Rec. Min. Rec.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES 20 98% 12 98%
10,000 MWCO PES 13 98% 10 98%
30,000 MWCO PES 12 98% 9 97%
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES 18 96% 15 95%
50,000 MWCO PES 17 96% 14 95%
100,000 MWCO PES 15 91% 12 91%
Table 3b. Performance Characteristics Spin-X UF 20
(Time to co ce trate up to 30x [mi .] at 20°C a d solute recovery %)
Rotor Swing Bucket 25° Fixed Ang e
Start Vo ume 20 mL 14 mL
Min. Rec. Min. Rec.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES 23 99% 29 99%
10,000 MWCO PES 16 98% 17 98%
30,000 MWCO PES 13 98% 15 98%
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES 27 97% 20 95%
50,000 MWCO PES 27 96% 22 95%
100,000 MWCO PES 25 91% 20 90%

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Table 4. Chemical Compatibility
(2hour co tact time, compatible pH ra ge pH 1–9)
Corning®Spin-X®UF Concentrators Ordering Information
Cat. No. Description Capacity Membrane Pack Size
431482 Spin-X UF 6 6 mL 5,000 MWCO 25
431483 Spin-X UF 6 6 mL 10,000 MWCO 25
431484 Spin-X UF 6 6 mL 30,000 MWCO 25
431485 Spin-X UF 6 6 mL 50,000 MWCO 25
431486 Spin-X UF 6 6 mL 100,000 MWCO 25
431487 Spin-X UF 20 20 mL 5,000 MWCO 12
431488 Spin-X UF 20 20 mL 10,000 MWCO 12
431489 Spin-X UF 20 20 mL 30,000 MWCO 12
431490 Spin-X UF 20 20 mL 50,000 MWCO 12
431491 Spin-X UF 20 20 mL 100,000 MWCO 12
Ac tic Acid (25.0%) 1
Ac ton (10.0%) 3
Ac tonitril (10.0%) 3
Ammonium Hydroxid (5.0%) 2
Ammonium Sulphat (saturat d) 1
B nz n (100%) 3
n-Butanol (70%) 1
Chloroform (1.0%) 3
Dim thyl Formamid (10.0%) 2
Dim thyl Sulfoxid (5.0%) 1
Ethanol (70.0%) 1
Ethyl Ac tat (100%) 3
Formald hyd (30%) 1
Formic Acid (5.0%) 1
Glyc rin (70%) 1
Guanidin HCI (6M) 1
Hydrocarbons, aromatic 3
Hydrocarbons, chlorinat d 3
Hydrochloric Acid (1M) 1
Imidazol (500 mM) 1
Isopropanol (70%) 1
Lactic Acid (5.0%) 1
M rcapto thanol (10 mM) 1
M thanol (60%) 2
Nitric Acid (10.0%) 1
Ph nol (1.0%) 2
Phosphat Buff r (1.0 M) 1
Poly thyl n Glycol (10%) 1
Pyridin (100%) 2
Sodium Carbonat (20%) 2
Sodium D oxycholat (5.0%) 1
Sodium Dod cylsulfat (0.1M) 1
Sodium Hydroxid 3
Sodium Hypochlorit (200 ppm) 2
Sodium Nitrat (1.0%) 1
Sulfamic Acid (5.0%) 1
T trahydrofuran (5.0%) 3
Tolu n (1.0%) 3
Trifluoroac tic Acid (10%) 1
Tw n 20 (0.1%) 1
Triton X-100 (0.1%) 1
Ur a (8 M) 1
* 1 = acc ptabl , 2 = qu stionabl , t sting advis d, 3 = not r comm nd d.

© 2010 Corning Incorporat d Print d in USA 11/10 POD CLS-AN-115 REV2
Corning and Spin-X are regis ered rademarks of Corning Incorpora ed, Corning, NY.
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