CORNING Axygen Axypet Pro User manual

A Corning Brand
Axygen® Axypet® Pro
Pipettors
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual

Table of contents
1. Product Description .................................................................1
2. Packing............................................................................................2
3. Pipettor Design ..........................................................................2
4. Safety Recommendations .....................................................3
 5. Specications...............................................................................4
6. Pipettor Operation ....................................................................6
7. Operating Instructions ...........................................................8
8. Aspiration and Dispensing Instructions .........................9
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration.................................................. 10
10. Pipettor Maintenance .......................................................... 13
11. Troubleshooting ..................................................................... 20
12. Spare Parts ................................................................................. 22
13. Limited Warranty.................................................................... 23

Instruction Manual | 1
1. Product Description
The Axygen® Axypet® Pro single-channel pipettor is a
volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 0.1 μL to 1000 μL depending on the model.
Axygen Axypet Pro 8-channel and 12-channel pipettors
havebeendesignedformicroplatelling.Thepipettors
enable precise and simultaneous delivery of 8 or 12 preset
volume doses of liquid. These pipettors are produced in four
volume ranges: 0.5-10 μL, 5-50 μL, 10-200 μL, and 30-300 μL.
Nominal Volume
(µL) Cat. No.
Range of
Pipettor
Volumes (µL)
Pushbutton
Color Code
Single-channel Pipettors
2AP-2-P 0.1-2 Red
10 AP-10-P 0.5-10 Red
20 AP-20-P 2-20 Yellow
50 AP-50-P 5-50 Yellow
100 AP-100-P 10-100 Yellow
200 AP-200-P 20-200 Yellow
1000 AP-1000-P 100-1000 Blue
Multi-channel Pipettors
10 (8-channel)
10 (12-channel)
AP-8-10-P
AP-12-10-P 0.5-10 Red
50 (8-channel)
50 (12-channel)
AP-8-50-P
AP-12-50-P 5-50 Yellow
200 (8-channel)
200 (12-channel)
AP-8-200-P
AP-12-200-P 10-200 Yellow
300 (8-channel)
300 (12-channel)
AP-8-300-P
AP-12-300-P 30-300 Green
Axygen pipettors operate on the air-cushion principle
(i.e., the aspirated liquid does not come into contact with
the shaft or plunger of the pipettor). The liquid is drawn
to the disposable tips that are attached to the pipettor.

2 | AXYGEN AXYPET PRO PIPETTORS
2. Packing
The pipettors are delivered with the following:
• Pipettor
• Instruction manual
• Qualitycontrolcerticate
• Calibration key
• Pipettor hanger
• Lubricant (single-channel pipettor)
3. Pipettor Design
A
B
N1
N
E
L
O
D
C
R
HandleShaft assembly
Module
Single- and Mul-channel Pipeors
A. Pipetting pushbutton
B. Volume adjustment knob: Used to set and lock
the preset volume.
C. Shaft: Made of high quality plastic, ensuring high
chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are
ejected sequentially, which reduces the required force.

Instruction Manual | 3
E. Calibration switch: Switches the pipettor from
operating mode to calibration mode (CAL).
L. Volume counter: Four-digit volume counter indicates
the preset volume.
O. Counter overlay: The overlay is printed with the range
of the aspirated volume according to the pipettor model.
N. Ejector pushbutton
N1. Cap: Protects the calibration mechanism.
R. Multi-channel manifold: Contains a set of plungers
  andasetofexible-suspensionshafts,reducing
the required tip attachment force.
Model Idencaon
Pipettor model volume range is printed on the counter
overlay. Additionally, it is printed on the tip ejector bar
in the multi-channel pipettors.
Materials Used
Handle parts:
• Polypropylene (PP)
• Polycarbonate (PC)
• Polyacetal (POM)
• Stainless steel
Shaft and piston area parts:
• Polypropylene
• Polyvinylideneuoride(PVDF)
• Polyphenylenesulde(PPS)
• Polyamide (PA)
• Fluoropolymer elastomer
• Stainless steel
4. Safety Recommendations
Long-term and correct pipettor operation depends mainly
on the method of use. Please read the Instructions for Use
of the pipettor carefully and follow the rules described herein.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.

4 | AXYGEN AXYPET PRO PIPETTORS
NOTE
• The pipettor is designed for the transfer of liquids
only in the tip. Do not draw liquids without the tip
attached. The aspirated liquid should not get inside the
pipettor, as it may damage the pipettor.
• The single use of the tip reduces the risk of contamination
of samples.
• Keep the pipettor clean, avoiding the use of aggressive
substances (e.g., acetone) for cleaning purposes.
• Do not place the pipettor horizontally if there is liquid
in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the instructions for use
or transferred to a service point.
• Ambient operating temperature: -5°C to 45°C
• Ambient storage conditions: -25°C to 55°C
When working with the pipettor:
• Follow general work safety regulations regarding
hazards related to work in the laboratory.
• Take special care when pipetting aggressive
substances.
• Use protective clothing, goggles, and protective
gloves.
• Avoid pointing the pipettor with the tip attached
andlledatyourselforatothers.
• Only use parts and accessories recommended by
the manufacturer.
5.Specications
The pipettor is a high quality instrument which offers
excellent accuracy and precision. The accuracy and
precision (repeatability) of the liquid volume depends on
the quality of pipet tips used. The values for accuracy and
precision shown in the table below were obtained using
Axygen® pipet tips. It is recommended to use these tips
to obtain proper compatibility with the pipettors and proper
parameters of accuracy and precision of the aspirated liquid.

Instruction Manual | 5
Nominal
Volume
(µL)
Testing
Volume
(µL)
Accuracy
(%)
Permissible
Volumes
(µL)
Precision
(%)
Fit to
Tips
Without
Filter
(µL)
Single-channel Pipettors
2
Min. 0.1 ±40.0 0.06 - 0.14 ≤12.0
10
0.2 ±12.0 0.176 - 0.224 ≤6.0
1±2.7 0.973 - 1.027 ≤1.3
Max. 2±1.5 1.97 - 2.03 ≤0.7
10
Min. 0.5 ±4.0 0.48 - 0.52 ≤2.8
10
1±2.5 0.975 - 1.025 ≤1.8
5±1.5 4.925 - 5.075 ≤0.7
Max. 10 ±1.0 9.9 - 10.1 ≤0.4
20
Min. 2±3.5 1.93 - 2.07 ≤1.5
20010 ±1.0 9.9 - 10.1 ≤0.5
Max. 20 ±0.8 19.84 - 20.16 ≤0.3
50
Min. 5±3.5 4.825 - 5.175 ≤1.3
20025 ±1.0 24.75 - 25.25 ≤0.5
Max. 50 ±0.8 49.6 - 50.4 ≤0.3
100
Min. 10 ±3.0 9.7 - 10.3 ≤1.0
20050 ±1.0 99.5 - 50.5 ≤0.3
Max. 100 ±0.8 99.2 - 100.8 ≤0.2
200
Min. 20 ±2.0 19.6 - 20.4 ≤0.7
200
100 ±1.0 99.0 - 101.0 ≤0.3
Max. 200 ±0.6 198.8 - 201.2 ≤0.2
1000
Min. 100 ±2.5 97.5 - 102.5 ≤0.6
1000500 ±0.8 496 - 504 ≤0.3
Max. 1000 ±0.6 994 - 1006 ≤0.2
Multi-channel Pipettors
10
(8-channel)
10
(12-channel)
Min. 0.5 ±10.0 0.45 - 0.55 ≤8.0
10
1±8.0 0.92 - 1.08 ≤6.0
5±4.0 4.8 - 5.2 ≤2.0
Max. 10 ±2.0 9.8 - 10.2 ≤1.2
50
(8-channel)
50
(12-channel)
Min. 5±4.0 4.8 - 5.2 ≤2.5
20025 ±3.0 24.25 - 25.75 ≤1.2
Max. 50 ±1.6 49.2 - 50.8 ≤0.6
200
(8-channel)
200
(12-channel)
Min. 10 ±5.0 9.5 - 10.5 ≤3.0
200
20 ±3.0 19.4 - 20.6 ≤1.5
100 ±1.5 98.5 - 101.5 ≤0.8
Max. 200 ±1.0 198 - 202 ≤0.6
300
(8-channel)
300
(12-channel)
Min. 30 ±3.0 29.1 - 30.9 ≤2.0
300150 ±1.2 148.2 - 151.8 ≤1.0
Max. 300 ±1.0 297 - 303 ≤0.6

6 | AXYGEN AXYPET PRO PIPETTORS
The accuracy and precision are obtained gravimetrically using
Axygen® pipet tips performing at least 10 measurements
of distilled water at a temperature of 20°C ± 1°C according
to the EN ISO 8655 standard.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.
6. Pipettor Operation
Attaching the Tips
• Attach the tip corresponding to the model on the pipettor
on the shaft (Section 5).
• Position the pipettor vertically when putting on the tips.
• Single-channel pipettors, when attaching the tip, press
thepipettiponrmlyusingaslighttwistingmotion.
This will ensure an airtight seal.
• Multi-channel pipettors, when attaching the tips on the
shafts, press the pipettor against the tips in the rack box
until the shafts retract approximately 1.5 mm into the
manifold. The suspension system of the shafts ensures even
and tight sealing of the pipet tips. The rocking movement
does not have to be performed to seal the pipet tips tightly.
NOTES:
• Do not attach the tips with a swinging movement,
as this may damage the shaft or plunger. Observe this
rule particularly with single-channel pipettors of low
volume range.
✓ ✓✕ ✕
• Never draw liquids directly into the pipettor without
the tip attached.

Instruction Manual | 7
Volume Setting
NOTE: The red calibration switch above the
counter should remain in the lower position
during operation. Otherwise, any change
in volume will result in a loss of calibration
of the pipettor.
A B C
A. To enable volume selection, set the black adjustment
knob to the upper position.
B. To set a certain value, turn the knob. The volume is
indicated by the counter and its value consists of four
digits that should be read from top to bottom. Digits
on the bottom drum represent the scale division for
the pipettor model. See scale intervals in Section 5.
The volume range of the pipettor model is shown on
the counter overlay.
C. After the desired volume has been set, move the knob
down to avoid an inadvertent change of the volume.
Examples of Counter indications
The white mark
separates the
integer part from
the decimal part of
the aspirated liquid
volume in μL.
Volume Ranges 0.1-2 μL 5-50 μL 100-1000 μL
Set Volume (µL) 0.1 μL 5 μL 100 μL
To attain the maximum accuracy, the set volume must be
approached from a higher value by diminishing counter
readings. Before reaching the desired value, reduce the
speed of turning the adjustment knob to not exceed this
value.

8 | AXYGEN AXYPET PRO PIPETTORS
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sampling.
• During operation, the pipettor’s volume setting should be
locked, the black adjustment knob should be in the lower
position.
• The pipettor is equipped with a counter operating mode
switch, the calibration switch located under the CAL sign
on the counter overlay. This switch should only be used
when the pipettor is calibrated (upper position marked
as CAL on the switch).
• Make sure to operate the pipettor slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration. The recommended immersion depths
are given in the table below:
Model Volume Range [µL] Immersion Depth [mm]
0.1-1 ≤1
1-100 2-3
101-1000 2-4
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed
or when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end
of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to
be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
– Press and release the pushbutton slowly and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommended
limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-rinse
the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions which
emit vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
nishingthepipettingoperation.