CORNING Axygen Axypet Pro User manual

A Corning Brand
Axygen® Axypet® Pro
Pipettors
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual

Table of contents
1. Product Description .................................................................1
2. Packing............................................................................................2
3. Pipettor Design ..........................................................................2
4. Safety Recommendations .....................................................3
 5. Specications...............................................................................4
6. Pipettor Operation ....................................................................6
7. Operating Instructions ...........................................................8
8. Aspiration and Dispensing Instructions .........................9
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration.................................................. 10
10. Pipettor Maintenance .......................................................... 13
11. Troubleshooting ..................................................................... 20
12. Spare Parts ................................................................................. 22
13. Limited Warranty.................................................................... 23

Instruction Manual | 1
1. Product Description
The Axygen® Axypet® Pro single-channel pipettor is a
volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 0.1 μL to 1000 μL depending on the model.
Axygen Axypet Pro 8-channel and 12-channel pipettors
havebeendesignedformicroplatelling.Thepipettors
enable precise and simultaneous delivery of 8 or 12 preset
volume doses of liquid. These pipettors are produced in four
volume ranges: 0.5-10 μL, 5-50 μL, 10-200 μL, and 30-300 μL.
Nominal Volume
(µL) Cat. No.
Range of
Pipettor
Volumes (µL)
Pushbutton
Color Code
Single-channel Pipettors
2AP-2-P 0.1-2 Red
10 AP-10-P 0.5-10 Red
20 AP-20-P 2-20 Yellow
50 AP-50-P 5-50 Yellow
100 AP-100-P 10-100 Yellow
200 AP-200-P 20-200 Yellow
1000 AP-1000-P 100-1000 Blue
Multi-channel Pipettors
10 (8-channel)
10 (12-channel)
AP-8-10-P
AP-12-10-P 0.5-10 Red
50 (8-channel)
50 (12-channel)
AP-8-50-P
AP-12-50-P 5-50 Yellow
200 (8-channel)
200 (12-channel)
AP-8-200-P
AP-12-200-P 10-200 Yellow
300 (8-channel)
300 (12-channel)
AP-8-300-P
AP-12-300-P 30-300 Green
Axygen pipettors operate on the air-cushion principle
(i.e., the aspirated liquid does not come into contact with
the shaft or plunger of the pipettor). The liquid is drawn
to the disposable tips that are attached to the pipettor.

2 | AXYGEN AXYPET PRO PIPETTORS
2. Packing
The pipettors are delivered with the following:
• Pipettor
• Instruction manual
• Qualitycontrolcerticate
• Calibration key
• Pipettor hanger
• Lubricant (single-channel pipettor)
3. Pipettor Design
A
B
N1
N
E
L
O
D
C
R
HandleShaft assembly
Module
Single- and Mul-channel Pipeors
A. Pipetting pushbutton
B. Volume adjustment knob: Used to set and lock
the preset volume.
C. Shaft: Made of high quality plastic, ensuring high
chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are
ejected sequentially, which reduces the required force.

Instruction Manual | 3
E. Calibration switch: Switches the pipettor from
operating mode to calibration mode (CAL).
L. Volume counter: Four-digit volume counter indicates
the preset volume.
O. Counter overlay: The overlay is printed with the range
of the aspirated volume according to the pipettor model.
N. Ejector pushbutton
N1. Cap: Protects the calibration mechanism.
R. Multi-channel manifold: Contains a set of plungers
  andasetofexible-suspensionshafts,reducing
the required tip attachment force.
Model Idencaon
Pipettor model volume range is printed on the counter
overlay. Additionally, it is printed on the tip ejector bar
in the multi-channel pipettors.
Materials Used
Handle parts:
• Polypropylene (PP)
• Polycarbonate (PC)
• Polyacetal (POM)
• Stainless steel
Shaft and piston area parts:
• Polypropylene
• Polyvinylideneuoride(PVDF)
• Polyphenylenesulde(PPS)
• Polyamide (PA)
• Fluoropolymer elastomer
• Stainless steel
4. Safety Recommendations
Long-term and correct pipettor operation depends mainly
on the method of use. Please read the Instructions for Use
of the pipettor carefully and follow the rules described herein.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.

4 | AXYGEN AXYPET PRO PIPETTORS
NOTE
• The pipettor is designed for the transfer of liquids
only in the tip. Do not draw liquids without the tip
attached. The aspirated liquid should not get inside the
pipettor, as it may damage the pipettor.
• The single use of the tip reduces the risk of contamination
of samples.
• Keep the pipettor clean, avoiding the use of aggressive
substances (e.g., acetone) for cleaning purposes.
• Do not place the pipettor horizontally if there is liquid
in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the instructions for use
or transferred to a service point.
• Ambient operating temperature: -5°C to 45°C
• Ambient storage conditions: -25°C to 55°C
When working with the pipettor:
• Follow general work safety regulations regarding
hazards related to work in the laboratory.
• Take special care when pipetting aggressive
substances.
• Use protective clothing, goggles, and protective
gloves.
• Avoid pointing the pipettor with the tip attached
andlledatyourselforatothers.
• Only use parts and accessories recommended by
the manufacturer.
5.Specications
The pipettor is a high quality instrument which offers
excellent accuracy and precision. The accuracy and
precision (repeatability) of the liquid volume depends on
the quality of pipet tips used. The values for accuracy and
precision shown in the table below were obtained using
Axygen® pipet tips. It is recommended to use these tips
to obtain proper compatibility with the pipettors and proper
parameters of accuracy and precision of the aspirated liquid.

Instruction Manual | 5
Nominal
Volume
(µL)
Testing
Volume
(µL)
Accuracy
(%)
Permissible
Volumes
(µL)
Precision
(%)
Fit to
Tips
Without
Filter
(µL)
Single-channel Pipettors
2
Min. 0.1 ±40.0 0.06 - 0.14 ≤12.0
10
0.2 ±12.0 0.176 - 0.224 ≤6.0
1±2.7 0.973 - 1.027 ≤1.3
Max. 2±1.5 1.97 - 2.03 ≤0.7
10
Min. 0.5 ±4.0 0.48 - 0.52 ≤2.8
10
1±2.5 0.975 - 1.025 ≤1.8
5±1.5 4.925 - 5.075 ≤0.7
Max. 10 ±1.0 9.9 - 10.1 ≤0.4
20
Min. 2±3.5 1.93 - 2.07 ≤1.5
20010 ±1.0 9.9 - 10.1 ≤0.5
Max. 20 ±0.8 19.84 - 20.16 ≤0.3
50
Min. 5±3.5 4.825 - 5.175 ≤1.3
20025 ±1.0 24.75 - 25.25 ≤0.5
Max. 50 ±0.8 49.6 - 50.4 ≤0.3
100
Min. 10 ±3.0 9.7 - 10.3 ≤1.0
20050 ±1.0 99.5 - 50.5 ≤0.3
Max. 100 ±0.8 99.2 - 100.8 ≤0.2
200
Min. 20 ±2.0 19.6 - 20.4 ≤0.7
200
100 ±1.0 99.0 - 101.0 ≤0.3
Max. 200 ±0.6 198.8 - 201.2 ≤0.2
1000
Min. 100 ±2.5 97.5 - 102.5 ≤0.6
1000500 ±0.8 496 - 504 ≤0.3
Max. 1000 ±0.6 994 - 1006 ≤0.2
Multi-channel Pipettors
10
(8-channel)
10
(12-channel)
Min. 0.5 ±10.0 0.45 - 0.55 ≤8.0
10
1±8.0 0.92 - 1.08 ≤6.0
5±4.0 4.8 - 5.2 ≤2.0
Max. 10 ±2.0 9.8 - 10.2 ≤1.2
50
(8-channel)
50
(12-channel)
Min. 5±4.0 4.8 - 5.2 ≤2.5
20025 ±3.0 24.25 - 25.75 ≤1.2
Max. 50 ±1.6 49.2 - 50.8 ≤0.6
200
(8-channel)
200
(12-channel)
Min. 10 ±5.0 9.5 - 10.5 ≤3.0
200
20 ±3.0 19.4 - 20.6 ≤1.5
100 ±1.5 98.5 - 101.5 ≤0.8
Max. 200 ±1.0 198 - 202 ≤0.6
300
(8-channel)
300
(12-channel)
Min. 30 ±3.0 29.1 - 30.9 ≤2.0
300150 ±1.2 148.2 - 151.8 ≤1.0
Max. 300 ±1.0 297 - 303 ≤0.6

6 | AXYGEN AXYPET PRO PIPETTORS
The accuracy and precision are obtained gravimetrically using
Axygen® pipet tips performing at least 10 measurements
of distilled water at a temperature of 20°C ± 1°C according
to the EN ISO 8655 standard.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.
6. Pipettor Operation
Attaching the Tips
• Attach the tip corresponding to the model on the pipettor
on the shaft (Section 5).
• Position the pipettor vertically when putting on the tips.
• Single-channel pipettors, when attaching the tip, press
thepipettiponrmlyusingaslighttwistingmotion.
This will ensure an airtight seal.
• Multi-channel pipettors, when attaching the tips on the
shafts, press the pipettor against the tips in the rack box
until the shafts retract approximately 1.5 mm into the
manifold. The suspension system of the shafts ensures even
and tight sealing of the pipet tips. The rocking movement
does not have to be performed to seal the pipet tips tightly.
NOTES:
• Do not attach the tips with a swinging movement,
as this may damage the shaft or plunger. Observe this
rule particularly with single-channel pipettors of low
volume range.
✓ ✓✕ ✕
• Never draw liquids directly into the pipettor without
the tip attached.

Instruction Manual | 7
Volume Setting
NOTE: The red calibration switch above the
counter should remain in the lower position
during operation. Otherwise, any change
in volume will result in a loss of calibration
of the pipettor.
A B C
A. To enable volume selection, set the black adjustment
knob to the upper position.
B. To set a certain value, turn the knob. The volume is
indicated by the counter and its value consists of four
digits that should be read from top to bottom. Digits
on the bottom drum represent the scale division for
the pipettor model. See scale intervals in Section 5.
The volume range of the pipettor model is shown on
the counter overlay.
C. After the desired volume has been set, move the knob
down to avoid an inadvertent change of the volume.
Examples of Counter indications
The white mark
separates the
integer part from
the decimal part of
the aspirated liquid
volume in μL.
Volume Ranges 0.1-2 μL 5-50 μL 100-1000 μL
Set Volume (µL) 0.1 μL 5 μL 100 μL
To attain the maximum accuracy, the set volume must be
approached from a higher value by diminishing counter
readings. Before reaching the desired value, reduce the
speed of turning the adjustment knob to not exceed this
value.

8 | AXYGEN AXYPET PRO PIPETTORS
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sampling.
• During operation, the pipettor’s volume setting should be
locked, the black adjustment knob should be in the lower
position.
• The pipettor is equipped with a counter operating mode
switch, the calibration switch located under the CAL sign
on the counter overlay. This switch should only be used
when the pipettor is calibrated (upper position marked
as CAL on the switch).
• Make sure to operate the pipettor slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration. The recommended immersion depths
are given in the table below:
Model Volume Range [µL] Immersion Depth [mm]
0.1-1 ≤1
1-100 2-3
101-1000 2-4
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed
or when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end
of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to
be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
– Press and release the pushbutton slowly and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommended
limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-rinse
the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions which
emit vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
nishingthepipettingoperation.

Instruction Manual | 9
8. Aspiration and Dispensing Instructions
Aspirating Liquid
1. Pressthepushbuttontotherstpositivestop.
Holding the pipettor vertically, immerse the pipet tip
into the sample liquid. The depth to which the pipet
tip is immersed in the sample liquid depends on
the model (for recommended values see Section 7).
If the pipet tip is not immersed to the recommended
depth or if the pipetting pushbutton is rapidly
released, air may enter the disposable pipet tip.
2. Release the pipetting pushbutton slowly and smoothly
to aspirate the sample. Wait one second and then
withdraw the pipet tip from the liquid.
NOTE: Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall
of the vessel at an angle of 10° to 40°. Then, press the
pushbuttonsmoothlytotherststop.Waitonesecond.
4. Press the pushbutton to the second stop to expel any
remaining liquid. Keep the pushbutton depressed to the
very end, and remove the pipettor by drawing the pipet
tip against the inside surface of the receiving vessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector button.
Remember to change the pipet tip whenever a
different type of liquid is to be sampled.

10 | AXYGEN AXYPET PRO PIPETTORS
Aspirating High-density Liquids
When pipetting liquids of higher viscosity or lower surface
tensionthanwater(e.g.,seraororganicsolvents),almof
liquidisformedontheinsidewallofthepipettip.Thislm
cancreateanerror.Asthelmremainsrelativelyconstantin
successive pipetting operations with the same tip, this error
canbeeliminatedbyformingthelmbeforetransferring
therstsample.Thisisdonebyaspiratingasampleand
dispensingitbackintothesamevessel.Now,asthelmis
already formed, all of the following samples will have better
accuracy and repeatability.
This pre-rinsing operation should be repeated when
the volume to be aspirated is changed or when a new pipet
tip is used.
NOTE: Normally the degree of error resulting from heavy or
viscous liquids is negligible if pipetting is done slowly and
carefully. It is most important to give the liquids some time
to react to the change of pressure by holding the pipet tip
in its position for at least 2 seconds after the aspiration and
the blow out stroke.
If in extreme cases, this method of operation does not result
in accurate values, recalibrate the pipettor out in accordance
with Section 9.
After calibration, it is recommended to record the recalibration
and the size of the correction introduced, to facilitate
reverse calibration to the standard liquid.
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration
The pipettors are factory-calibrated using the gravimetric
method with Axygen® pipet tips and distilled water, in
accordance with ISO 8655 guidelines for the maximum
(nominal) liquid volume drawn by the pipettor and for 10%
of the maximum volume according to the values given
(Section 5).
The design of the pipettors enable their recalibration
and adaptation to other pipetting techniques or liquid
properties (i.e., temperature, density and viscosity).
It is recommended to carry out periodic checks of the
operation of the pipettor at least once a year, the period
of operation of the pipettor with no checks depends
on the type of workload (liquids collected), and other
conditions (load intensity and sterilization in the autoclave,
replacement of components) in which the pipettor is used.
Ifduringpipettoroperationyoundthattheaccuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value

Instruction Manual | 11
given in the table in Section 5, the pipettor recalibration
procedure should be carried out.
Recalibration of the pipettor consists in setting on the counter
the value of the liquid volume obtained in weighing.
Recalibration of the pipettor is performed only for one liquid
volume drawn by the pipettor. The recommended volume
for recalibration is 10% of the maximum (nominal) value.
Conditions for Checking the Pipetting Accuracy Parameters
Thepipettingaccuracyisinuencedbysuchfactorsas:
tips used, characteristics of the pipetted liquid (density,
viscosity), or operating conditions (ambient temperature,
pressure).
To determine the accuracy error of the pipettor, the following
conditions should be met:
• Ambient temperature and the temperature of the
pipettor, pipet tips, and liquid should be within the range
of 20°C to 25°C and stabilized during weighing within
±0.5°C.
• Measurements should be conducted using distilled water.
• Balancesensitivityshouldbesuitableforthevolume“V”
to be measured:
Volume Checked
(µL)
Balance Sensitivity
(mg)
0.1≤V≤ 10 0.001
10≤V≤100 0.01
V>100 0.1
• When calculating the liquid volume aspirated by the
pipettor, the values of conversion factor Z [μL/mg] for
distilled water or a liquid with comparable density should
be taken into account. Sample values of this conversion
factor are given in the following table:
Temperature
(°C)
Pressure (kPa)
95.0 101.3 105.0
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
See ISO 8655 for the full table of the value of the conversion
factor Z.

12 | AXYGEN AXYPET PRO PIPETTORS
NOTE: Pipetting should be performed in accordance with
the rules described in Sections 7 and 8.
Checking the Pipetting Accuracy Parameters
• Set the pipettor to the calibration volume.
• Perform 10 aspirations, and calculate their average value
in [mg].
• Calculate the volume in [μL] by multiplying the value
in [mg] by the conversion factor Z [μL/mg].
If the average aspirated volume exceeds the permissible
values of the range given in Section 5, the pipettor should
be recalibrated.
Pipettor Recalibration
Make sure that the pipettor is set to the calibration volume
and the volume adjustment knob is locked.
1. Remove the cap from the ejector pushbutton.
2. Set the calibration switch to the upper
position(CAL)usingtheatendofthe
calibration key.
3. Insert the calibration key with the cross
end vertically into the hole in the ejector
pushbutton, placing it in the counter’s
calibration knob.
4. Turn the key so that the volume indicated
by the counter is equal to the volume
calculated.
5. Remove the calibration key.
6. Set the calibration switch in the
operating position.
7. Fit the ejector pushbutton cap.
8. Repeat the pipetting accuracy check (Section 9: Checking
the pipetting accuracy parameter). If the accuracy error
value is exceeded, repeat the calibration process.
Operating
position
Calibration
position
Calibration key

Instruction Manual | 13
10. Pipettor Maintenance
Depending on the applications and the intensity of use,
the pipettor requires periodic maintenance.
The components most exposed to vapors of aggressive
solutions, such as shaft elements, should be regularly
checked and cleaned.
To properly carry out the maintenance, familiarize yourself
with the design of the shaft assembly shown in Figures 10A,
10B, 10C, and follow the instructions given in this Section.
Do not use sharp tools for pipettor maintenance.
It may cause damage to the device and affect the
user’s safety.
Cleaning
External surfaces of the pipetting pushbutton, the ejector
pushbutton, the handgrip, the shaft nut, and the adjustment
knob may be cleaned using a cloth dampened in isopropyl
alcohol. The remaining parts removed from the pipettor
during pipettor disassembly may be washed with distilled
water or isopropyl alcohol.
NOTE: Before using cleaning agents other than those
recommended by the manufacturer, check the compatibility
charts and consider chemical resistance of the following
plastics:PP,PC,POM,PA,PPS,PVDFusedtoproducethe
pipettor parts.
Dismantling shaft elements of the single-channel pipettor:
• Holding the pipettor handle, slightly unscrew the shaft
assembly and pull the Tip ejector (D) out of it;
• Still holding the handle, unscrew the remaining part
of the shaft assembly from it.
Then proceed according to the pipettor models shown in
Figures 10A-C.
NOTE FOR ALL MODELS:
When inserting the Shaft
or the Shaft Holder into the
Connecting Bush, make sure
that it is inserted properly
totheend.Thensofthe
Shaft/Holder must match the
grooves in the Bush.
Incorrect assembly of these parts may result in damage to
the components.
After cleaning, apply a thin layer of lubricant on the Plunger
(10A) or the Seal (10B, 10C). Lubricant is supplied with each
pipettor.
✓✕

14 | AXYGEN AXYPET PRO PIPETTORS
Single-channel pipettors 0.1-2, 0.5-10, 2-20 µL (Figure 10A)
To check and clean the shaft and the seal, it is not necessary
to dismantle the entire assembly. Unscrew the Shaft (C)
from the Shaft holder (F1) and remove the O-ring (I), and in
the case of pipettor 2 μL, also the Seal (J).
If you need to clean other components:
• Unscrew the Shaft Cap (M).
• Remove from the Plunger and pipetting Spring (G1).
• Remove the Shaft holder (F1) from the Connecting Bush (F).
• Check the condition of components, and clean as
recommended.
• Replace any damaged parts.
• Assemble the components in reverse order.
NOTE: When disassembling, care should be taken so that
the plunger does not pop out under the action of the
pipetting spring.

Instruction Manual | 15
10A
F1
I
J
D
J
I
C
F
M
G
G1

16 | AXYGEN AXYPET PRO PIPETTORS
Single-channel pipettors 5-50, 10-100, 20-200 µL (Figure 10B)
To clean the shaft or the seal, it is not necessary to
dismantle the entire set. Just unscrew the Shaft (C) from the
Shaft Holder (F1).
If you need to clean other components:
• Unscrew the Shaft Cap (M).
• Remove the Piston with the Pipetting Spring (G1).
• Remove the Shaft holder (F1) from the Connecting Bush (F).
• Check the condition of components, and clean as
recommended.
• Replace any damaged parts.
• Assemble the components in reverse order.
NOTE: When disassembling, care should be taken so
the plunger does not pop out under the action of the
pipetting spring.
After replacing the shaft, it is advised to perform a
calibration check of the pipettor.
If the pipettor is operating incorrectly, please check if the
pipettor was reassembled correctly.

Instruction Manual | 17
10B
C
J
D
F
M
G1
F1

18 | AXYGEN AXYPET PRO PIPETTORS
Single-channel pipettors 100-1000 µL (Figure 10C)
• Unscrew the Shaft Cap (M) and slide it off the Piston (G1).
• Remove the Piston with the Pipetting Spring (G1) from
the Shaft (C).
• Clean the parts of the lubricant and examine the
components, especially the condition of the Seal (J),
O-ring (M1) and the interior of the Shaft Cap (M).
• Replace any damaged parts.
• Assemble the components in reverse order.
NOTE: After replacing the piston or the shaft cap, it is
advised to perform a calibration check of the pipettor. In
case of any signs of incorrect operation of the pipettor,
check the correctness of assembly.