CURIOSIS Celloger Mini User manual

www.curiosis.com
Automated live cell imaging system
Celloger Mini
Instruction manual

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Developed and manufactured by CURIOSIS Inc.
Email: [e-mail]
Website: www.curiosis.com
CURIOSIS Inc.
4F, 10 Teheran-ro 38-gil, Gangnam-gu, Seoul 06221, South Korea
Tel: [phone]
Fax: [phone]
Copyright © 2021, by CURIOSIS Inc.
All rights reserved. Published in Korea.

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Table of contents
Package contents
Device layout
Getting started
Installation
Network setup
Control panel guide
Basic operation
1. Device connection
2. Cell monitoring (Setup mode)
3. Details
Focusing
Manual adjustment
Autofocusing
Plate panel
Features on display
Time-lapse imaging
1. Scan position setup (Setup mode)
2. Scanning (Scan mode)
File storage and project name
Time setup
3. View image file (Album mode)
4. Movie maker (Album mode)
Image analysis (Analysis mode)
1. Confluency
2. Growth curve
Safety information
General guidelines
Operating condition
Safety standards
Cleaning and Maintenance
Trouble shooting
Product specifications
Ordering information
Appendix A. Connecting multiple devices
Appendix B. How to insert Vessel or Vessel Holder
Appendix C. Correct focus and light intensity selection
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5
6
13
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14
17
20
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22
23
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24
25
26
27
29
29
30
32
33

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Package contents
Celloger Mini Live cell imaging device package includes the following items.
Item
Quantity
CELLOGERTM MINI main device
1
Main power cable
1
PoE
1
LAN Cable (5M, 4.8M)
2
USB memory
1
-Quick manual
1
-IP Setting guide
1
-Celloger App
1
Vessel holder for dish 60mm (Optional)
1
Vessel holder for dish 35mm (Optional)
1
Vessel holder for T-flask 25cm2 (Optional)
1
When receiving the package,
• Check that all items listed above are included in your package.
• Examine the device carefully for any damage during shipping.
• Contact your local distributor or inf[email protected] if any items are missing or damaged.
• Any loss or damage claims must be filed with the carrier.

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Device layout

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Getting started
Installation
1. Remove the product from the box.
When taking out the device from the box, ensure to hold the bottom of the device. Brightfield lamp is
located at the center of the device and it is not a handle.
2. Take off the lens cover (black).
3-1. Connect to power cable (Previous version).
A. Plug the power cable into device and connect to PoE (P+ DATA OUT / Right side)

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B. Connect the device to PC using the connection cable and PoE (DATA IN / Left side)
3-2. Connect to power cable (Current version).
A. Plug the power cable into PoE
B. Connect the device to PC using the connection cable and PoE

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Network setup
1. Click the “Start” button or the “Window” icon.
2. Click the “Setting” button.
3. Click the “Network & Internet” button.
4. Click the “Ethernet” button.
5. Click the “Network and Sharing Center” button.
6. Click the “Change adapter settings” button on the left menu.
7. Click the “Ethernet” button and right-click the mouse and click “Properties” in the window that appears.
8. Select “Internet Protocol Version 4 (TCP/IPv4)” and click “Properties”.
9. Select “Use the following IP address” and input “IP address” and “Subnet mask”.
Note: “IP address” should be 192.168.2.##, and in ## field, input 2~254 except 10.
For “Subnet mask”, input 255.255.255.0.
The PC IP address should be set differently from the device IP address.
10. Click “OK”.
<Image Descriptions >
①
②
③
④
⑤

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⑥
⑦
⑧
⑨

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Control panel guide
①APP version : Application version of the software
②Start : Device connection
③Status (Condition or Status)
: Preview : Scanning is underway
④Frame Resolution
✓Normal : 1296 x 970 pixel resolution. Used for general time-lapse imaging.
✓High : 2592 x 1942 pixel resolution. Used for a single capture. Able to get a clearer image than
Normal resolution.
Caution! : To obtain the confluency data, you must capture or scan images using Normal. High does not perform
analysis; it is only possible to analysis an image that is Normal resolution.
⑤
④
⑭
②
Lamp : Meaning that currently lamp is brightfield.
Objective Lens : Meaning that lens magnification is 4x.
③
⑧
⑨
④
⑩
⑪
⑥
⑦
⑫
⑭
⑬
②
⑤
⑯
⑮
①
③
○
17 ⑱

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⑥Image information
✓Pixel Info(X,Y) : It shows the pixel intensity at the mouse cursor location (X,Y) on display.
Intensity ranges from 0 to 255.
1) Int : The value of intensity at the mouse cursor location on display. Higher intensity means
bright and lower intensity means dark.
2) Avg : Average of all intensity values on display
✓Image Size : Total number of pixels
✓Zoom : present magnification
⑦Scale bar : A visual indication of distance and feature size (page 19)
⑧Menu bar
✓Setup mode: Position, focus, vessel type, Image format, autofocusing setting can be adjusted before scanning.
✓Scan mode : It is a mode to set schedule for time-lapse imaging, showing the progress of time-lapse.
✓Album mode : Mode to show the files taken with Celloger Mini and video can be made.
✓Analysis mode : It calculates the confluence of images taken by Celloger Mini and growth curves can be drawn
for time-lapse images.
⑨X-Y-Z position : It shows the current coordinates of X, Y, Z
⑩Imaging control panel
⑪AutoFocus : Execute autofocusing depending on the autofocus settings in streaming status (refer to page 18 for
autofocus setting).
⑫
⑬Preview
✓Start Streaming : Preview on
✓Stop Streaming : Preview off
⑭Temperature & Humidity : Thermometer/hygrometer next to the LED light source in the bright field.
Note: The temperature and humidity sensors of Celloger Mini are not calibrated and used only for the purpose of
checking changes in value. If there is thermometer/hygrometer and calibration is required, contact Curiosis (via e-
mail) to request calibration.
⑮Blink me! : Upon pressing Blink me!, the LED indicator lights in white. Useful upon using multiple devices.
Lamp power : LED intensity
Brightness : Brightness of a camera
Contrast : Contrast between bright part and dark part
Measure : Measure the length of the desired part (page 18)
Select Layer : Composed of “BR”(brightfield image), “Cell”(confluency mark)(page 18)
Reset Zoom & Pan : Go back to Zoom and Pan state before adjustment
Temperature
Humidity

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Exchange : Function to make the stage come out of the device for easy mounting of vessel or vessel adapter.
⑯
✓Connection status: Showing device connection status
✓Device IP address : IP address of connected device
✓Device version : Firmware version of connected device
○
17 Display: It shows live or captured images.
⑱Mode setting panel: Section changes depending on mode.
Device
IP address
Device
version
Connection
status

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Basic operation
1. Device connection
1. Run CellogerMiniApp.
2. After a window pops up, select the icon of the device to be connected in Connect Device and click OK and then the
device is connected.
Note : If the device icon is not shown on the window, please check the followings.
①Check the PC IP address setting. (page 8)
②In case of multiple devices, refer to APPx A. (page 30)
③When Adapter is connected, select Network Adapter and select connected adapter by pressing an arrow.
①
②
①
②

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2. Cell monitoring [Positioning (position selection) and light intensity control]
1. Put the device in the incubator.
2. When the stage comes out by pressing , install the vessel. (In case of dishes or flasks, install the vessel
adapter first, then the vessel. Refer to Appx B for how to insert a vessel or vessel adapter (page 32). Upon installing
well plate, make sure that A1 is on the left front.
3. Select the vessel type in Setup mode. Well plate, dish, flask can be selected. Upon selecting vessel type, the vessel
type is displayed on the plate panel at the bottom right.
Note : A vessel adapter suitable for vessel type must be used. (use vessel adapter for vessels except for well plate)
4. Click and move desired vessel location on the plate panel with the mouse. (Upon clicking vessel or well in Plate
panel, it moves to center of vessel or well).
5. Upon clicking Start Streaming, a real-time image is shown on the display. [If the screen is black, light intensity
should be adjusted first (step 8)].
Plate panel
A1

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6. Use Jog or Manual position to adjust the position precisely. Rough adjustment is possible with Plate panel (page 17)
①Jog
: Move to the center of the stage.
: Move left and right. Using arrow button on the keyboard to move step by step.
: Move up and down. Using arrow button on the keyboard to move step by step.
: Use the scroll bar to move left and right for rough adjustment.
: Use the scroll bar to move up and down for rough adjustment.
: Stop the movement of stage
* It is possible to adjust motor speed and step distance.
Speed control: On the left circular slider, move the green arrow to change the value at the center of the circle
(can select the value from 10 to 100%)
Step control: On the right circular slider, move the blue arrow to change the value at the center and adjust the
unit after opening combo box (alternative keyboard “ * ”, “ / ”) (1 um ~ 10 mm)
Note : If the speed is set at value exceeding 30%, there is a risk of device malfunction so setting the value less
than 30% is recommended.
②Manual Position
After inputting the desired location in X, Y, Z
and press Go, the location is changed.
①
②

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7. Adjust focus manually or using AutoFocus . Autofocus works depending on the AF set range so perform this
job after adjusting AF with Jog (page 17).
8. On the Imaging control panel, adjust LED intensity, brightness and contrast by using scroll bar.
✓Lamp power :Adjustable from 0 to 255
✓Brightness : Adjustable from -2048 to 2047
✓Contrast : Adjustable from 0 to 160
Note : The recommended values are as follows. Lamp power: 135, Brightness: 256, Contrast: 40. The higher the
difference between cell and background, the better calculation of confluency (page 33).
9. Select Image Format and Frame Resolution in Imaging. Frame Resolution can also be selected on the left side of the
display.
10. To save the image, right-click on the display and click Save As. The saved images can be seen in ScreenCaptured file.
Two image files, one with raw image and the other one with feature are generated.
<Raw image>
< Image with feature >

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3. Details
Focusing
➢Manual adjustment
Adjust focus in Jog by using . Possible to adjust focus by using “ + ”, “ - ” on the number pad of the keyboard.
➢Auto focusing
The Celloger’s Autofocus algorithm scans within a certain depth and selects the area where cells are clearly shown.
Therefore, location should be set manually first and execute Autofocusing function.
1. Set the rough focus manually.
2. Adjust focus and press AutoFocus.
Note : In the AF panel in Setup mode, autofocusing can be set.
✓Depth [mm] : Range of AutoFocus fuction
✓Resolution [mm] : Scanning unit within depth
✓ROI Section : The area can be selected and AutoFocus
algorithm is executed based on the selected area.
Plate panel
Possible to set scanning order by right clicking the mouse on Plate panel. The table on the left side of the Plate panel
shows Scan Position Set.
✓Move to center position : Move to center position : Upon
clicking vessel on plate panel, it moves to the center.
✓Move to mouse position : Upon clicking the desired
location in the vessel on Ctrl + plate panel, it moves
to the location clicked.

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✓Is show ‘No’ : If it is checked, scanning order is displayed
on the plate panel and if it is not selected, the number
disappears.
✓Is snap to well : After selecting this, it is possible to move
from one well to another well using arrow keys on the
keyboard.
✓Scan direction : It shows the scanning order if all wells are
selected on the well plate. One Way scans in the
direction and Two Way scans in the direction.
✓Set all centers : Set position is possible for centers of all
wells on the well plate.
✓No : Scanning sequence
✓X, Y, Z : Each coordinate
✓Label : Well number
✓AF : Display whether autofocusing is run on Time-lapse imaging or not.
✓ : Able to delete all scan positions designated To delete just one
scan position, select the location to be deleted and press Delete key on the
keyboard.
✓ : Import the exported scan position table.
✓ : Save scan position table.
Features on display
Display features of the Display screen.
➢Measure, confluency mark (using icon located at the right bottom of Display)
①Measure
Select one of diagonal, horizontal and vertical directions, obtain data by
clicking one end to the other end of the area to be measured.
②BR : Streaming image or captured image layer
Cell : It is the layer that shows the surface covered by adherent cell in colors
when ‘run’ is pressed in the analysis mode. (refer to confluency analysis
and color setting page 23)
①
BR
Cell
②

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➢Zoom, Pan, Capture, Scale bar, Histogram, Center mark, Video area Grid line
(Using right-click of the mouse on Display)
✓Reset Zoom & Pan : Reset after using Zoom, Pan functions
✓Zoom : Click Ctrl + Mouse wheel to magnify images
✓Pan : Click Shift + Mouse Drag to move images
✓Save As… : Save images (raw image and captured image with features are
saved.)
✓Stage Position : Show current position on the right top of Display
✓Scale Bar : Show scale bar on the left bottom of Display
✓Histogram : Display Intensity Histogram of images
✓Center Mark : Display the reference point at the center of display in cross
mark
✓Video Area : Area shown in the video upon making video files.
✓Grid Lines : Draw grid line on Display and possible to change the number
of grid lines.

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Time-lapse imaging
1. Scan position setup (Setup mode)
1. Place the device into an incubator.
2. Mount vessel when the stages comes to the front after pressing . (Install vessel after installing vessel
adapter first in case of dish or flask.)
3. Select Vessel type and desired location on the plate panel.
4. Press Start Streaming.
5. Move to the desired location in the Setup mode. (page 15 step 6)
6. Adjust focus with Jog or Autofocus. (page 17)
7. Adjust brightness, contrast or lamp power, if necessary. (page 16 step 8)
8. Select Image Format and Frame Resolution on Imaging.
9. Designate the position by pressing on desired location. After designation, designated location is shown
on the plate panel and scan position set (page 17 for more details about Plate panel and Scan position set).
Note : Note : If Auto Focus is checked in and Set Position is executed, autofocusing function is
executed automatically. Align Image is location correction function. It corrects location if the location is stepped
out compared with the previous cycle during scanning. However, if interval time is 6 hours or longer or changes in
morphology are extreme within a short period of time, differences in image from previous cycle becomes large,
having a negative impact so in this case, it is better to turn off the setting.
2. Scanning (Scan mode)
Designation of file storage location and project name
In Scan mode, set the location (location where files will be saved) and project name. In the Location file, folder is
created in the name of project name and date set. If location designation is skipped, the file is saved in the scan file
and project name is not designated, folder name is created with the start time. Folder is created for each Set
Position.