cytiva F9-R User manual

Install
Fraction collector F9-R
Instructions
Scope
Instructions on how to install one, or for ÄKTA™ pure two, optional Fraction collector
F9-R (29011362) modules.
Description
Introduction
Fraction collector F9-R collects fractions from ÄKTA avant and ÄKTA pure purification
runs.
Fraction collector F9-R can be used for:
•Time or volume based fractionation
•Peak fractionation
•Combined time or volume based fractionation and peak fractionation
It is possible to install up to two Fraction collector F9-R modules when using ÄKTA pure.
ÄKTA avant has a built-in fraction collector, and it is possible to install one Fraction
collector F9-R as an extra module.
Note: If two fraction collectors are to be used, the second one must be configured as
Fraction collector F9-R 2nd, independent of the first fraction collector type.
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Location
ÄKTA pure
The illustration below shows Fraction collector F9-R together with ÄKTA pure. It is
recommended to place Fraction collector F9-R on the left side of the instrument.
Fraction collector F9-R can also be placed on a shelf located beneath the system.
ÄKTA avant
In ÄKTA avant it is recommended to place Fraction collector F9-R on the right side of the
instrument.
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Note: Consider the tubing going to Fraction collector F9-R if ÄKTA avant is turned
on its swivel foot.
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Illustration of Fraction collector F9-R
1
34
5
7
6
8
2
Part Function
1 Lock knob
2 Stationary part of delivery arm
3 Delivery arm
4 Tubing connector
5 Tube sensor
6 Collection tubes
7 Tube rack
8 Base unit
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Unpacking
Follow the instruction below to unpack the Fraction collector and to remove the
transport fixations.
Step Action
1Open the transportation box.
2Lift off the piece of foam and check the content according to the supplied
package list.
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Step Action
3Lift out Fraction collector F9-R by the Base unit and place it on the bench.
NOTICE
Never lift Fraction collector F9-R by the Delivery arm.
This may damage the Fraction collector.
4Remove the plastic bag.
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Step Action
5Lift the Delivery arm and gently move the Delivery arm to the outer stop.
6Remove the piece of foam from under the Tube rack.
Installation
For information on how to assemble Fraction collector F9-R, please refer to the
respective instrument User manual and Fraction collector F9-C and F9-R Operating
instructions.
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Illustration of Fraction collector F9-R
connector panel
12 2
Part Function
1 Node ID switch
2UniNet-9 communication and power supply
Connect the Fraction collector
Connect the UniNet cable between any of the UniNet-9 ports on the back of the
instrument and on the back of the Fraction collector F9-R or Fraction collector F9-R, 2nd.
Make sure that all unused UniNet-9 ports on the instrument are plugged.
Node ID
Node ID is a unit number designation that is used by the instrument to distinguish
between several units of the same type. All standard valves and available optional
modules are pre-configured to give the desired function. However, the function of a valve
or module can be changed by changing the Node ID.
Also, in a troubleshooting situation it may be useful to check the Node ID of the
respective valve or module.
Note: The function of a valve or module is defined by a combination of the module
type and its Node ID, not by its physical position.
Check/change Node ID
The Node ID is set by positioning the arrow of the rotating switch at the back of the
Fraction collector. Use a screwdriver to set the arrow of the switch to the desired
number.
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Step Action
1Set Node ID to 0 for Fraction collector F9-R and to 1 for Fraction collector F9-
R, 2nd.
2Connect Fraction collector F9-R and/or Fraction collector F9-R, 2nd to the
instrument.
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Software configuration
When a Fraction collector has been installed, the System Properties for the system
must be updated in UNICORN™. The system will restart automatically when the
configuration has been changed and the system can be reconnected.
Follow the instructions below to update the system in UNICORN.
Step Action
1•On the Tools menu in the Administration module, click System
Properties or click the System Properties icon to open the dialog.
Result:
The System Properties dialog is displayed.
•Select the system of interest in the System Properties dialog.
•Click Edit.
Note:
Only active systems can be edited.
Result:
The Edit dialog is displayed.
The ÄKTA pure dialog is shown in the example below.
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Step Action
2Select Fraction collectors from the Component types list.
Result:
All available fraction collectors are shown in the Component selection list.
•Click the checkbox to select the added fraction collector.
Note:
Instrument modules are referred to as Components in UNICORN.
3Click OK to apply the changes.
Check/Set delay volume
When a module has been installed after the UV monitor in the flow path, the delay
volume has to be adjusted in the System Settings dialog in UNICORN, to make sure that
the collected fractions correspond to the fractions indicated in the chromatogram.
Delay volumes can be set for the options Monitor to outlet valve, Monitor to frac,
Monitor to frac 2, and pH valve. Depending on the system configuration used, different
delay volume options will be available for selection in the System Settings dialog. The
delay volume has to be set for all displayed options.
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Delay volumes for ÄKTA avant and ÄKTA pure modules and standard tubing
configurations are found in the respective User Manual.
Follow the instructions below to check/set the delay volumes:
Step Action
1On the System menu in the System Control module, click Connect to
Systems or click the Connect to Systems icon.
Result:
The Connect to Systems dialog opens.
2•Select a system.
•Select Control mode.
•Click OK.
Result:
The selected instrument can now be controlled by the software.
3On the System menu, click Settings when the system is in state Ready.
Result:
The System Settings dialog is displayed. The ÄKTA pure dialog is shown in
the example below.
4•Select Tubing and Delay Volumes and select the delay volume option of
interest.
Note:
For ÄKTA avant Delay Volumes are found under Fraction collection.
•Check the delay volume in the Volume fields and enter a new value if
necessary.
•Click OK.
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Connect tubing
Follow the instructions in the table below to connect the tubing from ÄKTA avant and
ÄKTA pure to Fraction collector F9-R, or to both Fraction collector F9-R and Fraction
collector F9-R, 2nd.
Step Action
1Lift out the Tubing holder (4) from the Delivery arm (1).
3
4
5
6
Part Function
1 Delivery arm
2 Tube adjustment cavity
3 Sensor control
4 Tubing holder
5 Tubing holder nut
6 Exposed tubing end
2Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut (5)
from the Tubing holder.
3Insert the tubing through the Tubing holder.
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Step Action
4Place the Tubing holder with the tubing over the Tube adjustment cavity (2) of
the Delivery arm. Push the tubing down against the bottom of the Tube
adjustment cavity, and then fingertighten the Tubing holder nut. This ensures
the correct length of the exposed tubing end (6).
5Re-install the tubing holder in the Delivery arm.
6Check that the Tube sensor is in the correct position for the tube size. The
eluent tubing should be above the center of the collection tube. Use the
sensor control to position the Tube holder.
7Connection to ÄKTA pure
For Fraction collector F9-R: Connect the tubing from the Fraction collector
to:
•port Frac on Outlet valve V9-O, V9H-O, V9-Os or V9H-Os.
For Fraction collector F9-R, 2nd: Connect the tubing from the second
Fraction collector to:
•port Out 10 on Outlet valve V9-O or V9H-O.
•port Out 1 on Outlet valve V9-Os.
Connection to ÄKTA avant
For Fraction collector F9-R, 2nd: Connect the tubing from the second
Fraction collector to:
•port Out 10 on Outlet valve V9-O or V9H-O.
8Set the delay volume according to the length of the tubing used, according to
the instruction above.
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Standard tubing dimensions
The table below shows recommended standard tubing dimensions to connect Fraction
collector F9-R.
System Tubing
length (mm)
Tubing diameter (mm)
ÄKTA pure 25 400 0.50
ÄKTA pure 150 400 0.75
ÄKTA avant 25 500 0.50
ÄKTA avant 150 500 1.00
Note: Precut standard tubing for ÄKTA pure are delivered with the fraction
collector. For ÄKTA avant, cut the tubing according to the table above from
the spare tubing delivered with the ÄKTA avant system.
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cytiva.com/akta
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate.
ÄKTA and UNICORN are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.
All other third-party trademarks are the property of their respective owners.
© 2020–2021 Cytiva
UNICORN©2020–2021
Any use of UNICORN is subject to Cytiva Standard Software End-User License Agreement for Life Sciences Software Products. A
copy of this Standard Software End-User License Agreement is available on request.
All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva
business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most
current information.
For local office contact information, visit cytiva.com/contact
29021462 AD V:4 01/2021