cytiva UV-900 User manual

Monitor UV-900
Operating Instructions
Original instructions
Translation disc included
cytiva.com

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Table of Contents
1 Introduction ........................................................................................................ 5
1.1 About this manual ......................................................................................................................................... 6
1.2 Important user information ....................................................................................................................... 7
1.3 User documentation ..................................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 16
2.3 Emergency procedures ............................................................................................................................... 17
3 System description ............................................................................................ 18
3.1 Illustrations ...................................................................................................................................................... 19
3.2 Flow cell ............................................................................................................................................................. 21
3.3 Monitor principle ............................................................................................................................................ 22
4 Installation .......................................................................................................... 24
4.1 Unpacking ......................................................................................................................................................... 25
4.2 Site requirements .......................................................................................................................................... 26
4.3 Transport .......................................................................................................................................................... 27
4.4 Assembly ........................................................................................................................................................... 28
4.5 Connecting electrical cables ..................................................................................................................... 35
5 Operation ............................................................................................................ 37
5.1 Starting the instrument ............................................................................................................................... 38
5.2 Reading absorbance values ....................................................................................................................... 39
5.3 Menu selection and settings ..................................................................................................................... 40
5.3.1 Moving between menus ............................................................................................................................ 41
5.3.2 Return to main menu .................................................................................................................................. 43
5.3.3 Select value ..................................................................................................................................................... 44
5.3.4 Main menu overview ................................................................................................................................... 45
5.3.5 Setting lamp on and off .............................................................................................................................. 46
5.3.6 Setting wavelength ...................................................................................................................................... 47
5.3.7 Autozero ........................................................................................................................................................... 48
5.3.8 Setting analog output to an external chart recorder .................................................................... 49
5.3.9 Event mark ...................................................................................................................................................... 51
5.3.10 Noise filtering ................................................................................................................................................. 52
5.3.11 Check menu .................................................................................................................................................... 53
5.3.12 Setup menu ..................................................................................................................................................... 57
5.3.13 Setting and using the alarm timer ........................................................................................................ 59
5.3.14 Service displays ............................................................................................................................................. 61
5.4 Changing flow cell .......................................................................................................................................... 62
5.5 UV cell calibration .......................................................................................................................................... 63
5.6 Restart after power failure ......................................................................................................................... 68
6 Maintenance ....................................................................................................... 69
6.1 Cleaning before planned service ............................................................................................................. 70
6.2 Cleaning the instrument housing ............................................................................................................ 71
Table of Contents
Monitor UV-900 Operating Instructions 28962214 AF 3

6.3 User maintenance schedule ...................................................................................................................... 72
6.4 Cleaning-in-place .......................................................................................................................................... 73
6.5 Checking the instrument ............................................................................................................................ 74
6.6 Cleaning the flow cell and optical connectors .................................................................................... 76
6.7 Storage .............................................................................................................................................................. 78
7 Troubleshooting ................................................................................................. 79
7.1 General .............................................................................................................................................................. 81
7.2 Faults and actions .......................................................................................................................................... 82
7.3 Error messages ............................................................................................................................................... 84
8 Reference information ....................................................................................... 85
8.1 Menu overview ................................................................................................................................................ 86
8.2 Technical specifications .............................................................................................................................. 87
8.3 Procedures for decommissioning ........................................................................................................... 90
8.4 Regulatory information ............................................................................................................................... 91
8.4.1 Contact information .................................................................................................................................... 92
8.4.2 European Union and European Economic Area .............................................................................. 93
8.4.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 94
8.4.4 Regulations for North America ............................................................................................................... 96
8.4.5 Regulatory statements .............................................................................................................................. 97
8.4.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 98
8.5 Health and Safety Declaration Form ...................................................................................................... 100
8.6 Accessories and spare parts ...................................................................................................................... 102
8.7 Ordering information ................................................................................................................................... 104
Table of Contents
4 Monitor UV-900 Operating Instructions 28962214 AF

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices,
intended use of the Monitor UV-900 system, and lists of associated documentation.
In this chapter
Section See page
1.1 About this manual 6
1.2 Important user information 7
1.3 User documentation 9
1 Introduction
Monitor UV-900 Operating Instructions 28962214 AF 5

1.1 About this manual
Purpose of the Operating
Instructions
The Operating Instructions provides you with the instructions needed to handle
Monitor UV-900 in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
6 Monitor UV-900 Operating Instructions 28962214 AF

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use
Monitor UV-900 is a UV-absorption monitor for use in liquid chromatograhy.
Monitor UV-900 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Monitor UV-900 in the way it is intended:
•The user must have a general understanding of bioprocessing equipment and with
the handling of biological materials.
•The user should understand the concepts of liquid chromatography.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•Monitor UV-900 must be installed in accordance with the site requirements and
instructions in the Operating Instructions.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
1 Introduction
1.2 Important user information
Monitor UV-900 Operating Instructions 28962214 AF 7

WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
8 Monitor UV-900 Operating Instructions 28962214 AF

1.3 User documentation
In addition to these Operating Instructions, the documentation package supplied with
Monitor UV-900 also includes product documentation binders containing detailed
specifications and traceability documents.
The most important documents in the document package with regard to technical
aspects of Monitor UV-900 are listed below.
System-specific documentation
User documentation Content
Monitor UV-900 Operating Instructions Instructions needed to prepare and
operate the Monitor UV-900 system in a
correct and safe way.
EU Declaration of Conformity for
Monitor UV-900
Document whereby the manufacturer
ensures that the product satisfies and is
in conformity with the essential require-
ments of the applicable directives.
1 Introduction
1.3 User documentation
Monitor UV-900 Operating Instructions 28962214 AF 9

2 Safety instructions
About this chapter
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures and power failure of Monitor UV-900.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 16
2.3 Emergency procedures 17
2 Safety instructions
10 Monitor UV-900 Operating Instructions 28962214 AF

2.1 Safety precautions
Introduction
The Monitor UV-900 is powered by mains voltage and handles pressurized liquids that
may be hazardous. Before installing, operating or maintaining the monitor, you must
be aware of the hazards described in this manual. Follow the instructions provided to
avoid personal injury or damage to the equipment. The safety precautions in this
section are grouped into the following categories:
•General precautions
•Using flammable liquids
•Personal protection
•Installing and moving the instrument
•System operation
•Maintenance
General precautions
Always follow these General precautions to avoid injury when using the Monitor
UV-900 instrument.
WARNING
Do not operate Monitor UV-900 in any other way than described in
this manual.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not use any accessories not supplied or recommended by
Cytiva.
2 Safety instructions
2.1 Safety precautions
Monitor UV-900 Operating Instructions 28962214 AF 11

WARNING
Do not use Monitor UV-900 if it is not working properly, or if it has
suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
Using flammable liquids
When using flammable liquids with the Monitor UV-900, follow these precautions to
avoid any risk of fire or explosion.
WARNING
Fire Hazard. Before starting the system, make sure that there is no
leakage.
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of
Monitor UV-900.
2 Safety instructions
2.1 Safety precautions
12 Monitor UV-900 Operating Instructions 28962214 AF

WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
Installing and moving the instrument
WARNING
Supply voltage. Before connecting the power cord, make sure
that the supply voltage at the wall outlet corresponds to the
marking on the instrument.
WARNING
Protective ground. The product must always be connected to a
grounded power outlet.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
WARNING
High intensity UV light. This product uses high intensity ultra-
violet light. Do not disconnect the optical fibers while the lamp is
on.
2 Safety instructions
2.1 Safety precautions
Monitor UV-900 Operating Instructions 28962214 AF 13

CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
System operation
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be
completely dry on the inside and the outside before reconnecting
the power supply.
WARNING
Hazardous biological agents during run. When using
hazardous biological agents, run System CIP and Column CIP to
flush the entire pump with bacteriostatic solution (e.g. 1M NaOH)
followed by a neutral buffer and finally distilled water, before
service and maintenance.
WARNING
Hazardous chemicals during run. When using hazardous chemi-
cals, run System CIP and Column CIP to flush the entire system
tubing with distilled water, before service and maintenance.
Maintenance
WARNING
Electrical shock hazard. All repairs should be done by service
personnel authorized by Cytiva. Do not open any covers or replace
parts unless specifically stated in the user documentation.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
2 Safety instructions
2.1 Safety precautions
14 Monitor UV-900 Operating Instructions 28962214 AF

WARNING
Corrosive substance. NaOH is corrosive and therefore dangerous
to health. When using hazardous chemicals, avoid spillage and
wear protective glasses and other suitable Personal Protective
Equipment (PPE).
WARNING
Hazardous chemicals in UV flow cell. Make sure that the entire
flow cell has been flushed thoroughly with bacteriostatic solution,
for example NaOH, and distilled water, before service and mainte-
nance.
WARNING
Hazardous chemicals or biological agents. When using
hazardous chemicals or biological agents, make sure that the
entire system has been flushed thoroughly with bacteriostatic
solution (e.g., NaOH) and distilled water before service and mainte-
nance.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before replacing any component on the instrument, unless
stated otherwise in the user documentation.
2 Safety instructions
2.1 Safety precautions
Monitor UV-900 Operating Instructions 28962214 AF 15

2.2 Labels
Introduction
This section describes the system label on Monitor UV-900 and its meaning.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
The following symbols and text may be present on the system label:
Label text Description
Code no: Code number.
Serial no: Serial number.
Mfg Year: Year (YYYY) and month (MM) of manufacture.
Voltage: Voltage.
Frequency: Supply voltage frequency.
Max power: Max power.
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
2 Safety instructions
2.2 Labels
16 Monitor UV-900 Operating Instructions 28962214 AF

2.3 Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of Monitor UV-900.
The section also describes the result in the event of power failure.
Emergency shutdown
In an emergency situation, do as follows to stop the run:
Switch off power to the instrument by switching the On/off switch on the rear panel to
the (O) position. If required, disconnect the mains power cord. The run is interrupted
immediately.
Power failure
In the event of power failure the run is interrupted immediately.
2 Safety instructions
2.3 Emergency procedures
Monitor UV-900 Operating Instructions 28962214 AF 17

3 System description
Monitor UV-900 is a UV-absorption monitor for use in liquid chromatography. Monitor
UV-900 features:
•Variable wavelength for detection in the range 190–700 nm.
•Up to 3 wavelengths can be monitored simultaneously.
•Two alternative flow cells with path length 2 mm and 10 mm.
•Accurate and reliable monitoring through self-test, self-calibration and fiber optics.
In this chapter
Section See page
3.1 Illustrations 19
3.2 Flow cell 21
3.3 Monitor principle 22
3 System description
18 Monitor UV-900 Operating Instructions 28962214 AF

3.1 Illustrations
Main unit
Monitor U V-9 00
1 2
3
4
Part Function
1 Display
2 Menu selection dial
3 Cell holder
4 Flow cell
3 System description
3.1 Illustrations
Monitor UV-900 Operating Instructions 28962214 AF 19

Main unit, rear panel
Analogue out
0-1 V
Mains On/o
Remote Uninet 1
12 3 4 5
Part Function
1Analogue output 0-1 V. Recorder output, 3 channels 0 to 1 V
2Remote. Digital signal inputs, lamp on/off, autozero, event mark.
3Uninet 1. Computer network.
4Mains power inlet. Supply voltage, grounded.
5On/off. Instrument power switch.
3 System description
3.1 Illustrations
20 Monitor UV-900 Operating Instructions 28962214 AF