DMT 360CW User manual

USER MANUAL, VOL.2.0
WIRE MYOGRAPH SYSTEM - 360CW & 320A

User Manual
Version 1.0
WIRE MYOGRAPH SYSTEMS
360CW AND 320A
USER MANUAL

3WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL
TRADEMARKS
PowerLab® and LabChart® are registered trademarks of ADInstruments Pty. Ltd. The names of specic recording units,
such as PowerLab 4/35, are trademarks of ADInstruments Pty. Ltd.
DMT reserves the right to alter specications as required.
This document was, as far as possible, accurate at the time of printing. Changes may have been made to the software and
hardware it describes since then. New information may be supplied separately.
This documentation is provided with the Wire Myograph system
No part of this document may be reproduced by any means without the prior written permission of DMT A/S.
Copyright © Danish Myo Technology A/S

4
CONTENTS
CONTENTS
Trademarks .......................................................................................................................................................................................................................................2
Safety ..................................................................................................................................................................................................................................................4
EC Declaration of Conformity ....................................................................................................................................................................................................4
Chapter 1 - System overview .....................................................................................................................................................................................................6
1.1 Wire Interface - front and rear.............................................................................................................................................................................................6
1.2 Wire Myographs.......................................................................................................................................................................................................................7
1.2.1 Confocal Wire Myograph - 360CW.............................................................................................................................................................................7
1.2.2 Single Wire Myograph - 320A......................................................................................................................................................................................8
Chapter 2 - Setting up ..................................................................................................................................................................................................................9
2.1 Setting up the complete Wire Myograph System .......................................................................................................................................................9
2.2 The rst force calibration................................................................................................................................................................................................... 10
Chapter 3 - The wire Interface Menus.................................................................................................................................................................................. 11
3.1 General description on how to navigate the touch screen................................................................................................................................... 11
3.2 Power-up screen................................................................................................................................................................................................................... 11
3.3 Main Menu.............................................................................................................................................................................................................................. 12
3.4 Zero Menu............................................................................................................................................................................................................................... 12
3.5 Heat Menu .............................................................................................................................................................................................................................. 13
3.6 Timer and Buzzer Menu ..................................................................................................................................................................................................... 13
3.7 Settings Menu ....................................................................................................................................................................................................................... 14
3.7.1 Force Calibration Menu............................................................................................................................................................................................... 14
3.7.2 pH Calibration Procedure........................................................................................................................................................................................... 18
3.7.3 Select Analog Output (optional) ............................................................................................................................................................................ 19
3.7.4 Interface Settings.......................................................................................................................................................................................................... 20
3.7.4.1 Temperature Dierence (oset)........................................................................................................................................................................ 20
3.7.4.2 pH Set-up Menu..................................................................................................................................................................................................... 20
3.7.4.3 Factory Diagnostics............................................................................................................................................................................................... 20
Appendix 1 - System specications...................................................................................................................................................................................... 21
Notes................................................................................................................................................................................................................................................ 23

5WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL
SAFETY
The Wire Myograph System has been designed for use only in teaching and research applications. It is not intended for clinical
or critical life-care use and should never be used for these purposes. Nor for the prevention, diagnosis, curing, treatment, or
alleviation of disease, injury, or handicap.
• Do not open the apparatus: the internal electronics pose a risk of electric shock.
• Do not use this apparatus near water.
• To reduce the risk of re or electric shock, do not expose this apparatus to rain or moisture. Objects lled with liquids should
not be placed on the apparatus.
• Do not block any ventilation openings. Install in accordance with the manufacturer’s instructions.
• Do not install near any heat sources such as radiators, heat registers, stoves, or other apparatus that produce heat.
• Only use attachments and accessories specied by the manufacturer.
• Unplug this apparatus during lightning storms or when unused for long periods of time.
The Wire Interface is delivered with an external 100-240VAC to 24VDC adapter. Protect the power adapter and cord from being
walked on or pinched. Particularly at power plugs and the point where they connect to the apparatus. Refer all servicing to
qualied service personnel. Servicing is required when the apparatus has been damaged in any way; such as, the power-supply
cord or plug is damaged, liquid has spilled onto or objects have fallen into the apparatus, the apparatus has been exposed to rain
or moisture, does not operate normally, or has been dropped.
EC DECLARATION OF CONFORMITY
Danish Myo Technology A/S
Certify and declare that the following apparatus:
Wire Myograph System - DMT360CW, DMT320A
Restrictive use: Only for laboratory use.
Manufactured by:
Danish Myo Technology A/S
Skejbyparken 152
8200 Aarhus N.
Denmark
Conforms with the essential requirements of the EMC Directive 2004/108/EC.
Based on the following specications applied by:
EN 61326-1:2006
EN 61326-2-6:2006
EN 61326-2-6/Corr.:2007
And with the LVD Directive 2006/95/EC.
Based on the following specications applied by:
EN 61010-1:2010
EN 61010-2-030:2010
General warnings regarding EMC:
Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g. unshielded intentional RF sources),
as these may interfere with the proper operation.

6UNPACKING THE WIRE MYOGRAPH SYSTEM
UNPACKING THE WIRE MYOGRAPH SYSTEM
Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during
handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued as
quickly as possible. If the packing material appears damaged, please retain it until a possible claim has been settled.
We recommend that you store the packing material for any possible future transport of the Wire Myograph System. Please
contact DMT Sales Department for packing instructions if the original packing material is unavailable.
After unpacking your new Wire Myograph System, please use the following list to check that the system is complete:
Wire Interface
• Power supply (the shape of the AC plug varies by country; be sure that the plug has the right shape for your location)
Confocal Wire Myograph - 360CW or Wire Myograph - 320A
• Myograph connection cable with a temperature probe
• Plastic mounting jaws (360CW) or stainless steel mounting jaws with supports (320A)
• Chamber cover
Accessories:
• 1 x calibration kit (including bridge, T-balance and 2 gram weight)
• 1 x funnel
• 1 x stainless steel wire 40 µm
• 2 x Allen keys (360CW) or 3 x Allen keys (320A)
• 1 x high vacuum grease
• 1 x grease for protection of linear slides
• 4 x spare screws (360CW) for mounting of jaws or 5 x spare screws (320A)
• 1 x screwdriver
• 10 x spare cover glass od 12,0 x 0,17 mm
Optional Accessories:
• PowerLab
• LabChart
• DMT Device Enabler
• Stimulator
• pH meter
• Vacuum Pump
• Electronic vacuum valve
• Waste bottle
• Gas supply manifold

7WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL
CHAPTER 1  SYSTEM OVERVIEW
1.1 Wire Interface - front and rear
Figure 1.2 Wire Interface - rear
ON/OFF switch
24V DC port
for power
Connection port for
Wire Myograph
Analogue recorder output - connection to
external data acquisition software (optional)
USB connection port
for computer Connection port
for pH meter
(optional)
Grounding, connect to
Data Acquisition
Figure 1.1 Wire Interface - front
Power indicator
Touch display

8CHAPTER 1
Window in the base of the
chamber for imaging
Figure 1.3 Confocal Wire Myograph - 360CW with close-up of the chamber
25-pin serial cable for communication
with Wire Interface
Allen screws for ne alignment
of the wire jaws
Wire jaw connected to
force transducer
Force transducer pin
Vertical micrometer screw
Wire jaw connected to
horizontal micrometer
Horizontal micrometer
1.2 Wire Myographs
1.2.1 Confocal Wire Myograph - 360CW

9WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL
1.2.2 Single Wire Myograph - 320A
Port for connection to Wire Interface
Wire jaw connected to force transducer
Wire jaw connected to
micrometer
Micrometer
Suction pipe for connection
to vacuum pump
Pipe for gas supply Pipe for lling the chamber
(using the 40 mm funnel)
Window at the bottom of the
chamber for imaging
(under the wire jaws)
Force transducer pin
Figure 1.1 Single Wire Myograph with close-up of chamber
Transducer house
Allen screws for ne alignment of the
wire jaws (2 on each side)

10
CHAPTER 2  SETTING UP
2.1 Setting up the complete Wire Myograph System
Figure 2.1 Example of a complete Wire Myograph System - 320A
CHAPTER 2
Figure 2.1 is an example of a complete set-up for the Wire Myograph System - 320A. A set-up for Confocal Wire Myograph would
look the same but with a Confocal Wire Myograph instead. The set-up includes optional equipment such as pH meter for measur-
ing pH in the buer, an electronic vacuum valve, suction bottle, vacuum pump, a computer and Data Acquisition System.
IMPORTANT
TO RECORD A NOISEFREE SIGNAL IT IS IMPORTANT TO CONNECT THE WIRE INTERFACE GROUND CONNECTION TO THE
DATA ACQUISITION SYSTEM’S GROUND CONNECTION.
1. Wire Myograph to Wire Interface connection:
Connect the Wire Myograph to the Wire Interface using the grey 44/25-pin connector cable. The end of the cable with the
temperature probe is attached to the Wire Myograph.
2. Wire Interface to computer connection:
Data acquisition is possible either by connecting the Wire Interface directly to a computer, or through a Data Acquisition
System able to collect voltage output (0-2.5volts) such as a PowerLab Data Acquisition System or similar system.
• Direct computer USB connection for Labchart (AD Instruments) users (latest version of Labchart):
Connect the Wire Interface to the computer with the USB cable from the back panel to the USB port.
• Analog Data Output through a PowerLab or similar Data Acquistion System (optional):
Connect the Wire Interface to the analog data acquisition system using BNC cables. Connect Rec 1 (Chamber 1) on the
Wire Interface to Input 1 on the analog data acquisition system. Rec 2 (Chamber 2) to Input 2 on the analog data acqui-
sition system 2, and so forth. Connect the analog data acquisition system to one of the USB-ports on the computer.
Gas supply manifold
Data Acquisition System
via BNC cables or USB
Wire Interface
rear panel
Wire Myograph
pH Meter
Oxygen/
Carbogen/
Compressed air
Vacuum Pump
Suction Bottle
Electronic vacuum valve
Power supply