DMT 560TP User manual

1TISSUE PULLER - MODEL 560TP - USER MANUAL
USER GUIDE

2
TISSUE PULLER  560TP
USER GUIDE

3TISSUE PULLER - MODEL 560TP - USER MANUAL
CONTENTS
Trademarks .......................................................................................................................................................................................................................................2
Safety ..................................................................................................................................................................................................................................................4
Unpacking the Tissue Puller System........................................................................................................................................................................................5
Chapter 1 - System overview .....................................................................................................................................................................................................6
1.1 Tissue Puller - 560TP front and rear panel......................................................................................................................................6
Chapter 2 - Setting up ..................................................................................................................................................................................................................7
2.1 The complete Tissue Puller System - 560TP ...................................................................................................................................7
2.2 Setting up step-by-step .....................................................................................................................................................................7
2.3 Conguration and calibration of the 560TP System.......................................................................................................................8
2.3.1 Conguration of the 560TP ....................................................................................................................................................9
2.3.2 Weight Calibration of Force transducer.................................................................................................................................9
Chapter 3 - The MyoPULL menus ......................................................................................................................................................................................... 11
3.1 MyoPULL Menu............................................................................................................................................................................... 11
3.2 View Menu ...................................................................................................................................................................................... 12
3.3 Experimental setup ........................................................................................................................................................................ 12
Chapter 4 - Transducer Checking and maintenance ...................................................................................................................................................... 14
4.1 Checking the force transducer ..................................................................................................................................................... 14
4.2 Force Transducer Replacement..................................................................................................................................................... 15
4.3 Maintenance................................................................................................................................................................................... 15
Appendix 1 - Reading a millimetre micrometer............................................................................................................................................................... 16
Appendix 2 - System specications...................................................................................................................................................................................... 17
Appendix 3 - EC Declaration of Conformity....................................................................................................................................................................... 18
Notes................................................................................................................................................................................................................................................ 19

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CHAPTER 1  SYSTEM OVERVIEW
1.1 Tissue Puller - 560TP front and rear panel
Figure 1.1 Tissue Puller - 560TP front panel
Figure 1.2 Tissue Puller - 560TP back rear panel
USB port
ON/OFF switch
Power inlet - 24VDC 0,75A (V+ on center pin)
Micrometer
Motor
Force transducer
Force transducer hook
Jaws
Power ON indicator

5TISSUE PULLER - MODEL 560TP - USER MANUAL
CHAPTER 2  CLEANING AND MAINTENANCE
The Tissue Puller is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections
are read carefully and that the instructions are followed at all times.
2.1 Cleaning the Tissue Puller
2.2 Maintenance of the force transducer
Force transducer: The force transducer is the most delicate and fragile component of the 560TP. Extreme care must be used
when handling or touching the force transducer. As part of daily maintenance, inspect the grease around the transducer pin ex-
tending from the transducer compartment pinhole before starting any experiment. Insufcient grease in this area will allow buffer
and water to enter the transducer compartment and cause irreparable damage to the force transducer.
IMPORTANT NOTES
DMT RECOMMENDS THAT THE HIGH VACUUM GREASE SEALING THE TRANSDUCER PINHOLE IS CHECKED AND REPLACED
AT LEAST ONCE A WEEK, ESPECIALLY IF THE MYOGRAPH IS USED FREQUENTLY
DMT TAKES NO RESPONSIBILITY FOR THE USE OF ANY OTHER KIND OF GREASE OTHER THAN THE GREASE SUPPLIED BY
DMT FOR THIS PURPOSE
DMT TAKES NO RESPONSIBILITY FOR ANY KIND OF DAMAGE CAUSED BY MISHANDLING OF THE FORCE TRANSDUCERS
2.2.1 Checking the force transducer
The 560TP force transducer is a strain gauge connected to a Wheatstone bridge. The force transducer is housed in a separate,
protective compartment (see gure 4.1 below). While the protective cover offers some mechanical protection for the force trans-
ducers, it is still very vulnerable to applied forces exceeding 1 Newton (100 grams) or uid running into the transducer compart-
ment due to inadequate greasing of the transducer pinhole (see chapter 4.3 Maintenance). If the force readings in MyoPULL ap-
pear unstable or noisy, then perform a new calibration of the force transducer as described in chapter 2.3.2 - Weight Calibration
of force transducer. During the calibration monitor the relative force reading values in the FORCE CALIBRATION sub-menu in the
MyoPULL Software. The normal operating values for the force transducer during calibration should be between 3000 and 3500.
• If the value is 0, a single digit, or a three digit number, the force transducer is broken and needs to be replaced.
• If the value is less than 2000 or greater than 4500, the force transducer is broken and needs to be replaced.
In addition, if the force reading cannot be reset to zero AND the transducer cannot be recalibrated, the force transducer is broken
and needs to be replaced. If any other problems related to the force transducer are encountered, please contact DMT for advice
or further instructions.
2.2.2 Force Transducer Replacement
If the force transducer breaks and needs to be replaced the 560TP need to be shipped back to DMT for replacement. The ship-
ping address is:
Danish Myo Technology A/S
Skejby Science Center
Skejbyparken 152
DK-8200 Aarhus N.
Denmark
Tel: [phone]
Please contact your Sales Representative and request a Return to Manufacturer Authorisation (RMA) Number to attach to your
shipping documentation.
CHAPTER 2

6APPENDIX 1
APPENDIX 1  READING A MILLIMETRE MICROMETER
Sleeve scale
The micrometer sleeve scale has a total length of 25 mm divided into fty equal parts. Each part of division above the horizontal
line represents 1 mm where each fth line is marked by a longer line and a number, which designates the length in mm. Each part
of division below the horizontal line is placed between each 1 mm mark (scale above the horizontal line) and represents 0.5 mm.
Thimble scale
The thimble is divided into fty equal parts, and one complete rotation of the thimble is indicated by the smallest division on the
sleeve, which equals 0.5 mm. Each divi¬son on the thimble scale is 10 μm. If the thimble scale falls between two lines, then a
number between 0 and 10 μm must be approximated.
Example 1
1. Note that the thimble has stopped at a point beyond “10” on the sleeve indicating 10000 µm (10 mm).
2. Note that there is no mark completely visible between the 10 mm mark and the thimble.
3. Read the value on the thimble corresponding to the intersection with the horizontal line on the sleeve.
A. Reading on sleeve:
B. No additional mark visible:
C. Thimble reading:
Total reading:
Example 2
1. Note that the thimble has stopped at a point beyond “16” on the sleeve indicating 16000 µm (16 mm).
2. Note that this time a mark is visible between the 16 mm mark and the thimble indication 500 µm.
3. Read the value on the thimble corresponding to the intersection with the horizontal line on the sleeve.
A. Reading on sleeve:
B. One additional mark visible:
C. Thimble reading:
Total reading:
Figure A1.1 Overview of the micrometer parts (actual reading 20000 µm = 20 mm)
Sleeve scale Thimble scale
Figure A1.2 Example 1:
reading = 10380 µm
10000 µm
0 µm
380 µm
10380 µm
Figure A1.3 Example 2:
reading = 16780 µm
16000 µm
500 µm
280 µm
16780 µm

7TISSUE PULLER - MODEL 560TP - USER MANUAL