DMT 820MS User manual

USER MANUAL, VOL.2.0
MUSCLE STRIP MYOGRAPH SYSTEM - 820MS

2MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
MUSCLE STRIP MYOGRAPH SYSTEM
MODEL 820MS

3
MUSCLE STRIP MYOGRAPH SYSTEM
MODEL 820MS
TRADEMARKS
PowerLab® and LabChart® are registered trademarks of ADInstruments Pty Ltd.
The names of specic recording units, such as PowerLab 4/35, are trademarks of ADInstruments Pty Ltd.
Pentium is a registered trademark of the Intel Corporation. Windows, Windows 95, Windows 98, Windows ME, Windows NT,
Windows 2000, Windows XP, Windows Vista and Windows 7 are registered trademarks of Microsoft Corporation.
All other trademarks are the properties of their respective owners.
DMT reserves the right to alter specications as required.
This document was, as far as possible, accurate at the time of printing.
Changes may have been made to the software and hardware it describes since then.
New information may be supplied separately.
This documentation is provided with the DMT Muscle Strip Myograph System – Model 820MS
Document Number: 820MS – UG2.2A
All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means
without the written permission of Danish Myo Technology A/S.
Every attempt is made to ensure accurate information, misprints, construction- and specication changes can occur.
Danish Myo Technology A/S reserves the right to alter/change content as required and without any notice.
Copyright © Danish Myo Technology A/S
Trademarks

4MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
CONTENTS
Trademarks .......................................................................................................................................................................................................................................3
Introduction .....................................................................................................................................................................................................................................5
Safety ..................................................................................................................................................................................................................................................6
EMC / EMI ..........................................................................................................................................................................................................................................7
Approvals ..........................................................................................................................................................................................................................................7
Certicate of Conformity.............................................................................................................................................................................................................8
About this manual .........................................................................................................................................................................................................................9
Unpacking the myograph system......................................................................................................................................................................................... 10
Chapter 1 - System overview .................................................................................................................................................................................................. 11
1.1 Myo-Interface front panel............................................................................................................................................................... 11
1.2 Interface Rear Panel ...................................................................................................................................................................... 11
1.3 Muscle Strip myograph unit........................................................................................................................................................... 12
Chapter 2 - Setting-up............................................................................................................................................................................................................... 13
2.1 The complete Muscle Strip Myograph System - 820MS.............................................................................................................. 13
2.2 Setting up step-by-step .................................................................................................................................................................. 13
2.3 The rst weight calibration............................................................................................................................................................. 14
Chapter 3 - The Interface Menus............................................................................................................................................................................................ 15
3.1 Interface Menus – Navigation ....................................................................................................................................................... 15
3.2 Interface Menus – Calibrated Mode ............................................................................................................................................. 16
3.3 Interface Menus – Direct Mode .................................................................................................................................................... 22
Chapter 4 - The Muscle Strip Myograph unit..................................................................................................................................................................... 24
4.1 Calibration of the force transducer ............................................................................................................................................... 24
4.2 Checking the force transducer ...................................................................................................................................................... 26
4.3 Force Transducer Replacement..................................................................................................................................................... 26
4.4 Myograph Maintenance ................................................................................................................................................................. 28
4.5 Cleaning the myograph .................................................................................................................................................................. 29
Chapter 5 - The mounting supports and mounting in the 820MS chamber......................................................................................................... 30
Appendix 1 - System specications...................................................................................................................................................................................... 31

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INTRODUCTION
The Muscle Strip Myograph System - 820MS represents the rst state-of-the-art 4-channel myograph system for muscle strips
of up to 15 mm in length. DMT developed this system for simultaneous measurement of up to 4 isolated muscle preparations,
mounted as strips, between clamp supports. Several types of mounting supports exist, depending on the user’s preferences.
Customized mounting supports can be made upon request. In addition, because of the somewhat rectangular chamber, the
myograph also can be used for the study of larger strips from other organs, including larger, longer strips of smooth muscle.
Stimulation electrodes are available (optional). The electrodes are built into the chamber cover and can be used to activate the
muscle via eld stimulation from a pulse/train stimulator, such as the DMT CS200.
The muscle bath unit is constructed of aluminum, while the centrally located rectangular chamber is stainless steel for easy
cleaning. The segment clamp supports are positioned in the chamber with one side attached to a force transducer and the other
side to a micro-manipulator. The micromanipulators permit the accurate setting of passive tension (which correlates with muscle
length) while the sensitive force transducers measure the developed isometric muscle tension during stimulation. Each unit has
individually controlled gas inow and suction. The system is automatically heated to a set temperature, up to 45° C.
Following mounting and equilibration, the optimal pre-load or passive tension for the muscle can be determined. The length of
the muscle is kept constant during the experiment; thereby any contractions measured from the isolated, mounted muscle strip
will be examined under isometric conditions. Compounds can be added directly to the chamber, and muscle tension can be
monitored to evaluate the chronotropic or inotropic effects of the compound. If desired, the buffer can be sampled for metabolic
factors during and after muscle contraction(s).
This myograph is well-suited for high-throughput analysis of muscle reactivity, especially when multiple units are arranged
conveniently side-by-side. The 820MS is ideal for drug development and testing related to striated muscle, as well as for
experiments where comparing multiple muscle samples from control and treated or gene-modied animals is required. The
multitude of data generated from the force transducers can be captured through external data acquisition via either calibrated
(ltered) or uncalibrated (unltered) analog outputs or in digital format via a serial connection.
INTRODUCTION

6MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
SAFETY
The Muscle Strip Myograph System - 820MS has been designed for use in teaching and research only. It is not intended for clinical
or critical life-care use and should never be used for these purposes. The 820MS also should not be used for the prevention,
diagnosis, curing, treatment, or alleviation of disease, injury, or handicap. The following are other safety concerns of note:
• Do not open the unit; the internal electronics pose a risk of electric shock.
• Do not use this apparatus near water.
• To reduce the risk of re or electric shock, do not expose this apparatus to rain or moisture. Objects lled with liquids should
not be placed on the system.
• Do not block any ventilation openings. Install in accordance with the manufacturer’s instructions.
• Do not install near any heat sources such as radiators, heat registers, stoves, or other items that produce heat.
• Only use attachments and accessories specied by the manufacturer.
• Unplug this system during lightning storms or when unused for long periods of time.
• Be advised that different operating voltages require the use of different types of line cord and attachment plugs. Check the
voltage in your area and use the correct type. See the table below:
Voltage Line plug according to standard
110–125 V UL81 and CSA C22.2 No. 42
220–230 V CEE 7 page VII, SR section 107-2-D1/IEC 83, page C4
240 V BS 1363 of 1984 Specication for 13A fused plugs and switched and
unswitched socket outlets.
Protect the power cord from being walked on or pinched: particularly at power plugs and the point where they connect to the
apparatus.
Refer all servicing to qualied service personnel. Servicing is required when the apparatus has been damaged in any way; such
as, the power-supply cord or plug is damaged, liquid has spilled onto or objects have fallen into the apparatus, the apparatus has
been exposed to rain or moisture, does not operate normally, or has been dropped.

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EMC / EMI
This equipment has been tested and found to comply with the limits for a Class B Digital device, pursuant to part 15 of the
FCC rules. These limits are designed to provide reasonable protection against harmful interference in residential installations.
This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the
instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not
occur in a particular installation. If this equipment does cause harmful interference to radio or television reception (which can
be determined by monitoring the interference while turning the equipment off and on), the user is encouraged to correct the
interference by one or more of the following measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and receiver.
• Connect the equipment into an outlet on a circuit different to that which the receiver is connected to.
• Consult the dealer or an experienced radio/TV technician for help.
APPROVALS
Complies with the EMC standards:
EMC 89/336/EEC: EN 61326-2-6:2005
EN 61000-3-2.
Certied with the safety standards:
Directive 2006/95/EC: EN 61010-1:2001
EN 61010-1/Corr.1:2003
EN 61010-1/Corr.1:2003
EN 61010-2-101:2003
EMC/EMI

8MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
CERTIFICATE OF CONFORMITY
DMT A/S, Skejbyparken 152, 8200 Aarhus N., Denmark,
hereby declares its responsibility that the following product:
Muscle Strip Myograph System
Model 820MS
is covered by this certicate and marked with CE-label conforms with the following standards:
EN 61010-1:2001
EN 61010-1/Corr.1:2003
EN 61010-1/Corr.1:2003
EN 61010-2-101:2003
EN 61326-2-6:2005
Safety requirements for electrical equipment for measurement, con-
trol, and laboratory use -
Part 1: General requirements.
Safety requirements for electrical equipment for measurement, con-
trol and laboratory use - Part 2-101: Particular requirements for in
vitro diagnostic (IVD) medical equipment.
Electrical equipment for measurement, control and laboratory use -
EMC requirements - Part 2-6: Particular requirements - In vitro diag-
nostic (IVD) medical equipment.
With reference to regulations in the following directives: 2006/95/
EC, 89/336/EEC

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ABOUT THIS MANUAL
This manual contains a complete list of procedures that describe how to install, maintain and use the Muscle Strip Myograph
System – 820MS.
Chapter 1 provides an overview of the construction and basic features of the Myo-Interface and the myograph unit.
Chapter 2 describes step-by-step how to set-up the complete Muscle Strip Myograph System – 820MS including accessories.
Chapter 3 is a complete manual to the Myo-Interface. The chapter describes in detail the construction of the menu system and
how to use all the features of the Muscle Strip Myograph System - 820MS.
Chapter 4 contains procedures describing general as well as daily maintenance of the myograph unit; e.g. adjustment of sup-
ports and cleaning instructions.
Appendix contain system specications.
ABOUT THIS MANUAL

10 MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
UNPACKING THE MYOGRAPH SYSTEM
Take a few minutes to carefully inspect your new Muscle Strip Myograph System - 820MS for damage which may have occurred
during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued
soon as possible. If the packing material appears damaged, please retain it until a possible claim has been settled.
We recommend that you store the packing material for any possible future transport of the Muscle Strip Myograph System. In
case of transport and the original packing material is unavailable, please contact DMT Sales Department for advice and packing
instructions.
After unpacking your new Myograph System, please use the following list to check that the system is complete:
• 1 interface unit
• 4 chamber units with mounted clamp supports
• 4 chamber covers
• 1 external temperature probe
• 1 power cord*
• 1 calibration kit (including bridge, balance and 2 g weight)
• 1 tube of high vacuum grease
• 1 tube of grease for screw thread
• 2 Allen keys
• 1 small screwdriver
• 1 CD with an user manual for Muscle Strip Myograph System
* The shape of the AC plug varies by country; be sure that the plug ts the outlets for your location.