Fisher Scientific Isotemp 642 User manual

Hybridization Incubators
Installation and Operation Manual
103751 Rev. 2
© 2010 Fisher Scientific Inc.
All rights reserved
Printed in U.S.A.
Isotemp

Fisher Scientific
MANUAL NUMBER 103751 (7000621)
2 40460 9/9/15 Removed CE references ccs
1 31325 2/13/15 Added explosion and fire cautions ccs
0 -- 5/13/10 Transfer to Marietta (was 103751 10/4/06) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Hybridization Incubators i
Manuals covered in this manual
Catalog Number Model Number Size Voltage
13-247-10Q 642 Small 120V
13-247-11Q 621 Small 100V
13-247-12Q 625 Small 230V
13-247-13Q 630 Small 230V UK
13-247-20Q 632 Medium 120V
13-247-21Q 634 Medium 100V
13-247-22Q 636 Medium 230V
13-247-30Q 638 Dual 120V
13-247-31Q 640 Dual 100V

Fisher Scientificii Hybridization Incubators
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Fisher Scientific makes no representations or warranties with respect to this manual. In
no event shall Fisher Scientific be held liable for any damages, direct or incidental, arising out of or related to the
use of this manual.
Fisher Scientific reserves the right to change specifications or designs at any time without incurring obligation.
Warning This unit is not explosion-proof. Do not use in the presence of flammable or combustible materials;
fire or explosion may result. Unit contains components that may ignite such materials. Before operating oven,
always observe the following safety precautions:
• Fumes and spillage from acidic solutions cause corrosion of the stainless steel chamber. Care should be
taken to maintain a neutral pH at all times.
• The heater for the oven is in the bottom of the unit. Surface temperatures at the bottom cover of the
unit may be higher than set point temperature. For example: A plastic container on the heater cover may
become hot enough to melt/burn the container at settings below the melting point of plastic. Do not
place items on the heater cover.

Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Potential pinch hazard
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Fisher Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Fisher Scientific’s compliance with this directive, the recyclers in your country and information on
these products will be available at www.fishersci.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.
Fisher Scientific Hybridization Incubators iiiFisher Scientific
Preface

Fisher Scientificiv Hybridization Incubators
Preface
Do You Need Information or Assistance
on Fisher Scientific Equipment?
The Fisher Scientific Sales Group can provide information on pricing and give you
quotations. We can take your order and provide delivery information on major equipment items or
make arrangements to have your local sales representative contact your. Our products are listed on
the internet and we can be contacted through our Internet home page.
1-800-766-7000 Customer Service
www.fishersci.com To order online
Fisher Scientific Product Service Support can supply technical information about proper
setup, operation or troubleshooting of your equipment. We can fill your needs for replacement parts
or provide you with on-site service. We can also provide you with a quotation on our Extended
Maintenance Program for our products.
Whatever products you need or use, we will be happy to discuss your applications. If you are
experiencing technical problems, working together, we will help you locate the problem and, chances
are, correct it yourself...over the telephone without a service call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is covered
by the Fisher Scientific Products warranty, we will arrange for the unit to be repaired at our expense
and to your satisfaction.
Regardless of your needs, professional service technicians are available by telephone to assist
you concerning our products Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time.
Please call or fax us at:
1-800-766-7000 Customer Service
1-800-926-1166 Orders Fax
International customers, please contact your local Fisher Scientific distributor.

Hybridization Incubators vFisher Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Rotisseries Available . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Packing List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Front Panel Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Rear Panel Nomenclature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
General Operation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Setting the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Power Up Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Setting Offset Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Setting the Keypad Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Reading / Resetting Temp Deviation . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Setting Temp Deviation On or Off . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Setting and Reading Temp Deviation . . . . . . . . . . . . . . . . . . . . . . . .5-6
Installing the Rotisserie into the Incubator . . . . . . . . . . . . . . . . . . .5-7
Preparing the Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Using the Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Loading the Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Setting the Rotisserie for Variable Axis . . . . . . . . . . . . . . . . . . . . . .5-9
Unloading the Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Using the Rocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Installing the Rocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Loading the Rocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11
Unloading the Rocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11
Section 1
Section 2
Section 3
Section 4
Section 5

vi Hybridization Incubators Fisher Scientific
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Tightening Loose Belt Tension . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Chemical BioReagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 6
Section 8
Section 7
Section 9

Hybridization Incubators 1-1Fisher Scientific
Overview
Section 1 Introduction
This manual provides information and procedures on how to unpack, set
up, and operate the Fisher Scientific Isotemp brand of Hybridization
Incubators.
This manual is intended to be used by Fisher customers responsible for
operating the hybridization incubator.
Fisher Scientific Isotemp Hybridization Incubators are designed to process
nucleic and protein blots derived from hybridization procedures. Incubator
heat is accurately controlled to precisely maintain the chamber
temperature. Temperature can be set between slightly above ambient and
85°C and is easily set by simple controls on the front panel. A large, bright
display shows actual temperature during operation. The incubator features
keypad protection, which prevents the set temperature from being
changed. This is useful when setting up a processing program where the
unit will be unattended.
An offset temperature can be programmed to compensate for the actual
temperature inside a typical sample loaded into the tubes. The incubator
can monitor and store the maximum deviation of the actual temperature
from the set temperature. Temperature deviation can be monitored and
saved during processing and recalled later. A separate safety thermostat
limits the incubator temperature to approximately 95°C in the event of a
temperature controller failure. An indicator on the front panel shows when
this condition exists.
A rotisserie holds the sample tubes and rotates to provide membrane
coverage. Rotisserie rotation speed can be varied by a knob on the front
panel. A jog control permits rotating the rotisserie in steps to insert and
remove tubes.
The incubator has a glass door for observing samples during processing.
Interior surfaces are stainless steel for high durability and low maintenance.
Small and medium same size units are stackable with an optional stacking
plate. The small incubator can be mounted under a cabinet or counter
with the optional mounting bracket.

1-2 Hybridization Incubators Fisher Scientific
Section 1
Introduction
Different size rotisseries accept different sample tube capacities:
• With the small unit, the supplied rotisserie accepts 4 of the 35x300
mm sample tubes or 8 of the 35x150 mm tubes. An optional
rotisserie accepts 2 of the 70x300 mm tubes.
• With the medium unit, the supplied rotisserie accepts 10 of the
35x300 mm tubes or 20 of the 35x150 mm tubes per incubator. An
optional rotisserie accepts 4 of the 70x300 mm tubes per incubator.
• The dual unit, accepts two rotisseries, identical to those describe for
use in the medium unit.
As an alternative to rotisseries, a rocker is available for agitating samples.
The rocker is an option on the medium and dual units and it replaces the
rotisserie.
Rotisseries Available
Models Available
Sample Tube Capacity Sample Tube capacity
at line voltage with supplied rotisserie with optional
rotisserie
Size 120 VAC 100 VAC 230 VAC Cont.
European 230 VAC UK 35x300 mm 35x150 mm 70x300 mm
Small 13-247-10 13-247-11 13-247-12 13-247-13 4 8 2
Medium 13-247-20 13-247-21 13-247-22 13-247-23 10 20 4
Dual 13-247-30 13-247-31 13-247-32 13-247-33 20 40 8

Hybridization Incubators 2-1Fisher Scientific
Section 2 General Specifications
Power Requirements: . .100 VAC ±10%,50/60 Hz,
. . . . . . . . . . . . . . . . . . .120 VAC ±10%, 60 Hz,
. . . . . . . . . . . . . . . . .230 VAC ±10%, 50/60 Hz
Small/medium unit: . . . 3.4 A@ 120 VAC, 1.8 A@ 230 VAC
Dual unit: . . . .6.8 A@ 120 VAC, 3.6 A@ 230 VAC
Ambient conditions: . . . . +16 to 40°C, up to 90% RH, non-condensing
Temperature range: . . . . . . . . .Ambient +7 to 85°C
Average uniformity: . . . . ±0.5°C at 55°C within the chamber; ±0.25°C at
55°C sample to sample
Heat-up time: . . .20 minutes from ambient to 65°C
Rotisserie speed: . . . . . . . . . . . . . . . . . . . . .5-15 rpm
Environmental Conditions
Maximum altitude: . . . . . . . . . . . . . . . . . . . .2000 m
Over-voltage Category: I . . . . . . . . . . . . .I (IEC 664)
Pollution Degree: . . . . . . . . . . . . . . . . .2 (IEC 664)
Exterior dimensions: W x L x H (nominal) . . . . . . .
Small: . . .18 x 16.5 x 17 inches (46 x 42 x 44 cm)
Medium: .18 x 20 x 20.5 inches (46 x 51 x 53 cm)
Dual: . . . .18 x 20 x 41 inches (46 x 51 x 104 cm)
Weight:
Small: . . . . . . . . . . . . . . . . . . . . . . . . .45 lbs (21 kg)
Medium: . . . . . . . . . . . . . . . . . . . . . . .55 lbs (25 kg)
Dual: . . . . . . . . . . . . . . . . . . . . . . . . .105 lbs (48 kg)

Hybridization Incubators 3-1Fisher Scientific
Section 3 Safety Information
The following labels are used on the device:
Label Appearance Function
Controls Power
ALARM
Controls line power to the unit.
Rotisserie Motor Controls the motion of the rotisserie motor.
Rotisserie Speed Controls the rotisserie’s speed of rotation.
Temperature Control These controls are associated with the heating function.
Used with temperature display (see below).
Indicators Temperature Display
Indicates the temperature of the incubator chamber. Used with
temperature controls (see above). Note The red bar to the left of
the display indicates a negative value. Used in calibrating the unit.
Alarm Indicates that the unit is operating in the alarm condition
(the indication is only seen during the alarm condition).

3-2 Hybridization Incubators Fisher Scientific
Section 3
Safety Information
These instructions contain important operating and safety information.
The user must carefully read and understand these instructions before
using the hybridization incubator.
The hybridization incubator has been designed to optimize function,
reliability, safety, and ease of use. It is the user’s responsibility to install
and use the hybridization incubator properly. Follow these safety
instructions regarding the hybridization incubator:
• The hybridization incubator must be used as described in this manual.
If used in a manner other than described in this manual, protection
provided by the device may be impaired.
• User must completely read, understand, and follow the instructions in
this manual before operating the hybridization incubator.
• This equipment is for indoor use only.
• If you plan to use the unit with any procedure that will give off fumes,
be sure to operate the unit in a fume hood or with proper ventilation.
• Connect the unit to a properly grounded electrical outlet of the correct
voltage and current handling capacity. Check the nameplate on the
back of the unit for the voltage and current rating.
• Do not remove or modify the grounded power plug. Use only properly
grounded outlets to avoid a shock hazard.
• Disconnect the unit from the power outlet before performing any
cleaning, maintenance, or service.
• The hybridization incubator is not intended for use with flammable
materials. If used with flammable materials, fire or explosion hazard
may result.
• If radioactive materials are used in processing, user must comply with
all regulations regarding the acquisition, use, and disposal of such
materials.
• Do not continue to use the incubator if the temperature controller
fails, as evidenced by the red ALARM indicator lighting.
• Use appropriate hand and eye protection when handling hazardous
chemicals.

Hybridization Incubators 3-3Fisher Scientific
Section 3
Section title
• Rotisserie and shaker accessory may pose potential pinch hazard.
Always turn rotisserie motor off prior to handling rotisserie or shaker.
• The interior of the incubator and its contents can reach temperatures
that can cause burns. Avoid contact. The unit can remain hot without
visual indication for some time after power is switched off.
Warning This unit is not explosion-proof. Do not use in the presence of
flammable or combustible materials; fire or explosion may result. Unit
contains components that may ignite such materials. Before operating
oven, always observe the following safety precautions:
• Fumes and spillage from acidic solutions cause corrosion of the
stainless steel chamber. Care should be taken to maintain a neutral
pH at all times.
• The heater for the oven is in the bottom of the unit. Surface
temperatures at the bottom cover of the unit may be higher than
set point temperature. For example: A plastic container on the
heater cover may become hot enough to melt/burn the container at
settings below the melting point of plastic. Do not place items on
the heater cover.
Caution DOUBLE POLE NEUTRAL FUSING. s
• The power entry module located on the rear of the unit contains two
fuses, one located in each side of the AC line.
• To ensure stability of Dual Models, secure unit to a wall using the
included wall bracket.
• Use caution when moving incubator. Note weight of unit to be moved
and obtain help if necessary. Tilt incubator to allow grasping under
bottom of unit at the sides or back. Do not lift by grasping under
plastic bezel at front of unit. Dual unit may require a third person to
prevent unit from tipping while being moved.

Hybridization Incubators 4-1Fisher Scientific
Section 4 Unpacking and Installation
Each hybridization incubator is shipped in a single carton. When
unpacking the unit, check each item against the packing list below. If
something is missing, contact Technical Services, identifying the part by
name and catalog number.
Part numbers are listed in the Replacement Parts and Accessory Items
sections located at the end of this manual.
Packing List Quantity
Small Medium Dual Item supplied
1 1 1 Hybridization incubator
1 1 2 Drip tray
1 1 2 Rotisserie
1 1 1 35mm x 150mm hybridization tube (Cat. No. 13-247-150)
1 1 1 35mm x 300mm hybridization tube (Cat. No. 13-247-300)
2 2 4 Spare tube clips
1 1 1 P Power cord
1 1 1 Instructions
1 1 1 Warranty card
0 0 1 Wall Bracket

4-2 Hybridization Incubators Fisher Scientific
Section 4
Unpacking and Installation
Location Follow these guidelines when choosing a location for the hybridization
incubator. The location must:
• Provide an adequate source of power. Check the label on the back of
the unit for voltage and current requirements.
• Provide minimum air clearance as follows: 0.5 inch (13 mm) from top,
2 inches (51 mm) from back, and 2 inches (51 mm) on each side.
• Provide adequate clearance for loading and unloading samples.
• Be level and capable of supporting the weight of the unit when loaded
with samples.
• Be free of drafts and wide ambient temperature variations such as near
a heater or air conditioning vents.
• Be near sufficient counter space for sample preparation, loading, and
unloading.
• Provide a fume hood if hazardous fumes are anticipated when using
the hybridization incubator.
1. Carefully unpack the unit. Check
contents against packing list.
2. Remove all packaging material
before using the incubator.
3. Place incubator on a stable, level
surface. To ensure stability of
Dual Models, secure unit to a wall
using the included wall bracket
(Figure 4-1)
4. Install rotisserie or rocker.
5. Install drip tray inside incubator
chamber.
6. Plug the power cord into a
suitable grounded electrical outlet.
7. Check out system operation before
using unit.
8. Complete and return warranty
card.
Installation
Procedure
Top rear edge of dual unit
Wall bracket
Wall
or
Top rear edge of dual unit
Wall bracket
Wall
Figure 4-1. Wall Bracket

Hybridization Incubators 5-1Fisher Scientific
Section 5 Operation
Front Panel Controls
and Indicators
Control or indicator What it does How to use
power switch Controls line power to unit. I = power on
0 = power off
rotisserie motor switch
Controls motion of rotisserie
off = stops rotisserie
on = runs rotisserie at the
speed set on rotisserie speed
knob
jog = moves rotisserie a frac-
tion of a turn each time switch
is pressed.
rotisserie speed knob
Controls speed of rotisserie
Turn knob to the desired
speed for the rotisserie. For
slower speeds, turn the knob
counterclockwise toward min.
For faster speeds, turn the
knob clockwise toward max.
temperature display Shows actual or set tempera-
ture, depending on actions
taken on other keys.
During power up, displays
actual temperature of incuba-
tor. If you press either of the
arrow keys, shows set temper-
ature.
Shows messages to indicate
menu functions.
Use in conjunction with menu
key to access other functions.
Shows status and error mes-
sages.
Take action depending on
message. (See Power Up
Messages)

5-2 Hybridization Incubators Fisher Scientific
Section 5
Operation
Front Panel Controls
and Indicators (cont.)
Control or indicator What it does How to use
temperature control
menu key Advances the display
through the functions.
Press the menu key to step through all
functions. While each message is dis-
played, you can set a value with the
arrow keys.
CAL = Temperature offset
The entered value is added to the
measured temperature to allow cali-
brating the sample temperature.
Pro = Keypad protection
Choose on or off. When on, set tem-
perature cannot be changed. When
off, set temperature can be changed.
dEn = Deviation enable
When on, the system stores the maxi-
mum temperature deviation from the
set temperature. When off, the devia-
tion will not be stored.
dE = Maximum deviation
from set temperature since
the last time the value was
reset.
When deviation is enabled (see
above), display shows the maximum
deviation temperature since this func-
tion was last enabled. If deviation
enable is set to off, this function is
skipped. Pressing either arrow key
while viewing the deviation resets the
value to zero.
temperature control
arrow keys
Display or change the set
temperature
Press either arrow key once to display
the set temperature for two seconds.
Hold the up arrow key to increase the
set temperature. Hold the down arrow
key to decrease the set temperature.
ALARM indicator
ALARM
Shows when there is an
alarm condition
If the ALARM indicator lights, switch
off power and call Technical Services.
Primary heater control has failed and
unit is operating on safety thermostat.
Temperature is approximately 95°C.
Item What it does How to use
power entry module/
line filter/fuse holder
Accepts power cord for providing line
power to unit. Filters incoming and
outgoing power line noise. Holds fuses
for over-current protection.
Plug mating end of power
cord into this connector. Plug
other end into suitable power
outlet.
Rear Panel
Nomenclature

Follow this general procedure when operating the hybridization incubator:
1. Read, understand, and follow all safety information.
2. Install rotisserie or rocker. See “Installing the Rotisserie into the
Incubator” and “Rocker Installation” in the Operation section.
3. Install drip tray and leave in place at all times.
4. Power up system. See “Power Up” in the Operation section.
5. Set temperature as desired for processing and allow incubator to reach
and stabilize at desired temperature. See “Setting the Temperature”
and “Setting the Temperature Offset” in the Operation section.
6. Load samples into rotisserie or rocker. See “Loading the Rotisserie” in
the Operation section. To set the rotisserie for variable axis, see details
under “Using the Rotisserie” and “Loading the Rocker.”
7. Run processing procedure as desired. To set keypad protection, see
“Setting the Keypad Protection” in this section. For details on
temperature deviation, see “Setting and Reading Temperature
Deviation” in thsi section.
8. At conclusion of processing, unload samples from rotisserie or rocker.
For details on rotisserie and rocker unloading, see “Unloading the
Rotisserie” and “Unloading the Rocker” in the this section.
9. Clean incubator for next use. See “Cleaning the Unit” in this section.
Follow this procedure to power-up the incubator.
1. Set the power switch to the I position. Display alternates between oFF
and the measured temperature. If you get any other message, see
“Power Up Messages” below.
2. Press any key to stop the oFF display. The system displays the actual
temperature inside the incubator.
Hybridization Incubators 5-3Fisher Scientific
Section 5
Operation
General Operation
Procedure
Power Up