Fisher Scientific Fisher Isotemp 307C User manual

Fisher IsotempLow Temperature Incubator
Model 307C Catalog Number 11-679-25C
Fisher Low Temperature Incubator offers laboratories precise
temperature control over the-10°C to 60°C range.
Performance meets requirements for the preservation of
vaccines, biologicals, incubation of bacterial cultures, and
determination of bio-chemical oxygen demand of sewage. The
extended operating temperature range also includes
temperatures associated with drug stability projects, dairy
product evaluation and entomological studies.
The spacious chamber accommodates up to 300 standard
BOD bottles, or similar containers. The 5 reinforced shelves
can handle up to 60 lbs of sample weight each.
Features include:
Ø5 Removable shelves
Ø1 Removable storage basket
Ø6 Molded door shelves
ØDoor Lock with key
ØMicroprocessor based temperature controller, with
temperature readout to .1°C
Ø3 Cooling control modes: High precision with cooling, High
precision without cooling and Frost free with variable cooling
ØSolid state relay for heater
ØOver/ under temperature safety relay and alarm Over
temperature thermostat
ØCompressor relay for energy conservation
ØCompressor overload relay
ØRTD temperature probe
ØProtected setpoint mode to avoid accidental change
ØConvenience outlet inside chamber, 2 amp
ØInterior light with door switch
WARNING
•This manual must be carefully read and thoroughly
understood before operating the unit, failure to follow
directions or precautionary measures could result in
serious adverse effects.
•This equipment must be used only as specified in these
instructions.
•This equipment is intended for indoor use only.
•This equipment must be earth grounded for safe operation.
•Maximum ratings of accessories that may be plugged into
convenience outlet: Load current 2 Amps. @ 115VAC,
Leakage Current 250 micro Amps.
CAUTION
The aluminum evaporator and other portions of these units
should not be exposed to the corrosive effects of acidic or
caustic materials. Extreme care must be exercised if such
materials are stored within to prevent voiding the warranty.
This Incubator is NOT suitable for flammable material storage.
SPECIFICATIONS
Power Requirements:
115V, 8.9A, 60 Hz (Including 2 A for convenience outlet)
Dimensions:
Height: 70 in (179cm)
Width: 32 in (81cm)
Depth: 28.5 in (72cm)
Volume: 20.3 cu ft (0.58m3)
Shelf Data: 5 in chamber, 6 on door, 1 basket
Capacity: 300 BOD bottles
Shipping Weight:
283 lb. (128 kg)
Operating Environment:
Temperature Range: 10°C to 40°C
Humidity Range: 0% to 90% RH
Maximum Altitude: 6600 ft (2 km)
Air clearances: Back, top and sides 3.0” minimum
Overvoltage Category II (IEC664)
Pollution Degree 2 (IEC664)
Performance Characteristics:
Operating Range: -10°to +60°C
Uniformity: ±0.5°C1
Stability: ±0.2°C1
Display Readability: 0.1°C
1Per ASTM method E 1292-94, cooling modes 00 and 100
PACKING LIST
Qty Item
1 Low Temperature Incubator
1 Instructions
UNPACKING and INSPECTION
NOTE: If damage is observed,keep the shipment intact
(including the carton and all packing material) and file a claim
with the final carrier.
INSTALLATION
The Low Temperature Incubator should be installed on a level,
stable floor. Use the adjustable feet to level the incubator.
Locate the unit so that there is at least 3 inches of clearance
around the sides, top and back to ensure sufficient ventilation.
Note: When cooling is enabled, heat is dissipated on sides of
unit resulting in side temperatures of 35 to 55°C. To prevent
heat build up within the unit, do not install it where it will be
exposed to strong sunlight, nor near radiators, furnaces, or
other sources of heat. Do not install the incubator where
flammable or corrosive atmospheres may exist. The Low
Temperature Incubator is not to be used out-of-doors or where
excessive moisture or contamination could cause degrading of
mechanical or electrical components.
1. Unbolt and remove the wooden slats underneath unit.
2. Remove all protective tape from doors and shelves.
3. Clean interior and exterior with warm soap and water.
Shipping tape residue can be removed using isopropyl alcohol.
4. Uncoil line cord from rear of unit. Connect the plug to a
power outlet that complies with the electrical requirements
specified on the unit's label and with proper safety ground
connection. If line cord replacement is necessary, use CSA
types: SJ, SJO or SJT, 16 GA, 3 cond. or equivalent.

CONTROLS
Power ON/OFF Switch: Located on back wall of chamber, is
used to turn power (1) ON or (0) OFF to entire unit.
Alarm Switch:
Located in kick-panel in the front, is used to enable or disable
alarm buzzer.
Keys: Located on front panel.
MENU -Used to access controller menus.
SET -Used to display setting.
∆INCREASE setting.
∇DECREASE setting.
DISPLAY and INDICATORS
DISPLAY: (On front panel)
Displays chamber and set point temperatures in °C and
prompts from menu.
INDICATORS: (On front panel)
DELAY: Lights when delay start timer for compressor is
timing. Will begin timing when power is first applied or when
cooling compressor is turned off.
ALARM: Indicates that chamber temperature is not within
limits and the safety relay is opened.
COOL: Lite when cooling compressor is on.
HEAT: Lights when controller demands heat.
INDICATORS: (In kick-panel grille)
Temperature Alarm:
Indicates alarm is activated.
GENERAL FUNCTIONAL CHECKS
1. Apply power by placing the Power ON/ OFF switch (Located
inside chamber) to I (ON) position. The Controller, on front of
door, will display L , then, 88.8 (to test display), then begins
reading actual chamber temperature.
Note: When power is first applied, if display indicates S
instead of L, a switch setting on the controller must be changed.
Refer to Replacing Temp Controller TC1.
2. After 3 seconds, the alarm LED on the controller should
extinguish and the safety relay will close applying power to the
heater, fan, compressor relay and convenience outlet.
3. Press and hold MENU key, after 3 sec. the display will
indicate CAL. Release and press MENU again to display Pct,
press SET, display should indicate 100. Press and release
MENU again to indicate Pro, press SET, display should
indicate 00, press and release MENU to return to normal control
mode. Press SET to display control setpoint, display should
read 25.0. If the control setpoint or any of the other parameters
aren't set correctly, use the∆or ∇keys while pressing SET to
change the setting.
4. The DELAY LED will be lit for 7 minutes after power is first
applied, the COOL LED will be out during this delay period.
When the DELAY LED goes out, the COOL LED will light and
the compressor relay will close starting the compressor.
5. Allow unit to stabilize for 1 hour at 25°C, to ensure that all
systems are functioning.
OPERATION
Perform the procedure under General Functional Checks if
incubator is new or hasn't been operated for a long period of
time.
Controller operation:
Usually the only controller setting that is necessary to change is
the chamber temperature control setpoint. Other parameters of
the controller may be changed by accessing them through the
menu key, covered later.
Changing Chamber Temperature Control Setpoint
1. The chamber temperature is continuously displayed, unless
a key is pressed.
2. To display the setpoint temperature, press the SET key. The
setpoint temperature will remain on the display for 1 sec. after
the SET key is released.
3. To change the setpoint temperature, press and hold SET
while pressing the∆or ∇keys to change the setting. (Note that
when either ∆or ∇keys are first pressed, the display will begin
to change slowly at first, then increase change rate after a few
seconds.)
4. Select the desired chamber setpoint then release all keys.
The controller display will revert back to actual chamber
temperature after a few seconds. Allow up to an hour for unit to
stabilize at the new setpoint temperature.
After the incubator temperature has stabilized, place samples
into incubator chamber. For best results, arrange samples
evenly throughout the chamber. Liquid samples should be
covered to prevent evaporation and eventual frost build up on
evaporator coils, particularly when operating below ambient.
The Low Temperature Incubator has an internal 2 amp.
convenience outlet inside chamber to operate devices such as:
shakers, rotators, photosynthesis lights, recording
thermometers etc.
Accessing Controller Menus:
The temperature controller has three menu selections that can
be accessed by pressing and holding the MENU key for 3
seconds. To access menu parameter, press and hold the SET
key. To change the parameter, use the∆or ∇keys.
CAL Menu-Calibration Menu is used to change the
temperature offset value to correct for differences in chamber
temperature and the displayed temperature. Range-5°to 5°C.
See Calibration Procedure.
Pct Menu-(Percent Cooling Menu) Used to select one of three
cooling modes:
100% Mode-Compressor runs continuously which provides
high precision control (+/-.2°C Stability) over entire temperature
range.
50% Mode-Compressor runs approximately half the time
which conserves power and also prevents and eliminates frost
buildup inside chamber. Stability is +/-1.5°C in this mode.00%
Mode-Compressor is completely off. This mode is ideal for
incubating samples at temperatures 10°C or higher above
ambient (35°C to 60°C). It provides the greatest power savings
yet still maintains the advertised stability.
Pro Menu-Protection Menu -Used to select the Protection

mode of operation that determines when the alarm is activated
and if the control setpoint can be changed. Range 0.0 to 0.2.
0.0 Mode-Normal mode that allows the user to change
setpoint. Alarm activates if chamber temperature (chamber)
and setpoint temperature (setpoint) are as follows:
chamber ≤15.0°C and chamber < (setpoint -3) or
chamber ≥40.0°C and chamber > (setpoint+3)
While ALARM is activated, the safety relay is opened
interrupting power to the heater, fan, convenience outlet and
compressor. When chamber temperature returns to acceptable
limits, the ALARM will automatically terminate and power is
reapplied. In order to allow changing of setpoint and eliminate
nuisance alarms, an alarm by-pass takes effect when the
setpoint is changed or when power is first applied. The alarm
by-pass works as follows: Present chamber temperature is
stored as a nominal alarm temperature (nominal). If chamber <
setpoint then alarm temperature (alarm) = nominal -3. If
chamber > setpoint then alarm = nominal +3. If chamber should
go beyond alarm or < -13 or > 63 then the ALARM will activate.
This alarm by-pass is active until chamber reaches setpoint
±1°C.
0.1 Mode-Same as 0.0 Mode, except that the setpoint is
locked and can't be changed.
0.2 Mode Setpoint is locked, plus, the ALARM will activate
and the safety relay will open as follows:
chamber < (setpoint -3) or chamber > (setpoint +3)
In order to use this mode of protection, modes 0.0 or 0.1 will
have to be used to reach the setpoint temperature. Once the
chamber temperature reaches the setpoint and is stable,
protection mode 0.2 can be set.
Note! If the chamber temperature falls outside the setpoint by
±3°C, the ALARM will activate and safety relay will interrupt
power. This mode can only be used if the door is to remain shut
and other disturbances don't effect the chamber temperature.
The audible alarm, located in the kick-panel grille, can be turned
onor off by using a small rod, such as a plastic pen, to push the
switch either ON or OFF. This doesn't effect the safety power
relay operation or alarm lights.
As an added safety feature, the incubator has a thermostat in
the heater compartment that limits the upper chamber
temperature to 65°C.
MAINTENANCE
WARNING
To reduce the risk of electric shock, disconnect from
power source before servicing.
Before re-applying power, after maintenance is complete,
check to ensure that safety ground is in-tact and making a good
connection.
CLEANING
Immediately clean all spilled materials from the incubator and
wipe dry. If necessary, moisten a cloth with soap and water and
clean inside and out. Do not use any harsh chemical cleaners.
Do not attempt to clean the incubator while the unit is plugged
into a power source.
DEFROSTING
Periodic defrosting may be necessary depending on operating
temperature, ambient humidity, moisture from samples and
cooling mode. Time between defrosts may be from 30 days to
years.
Suggested defrost methods.
Method I: For control setpoints down to 10°C, this method is
the least disruptive, if samples can withstand temperature
stability of +/-1.5°C for 24 hours. Samples may remain in
chamber during this procedure.
1. (Refer to Accessing Controller Menus) Press and hold MENU
key until CAL is displayed, then release and press MENU again
until Pct is displayed.
2. Select 50 in the Pct menu then press MENU key twice to
return control mode.
3. Allow incubator to remain in this mode for about 24 hours or
until frost disappears.
4. Use the MENU key to re-establish the previous selection for
Pct (i.e. 100).
Method II:
1. Remove any samples that may be damaged by temperatures
up to 35°C.
2. Refer to Accessing Menu section of manual and set Pct
parameter to 00.
3. Place Setpoint to 35.0°C.
4. Allow temperature to stabilize, for 1 hour.
5. After 1 hour, remove power from unit. Open door and wipe
up any excess moisture on floor of chamber with paper towels
or sponge.
6. Apply power and set desired operating parameters back into
controller. After chamber temperature stabilizes, place
samples previously removed back into unit.
CALIBRATION
If it becomes necessary to calibrate temperaturecontroller,
perform the following:
1. Place an accurate temperature measuring device in the
geometric center of the chamber.
2. Set the controller to the desired operating temperature and
allow to stabilize for 2 hours.
3. Note the chamber temperature onthe controller display and
the temperature at the geometric center.
4. Subtract the chamber display reading from the geometric

center reading.
5. Enter into the Calibration Mode as described in Accessing
Menu.
6. Press and hold SET key, using the∆or ∇keys, set the
calibration parameter to the value determined in step 4.
7. Allow unit to stabilize for 1 hour and check that controller
value and geometric center value agree ±.2°C. Repeat
calibration if necessary.
TROUBLE SHOOTING and REPAIR
Controller Errors and Alarms
Display Safety Alarm =Meaning
EEE/99.9 XX=Open Sensor
EEE/99.8 XX=Shorted Sensor
EEE/TEMP XX=Temp Limit
Temp Limit = Temp (+/-3°C or more from setpoint) &
(> 40°C or <15°C)
Replacing Temp. Controller:
1. Disconnect incubator from power source.
2. Remove front panel bezel by removing four screws in top and
bottom of bezel.
3. Remove four screws that mount controller to mounting
bracket.
4. Note wire colors and positions on TB1 and TB2, then remove
all wires from TB1 and TB2 and old controller.
5. Locate switch DS1 on lower left corner of new controller and
set the switches as follows:
Set DS1-1 (A) to on (down) .
Set DS1-2 (B) to off (up).
6. Attach wires previously removed from old controller to new
controller.
7. Mount new controller and bezel, then apply power.
8. Refer to Calibration section of this manual, to calibrate new
controller.
Replacing RTD Temp. Sensor:
1. Disconnect incubator from power source.
2. Refer to Replacing Temp. Controller, but only remove sensor
wires from TB2.
3. Open incubator door and remove screw that holds Temp.
Sensor on door shelf bracket.
4. Remove old sensor, then place new sensor on bracket.
5. Connect new sensor wires to controller then mount controller
and bezel.
6. Apply power, then refer to Calibration section.
Replacing SSR1, K1, K2 or T1:
1. Disconnect incubator for power source.
2. Remove six screws from back panel then remove back
panel.
3. Locate device to be replaced on bottom panel then remove
mounting screws.
4. Unplug quick connect terminals, noting positions.
5. Place quick connects on new device and mount on panel.
6. Replace back panel, then apply power.
7. Refer to General Functional Checks to ensure that new
device is operating.
Replacing FAN, S1, or THM1:
1. Disconnect incubator from power source.
2. Remove basket and shelves from chamber area.
3. Remove nine screws from heater cover at rear of chamber.
4. Rotate heater cover to right side of chamber.
5. Fan is located on back wall and S1and THM1 are on switch
panel attached to heater cover.
6. Replace desired part, then coat electrical connections with
RTV102 or other electrical insulator.
7. Re-assemble in opposite order as previously described. Be
sure to install gaskets on either side of heater cover.
8. Apply power, refer to General Functional Checks to ensure
that new device is operating.
Replacement Parts:
TC1 Controller SPN102214
Sensor, RTD SPN88613
SSR1 Relay SPN88616
K1 Safety Relay SPN102260
K2 Comp Relay SPN102259
T1 Transformer SPN88678
THM1 Thermostat SPN88612
FAN1 Motor SPN46367
S1 Switch SPN83373
Customer Service: 1-800-766-7000
Technical Assistance: 1-800-943-2006
Fisher Service Division: 1-800-395-5442
Fisher Scientific
2000 Park Lane
Pittsburgh, PA 15275 USA
Instruction Manual Part # 88732 Rev. E