Fisher Scientific 15-460-2Q User manual

Fisher Scientific Isotemp
Economy Water Baths
Installation and Operation Manual
103412 (7002200) Rev. 5
© 2010 Fisher Scientific Inc.
All rights reserved
Printed in U.S.A.
Isotemp

Fisher ScientificFisher Scientific
MANUAL NUMBER 103412 (7002200)
5 31076 2/11/15 Added kerosene note ccs
4 30931 4/9/14 Updated lids and hinges for the 5 liter baths in the Parts List (pg 7-2) ccs
3 29436 4/7/14 Added Safety Specs to Section 3 ccs
2 30371/BA-816 10/3/13 Removed discontinued models from model lists ccs
1 29544/29545 2/20/13 Added “Indoor use only” to Specifications ccs
0 -- 4/29/10 Transfer to Marietta (was 103412 11/2/09) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Isotemp Economy Water Bath i
Models covered in this manual
Catalog Number Model Number Specific Information Voltage
15-460-2Q (2228)
102
2L 120V
15-460-3Q (2235) 2L 230V
15-460-2SQ (2230) 2L 120V
15-460-3SQ (2236) 2L 230V
15-460-5Q (2239)
105
5L 120V
15-460-6Q (2240) 5L 230V
15-460-10Q (2223)
110
10L 120V
15-460-11Q (2224) 10L 230V
15-460-15Q (2226)
115
5L/10L, dual chamber 120V
15-460-16Q (2227) 5L/10L, dual chamber 230V
15-460-20Q (2231)
120
20L 120V
15-460-21Q (2232) 20L 230V
15-460-28Q (2233)
128
28L 120V
15-460-29Q (2234) 28L 230V

Fisher Scientificii Isotemp Economy Water Bath Fisher Scientific
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Fisher Scientific makes no representations or warranties with respect to this manual. In
no event shall Fisher Scientific be held liable for any damages, direct or incidental, arising out of or related to the
use of this manual.
©2010 Fisher Scientific. All rights reserved.

Fisher Scientific Isotemp Economy Water Bath iiiFisher Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Fisher Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Fisher Scientific’s compliance with this directive, the recyclers in your country and information on
these products will be available at www.fishersci.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Fisher Scientificiv Isotemp Economy Water Bath Fisher Scientific
Preface
Do You Need Information or Assistance
on Fisher Scientific Equipment?
The Fisher Scientific Sales Group can provide information on pricing and give you
quotations. We can take your order and provide delivery information on major equipment items or
make arrangements to have your local sales representative contact your. Our products are listed on
the internet and we can be contacted through our Internet home page.
1-800-766-7000 Customer Service
www.fishersci.com To order online
Fisher Scientific Product Service Support can supply technical information about proper
setup, operation or troubleshooting of your equipment. We can fill your needs for replacement parts
or provide you with on-site service. We can also provide you with a quotation on our Extended
Maintenance Program for our products.
Whatever products you need or use, we will be happy to discuss your applications. If you are
experiencing technical problems, working together, we will help you locate the problem and, chances
are, correct it yourself...over the telephone without a service call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is covered
by the Fisher Scientific Products warranty, we will arrange for the unit to be repaired at our expense
and to your satisfaction.
Regardless of your needs, professional service technicians are available by telephone to assist
you concerning our products Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time.
Please call or fax us at:
1-800-766-7000 Customer Service
1-800-926-1166 Orders Fax
International customers, please contact your local Fisher Scientific distributor.

Isotemp Economy Water Bath vFisher Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Assembly and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Filling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Setting the Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Setting the Limit Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Procedure 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Procedure 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Emptying and Cleaning the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Schematic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 8
Section 7

Isotemp Economy Water Bath 1-1Fisher Scientific
Section 1 Introduction
Congratulations on the purchase of your new Fisher Scientific Isotemp
Economy Water Bath.
Isotemp Water Baths offer the versatility needed to handle virtually any
clinical laboratory procedure - incubation, inactivation, agglutination - as
well as most serological, pharmaceutical, biomedical, and industrial
procedures.
These water baths are available with chamber capacities of 2, 5, 10, 20,
and 28 liters. The 2 liter unit is available configured with either a standard
or shallow chamber. Also, a dual chamber unit is available with 5 and 10
liter chambers, with independent controls for each. All units are available
in either 120 or 230 volt models.
The baths work equally well with water or bath oil as the medium. Most
models offer the same temperature ranges: ambient to 75°C without the
supplied cover, and ambient to 100°C with the cover in place. The
temperature range for Model 102 extends from ambient to 85°C without
the cover, while the range for Model 102S is from ambient to 70°C.
Bath temperatures are controlled by a hydraulic thermostat for reliable,
trouble-free operation. Dual thermostats - one controlling the set point
temperature and the other operating as a temperature limiting - reduce the
possibility of overheating. Although not designed to operate dry, the bath
will not be damaged if it is allowed to run dry.
An energy-saving removable cover helps to reduce evaporation while
helping to maintain a uniform, constant temperature. The cover also
allows the water bath to reach higher temperatures. The hinged, see-
through gable cover features unique “fins” that keep hands away from hot
vapors for easier, safer opening. The cover stays open at the 90° position
or, because of its quick-attach hinge, can be lifted off completely to
accommodate large glassware.
All controls are conveniently located on the front panel for easy access.
The water bath features corrosion resistant construction throughout to
withstand the rigors of daily lab use. A plastic rim remains cool to the
touch even when operating the bath at maximum temperature.

Isotemp Economy Water Bath 2-1Fisher Scientific
Section 2 Safety Information
To avoid electrical shock, always:
• Connect the water bath to a properly grounded electrical outlet of the
correct voltage and current handling capacity. Check the nameplate on
the back of the unit for the voltage and current rating.
• Disconnect the unit from the power outlet prior to maintenance and
servicing. Note 230V units have double pole, neutral fusing.
To avoid personal injury and property damage:
• Do not use in the presence of flammable or combustible materials; fire
or explosion may result. This device contains components which may
ignite such materials.
• Do not remove or modify the grounded power plug. Use only properly
grounded outlets to avoid a shock hazard. This unit is not rated for use
in hazardous atmospheres.
• Do not continue to use the bath if the Temperature Control fails
(amber indicator cycling on and off) or if the Limit Control fails
(amber indicator will not light even if Limit Control is turned fully
counterclockwise).
• Select a fluid that is not corrosive and is not flammable. The following
fluids are not recommended and may damage the unit:
- Chlorides or bleach.
- Strong concentrations of any acid.
- Strong concentrations of any salt.
- Weak concentrations of hydrochloric acid, hydrofluoric acid,
hydrobromic acid, hydroiodic acid, sulfuric acid, or chromic acid.
- Weak salt solutions containing sodium chloride, calcium chloride,
chromate or chromium compounds.
- Most photographic solutions.
- High Purity water (deionized water >1M ohm)

2-2 Isotemp Economy Water Bath Fisher Scientific
Section 2
Safety Information
To avoid personal injury and property damage (continued):
• Do not use a flammable liquid. A fire hazard may result. This unit is
not explosion proof. Unit contains components which may ignite such
materials.
• Use appropriate hand and eye protection when handling hazardous
chemicals.
• The interior of the unit can reach temperatures that can cause burns.
Avoid contact. The unit can remain hot without visual indication for
some time after power is turned off.
• If the water bath is to be used with any liquid that will give off fumes,
be sure to operate the water bath in a fume hood or with proper
ventilation.
• Use the cover to reduce evaporation and to permit reaching higher
temperatures.
• Hot liquids pose a burn hazard. Be careful not to reach into the bath
when it contains hot liquids. Also be careful of steam rising from hot
liquids.
• The unit is intended to be operated with liquid in the chamber.
However, it will not be damaged if it temporarily runs dry.
• Do not use in highly corrosive atmospheres; corrosive fumes and
spillage may damage the unit and its internal components, creating a
shock hazard.
• Fumes from acidic solutions cause corrosion of the stainless steel
reservoir. Care should be taken to maintain a neutral pH at all times.
• Refer servicing to qualified personnel.
• Do not place containers directly on bottom of chamber. The bottom
can get extremely hot if no liquid is in the chamber. Always use the
diffuser tray.
• The thermometer contains kerosene and is not expected to be a health
hazard in the normal intended use of the thermometer.

Isotemp Economy Water Bath 3-1Fisher Scientific
Section 3 Specifications
These are nominal specifications. Fisher Scientific reserves the right to
change specifications or designs at any time without incurring obligation.
Temperature range . . . . . . . . . . . . . . . . . . . . . . . . . .
Cover Open: Ambient to 75°C (model 102 to 85°C, model 102S to
70°C)
Cover closed: . . . . . . . . . . . . . .Ambient to 100°C
Uniformity . . . . . . . . . . . . . . . . . . . .±0.2°C at 37°C
. . . . . . . . . . . . . . .±0.5°C at 37°C (shallow pan)
Stability . . . . . . . . . . . .±0.2°C at 37°C (with cover)
Safety Specifications
Indoor use only
Altitude . . . . . . . . . . . . . . . . . . . . up to 2000 meters
Ambient Temperature . . . . . . . . . . . . . .0°C to 40°C
Ambient Humidity . . . . . . 0 - 95% non-condensing
Voltage Requirements 120VAC or 230VAC nominal
Voltage Fluctuations . . . . .±10% of nominal voltage
Installation Category II
Pollution Degree 2
Certification
Declaration of Conformity is available from the factory

3-2 Isotemp Economy Water Bath Fisher Scientific
Section 3
Specifications
Catalog Number Model Number Specific Information Voltage
15-460-2Q (2228)
102
2L 120V
15-460-3Q (2235) 2L 230V
15-460-2SQ (2230) 2L 120V
15-460-3SQ (2236) 2L 230V
15-460-5Q (2239)
105
5L 120V
15-460-6Q (2240) 5L 230V
15-460-10Q (2223)
110
10L 120V
15-460-11Q (2224) 10L 230V
15-460-15Q (2226)
115
5L/10L, dual chamber 120V
15-460-16Q (2227) 5L/10L, dual chamber 230V
15-460-20Q (2231)
120
20L 120V
15-460-21Q (2232) 20L 230V
15-460-28Q (2233)
128
28L 120V
15-460-29Q (2234) 28L 230V

Isotemp Economy Water Bath 4-1Fisher Scientific
Section 4 Unpacking and Installation
The water bath is shipped in a single carton. When unpacking the unit,
check each loose item against the packing list below. Should a shortage
exist, notify your Fisher Scientific distributor, identifying the part by name
and catalog number.
Note If there is shipping damage, keep entire shipment intact - retaining
the carton and all packing material - and file a claim with the final carrier.
Usually the firm will send an investigator to ascertain liability. s
Note The thermometer contains kerosene and is not expected to be a
health hazard in the normal intended use of the thermometer.
Packing List
Qty Item Supplied
1 Water Bath
1 Cover Assembly
1 Diffuser Tray
1 Thermometer Clip and Grommet
1 Thermometer (120 V only. Not included with 230 V units)
1 Instructions

4-2 Isotemp Economy Water Bath Fisher Scientific
Section 4
Unpacking and Installation
Follow these steps to assemble and install the water bath before operation:
1. The location must:
• Be indoors.
• Provide an adequate source of power. Check the label on the back
of the unit for voltage and current requirements.
• Provide adequate clearance to insert samples.
• Be level, fixed, and capable of supporting the weight of the unit
when filled with liquid and samples.
• Be free of drafts and wide ambient temperature variations such as
near a heater or air conditioning vents.
• Provide a fume hood if hazardous fumes are anticipated when
using the water bath.
• Be convenient to a sink for filling and draining the water bath.
2. Install the cover by inserting the hinge
into the lip at the rear of the bath.
Angle the cover up while pushing
down to lock the hinge in place under
the stainless steel tank lip. Remove the
cover by lifting the lid and pushing the
hinge towards the back to release it
from under the tank lip. Covers on the
2 liter shallow, 5 liter, and 10 liter
model baths can also be installed and
removed by sliding the hinge into
place under the rim and tank lips from either side.
3. Connect the line cord to a suitable grounded electrical outlet.
Assembly and
Installation

Isotemp Economy Water Bath 4-3Fisher Scientific
Section 4
Unpacking and Installation
Follow these steps to fill the unit in preparation for use:
1. Fill the water bath with distilled water or oil so that the liquid level is
approximately 1½ inches (¾ inch with 2 liter shallow model) from the
top of the tank when full anticipated load is placed in bath.
For best operation of the equipment, fill the unit with sterilized
distilled water or equivalent. The acceptable resistivity range is 50K to
1M ohms (conductivity 1 to 20 micro Siemens).
2. If a thermometer is used, use the clip provided to hold the
thermometer to the side of the bath. Slide the O-ring on the
thermometer to position the thermometer to the proper depth.
3. When closing the cover, place the thermometer along the front edge. A
notch along the front of the cover clears the thermometer and clip
with the cover closed.
4. For optimum results, the same fluid level should be maintained
throughout the operating period.
5. If using water, algicide may be added to reduce algae formation.
Follow the instructions supplied with the algicide.
Note If the bath has been used previously, the tank should be thoroughly
cleaned before refilling. s
Caution Using chlorinated tap water or additives that contain chlorine will
void the manufacturer warranty. Similarly, high purity (deionized) water
that does not fall within a resistivity range of 50K to 1M ohm will void
the manufacturer warranty. If you should have any questions, please
contact Technical Services. s
Note Deionized water is not recommended and may cause rusting and
pitting in the reservoir. s
Filling the Unit

All controls and indicators are located on the front panel for ease of
operation.
Power:
Controls line power to unit. Set to 1 position to turn on power, 0 posi-
tion to turn off power.
Temperature Control:
Serves as the primary temperature control for the bath. Turns the heater
on or off to maintain the bath at the set temperature.
Limit Control:
Sets the backup temperature to the primary setpoint; should be set at a
slightly higher temperature than the Temperature Control setpoint to
give safety control in the event that the primary control fails.
Temperature Control Indicator:
The green indicator illuminates to show when heat is being supplied to
the chamber; cycles on and off as heat is requester by the controller.
Limit Control Indicator:
The amber indicator illuminates to show the the Limit Control is regu-
lating the temperature rather than the primary Temperature Control.
Note If the bath has been used previously, the tank should be thoroughly
cleaned before refilling. s
Note The water bath, full of water (within 1-1.5" of top of tank), with lid,
is designed to reach 37°C in less than 60 minutes or 90°C in less than 180
minutes. s
4-4 Isotemp Economy Water Bath Fisher Scientific
Section 4
Unpacking and Installation
Controls and
Indicators

Isotemp Economy Water Bath 5-1Fisher Scientific
Section 5 Operation
Follow these procedures for the operation of the unit.
• Power up and initial indication.
• Setting the temperature control.
• Setting the limit control.
• Emptying and cleaning the unit.
Follow these steps to turn On the unit to prepare it for use:
1. With the unit connected to an appropriate power source, set the power
switch to the ON position.
2. The green indicator will illuminate when the unit is applying heat to
the bath. This indicator will cycle on and off during normal operation.
Follow these steps to set the Temperature Control so that it maintains the
desired temperature.
1. Turn Limit Control knob fully clockwise.
2. Turn the Temperature Control to a setting which approximates the
desired temperature. Measure the bath temperature with the supplied
thermometer or other temperature measuring device. Allow the bath
temperature to stabilize, then readjust the control knob as necessary to
achieve the proper temperature.
3. Set the Limit Control according to the procedure below.
Note The safety control is not as accurate a control as digital control. s
Note The thermometer contains kerosene and is not expected to be a
health hazard in the normal intended use of the thermometer. s
Power Up
Setting the
Temperature Control

5-2 Isotemp Economy Water Bath Fisher Scientific
Section 5
Operation
Proper setting of the Limit Control is important. The Limit Control must
be set far enough above the control temperature such that it does not
operate unless the Temperature Control malfunctions. Wide temperature
swings are an indication that the two controls are set too close.
There are two methods that can be used to set the Limit Control. Follow
the steps in either Procedure 1 or Procedure 2 to set the bath’s Limit
Control.
1. After the bath has stabilized at the desired set point temperature, note
the cycling of the heater as indicated by the green lamp.
2. Note the length of time that the green lamp is lit. Depending upon set
point and ambient temperature, the lamp will cycle on for a few
seconds and then off for a period of time.
3. While the green lamp is lit, rotate the Limit Control knob counter-
clockwise until the amber lamp illuminates (the green lamp will turn
off), then quickly clockwise again until the amber lamp turns off (the
green lamp will come back on).
4. Observe a few cycles of the green lamp. If the yellow lamp illuminates,
turn the Limit Control knob clockwise until the unit cycles without
the yellow lamp lighting.
1. Using the Temperature Control setting instructions above, set the
water bath 1 to 5° above desired control temperature.
2. Wait until the bath temperature stabilizes.
3. Turn the Limit Control knob counterclockwise and listen for the click
of the control. Turn the knob clockwise and slowly listen for the click.
Repeat this step as necessary each adjustment being less than the last
one.
4. Once a narrow band is established in Step 3, turn the Temperature
Control up to call for a higher temperature (heater on, green lamp on).
The amber lamp will turn on when the limit open turning off the
heater.
5. Without adjusting the Limit Control, set the Temperature Control to
the desired temperature below the limit control.
Setting the Limit
Control
Procedure 1
(Use either Procedure 1 or 2)
Procedure 2
(Use either Procedure 2 or 1)

6. Check that the amber lamp does not illuminate during the control
mode. If it does the Limit Control is set too close to the Temperature
Control. Start back at Step 1 to reset the Limit Control.
Note The amber limit lamp has no power to illuminate when the
temperature control is not calling for heat (green lamp off). The green
lamp goes off when the amber lamp is on. s
Please be advised that stainless steel can and will rust if not regularly
cleaned and properly maintained. It is recommended that the bath be
cleaned at least on a monthly basis for moderate to heavy-use installations.
Follow these steps to empty and clean the unit after using it.
Note While cleaning the chamber, be careful not to bend the temperature
control capillary tube which is located along the bottom of the bath
chamber. s
1. Turn off power, unplug the unit, and allow to cool completely.
2. If you have a water bath of 2 or 5 liter capacity, pour the liquid from
the unit into an appropriate disposal container.
3. If you have a larger size water bath, allow the unit to cool and pump
or siphon to empty the unit into an appropriate disposal container.
4. Remove the diffuser tray from the bottom of the bath chamber.
5. Clean the inside of the water bath with mild detergent (such as Joy
dishwashing detergent) and warm water. Do not scrub any surface
with steel wool. (Steel wool leaves small metal particles behind that
will rust, causing the pan to look rusty). In instances where a heavy
coating of residue has accumulated inside the tank or where there is
evidence that corrosion is beginning, the use of a stainless steel cleaner
(such as 3M Stainless Steel Cleaner and Polish) is recommended.
6. Rinse and wipe all tank surfaces with distilled water. The bath is now
ready for use.
Note If Alarm Light comes on during normal cycling, turn backup
controller knob slightly clockwise and monitor. (If backup and digital
controls overlap, the bath will have a wider temperature control band.) s
Isotemp Economy Water Bath 5-3Fisher Scientific
Section 5
Operation
Emptying and
Cleaning the Unit
Procedure 2
(continued))