
FormaModel 3950 Series ______________________________Installation and Start-up
1-10
i. Connecting the CO2Gas Supply
High concentrations of CO2gas can cause asphyxiation.
OSHA Standards specify that employee exposure to carbon dioxide in any 8-hour shift of
a 40-hour work week shall not exceed the 8-hour time weighted average of 5000 PPM (0.5%
CO2). The short-term exposure limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon
dioxide monitors are recommended for confined areas where concentrations of carbon
dioxide gas can accumulate.
This incubator is designed for CO2gas only. Connecting a flammable or toxic
gas could result in a hazardous condition.
Gases other than CO2should not be connected to this equipment. CO2gas cylinders have
a UN1016 label on the cylinder and are equipped with a CGA 320 outlet valve. Check the
gas cylinder for the proper identification labels. The CO2gas supply being connected to the
incubator should be industrial grade, 99.5% pure. Do not use CO2gas cylinders equipped
with siphon tubes. A siphon tube is used to extract liquid CO2from the cylinder, which can
damage the pressure regulator. Consult with your gas supplier to ensure that the CO2
cylinder does not contain a siphon tube. Gas cylinders should also be secured to a wall or
other stationary object to prevent them from tipping.
A two-stage CO2pressure regulator is required to be installed on the outlet valve of the
gas cylinder. Input pressure to the incubator must be maintained at 15 psig (103.4 kPa) for
proper performance of the CO2control system. A single stage CO2pressure regulator will
not maintain 15 psig (103.4 kPa) to the incubator as the pressure in the CO2cylinder
decreases; therefore, a two-stage regulator is recommended.
If higher purity CO2is desired inside the incubator (greater than 99.5% pure), the pressure
regulator should be constructed with a stainless steel diaphragm, along with specifying the
purity of the CO2from the gas supplier. Follow the manufacturer’s instructions to ensure
proper and safe installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed in
accordance with the codes and regulations that are applicable in your area.
The CO2gas supply being connected should be industrial grade 99.5% pure and should not
contain siphon tubes. Install a two-stage pressure regulator at the cylinder outlet. The high
pressure gauge at the tank should have 0-2000 psig range. The low pressure gauge, at the
incubator inlet, should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).
Each tank should be connected to one incubator only.