GE Frac-920 User manual

Fraction Collector Frac-920 and
Frac-950
Operating Instructions
Original instructions

Table of Contents
31 Introduction ..........................................................................................................
41.1 Important user information .............................................................................................................
51.2 Regulatory information ......................................................................................................................
81.3 Instrument ...............................................................................................................................................
91.3.1 Fraction Collector Frac-920 .......................................................................................................
111.3.2 Fraction Collector Frac-950 .......................................................................................................
121.4 Control software ....................................................................................................................................
121.5 User documentation ...........................................................................................................................
142 Safety instructions ...............................................................................................
142.1 Safety precautions ...............................................................................................................................
192.2 Labels .........................................................................................................................................................
212.3 Emergency procedures ......................................................................................................................
222.4 Recycling information .........................................................................................................................
232.5 Declaration of Hazardous Substances (DoHS) ........................................................................
253 Installation ............................................................................................................
253.1 Site requirements ..................................................................................................................................
253.2 Transport ..................................................................................................................................................
263.3 Unpacking ................................................................................................................................................
263.4 Assembly ..................................................................................................................................................
263.5 Connections ............................................................................................................................................
273.6 Spare parts and accessories ...........................................................................................................
284 Operation ..............................................................................................................
284.1 Fraction Collector Frac-920 stand alone ...................................................................................
374.2 Fraction Collector Frac-920 connected to an ÄKTA instrument .....................................
394.3 Fraction Collector Frac-950 .............................................................................................................
475 Maintenance .........................................................................................................
475.1 General ......................................................................................................................................................
475.2 Cleaning ....................................................................................................................................................
485.3 Changing capillaries ............................................................................................................................
485.4 Changing waste tubing ......................................................................................................................
496 Troubleshooting ...................................................................................................
496.1 Faults and actions ................................................................................................................................
517 Reference information ........................................................................................
517.1 Specifications .........................................................................................................................................
527.2 Health and Safety Declaration Form ...........................................................................................
547.3 Ordering information ..........................................................................................................................
2 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
Table of Contents

1 Introduction
Purpose of the Operating
Instructions
The Operating Instructions provide you with the instructions needed to handle Fraction
Collector Frac-920 and Frac-950 in a safe way.
Prerequisites
In order to operate Fraction Collector Frac-920 and Frac-950 as is intended, the following
pre-requisites must be fulfilled:
•The user should have a general understanding of how a PC and the Microsoft®
Windows®operating system works. (if a computer is used)
•The user must understand the concepts of liquid chromatography.
•The user must read and understand the Safety Instructions in this manual.
•Fraction Collector Frac-920 and Frac-950 and software should be installed, configured
and calibrated according to these Operating Instructions.
About this chapter
This chapter contains important user information, a description of the intended use of
Fraction Collector Frac-920 and Frac-950, regulatory information, list of associated
documentation, definitions of safety notices and so on.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 3
1 Introduction

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use
Fraction Collector Frac-920 and Fraction Collector Frac-950 are automated fraction
collectors for use in ÄKTA™ chromatography systems. Fraction Collector Frac-920 can
also be used as a stand-alone unit.
Fraction Collector Frac-920 and Frac-950 are intended for research use only, and shall
not be used in any clinical procedures, or for diagnostic purposes.
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the
safe use of the product. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
4 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
1 Introduction
1.1 Important user information

Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (e.g., Power switch).
1.2 Regulatory information
In this section
This section describes the directives and standards that are fulfilled by Fraction Collector
Frac-920 and Frac-950.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 5
1 Introduction
1.1 Important user information

Manufacturing information
The table below summarizes the required manufacturing information. For further infor-
mation, see the EU Declaration of Conformity (DoC) document.
ContentRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
Conformity with EU Directives
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2004/108/EC
Low Voltage Directive (LVD)2006/95/EC
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used as a stand-alone unit, or
•connected to other products recommended or described in the user documentation,
and
•used in the same state as it was delivered from GE Healthcare, except for alterations
described in the user documentation.
6 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
1 Introduction
1.2 Regulatory information

International standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
EN standard is harmonized
with EU directive
2006/95/EC
Safety requirements for electrical
equipment for measurement,
control, and laboratory use
EN/IEC 61010-1,
UL 61010-1,
CAN/CSA-C22.2
No. 61010-1
EN standard is harmonized
with EU directive
2004/108/EC
Electrical equipment for measure-
ment, control and laboratory use
- EMC requirements
EN 61326-1
EN ISO standard is harmo-
nized with EU directive
2006/42/EC
Safety of machinery. General
principles for design. Risk assess-
ment and risk reduction.
EN ISO 12100
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE Healthcare could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class B digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference in a residential installation. This equip-
ment generates, uses and can radiate radio frequency energy and, if not installed and
used in accordance with the instructions, may cause harmful interference to radio
communications. However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the equipment off and on, the
user is encouraged to try to correct the interference by one or more of the following
measures:
•Reorient or relocate the receiving antenna.
•Increase the separation between the equipment and receiver.
•Connect the equipment into an outlet on a circuit different from that to which the
receiver is connected.
•Consult the dealer or an experienced radio/TV technician for help.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 7
1 Introduction
1.2 Regulatory information

Regulatory compliance of
connected equipment
Any equipment connected to Fraction Collector Frac-920 and Frac-950 should meet the
safety requirements of EN 61010-1/IEC 61010-1, or relevant harmonized standards.
Within the EU, connected equipment must be CE marked.
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Administration on the Control of Pollution Caused by Electronic
Information Products, China Restriction of Hazardous Sub-
stances (RoHS)
ACPEIP
Registration, Evaluation, Authorization and restriction of
CHemicals (REACH)
Regulation (EC)
No 1907/2006
1.3 Instrument
Fraction Collector Frac-920 and Frac-950 are automated fraction collectors for use in
ÄKTA chromatography systems. Fraction Collector Frac-920 can also be used as a stand-
alone unit.
8 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
1 Introduction
1.2 Regulatory information

1.3.1 Fraction Collector Frac-920
Main parts
1
2
3
4
5
6
7
8
Table 1.1: The main parts of Fraction Collector Frac-920.
DescriptionPart
Delivery arm1
Lock knob2
Control buttons3
Control buttons4
Collection tubes5
Tube rack6
Programming buttons7
Menu display8
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 9
1 Introduction
1.3 Instrument
1.3.1 Fraction Collector Frac-920

Electrical and communication
connections
DescriptionPart
Drop sensor1
Valve2
Auxillary equipment3
Remote/digital I/O4
UniNet-15
Power inlet6
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1 Introduction
1.3 Instrument
1.3.1 Fraction Collector Frac-920

1.3.2 Fraction Collector Frac-950
Main parts
FRAC 950
FRAC950
FRAC950
FRAC95 0
FRAC950
Standardmode
Standardm
ode
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
Table 1.2: The main part of Fraction Collector Frac-950
DescriptionPart
Delivery arm1
Safety bar2
Collection tubes3
Tube rack4
Indicators5
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 11
1 Introduction
1.3 Instrument
1.3.2 Fraction Collector Frac-950

Electrical and communication
connections
Voltage Frequency Power max.
100-120 / 50-60 Hz 300 VA
220-240 V~ Mains
RemoteDropSync
UniNet 3 UniNet 1
1
2 3
4
5
DescriptionPart
UniNet-3 connectors1
DropSync connector2
Power inlet3
Power switch4
UniNet-1 connector5
1.4 Control software
UNICORN™control software
UNICORN is a complete software for control and supervision of Fraction Collector Frac-920
and Frac-950. The software runs under Microsoft®Windows operating system.
For more information about UNICORN control system, see the UNICORN user manuals
supplied.
1.5 User documentation
In addition to these Operating Instructions, the documentation package supplied with
Fraction Collector Frac-920 and Frac-950 also includes product documentation binders
containing detailed specifications and traceability documents.
The most important documents in the document package with regard to technical aspects
of Fraction Collector Frac-920 and Frac-950 are:
12 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
1 Introduction
1.3 Instrument
1.3.2 Fraction Collector Frac-950

System-specific documentation
ContentUser documentation
All instructions needed to operate the instrument
in a safe way, including brief system description,
installation, and maintenance.
Fraction Collector Frac-920 and
Frac-950 Operating Instructions
Detailed system description. Comprehensive user
instructions, method creation, operation, ad-
vanced maintenance and troubleshooting.
Fraction Collector Frac-920 and
Frac-950 User Manuals
Document whereby the manufacturer ensures
that the product satisfies and is in conformity with
the essential requirements of the applicable direc-
tives.
EU Declaration of Conformity for
Fraction Collector Frac-920 and
Frac-950
Software documentation
Together with each system, the following software documentation is supplied providing
additional information that applies to Fraction Collector Frac-920 and Frac-950, indepen-
dent of the specific configuration:
Purpose/ContentsDocument
•The manuals contain detailed instructions on
how to administer UNICORN, work with methods,
perform runs and evaluate results.
•The Online help contains dialog descriptions for
UNICORN. The Online help is accessed from the
Help menu.
UNICORN™ manual package
Component documentation
Documentation for components produced both by GE Healthcare and by a third-party
are, if existent, also included in the document package.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 13
1 Introduction
1.5 User documentation

2 Safety instructions
About this chapter
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures, power failure and recycling of Fraction Collector Frac-920 and
Frac-950.
2.1 Safety precautions
Introduction
Fraction Collector Frac-920 and Frac-950 are powered by mains voltage and handles
liquids that may be hazardous. Before installing, operating or maintaining the system,
you must be aware of the hazards described in this manual. Follow the instructions
provided to avoid personal injury or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Using flammable liquids
•Personal protection
•Installing and moving the instrument
•System operation
•Maintenance
General precautions
Always follow these General precautions to avoid injury when using Fraction Collector
Frac-920 and Frac-950.
WARNING
Do not operate Fraction Collector Frac-920 and Frac-950 in any
other way than described in the Fraction Collector Frac-920 and
Frac-950 Operating Instructions.
14 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
2 Safety instructions

WARNING
Operation and user maintenance of the Fraction Collector Frac-920
and Frac-950 should be performed by properly trained personnel
only.
WARNING
Do not use any accessories not supplied or recommended by GE
Healthcare.
WARNING
Do not use the Fraction Collector Frac-920 and Frac-950 if it is not
working properly, nor if it has suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
WARNING
Operation and user maintenance of Fraction Collector Frac-920
and Frac-950 should be performed by properly trained personnel
only.
WARNING
Do not use the Fraction Collector Frac-950 if the safety bar is bro-
ken.
CAUTION
Waste tubes and containers must be secured and sealed to prevent
accidental spillage.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 15
2 Safety instructions
2.1 Safety precautions

CAUTION
Make sure waste container is dimensioned for maximum possible
volume when the equipment is left unattended.
NOTICE
Avoid condensation by letting the unit equilibrate to ambient tem-
perature.
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Personal protection
WARNING
Always use appropriate personal protective equipment during
operation and maintenance of Fraction Collector Frac-920 and
Frac-950.
WARNING
When using hazardous chemical and biological agents, take all
suitable protective measures, such as wearing protective glasses
and gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of Fraction
Collector Frac-920 and Frac-950.
16 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
2 Safety instructions
2.1 Safety precautions

WARNING
Spread of biological agents. The operator has to take all necessary
actions to avoid spreading hazardous biological agents in the
vicinity of the instrument. The facility should comply with the na-
tional code of practice for biosafety.
Installing and moving the
instrument
WARNING
Supply voltage. Make sure that the supply voltage at the wall
outlet corresponds to the marking on the equipment, before con-
necting the power cord.
WARNING
Fraction Collector Frac-920 and Frac-950 must always be connect-
ed to a grounded power outlet.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE Healthcare.
WARNING
Access to power switch and power cord with plug. Do not block
access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 17
2 Safety instructions
2.1 Safety precautions

NOTICE
Any computer used with the equipment shall comply with IEC 60950
and be installed and used according to the manufacturer's instruc-
tions.
System operation
WARNING
Hazardous chemicals during run. When using hazardous chemi-
cals, run System CIP and Column CIP to flush the entire system
tubing with distilled water, before service and maintenance.
WARNING
Hazardous biological agents during run. When using hazardous
biological agents, run System CIP and Column CIP to flush the
entire system tubing with bacteriostatic solution (e.g. NaOH) fol-
lowed by a neutral buffer and finally distilled water, before service
and maintenance.
CAUTION
Pinch hazard. Do not have any part of your body within the unit
base area when Fraction Collector Frac-950 is switched on. An
automatic calibration process starts when Fraction Collector
Frac-950 is connected to UNICORN. During calibration, the dis-
penser arm moves rapidly. Several beeps are heard before the
calibration procedure starts.
CAUTION
Be sure to fold down the safety bar whenever the rack holder is
operated by hand. This blocks the rack holder from accidentally
moving while the rack is moved by hand or replaced. (Fraction
Collector Frac-950 only)
18 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
2 Safety instructions
2.1 Safety precautions

Maintenance
WARNING
Electrical shock hazard. All repairs should be done by service
personnel authorized by GE Healthcare. Do not open any covers
or replace parts unless specifically stated in the user documenta-
tion.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
2.2 Labels
In this section
This section describes the instrument labels and labels concerning hazardous substances
that are attached to the Fraction Collector Frac-920 and Frac-950 instrument. For infor-
mation about marking of the computer equipment, refer to the manufacturer’s instruc-
tions.
Labels on the instrument
The illustration below shows an example of the identification label that is attached to
Fraction Collector Frac-920 and Frac-950.
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 19
2 Safety instructions
2.1 Safety precautions

Symbols used in instrument
labels
MeaningLabel
Warning! Read the user documentation before using the equipment.
Do not open any covers or replace parts unless specifically stated in
the user documentation.
The equipment complies with the requirements for electromagnetic
compliance (EMC) in Australia and New Zealand.
The equipment complies with applicable European directives.
This symbol indicates that Fraction Collector Frac-920 and Frac-950
has been certified by a Nationally Recognized Testing Laboratory
(NRTL). NRTL means an organization, which is recognized by the US
Occupational Safety and Health Administration (OSHA) as meeting
the legal requirements of Title 29 of the Code of Federal Regulations
(29 CFR), Part 1910.7.
Labels concerning hazardous
substances
MeaningLabel
This symbol indicates that the waste of electrical and electronic equip-
ment must not be disposed as unsorted municipal waste and must be
collected separately. Please contact an authorized representative of the
manufacturer for information concerning the decommissioning of
equipment.
20 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
2 Safety instructions
2.2 Labels