GE SEMIPHOR TRANSPHOR Series User manual

SEMIPHOR TRANSPHOR series
Operating Instructions
Original instructions

Table of Contents
31 Introduction ..........................................................................................................
41.1 About this manual ................................................................................................................................
61.2 Important user information .............................................................................................................
81.3 Regulatory information ......................................................................................................................
91.3.1 EU directives .....................................................................................................................................
101.3.2 Eurasian Customs Union ............................................................................................................
111.3.3 Regulations for USA and Canada ...........................................................................................
131.3.4 Other regulations and standards ............................................................................................
142 Safety instructions ...............................................................................................
152.1 Safety precautions ...............................................................................................................................
212.2 Labels .........................................................................................................................................................
232.3 Emergency procedures ......................................................................................................................
252.4 Recycling information .........................................................................................................................
262.5 Declaration of Hazardous Substances (DoHS) ........................................................................
283 System description ..............................................................................................
293.1 Instrument overview ...........................................................................................................................
313.2 Detailed description ............................................................................................................................
344 Installation ............................................................................................................
355 Operation ..............................................................................................................
375.1 Assemble transfer stack ....................................................................................................................
45
5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR
TRANSPHOR UNIT .................................................................................................................................
47
5.3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR
TRANSPHOR UNIT .................................................................................................................................
505.4 Changing parameters during the transfer ...............................................................................
525.5 After the transfer is complete .........................................................................................................
546 Maintenance .........................................................................................................
567 Troubleshooting ...................................................................................................
618 Reference information ........................................................................................
628.1 Specifications .........................................................................................................................................
648.2 Ordering information ..........................................................................................................................
658.3 Health and Safety Declaration Forms .........................................................................................
67Index .......................................................................................................................
2 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, and intended use of the SEMIPHOR TRANSPHOR UNIT series.
In this chapter
See pageSection
41.1 About this manual
61.2 Important user information
81.3 Regulatory information
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 3
1 Introduction

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the SEMIPHOR TRANSPHOR UNIT series, including
SEMIPHOR TRANSPHOR UNIT, LARGE SEMIPHOR TRANSPHOR UNIT, PWR LARGE SEMIPHOR
TRANSPHOR UNIT, and PWR SEMIPHOR TRANSPHOR UNIT. The illustration below shows
the SEMIPHOR TRANSPHOR UNIT or LARGE SEMIPHOR TRANSPHOR UNIT.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not reflect
the actual system delivered.
Typographical conventions
In this document all the LARGE SEMIPHOR TRANSPHOR UNIT, SEMIPHOR TRANSPHOR
UNIT, PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT
instruments are refered to as SEMIPHOR TRANSPHOR UNIT series.
4 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
1 Introduction
1.1 About this manual

Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Power).
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 5
1 Introduction
1.1 About this manual

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the product
SEMIPHOR TRANSPHOR UNIT series are semi-dry blotters intended primarily for blotting
of proteins at laboratory-scale. The system can be used for a variety of research purposes
to fulfill the needs of the users in academia and in life sciences industry. PWR LARGE
SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT have a built-in
power supply. LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT
require an external power supply.
SEMIPHOR TRANSPHOR UNIT series shall not be used in any clinical procedures, or for
diagnostic purposes.
Prerequisites
In order to operate the SEMIPHOR TRANSPHOR UNIT series in the way it is intended:
•The user must have a general understanding of electrophoresis and blotting tech-
niques.
•The user must read and understand the Safety Instructions chapter in the Operating
Instructions.
•SEMIPHOR TRANSPHOR UNIT series must be installed in accordance with the instruc-
tions in the Operating Instructions.
6 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
1 Introduction
1.2 Important user information

Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 7
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section lists the regulations and standards that apply to the SEMIPHOR TRANSPHOR
UNIT series.
Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
91.3.1 EU directives
101.3.2 Eurasian Customs Union
111.3.3 Regulations for USA and Canada
131.3.4 Other regulations and standards
8 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
1 Introduction
1.3 Regulatory information

1.3.1 EU directives
Introduction
This section describes the EU Directives that apply to SEMIPHOR TRANSPHOR UNIT series.
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Electromagnetic Compatibility (EMC) Directive2014/30/EU
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used according to the Operating Instructions or user manuals, and
•used in the same state as it was delivered from GE Healthcare, except for alterations
described in the Operating Instructions or user manuals.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 9
1 Introduction
1.3 Regulatory information
1.3.1 EU directives

1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/or company for obtain-
ing information about importer GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: [email protected]
10 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
1 Introduction
1.3 Regulatory information
1.3.2 Eurasian Customs Union

1.3.3 Regulations for USA and Canada
Introduction
This section describes the regulations that apply to SEMIPHOR TRANSPHOR UNIT series
in the USA and Canada.
NRTL certification
This symbol indicates that the product has been certified by Intertek, which is a US Oc-
cupational Safety and Health Administration Nationally Recognized Testing Laboratory
(NRTL).
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE Healthcare could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class B digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference in a residential installation. This equip-
ment generates, uses and can radiate radio frequency energy and, if not installed and
used in accordance with the instructions, may cause harmful interference to radio
communications. However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the equipment off and on, the
user is encouraged to try to correct the interference by one or more of the following
measures:
•Reorient or relocate the receiving antenna.
•Increase the separation between the equipment and receiver.
•Connect the equipment into an outlet on a circuit different from that to which the
receiver is connected.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 11
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

•Consult the dealer or an experienced radio/TV technician for help.
12 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

1.3.4 Other regulations and standards
Introduction
This section describes the standards that apply to the SEMIPHOR TRANSPHOR UNIT
series.
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Management Methods for the Restriction of the Use of Haz-
ardous Substances in Electrical and Electronic Products.
China RoHS
Standards, applied to this
product
Standard requirements fulfilled by this product are summarized in the table below.
DescriptionStandard
Safety requirements for electrical equipment for mea-
surement, control, and laboratory use - Part 1: General
requirements.
IEC/EN 61010-1, UL
61010-1, CAN/CSA-C22.2
No. 61010-1
Electrical Equipment for Measurement, Control, and
Laboratory Use-EMC requirements-Part 1: General re-
quirements
IEC/EN 61326-1
Technical documentation for the assessment of electrical
and electronic products with respect to the restriction
of hazardous substances
EN 50581
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 13
1 Introduction
1.3 Regulatory information
1.3.4 Other regulations and standards

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures, and
provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
This chapter contains the following sections:
See pageSection
152.1 Safety precautions
212.2 Labels
232.3 Emergency procedures
252.4 Recycling information
262.5 Declaration of Hazardous Substances (DoHS)
14 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2 Safety instructions

2.1 Safety precautions
Introduction
SEMIPHOR TRANSPHOR UNIT series handle materials that are considered hazardous
material and LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT are
powered by an external power supply. Before installing, operating or maintaining the
system, you must be aware of the hazards described in this manual.
Follow the instructions provided to avoid injury to the operator or other personnel,
to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the instrument
•Power supply
•System operation
•Maintenance
Always follow the instructions below to avoid injury when using the SEMIPHOR
TRANSPHOR UNIT series.
General precautions
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 15
2 Safety instructions
2.1 Safety precautions

WARNING
Do not operate the SEMIPHOR TRANSPHOR UNIT series in any other
way than described in the SEMIPHOR TRANSPHOR UNIT series Op-
erating Instructions.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by GE Healthcare may be used for
maintaining or servicing the product.
WARNING
This instrument is designed for indoor laboratory use only.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
Healthcare representative for replacements.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
16 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2 Safety instructions
2.1 Safety precautions

WARNING
Do not immerse the unit in water.
CAUTION
Never autoclave any component of the electrophoresis unit or
casting kit.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using haz-
ardous chemical and biological agents, take all suitable protective
measures, such as wearing protective clothing, glasses and gloves
resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of this product.
WARNING
Spread of biological agents. The operator must take all necessary
actions to avoid spreading hazardous biological agents. The facility
must comply with the national code of practice for biosafety.
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 17
2 Safety instructions
2.1 Safety precautions

Installing and moving the
instrument
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE Healthcare.
System operation
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
18 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2 Safety instructions
2.1 Safety precautions

WARNING
The electric components in the transfer unit base must not become
wet.
WARNING
Supply voltage. Before connecting the power cord, make sure that
the supply voltage at the wall outlet corresponds to the marking
on the instrument.
WARNING
Be careful when cutting the masks. The blades are sharp and may
cause cuts.
CAUTION
Devices must only be operated with a certified power supply capa-
ble of limiting the voltage and current to specified ratings.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
SEMIPHOR TRANSPHOR UNIT series is properly decontaminated
and sanitized before maintenance or service.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 19
2 Safety instructions
2.1 Safety precautions

WARNING
Decommissioning. Decontaminate the equipment before decom-
missioning to make sure that hazardous residues are removed.
20 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2 Safety instructions
2.1 Safety precautions