GE SE 250 Mighty Small II User manual

SE 250/260 Mighty Small II
Operating Instructions
Original instructions

Table of Contents
31 Introduction ..........................................................................................................
41.1 About this manual ................................................................................................................................
51.2 Important user information .............................................................................................................
71.3 Regulatory information ......................................................................................................................
81.3.1 EU directives .....................................................................................................................................
91.3.2 Eurasian Customs Union ............................................................................................................
101.3.3 Regulations for USA and Canada ...........................................................................................
111.3.4 Other regulations and standards ............................................................................................
122 Safety information ...............................................................................................
132.1 Safety precautions ...............................................................................................................................
202.2 Labels .........................................................................................................................................................
222.3 Emergency procedures ......................................................................................................................
232.4 Recycling information .........................................................................................................................
242.5 Declaration of Hazardous Substances (DoHS) ........................................................................
263 System description ..............................................................................................
284 Installation ............................................................................................................
295 Operation ..............................................................................................................
315.1 Preparations ............................................................................................................................................
345.2 Assembly ..................................................................................................................................................
405.3 Electrophoresis run ..............................................................................................................................
426 Maintenance .........................................................................................................
447 Troubleshooting ...................................................................................................
478 Reference information ........................................................................................
488.1 Specifications .........................................................................................................................................
498.2 Ordering information ..........................................................................................................................
508.3 Health and Safety Declaration Forms .........................................................................................
52Index .......................................................................................................................
2 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, and intended use of the SE 250/260 Mighty Small II.
In this chapter
See pageSection
41.1 About this manual
51.2 Important user information
71.3 Regulatory information
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 3
1 Introduction

1.1 About this manual
Purpose of this manual
The Operating instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions covers the SE 250/260 Mighty Small II. The illustration below
shows the SE 250/260 Mighty Small II.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not reflect
the actual system delivered.
4 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.1 About this manual

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating instruction before installing, operating or
maintaining the product.
Always keep the Operating instruction at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the product
The SE 250/260 Mighty Small II small format vertical slab gel unit is intended for rapid
electrophoresis of protein or nucleic acid samples. Most samples can be run in 45 minutes,
and only a minimal amount of sample is required.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm gel sandwiches.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm or 10 × 10.5 cm
gel sandwiches. In both SE 250/260 Mighty Small II units, the upper buffer chamber is
formed when the notched side of a gel sandwich is sealed against the silicone rubber
gasket.
The upper buffer chamber core serves as a heat exchanger if cooling is required. The
core is hollow and equipped with ports on either side for coolant circulation.
Prerequisites
In order to operate the SE 250/260 Mighty Small II in the way it is intended:
•The user must have a general understanding of gel electrophoresis.
•The user must read and understand the Safety Instructions chapter in the Operating
Instructions.
•The SE 250/260 Mighty Small II must be installed in accordance with the instructions
in the Operating Instructions.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 5
1 Introduction
1.2 Important user information

Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
6 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section lists the regulations and standards that apply to the SE 250/260 Mighty
Small II.
Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
81.3.1 EU directives
91.3.2 Eurasian Customs Union
101.3.3 Regulations for USA and Canada
111.3.4 Other regulations and standards
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 7
1 Introduction
1.3 Regulatory information

1.3.1 EU directives
Introduction
This section describes the EU Directives that apply to SE 250/260 Mighty Small II.
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used according to the Operating Instructions or user manuals, and
•used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.
8 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.3 Regulatory information
1.3.1 EU directives

1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/or company for obtain-
ing information about importer GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: [email protected]
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 9
1 Introduction
1.3 Regulatory information
1.3.2 Eurasian Customs Union

1.3.3 Regulations for USA and Canada
Introduction
This section describes the regulations that apply to SE 250/260 Mighty Small II in the
USA and Canada.
NRTL certification
This symbol indicates that the product has been certified by Intertek, which is a US Oc-
cupational Safety and Health Administration Nationally Recognized Testing Laboratory
(NRTL).
10 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

1.3.4 Other regulations and standards
Introduction
This section describes the standards that apply to the SE 250/260 Mighty Small II.
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Management Methods for the Restriction of the Use of Haz-
ardous Substances in Electrical and Electronic Products.
China RoHS
Standards, applied to this
product
Standard requirements fulfilled by this product are summarized in the table below.
DescriptionStandard
Safety requirements for electrical equipment for mea-
surement, control, and laboratory use - Part 1: General
requirements.
IEC/EN 61010-1, UL
61010-1, CAN/CSA-C22.2
No. 61010-1
Technical documentation for the assessment of electrical
and electronic products with respect to the restriction
of hazardous substances
EN 50581
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 11
1 Introduction
1.3 Regulatory information
1.3.4 Other regulations and standards

2 Safety information
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures, and
provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
See pageSection
132.1 Safety precautions
202.2 Labels
222.3 Emergency procedures
232.4 Recycling information
242.5 Declaration of Hazardous Substances (DoHS)
12 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information

2.1 Safety precautions
Introduction
The SE 250/260 Mighty Small II is powered by an external power supply. Before installing,
operating or maintaining the system, you must be aware of the hazards described in
this manual.
Follow the instructions provided to avoid injury to the operator or other personnel,
to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the product
•Power supply
•System operation
•Maintenance
Always follow the instructions below to avoid injury when using the SE 250/260 Mighty
Small II.
General precautions
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 13
2 Safety information
2.1 Safety precautions

WARNING
Do not operate the SE 250/260 Mighty Small II in any other way
than described in the SE 250/260 Mighty Small II Operating Instruc-
tions.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
representative for replacements.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in
the heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part
of the instrument. Organic solvents will cause irreparable damage
to the instrument.
14 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions

Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using haz-
ardous chemical and biological agents, take all suitable protective
measures, such as wearing protective clothing, glasses and gloves
resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of this product.
WARNING
Spread of biological agents. The operator must take all necessary
actions to avoid spreading hazardous biological agents. The facility
must comply with the national code of practice for biosafety.
CAUTION
Handle the glass components with care! Wear appropriate personal
protective equipment (PPE).
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 15
2 Safety information
2.1 Safety precautions

Installing and moving the
product
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
The electrophoresis unit is heavy, especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
16 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions

WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high volt-
age power supply must never be switched on unless the safety lid
is on the electrophoresis unit.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
WARNING
Always disconnect the high voltage leads from the power supply
before removing the lid from the unit.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 17
2 Safety information
2.1 Safety precautions

CAUTION
Circulate coolant through the heat exchanger to minimize heating.
Overheating will cause irreparable damage to the unit! Do not
connect the heat exchanger to a water tap or any coolant source
where the water pressure is unregulated.
CAUTION
Do not operate with buffer temperatures above the maximum
specified technical specifications. Overheating will cause irreparable
damage to the unit!
CAUTION
Devices must only be operated with a certified power supply capa-
ble of limiting the voltage and current to specified ratings.
NOTICE
After initial monitoring, do not leave the unit unattended for more
than 45 min before checking the progress of the bands and the
buffer level.
NOTICE
If running only one gel, block off the unused part of the core with
a glass plate. Do not fill this side with buffer.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
SE 250/260 Mighty Small II is properly decontaminated and sani-
tized before maintenance or service.
18 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions

WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by GE may be used for maintaining or
servicing the product.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
WARNING
Decommissioning. Decontaminate the equipment before decom-
missioning to make sure that hazardous residues are removed.
NOTICE
Cleaning. Keep the exterior of the instrument dry and clean. Wipe
regularly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let the instrument dry completely before use.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 19
2 Safety information
2.1 Safety precautions

2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
Description of symbols on the
system label
The table below describes the various symbols that may be found on the system label.
MeaningLabel
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
This symbol indicates that the waste of electrical and electronic
equipment must not be disposed as unsorted municipal waste and
must be collected separately. Please contact an authorized repre-
sentative of the manufacturer for information concerning the de-
commissioning of equipment.
This symbol indicates that the product does not contain toxic or
hazardous materials in excess of the limits established by the Chi-
nese standard GB/T 26572 Requirements of concentration limits for
certain hazardous substances in electrical and electronic products,
and can be recycled after being discarded, and should not be casu-
ally discarded.
The system complies with applicable European directives.
Eurasian Conformity mark: the single conformity mark indicates
that the product is approved for circulation on the markets of the
member states of the Eurasian Customs Union.
20 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.2 Labels