Gilson 333 User manual

User's Guide
333/334 HPLC Pumps

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333/334 HPLC PUMPS | USER’S GUIDE
TABLE OF CONTENTS
SAFETY | 7
Electronic and Hazard Symbols | 8
Safety Notices | 8
Lifting | 9
Voltage | 9
Solvents | 9
Replacement Parts | 9
CHAPTER 1 | INTRODUCTION | 11
Description | 12
Unpacking | 15
Technical Specifications | 16
Customer Service | 18
CHAPTER 2 | INSTALLATION | 19
Electrical Connections | 20
Cabling for TRILUTION LC Control | 24
Cabling for Control from 333 Pump | 24
Twin Pump Systems | 25
Positioning the Pumps | 26
Hydraulic Connections | 28
CHAPTER 3 | OPERATION | 33
Front Panel and Startup | 34
Priming | 34
Pump Operating Parameters | 37

TABLEOFCONTENTS |333/334 HPLC PUMPS
TABLE OF CONTENTS
CHAPTER 4 | MAINTENANCE | 39
Maintenance Overview | 40
Cleaning | 40
Pump Head | 41
Piston Seals | 45
Piston and Bellows | 46
Piston Rinsing Chamber | 47
Reassemble Pump Head | 48
Check Valves | 49
Filters | 51
Maintenance Procedures | 52
Resetting Maintenance Logs | 52
Fuse Replacement | 52
CHAPTER 5 | TROUBLESHOOTING | 53
Error LED (333 Pump) | 54
Error Messages (333 Pump) | 55
Troubleshooting | 56
Repair and Return Policies | 58
APPENDIX A | REPLACEMENT PARTS | 59
333/334 HPLC Pumps | 59
Hydraulic | 59
Pump Head | 61
Electrical | 61
Miscellaneous (Tools, Power Cords, Cables, etc.) | 62
Service Part | 62
APPENDIX B | COLUMN HOLDER | 63
Installation | 63
Part Numbers | 64

333/334 HPLC PUMPS | USER’S GUIDE
TABLE OF CONTENTS
APPENDIX C | INJECTOR | 65
Injection Valve Holder | 66
Connection to Injector | 66
Part Numbers | 66
APPENDIX D | FRONT PANEL CONTROL | 67
Using the Control Panel | 68
Electrical Connections | 69
Basic Control Configurations | 70
Hardware Configuration | 72
Safety Functions | 74
Electrical Contacts | 76
Manual Operations | 78
Entering Method Programs | 80
Method Program Worksheet | 84
File Management | 86
Good Laboratory Practice (GLP Functions) | 88
Running a Method Program | 92
APPENDIX E | REFERENCE INFORMATION | 93
Liquid Contact Materials | 93
Solvent Miscibility | 95

Chapter1|SaFetY
The instrument is intended to be used in a laboratory by trained technical personnel. For safe
and correct use, both operating and service personnel should follow the instructions contained
in this guide when installing, cleaning, and maintaining the instrument.
The following safety precautions must be observed during all phases of operation, service, and
repair of the instrument. Failure to comply with these precautions or with specific warnings
elsewhere in the user’s guide violates safety standards of design, manufacture, and intended
use of the instrument. Gilson assumes no liability for the customer’s failure to comply with
these requirements.
The instrument has been certified to safety standards required in Canada, Europe, and the
United States. Refer to the rear panel label on the instrument or the Declaration of Conformity
document for the current standards to which the instrument has been found compliant.
IN thIS Chapter
●Electronic and Hazard Symbols | 8
●Safety Notices | 8
●Lifting | 9
●Voltage | 9
●Solvents | 9
●Replacement Parts | 9
7
333/334 HPLC PUMPS | USER’S GUIDE

8SAFETY |333/334 HPLC PUMPS
SAFETY
Electronic and Hazard Symbols
The following electronic and hazard symbols may appear on the instrument:
Symbol Explanation
~Alternating current
Direct current
Protective conductor terminal
|Electrical power ON
OElectrical power OFF
Caution, risk of electric shock
Caution
Fuse
Safety Notices
The following safety notices may appear in this document:
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, may result in
serious injury
CAUTION
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury
NOTICE
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in
equipment damage

9
333/334 HPLC PUMPS | USER’S GUIDE
SAFETY
Lifting
The instrument exceeds the weight one person can lift safely. Two or more people are required
to lift the instrument safely. Refer to the Technical Specifications for the weight. Always lift the
instrument from the base and follow any unpacking instructions provided with the instrument.
Voltage
Access to the rear panel is necessary. The instrument must be detached from all voltage
sources before service, repair, or exchange of parts. For normal operation, the instrument is to
be grounded through the AC line cord and power supply provided. Failure to do so can result
in a potential shock hazard that could result in serious personal injury.
Use only fuses with the rated current and of the specified type as listed on the rear panel label
on the instrument. The instrument must only be operated with the voltage specified on the rear
panel label of the instrument and with the grounded AC line cord and power supply provided.
Solvents
Observe safe laboratory practices when handling solvents. If working with hazardous solvents
or flammable liquids, ensure that there is proper ventilation and that adequate protection such
as safety glasses, gloves, and protective clothing are used.
If dangerous liquids are used, precautions should be taken to limit potential hazards from leaks
and/or spillage through the use of a non-flammable tray or use of a fume hood, etc.
If there is the potential of explosive gases being developed, a fume hood or other means should
be used to safely manage that risk.
Refer to the Material Safety Data Sheets for the solvents before use.
Replacement Parts
Be sure to use only replacement parts mentioned in the user’s guide. Do not repair the instrument
or change parts not listed in the user’s guide. If it is necessary to replace parts not listed, please
contact your local Gilson representative.

Chapter 1
CHAPTER1|INTRODUCTION
IN THIS CHAPTER
●Description | 12
●Unpacking | 15
●Technical Specifications | 16
●Customer Service | 18
11
333/334 HPLC PUMPS | USER’S GUIDE

Description
The 333/334 HPLC Pumps are reciprocating
piston pumps that provide a preparative
pumping solution accommodating flow rates
from 1 mL/min to 200 mL/min and pressures up
to 3040 psi (210 bar). With additional pumps
in parallel, you can double the flow rate up to
400 mL/min. Individually, a 333 Pump is for
single solvent delivery (isocratic) under high
pressure. When coupled with a 334 Pump,
multi-solvent delivery (binary) is possible.
The 333/334 Pumps are controlled by either
333 Pump software using its control panel
or by TRILUTION® LC Software as part of an
HPLC system.
Hydraulic Components
Open the removable door to view and/or access the hydraulics. Both the 333 Pump and
334 Pump include inlet and outlet tees to direct solvents and reciprocating piston pump
heads to reduce flow pulsation. The 333 Pump has an integrated pressure, purge and mixing
module (PPMM) that functions as a static mixer, a pressure transducer, and a purge valve. The
333 Pump also has an outlet filter to protect columns from contaminants. There is a drip tray at
the bottom of the pump (see Drip Tray on page 30). Solvent bottles can be placed in the tray
at the top of the pump (see Solvent Bottle Tray on page 30).
Figure 2
Hydraulic Connections between the 333/334 HPLC Pumps and the HPLC System (Side-by-Side Configuration)
Figure 1
333/334 HPLC Pumps
12 INTRODUCTION |333/334 HPLC PUMPS
DESCRIPTION

PUMP HEADS
Each pump head has a solvent inlet port, a solvent outlet port, an inlet
port to the rinsing chamber, an outlet port from the rinsing chamber,
and a reciprocating piston.
The piston seal and the bellows are inside the pump head. The solvent
inlet port and the solvent outlet port are fitted with connectors
containing the check valves. All of these items can be serviced by the
user.
The pump heads are pre-mounted directly onto the tops of the driving
mechanisms, which have the same axis as the piston motors, so pump
heads may be changed with relative ease. The pump heads must be
dismounted for routine servicing purposes (for example, changing a
piston seal).
PPMM (333 PUMP ONLY)
The PPMM combines three functions:
●A static mixer to blend up to three solvents at high pressure.
●A built-in transducer that detects the pressure in this part of the hydraulic circuit.
●A purge valve which is used to manually direct the mobile phase towards the outlet filter or
divert to the drain.
OUTLET FILTER (333 PUMP ONLY)
The outlet filter protects the injection valve and the column.
The outlet filter can be replaced by the user.
Figure 3
H3 Pump Head
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333/334 HPLC PUMPS | USER’S GUIDE
DESCRIPTION

Control Panels
FRONT PANEL
The 333 Pump features a front control panel with a display screen, soft keys, and fixed function
keys. The 334 Pump does not have a front control panel and must be controlled by the 333 Pump
or by TRILUTION® LC Software. Both pumps utilize standby panels to control power or indicate
errors.
REAR PANEL
All electrical connections are made on the rear panels. The
rear panel of the 333 Pump houses a main power switch,
14-pin contact output port, 10-pin contact input/output
port, and GSIOC ports. The rear panel of the 334 Pump
houses the main power switch, a GSIOC port, unit ID
selector, and baud rate selector. The pumps have two
ON/OFF switches: one on the power receptacle on the
rear panel and the other on the front panel above the
LEDs.
Figure 7
333 HPLC Pump Rear Panel Diagram
Figure 8
334 HPLC Pump Rear Panel Diagram
Figure 4
333 HPLC Pump Front Control Panel and Standby Panels
!
POWER
ON
ERROR
!
P
OW
ON
ON
ER
R
E
R
O
ON/OFF
!
POWER
ON
REMOTE
ON/OFF
Figure 5
334 HPLC Pump
Standby Panel
Figure 6
ON/OFF Switch on Power Receptacle
14 INTRODUCTION |333/334 HPLC PUMPS
DESCRIPTION

Unpacking
The pump is delivered with most major components already assembled. Keep the original
container and packing assembly so the unit may be shipped safely, if necessary.
Carefully unpack the pump and its accessories from the carton.
CAUTION
Because of their weight, you should take special care when handling the large cartons. Pump
modules are heavy and should be lifted from the carton with care, by two people. Instructions
describing the unpacking procedures can be found on and in the carton.
It is necessary for two people to lift either the 333 Pump or 334 Pump out of the box, using the
straps provided. The 333 Pump weighs approximately 33.1 kg (73 lbs.). The 334 Pump weighs
approximately 29 kg (64 lbs.). To avoid personal injury and for general safety, if moving or
lifting the system, always get another person to assist you. Always follow local health and
safety regulations.
Standard Equipment
After the instrument and the accessories have been unpacked, you should have the following:
●333 Pump and/or 334 Pump with H3 Pump Heads
●An accessory kit that includes:
○Solvent Bottle Tray
○GSIOC Cable (334 Pump only)
○Wrenches
○Glass Bottle
○Tubing and Fittings
○Bellows Mounting Tool
○Extra Fuses
○Power Cord
○Terminal Block Connectors (333 Pump only)
○Plumbing Kit for Piston Rinsing Chambers
DOCUMENTATION
Documents are provided on the supplied 333/334 HPLC Pumps Documentation USB.
Optional Accessories
●Column Holder
●Manual Injection Valve Holder
For part numbers and installation instructions for the optional accessories, refer to the dedicated
appendices.
15
333/334 HPLC PUMPS | USER’S GUIDE
UNPACKING

Technical Specifications
Please be aware of the following before operating the pump.
NOTICE
Changes or modifications to the pump not expressly approved by Gilson could void the
warranty.
This instrument complies with part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) This instrument may not cause harmful interference, and (2) this instrument must
accept any interference received, including interference that may cause undesired operation.
Shieldedcables must be used with the pump to ensure compliance with the FCC Class A limits.
333/334 HPLC Pumps
Specification Description
Pump Type Single Solvent Pump
Hydraulic System Reciprocating Piston Pump
Pump Head H3: Up to 200 mL/min
Flow Rate
Range - Single Pump
H3: 0.2–200 mL/min
Range (Recommended) - Two Pumps
H3: 2–200 mL/min
Increment
0.01 mL/min
Flow Accuracy ± 2%
Flow Precision ≤ 0.7% RSD
Gradient
Solvents
Two
Formation
High Pressure Mixing with Static Mixer as Part of Pressure,
Purge, and Mixing Module (PPMM)
Composition Increment
0.1%
Gradient Accuracy ± 2%
Gradient Precision ≤ 0.7% RSD
Operating Pressure H3: 5–210 bar (70–3040 psi)
Compressibility Compensation Settable Compensation range 0–2000 Mbar-1
Piston Seal Wash Pump Head Inlet and Outlet Ports to/from a Rinsing Chamber
TECHNICAL SPECIFICATIONS CONTINUED ON PAGE 17
16 INTRODUCTION |333/334 HPLC PUMPS
TECHNICAL SPECIFICATIONS

333/334 HPLC Pumps
Specification Description
Priming Manual with Built-in Purge Valve via Control Software or Syringe
Liquid Contact Materials
316L Stainless Steel, Sapphire, Ceramic, UHMWPE, PTFE, Ruby,
Titanium, FEP, PCTFE, ETFE
For more information, refer to
Liquid Contact Materials on page 93.
Control and Communication
Communication
GSIOC (Gilson Serial Input Output Channel)
Inputs (333 Pump)
Four Digital Inputs; Start/End; Pause/Resume; Input (Wait);
Emergency
Outputs (333 Pump)
Four Relay Outputs; 12V DC; One Output Channel for Pressure,
Flow, or Composition; One Analog Output Channel for Pressure
Sensor Reading
Software Control
TRILUTION® LC Software
Electrical
Line Voltage
90 –260 VAC
Frequency
50 or 60 Hz
Power Consumption
600 W
Environmental
Operating Temperature
333 Pump: 10°C to 40°C
334 Pump: 4°C to 40°C
Operating Humidity
15%–80%
Operating Altitude
Up to 2000 m (81 kPa or 604 mmHg)
Physical
Dimensions (W x D x H)
333 Pump: 26 x 41 x 50.7 cm (10.2 x 16.2 x 20 in.)
334 Pump: 26 x 41 x 38.7 cm (10.2 x 16.2 x 15.2 in.)
Weight
333 Pump: 33.1 kg (73 lbs.)
Shipping Weight: 36.3 kg (80 lbs.)
334 Pump: 29 kg (64 lbs.)
Shipping Weight 33.1 kg (73 lbs.)
Contact [email protected]om for the methods and conditions that were used to obtain
technical specifications.
17
333/334 HPLC PUMPS | USER’S GUIDE
TECHNICAL SPECIFICATIONS

Customer Service
Gilson, Inc. and its worldwide network of representatives provide customers with the following
types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your local Gilson representative. Specific contact
information can be found at www.gilson.com. To help us serve you quickly and efficiently,
please refer to Repair and Return Policies on page 58.
18 INTRODUCTION |333/334 HPLC PUMPS
CUSTOMER SERVICE

19
333/334 HPLC PUMPS | USER’S GUIDE
Chapter 2
CHAPTER2|INSTALLATION
This chapter describes the minimum connections required for operation (including procedures
for preparing piston rinse lines). The pumpsmust be set up and installed in the order described
in this chapter to avoid damaging the system.
TRILUTION® LC Software is the recommended controller for the pumps; however, manual
control can be achieved by controlling the 334 HPLC Pump with the control panel atop the
333 HPLC Pump.
Both pumps are delivered with the main hydraulic components installed. Solvent bottles can
be placed in the removable trays atop the pumps, on the bench, or on the floor. The pumps can
be stacked to conserve bench space.
IN THIS CHAPTER
●Electrical Connections | 20
●Cabling for TRILUTION LC Control | 24
●Cabling for Control from 333 Pump | 24
●Twin Pump Systems | 25
●Positioning the Pumps | 26
●Hydraulic Connections | 28

20 INSTALLATION |333/334 HPLC PUMPS
ELECTRICAL CONNECTIONS
Electrical Connections
Plug the power cords into the pump and all associated devices before making the communication
or hydraulic connections. Power is needed to prime the pump and piston rinse chambers prior
to use.
Rear Panel
All electrical connections are made on the rear panel. Also present on the rear panel are the
fan’s ventilation slots, which must never be obstructed in any way.
Figure 9
Rear Panel Communications Port and Legend for 333 HPLC Pump
Figure 10
334 HPLC Pump Rear Panel Diagram