Gilson microman e User manual

User’s Guide
EN
E

2TABLE OF CONTENTS | MICROMAN® E
TABLE OF CONTENTS
INTRODUCTION | 3
Parts Checklist | 4
Good Laboratory Practice (GLP) Features | 4
Description | 5
Operating Ranges and Material | 6
Specifications | 7
FITTING THE CAPILLARY PISTON | 9
PIPETTING | 11
Setting the Volume | 11
Aspirating | 12
Dispensing | 13
Ejecting the Capillary Piston | 13
TROUBLESHOOTING | 14
CLEANING AND DECONTAMINATION | 15
WARRANTY | 18
Before use, please carefully read
WARRANTY on page 17 of this
User’s Guide!
TABLE OF CONTENTS

INTRODUCTION
3
MICROMAN® E |USER’S GUIDE
TABLE OF CONTENTS | MICROMAN® E
Chapter 1
INTRODUCTION
The ergonomically designed MICROMAN® E
positive-displacement pipettes, along with
disposable capillary piston (CP) tips, provide
the highest precision when pipetting viscous,
volatile, or non-aqueous liquids. Capillary pistons
eliminate the air cushion between the sample and
disposable piston, ensuring the accuracy of the
pipette is not affected by the physical properties
of the sample.
Positive-displacement technology is the ideal
solution to prevent from aerosol contamination
or sample carry-over, thus protecting the pipette,
the user, and the sample.
MICROMAN E is available as variable volume
single-channel models, with six models covering
a volume range from 1 to 1000 µL. M25E, M50E,
and M250E, with thin and tall capillary pistons
(CPs) are especially suited for use with tall or
narrow vessels.
Designed with comfort in mind, MICROMAN E is
a must for every workflow and application thanks
to its main features:
●QuickSnap system for a direct fitting of the
CPs: fitting is as easy as fitting a tip on an air
displacement pipette
●Improved ergonomic handle shape that rests
comfortably in hand
●Volume control switch to avoid any
accidental volume change when pipetting
●Complete and reliable pipetting of
challenging fluids: performances remain
unaffected by the physical properties of the
fluids, or at different temperatures
●MICROMAN E and CPs are the strongest
barrier against residual sample
carryover, aerosol contamination, and
cross-contamination. They provide a triple
protection: you, your sample, and your
pipette are protected.

INTRODUCTION
4INTRODUCTION | MICROMAN® E
Parts Checklist
Verify that the following items are present:
●MICROMAN E,
●User’s Guide,
●Safety bag,
●Adhesive ID-tags (strip of 6),
●Capillary pistons (10),
●Certificate of conformity
(including barcode sticker).
Good Laboratory Practice (GLP)
Features
●No need to touch disposables
(capillary piston).
●Serial Number: engraved on
pipette body.
●Barcode: on the box and
with the certificate (can be
transferred).
●ID Tag (Application or User).
●Color-coded push button
indicating the volume range.
●Data matrix engraved on
pipette body.
●Gilson certificate of
conformity according
to ISO 8655.
Figure 1
Data Matrix and
Serial Number
Location

INTRODUCTION
5
MICROMAN® E |USER’S GUIDE
INTRODUCTION | MICROMAN® E
Description
Color-coded push button
indicating the volume
range for aspirating and
dispensing.
Body or handle
Capillary
Piston
Shaft
Thumbwheel for
setting the volume
Volumeter
Volume control switch
Data matrix code
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
9
Figure 2
MICROMAN® E Description

INTRODUCTION
6INTRODUCTION | MICROMAN® E
Operating Ranges and Material
Available Models
Materials
Model Part
Number
Capillary
Pistons Volume Range
M10E FD10001 CP10/CP10ST 1 µL to 10 µL
M25E FD10002 CP25/CP25ST 3 µL to 25 µL
M50E FD10003 CP50/CP50ST 20 µL to 50 µL
M100E FD10004 CP100/CP100ST 10 µL to 100 µL
M250E FD10005 CP250/CP250ST 50 µL to 250 µL
M1000E FD10006 CP1000/CP1000ST 100 µL to 1000 µL
E
F
C
D
A
A
B
MICROMAN® E
Model Spring
(A)
Clamp
(B)
Shaft
(C)
Body
(D)
M10E SS Be Alloy PBT PVDF/PP
M25E SS Be Alloy PVDF PVDF/PP
M50E SS Be Alloy PVDF PVDF/PP
M100E SS Be Alloy PBT PVDF/PP
M250E SS Be Alloy PVDF PVDF/PP
M1000E SS Be Alloy PVDF PVDF/PP
CAPILLARY PISTONS
Model Capillary
(E)
Piston
(F)
CP10 PP PES
CP25 PP PES
CP50 PP PES
CP100 PP PE
CP250 PP PE
CP1000 PP POM
Abbreviations
SS = Stainless Steel
Be = Beryllium
PBT = Polybutylene Terephthalate
PP = Polypropylene
PVDF = Polyvinylidene Fluoride
PES = Polyester
PE = Polyethylene
POM = Polyacetal
NOTE
For more information on chemical
resistance of plastic materials, please refer
to the LT800550 Gilson Guide to Pipetting -
Appendix E, on www.gilson.com.

INTRODUCTION
7
MICROMAN® E |USER’S GUIDE
INTRODUCTION | MICROMAN® E
Specifications
MICROMAN E is a high quality pipette that offers
excellent accuracy and precision. The figures
provided in the Gilson Maximum Permissible Errors
on page 8, table were obtained using Gilson CPs.
These figures are only guaranteed when using
genuine Gilson CPs.
Each pipette is inspected and validated by qualified
technicians according to the Gilson quality system.
Gilson declares that its manufactured pipettes
comply with the requirements of the ISO 8655
standard, by type testing. The adjustment is carried
out under strictly defined and monitored conditions
(ISO 8655-6):
●Basis of adjustment, Ex.
●Reference temperature, 20°C
●Relative humidity, 50%
●Barometric pressure, 101 kPa
●Use of distilled water grade 3 (ISO 3696)
●Ten measurements for each test volume,
which are nominal volume, 50% of nominal
volume, and the minimum or 10% of nominal
volume.
Performance Tests
Each pipette is inspected and validated according
to the Gilson quality assurance system. Based
on extensive historical data, manufacturing
conditions, and expertise, and in compliance with
ISO standards relative to statistical process control,
the assurance level of this instrument performing
to specifications is 99.8%. Specifications rely on
quality and consistency for the whole pipetting
system and are guaranteed only when the pipette is
used with Gilson capillary pistons.

INTRODUCTION
8INTRODUCTION | MICROMAN® E
Gilson Maximum Permissible Errors
MICROMAN® E
Nom.
Vol.
(µL)
Vol.
(µL)
Vol.
(%)
Maximum Permissible Errors
Gilson ISO 8655-2 (Table 3)
Systematic
Error
(μL)
Random
Error
(μL)
Systematic
Error
(%)
Random
Error
(CV)*
Systematic
Error
(μL)
Random
Error
(μL)
Systematic
Error
(%)
Random
Error
(CV)*
M10E (P/N FD10001) WITH CP10 AND CP10ST
10
1
5
10
10
50
100
± 0.090
± 0.100
± 0.150
≤0.030
≤0.030
≤0.060
± 9.0
± 2.0
± 1.5
≤3.00
≤0.60
≤0.60
± 0.200
± 0.200
± 0.200
≤0.100
≤0.100
≤0.100
± 20
± 4.0
± 2.0
≤10
≤2.0
≤1.0
M25E (P/N FD10002) WITH CP25 AND CP25ST
25
3
10
25
12.0
40.0
100
± 0.25
± 0.27
± 0.30
≤0.080
≤0.080
≤0.100
± 8.3
± 2.7
± 1.2
≤2.67
≤0.80
≤0.40
± 0.35
± 0.35
± 0.35
≤0.15
≤0.15
≤0.15
± 11.67
± 3.5
± 1.4
≤5.0
≤1.5
≤0.60
M50E (P/N FD10003) WITH CP50 AND CP50ST
50 20
50
40.0
100
± 0.34
± 0.70
≤0.20
≤0.30
± 1.7
± 1.4
≤1.00
≤0.60
± 0.70
± 0.70
≤0.30
≤0.30
± 3.5
± 1.4
≤1.5
≤0.60
M100E (P/N FD10004) WITH CP100 AND CP100ST
100
10
50
100
10
50
100
± 0.50
± 0.75
± 1.00
≤0.20
≤0.30
≤0.40
± 5.0
± 1.5
± 1.0
≤2.00
≤0.60
≤0.40
± 1.4
± 1.4
± 1.4
≤0.6
≤0.6
≤0.6
± 14
± 2.8
± 1.4
≤6.0
≤1.2
≤0.60
M250E (P/N FD10005) WITH CP250 AND CP250ST
250
50
100
250
20.0
40.0
100
± 1.50
± 1.70
± 2.50
≤0.30
≤0.30
≤0.50
± 3.0
± 1.7
± 1.0
≤0.60
≤0.30
≤0.20
± 3.0
± 3.0
± 3.0
≤1.0
≤1.0
≤1.0
± 6.0
± 3.0
± 1.2
≤2.0
≤1.0
≤0.40
M1000E (P/N FD10006) WITH CP1000 AND CP1000ST
1000
100
500
1000
10
50
100
± 3.0
± 5.0
± 8.0
≤1.6
≤2.5
≤4.0
± 3.0
± 1.0
± 0.8
≤1.60
≤0.50
≤0.40
± 12
± 12
± 12
≤4.0
≤4.0
≤4.0
± 12
± 2.4
± 1.2
≤4.0
≤0.80
≤0.40
*CV means Coecient of Variation (%)
NOTE
With a precise pipetting technique, the M25E
model can be used to aspirate volumes as low
as 2.5 µL (10% of the nominal volume of the
pipette).

FITTING THE CAPILLARY PISTON
9
MICROMAN® E |USER’S GUIDE
INTRODUCTION | MICROMAN® E
CP10, CP25, CP50, CP100, CP250, and
CP1000 TIPACK
Capillary pistons (CP) are delivered ready to use.
To fit a capillary piston:
1. Press the MICROMAN E onto the capillary
piston until it is firmly seated 1.
2. Pick up the CP from the rack 2.
3. Slowly press the push button until you feel
and hear a slight click and continue to press
to the first stop 3.
4. Pre-wet the CP, and then aspirate the liquid.
5. Press the push button on the second stop to
eject the CP 3.
Chapter 2
FITTING THE CAPILLARY
PISTON
Make sure the capillary piston (CP) is compatible
with the MICROMAN E model that will be used.
MICROMAN® E
Nom.
Vol.
(µL)
Vol.
(µL)
Vol.
(%)
Maximum Permissible Errors
Gilson ISO 8655-2 (Table 3)
Systematic
Error
(μL)
Random
Error
(μL)
Systematic
Error
(%)
Random
Error
(CV)*
Systematic
Error
(μL)
Random
Error
(μL)
Systematic
Error
(%)
Random
Error
(CV)*
M10E (P/N FD10001) WITH CP10 AND CP10ST
10
1
5
10
10
50
100
± 0.090
± 0.100
± 0.150
≤0.030
≤0.030
≤0.060
± 9.0
± 2.0
± 1.5
≤3.00
≤0.60
≤0.60
± 0.200
± 0.200
± 0.200
≤0.100
≤0.100
≤0.100
± 20
± 4.0
± 2.0
≤10
≤2.0
≤1.0
M25E (P/N FD10002) WITH CP25 AND CP25ST
25
3
10
25
12.0
40.0
100
± 0.25
± 0.27
± 0.30
≤0.080
≤0.080
≤0.100
± 8.3
± 2.7
± 1.2
≤2.67
≤0.80
≤0.40
± 0.35
± 0.35
± 0.35
≤0.15
≤0.15
≤0.15
± 11.67
± 3.5
± 1.4
≤5.0
≤1.5
≤0.60
M50E (P/N FD10003) WITH CP50 AND CP50ST
50 20
50
40.0
100
± 0.34
± 0.70
≤0.20
≤0.30
± 1.7
± 1.4
≤1.00
≤0.60
± 0.70
± 0.70
≤0.30
≤0.30
± 3.5
± 1.4
≤1.5
≤0.60
M100E (P/N FD10004) WITH CP100 AND CP100ST
100
10
50
100
10
50
100
± 0.50
± 0.75
± 1.00
≤0.20
≤0.30
≤0.40
± 5.0
± 1.5
± 1.0
≤2.00
≤0.60
≤0.40
± 1.4
± 1.4
± 1.4
≤0.6
≤0.6
≤0.6
± 14
± 2.8
± 1.4
≤6.0
≤1.2
≤0.60
M250E (P/N FD10005) WITH CP250 AND CP250ST
250
50
100
250
20.0
40.0
100
± 1.50
± 1.70
± 2.50
≤0.30
≤0.30
≤0.50
± 3.0
± 1.7
± 1.0
≤0.60
≤0.30
≤0.20
± 3.0
± 3.0
± 3.0
≤1.0
≤1.0
≤1.0
± 6.0
± 3.0
± 1.2
≤2.0
≤1.0
≤0.40
M1000E (P/N FD10006) WITH CP1000 AND CP1000ST
1000
100
500
1000
10
50
100
± 3.0
± 5.0
± 8.0
≤1.6
≤2.5
≤4.0
± 3.0
± 1.0
± 0.8
≤1.60
≤0.50
≤0.40
± 12
± 12
± 12
≤4.0
≤4.0
≤4.0
± 12
± 2.4
± 1.2
≤4.0
≤0.80
≤0.40
Figure 3
CP fitting on MICROMAN® E
Click: The CP is attached
Press to the 1st stop: the
pipette is ready to aspirate
Press to the 2nd stop: the
CP is ejected
3
1
2
NOTE
Pre-wetting the CP before pipetting helps
prepare the CP for the best pipetting
performance. Pre-wetting the CP helps ensure
that volumes that you pipette will achieve
accuracy and precision within specifications.

FITTING THE CAPILLARY PISTON
10 FITTING THE CAPILLARY PISTON | MICROMAN® E
CP25, CP50, and CP250 Bulk
The capillaries and pistons are delivered in two
separate boxes.
To fit a capillary piston:
1. Take care not to
damage the sealing
tip 1when handling
the piston 3.
2. Select a piston 3
and slide it into the
capillary 4.
3. Gently push the
capillary until it snaps
onto the capillary
holder.
4. Attach the stem 2
by slowly pressing
MICROMAN E’s push
button until you feel
and hear a slight click
and then continue to
press to the first stop.
5. Pre-wet the CP, and
then aspirate the
liquid.
1
4
3
2
Figure 4
MICROMAN® E
Capillary Piston
Never lubricate the capillary holder,
capillary, or any other part of the
pipette. If the capillary tends to slip
o the capillary holder, clean the
capillary holder with ethanol, using
a medical wipe or similar soft tissue.

PIPETTING
11
MICROMAN® E |USER’S GUIDE
FITTING THE CAPILLARY PISTON | MICROMAN® E
Top
1
2
Chapter 3
PIPETTING
Setting the Volume
The volume of liquid to be aspirated is set using
the volumeter. The volumeter consists of three
number dials, which are read from top (most
significant digit) to bottom (least significant digit).
A marker is used to set exact or intermediate
volumes using the scale on the bottom dial.
The dials are colored either black or red to indicate
the position of the decimal point, according to the
model (see examples).
The volume is set by turning the thumbwheel
slowly to reach the required setting.
COLOR OF VOLUMETER NUMBERS
MODEL BLACK RED INCREMENT
M10E, M25E, M50E µL 0.1 µL 0.02 µL
M100E, M250E µL none 0.2 µL
M1000E 0.1 and 0.01 mL mL 2 µL
For small volume changes, directly and
slowly turn the thumbwheel. Fine volume
adjustments can be done without sliding
the volume control switch. To obtain
maximum accuracy when setting the
volume, proceed as follows:
●when decreasing the volume
setting, slowly reach the required
setting, making sure not to
overshoot the mark.
●when increasing the volume setting,
pass the required value by 1/3 of
a turn and then slowly decrease to reach the
volume, making sure not to pass the setting.
8
3
6
8
36.8 µL
M50
0
6
6.8 µL
M25
1
6
8
168 µL
M250
0
7
5
0.75 mL
M1000
E E
E E
0
6
8
6.8 µL
M10
0
6
8
68 µL
M100
E
E
0
6
8
6.8 µL
M10
0
6
8
68 µL
M100 E E
8
3
6
8
36.8 µL
M50
0
6
6.8 µL
M25
1
6
8
168 µL
M250
0
7
5
0.75 mL
M1000
E E E E
NOTE
The volume control switch is a safety to
prevent accidental volume change. The
thumbwheel can still be turned when the
switch is not slid, but the pipetting rod is much
harder to turn, and you will hear click sounds.

PIPETTING
12 PIPETTING | MICROMAN® E
For large volume changes, use the volume control
switch and proceed as follows:
1. Slide and hold the
volume control
switch for an easy
and quick volume
setting. The
thumbwheel will
turn faster, without
resistance and click.
2. When decreasing the
volume setting, approach
the required setting,
by 1/3 of a turn. When
increasing the volume
setting, pass the required
setting by 1/3 of a turn.
3. Release the unlock button button.
Pre-Wetting the CP
Greater uniformity and precision of dispensing
are usually obtained by providing identical
contact surfaces for all aliquots. This is achieved
by pre-wetting with the same liquid that is being
dispensed.
To pre-wet, immerse the CP in the liquid and
perform one pipetting step. Liquid can be
dispensed back into the original reservoir or to
waste.
Aspirating
1. Press the push button
to the first stop (refer to
Figure 5 on page 13),
2. Immerse the capillary
2 mm into the liquid,
3. Slowly release the push
button to draw up the
liquid (rest position),
4. Wipe any liquid from the
outside of the capillary,
taking care not to touch
the orifice.

PIPETTING
13
MICROMAN® E |USER’S GUIDE
PIPETTING | MICROMAN® E
Dispensing
Place the end of the capillary against the inside
wall of the recipient vessel,
1. Press the push button slowly to the first stop
(refer to Figure 5 below),
2. Keeping the push button depressed, move
the capillary away from the sidewall,
Withdraw MICROMAN E from the vessel and
release the push button.
NOTE
Unlike air-displacement pipettes,
MICROMAN E does not have any air
cushion and there is no purge needed.
Pushing the push button to the second
stop will eject the CP.
Ejecting the Capillary Piston
●Once the push button has been pressed
to the first stop, press down harder to the
second stop; at this point the piston and
capillary are ejected simultaneously (refer to
Figure 5 below).
NOTE
MICROMAN E is automatically
adjusted when the capillary piston is
correctly fitted. Once the volume is set,
measurements will be accurate and
precise.
Figure 5
Positive-Displacement - Forward Mode Pipetting
Rest
position
First Stop
Second
Stop
(ejection)
Prepare Aspirate Dispense Eject

TROUBLESHOOTING
14 TROUBLESHOOTING | MICROMAN® E
Chapter 4
TROUBLESHOOTING
You may be able to identify and correct the
problem by referring to the following information.
If you cannot solve the problem, please contact
your local Gilson representative.
Leaks:
Replace the capillary piston.
No stroke:
The piston is not properly fitted into the clamp;
check that the piston is seated correctly (refer to
page 9). Without any capillary piston, press the
push button to the first stop, and then press down
harder to the second stop.
Dicult to fit a capillary:
Clean the capillary holder with ethanol. Without
any capillary piston, press the push button to
the first stop, and then press down harder to the
second stop.
Inaccuracy:
Make sure the capillary is correctly mounted on
the capillary holder (refer to CHAPTER 2, FITTING
THE CAPILLARY PISTON, pages 9).
Imprecision:
Replace the capillary piston.
Dicult to set the volume:
This suggests internal damage to the pipette.
Contact your local Gilson representative.
If the problem persists...
Contact your local Gilson representative.

CLEANING AND DECONTAMINATION
15
MICROMAN® E |USER’S GUIDE
TROUBLESHOOTING | MICROMAN® E
Chapter 5
CLEANING AND
DECONTAMINATION
MICROMAN E is designed so that parts normally
in contact with liquid contaminants are easy to
clean and decontaminate.
Cleaning
Clean the pipette with soap solution prior to
decontamination.
External
1. Wipe the entire pipette with a soft cloth or
lint-free tissue soaked with soap solution
to remove any stains. If the pipette is very
dirty, a brush with soft plastic bristles may
be used.
2. Wipe the entire pipette with a soft cloth or
lint-free tissue soaked with distilled water.
3. Leave the parts to dry by evaporation or
wipe them down with a clean soft cloth or
lint-free tissue.
Internal
Only the following components should be
immersed in a decontaminant solution: clamp
assembly, return spring, and capillary holder
(shaft).
1. Unscrew the capillary holder (shaft).
2. Remove the clamp assembly and return
spring from the shaft.
3. Set aside the body (handle) in a dry and
secure location.
Before returning any pipette,
ensure that it is completely
free of chemical, biological, or
radioactive contamination. Use
the safety bag provided by Gilson.
WARNING

TROUBLESHOOTING
16 TROUBLESHOOTING | MICROMAN® E
4. Clean the individual components using an
ultrasonic bath (for 20 minutes at 50°C)
or with a soft cloth and brush. Small round
brushes with soft plastic bristles may be
used to clean the interior of the shaft.
5. Rinse the individual components with
distilled water.
6. Leave the parts to dry by evaporation or
wipe them down with a clean soft cloth or
lint-free tissue.
7. Reassemble the pipette.
Decontamination
You may refer to the Decontamination Procedure
LT802288 available at www.gilson.com.
Chemical Decontamination
Clean the pipette prior to decontamination.
Full details of recommended decontamination
procedures for Gilson pipettes are available from
your supplier. Whichever decontaminant you
use, check with the supplier that the product
is compatible with the materials used for the
component parts of the pipette, and that does not
attack any of the following plastics and metals:
polyester, PVDF (polyvinylidene fluoride), PP
(polypropylene), PBT (polybutylene terephthalate),
PC (polycarbonate), SS (stainless steel), and BE
Alloy (beryllium)
Non-immersible Parts
1. Wipe the body (handle) of the pipette with a
soft cloth or lint-free tissue impregnated with
the chosen decontaminant.
2. Wipe the body (handle) of the pipette with a
soft cloth or lint-free tissue impregnated with
distilled water or sterilized water.
Immersible Parts
Only the following components should be
immersed in a decontaminant solution: clamp
assembly, return spring and capillary holder (shaft).
1. Unscrew the capillary holder (shaft).
2. Remove the clamp assembly and return
spring from the shaft.
3. Set aside the body (handle) in a dry and
secure location.

TROUBLESHOOTING
17
MICROMAN® E |USER’S GUIDE
TROUBLESHOOTING | MICROMAN® E
4. Immerse the components in the
decontaminant solution or wipe them down
according to the instructions provided
by the manufacturer or supplier of the
decontaminant.
5. Rinse the individual components with
distilled or sterilized water.
6. Leave the parts to dry by evaporation or
wipe them with a clean lint-free tissue or a
soft cloth.
7. Reassemble the pipette.

WARRANTY
18 WARRANTY | MICROMAN® E
Chapter 6
WARRANTY
Gilson warrants this pipette against defects
in material under normal use and service for a
period of 12 months from the date of purchase.
This warranty shall not apply to pipettes which
are subject to abnormal use and/or improper
or inadequate maintenance (contrary to the
recommendations given in the User’s Guide),
including, but not limited to pipettes which have
been subjected to physical damage, improper
handling, spillage or exposure to any corrosive
environment. This warranty shall also be void
in the event pipettes are altered or modified by
any party other than Gilson or its designates.
Gilson’s sole liability under this warranty shall
be limited to, at Gilson’s sole option, repair or
replacement of any defective components of
pipettes or refund of the purchase price paid for
such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE
AND GILSON HEREBY DISCLAIMS ALL OTHER
WARRANTIES, WHETHER EXPRESS OR
IMPLIED, INCLUDING ANY WARRANTIES OF
MERCHANTABILITY AND ANY WARRANTIES
OF FITNESS FOR A PARTICULAR PURPOSE,
UNDER NO CIRCUMSTANCES SHALL GILSON
BE LIABLE FOR ANY CONSEQUENTIAL,
PUNITIVE, INDIRECT OR INCIDENTAL
DAMAGES ARISING OUT OF ANY BREACH
OF ANY EXPRESS OR IMPLIED WARRANTY .

NOTES
19
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