Hermle Z 327 K User manual

HERMLE Z 327 K
User Manual

CONTENTS
© Hermle Labortechnik GmbH Z327K_V1.22_eng I
1. PRODUCT DESCRIPTION ..................................................................................................1
1.1 Safety Instructions ............................................................................................................................................ 1
1.2 Intended Purpose .............................................................................................................................................. 1
1.3 Brief Description................................................................................................................................................ 1
1.4 Delivery Package ............................................................................................................................................... 1
1.5 Installation of the Centrifuge ............................................................................................................................ 2
1.5.1 Unpacking the Centrifuge................................................................................................................................ 2
1.5.2 Space Requirements....................................................................................................................................... 2
1.5.3 Installation ....................................................................................................................................................... 2
1.6 Signs and Indications of the Centrifuge .......................................................................................................... 3
1.6.1 General............................................................................................................................................................ 3
1.6.2 Product Nameplate (Example) ........................................................................................................................ 3
1.6.3 Warning and Information Signs ....................................................................................................................... 4
1.6.4 Danger, Precautions and Warranty ................................................................................................................. 4
1.6.5 Following Rules Must Strictly be Adhered To:................................................................................................. 5
1.6.6 Warranty.......................................................................................................................................................... 5
1.7 Operating and Display Elements...................................................................................................................... 6
1.7.1 LED light......................................................................................................................................................... 6
1.7.2 LCD-Display .................................................................................................................................................... 7
1.8 Basic Adjustments ............................................................................................................................................ 8
1.8.1 Access to the Mode: "Standard settings" ........................................................................................................ 8
1.8.2 Temperature Indication.................................................................................................................................... 9
1.8.3 Sound Signal Turn On / Off ............................................................................................................................. 9
1.8.4 Volume Pre-Selection of Sound Signal ......................................................................................................... 10
1.8.5 Song Selection - End of Run......................................................................................................................... 10
1.8.6 Keyboard Sound Turn On / Off...................................................................................................................... 11
1.8.7 Setting of the standby turn-off time................................................................................................................ 11
1.8.8 Retrieving Operating Data............................................................................................................................. 12
2. OPERATION......................................................................................................................14
2.1 Mounting and Loading the Angle Rotor ........................................................................................................ 14
2.1.1 Installation of Rotors...................................................................................................................................... 14
2.1.2 Loading Angle Rotors.................................................................................................................................... 14
2.1.3 Loading Swing Out Rotors............................................................................................................................. 15
2.1.4 Loading and Overloading of Rotors............................................................................................................... 16
2.1.5 Removing the Rotor ...................................................................................................................................... 16
2.2 Lid ..................................................................................................................................................................... 17
2.2.1 Lid Release ................................................................................................................................................... 17
2.2.2 Lid Lock......................................................................................................................................................... 17
2.3 Pre-Selection.................................................................................................................................................... 18
2.3.1 Pre-Selection of Speed / RCF-Value............................................................................................................. 18
2.3.2 Pre-Selection of Running Time...................................................................................................................... 18
2.3.3 Pre-Selection of Brake Intensity and Acceleration........................................................................................ 19
2.3.4 Pre-Selection of Temperature ....................................................................................................................... 20

CONTENTS
II Z327K_V1.22_eng © Hermle Labortechnik GmbH
2.3.5 Pre-Cooling ................................................................................................................................................... 20
2.4 Radius Correction............................................................................................................................................ 21
2.5 Program............................................................................................................................................................ 21
2.5.1 Storage of Programs ..................................................................................................................................... 21
2.5.2 Recall of Stored Programs ............................................................................................................................ 22
2.5.3 Leaving Program Mode................................................................................................................................. 22
2.6 Starting and Stopping the Centrifuge............................................................................................................ 23
2.6.1 Starting the Centrifuge................................................................................................................................... 23
2.6.2 The "STOP" Key............................................................................................................................................ 23
2.7 Imbalance Detection........................................................................................................................................ 24
2.8 Timer Function................................................................................................................................................. 24
3. Maintenance......................................................................................................................26
3.1 Maintenance and Cleaning.............................................................................................................................. 26
3.1.1 General Care:................................................................................................................................................ 26
3.1.2 Cleaning and Disinfecting of the Unit ............................................................................................................ 27
3.1.3 Cleaning and Disinfecting of the Rotor.......................................................................................................... 27
3.1.4 Disinfection of Aluminum Rotors ................................................................................................................... 27
3.1.5 Disinfection of PP-Rotors .............................................................................................................................. 27
3.1.6 Glass Breakage............................................................................................................................................. 28
3.2 Lifetime of Rotors, Round and Rectangular Buckets, Accessories............................................................ 28
4. Trouble Shooting..............................................................................................................29
4.1 Error Messages: Problem / Solution .............................................................................................................. 29
4.2 Survey of Possible Error Messages and Solutions ...................................................................................... 29
4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 29
4.2.2 Description of the Error Message System..................................................................................................... 30
4.2.3 Procedure while error 14 ............................................................................................................................... 30
4.2.4 Procedure while error 38 –Lid motor is blocked ........................................................................................... 31
5. Receipt of Centrifuges for Repair....................................................................................32
6. TRANSPORT, STORAGE AND DISPOSAL......................................................................33
6.1 Transport.......................................................................................................................................................... 33
6.2 Storage ............................................................................................................................................................. 33
6.3 Disposal............................................................................................................................................................ 33
7. APPENDIX........................................................................................................................... I
EC - Conformity Declaration .................................................................................................................................... II
Table 1: Technical Data .......................................................................................................................................... III
Table 2: Abbreviations ............................................................................................................................................IV
Table 3: Permissible Net Weight.............................................................................................................................IV
Table 4: Lowest Temperature at Max. Speed ..........................................................................................................V
Table 5: Max. Speed and RCF-Values for Permissible Rotors ...............................................................................VI
Table 6: Acceleration and Deceleration Times.......................................................................................................VII

CONTENTS
© Hermle Labortechnik GmbH Z327K_V1.22_eng III
Table 7: Error Messages.......................................................................................................................................VIII
Table 8: Radius Correction .....................................................................................................................................IX
Table 8(Part 2): Radius Correction...........................................................................................................................X
Table 8(Part 3): Radius Correction..........................................................................................................................XI
Redemption Form / Decontamination Certificate ..................................................................................................XIII

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 1
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage.
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories, listed in the technical documentation,
are in compliance with the Regulation (EU) 2017/746 of the European Parliament and of the Council
of 5 April 2017 on in-vitro-diagnostic medical devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type Z 327 K is a refrigerated universal centrifuge, which we offer in two voltage variations
230V or 120V.
The centrifuge can be used with swing-out rotors and angle rotors.
All parameters are accessible via buttons, and selected with a control field. All pre-selected and
current values will be shown, permanently, on the LCD-Display.
The centrifuge is powered by a Maintenance-Free Induction Motor.
Detailed technical data are in “Table 1: Technical Data" (see APPENDIX P.III).
1.4 Delivery Package
•1 Centrifuge Z 327 K
•1 Operating Manual Z 327 K
•1 Rotor Key
•1 Power Cord
Rotor(s) / Accessories will be packaged separately.

PRODUCT DESCRIPTION
2Z327K_V1.22_eng © Hermle Labortechnik GmbH
1.5 Installation of the Centrifuge
1.5.1 Unpacking the Centrifuge
Model Z 327 K is supplied in a carton. Remove the strap retainer, open the carton, and remove the
padding. Lift the centrifuge on both sides (see Figure 1) with an appropriate number of helpers and
place it on the laboratory table.
Attention! Do not lift the centrifuge from under the lid or by the front panel!
Figure 1
The instruction manual must be kept with the centrifuge, at all times!
1.5.2 Space Requirements
The centrifuge should be installed on an even, solid surface, if possible on a laboratory cabinet /
table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm/11.81in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.5.3 Installation
Follow These Steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's rating
label, mounted on the rear panel.
•The power connection for the centrifuge requires a separate one-site protection, with 16 A (Type K)
•In case of emergency, there must be an emergency switch off installed outside of the room, in order
to disconnect the power supply from the unit.
•Connect the centrifuge, with the mains.
(The socket for the power cord must be easy to reach, respectively easy to disconnect).
•Switch on, by using the mains power switch (I).
•Open the lid, by using the button LID.
• Remove the transport securing device of the motor.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 3
1.6 Signs and Indications of the Centrifuge
1.6.1 General
Direction of Rotation –clockwise rotation for the rotor drive
Reference for Loading Rotors
1.6.2 Product Nameplate (Example)
Company Address:
Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
In-Vitro-Diagnostic Medical Device
TYPE:
Type Designation of the Product
REF:
Order No. of the Product
SN:
Serial No. of the Product
Manufacturer
Date of Manufacture
MAX. Drehzahl:
Max. Speed Allowed of the Unit
KIN. EN.:
Max. Kinetic Energy with Corresponding Rotor
U/I/f:
Allowable Voltage / Max. Current / Frequency
P:
Electrical Input Power
Operating Manual Indication
Labeling, Standards and Guidelines
RoHS-Conformity
UK-Conformity Assessed
Instructions for Disposal, (see Chapter 6.3, P. 33

PRODUCT DESCRIPTION
4Z327K_V1.22_eng © Hermle Labortechnik GmbH
1.6.3 Warning and Information Signs
Four carrier must be used at all times on four places swing out rotors
or damage will occur to the centrifuge. Such damage will not be
covered under the product warranty
Attention! Check the fastening of the rotor nut before each run
Take off mains plug before opening the housing or the emergency
release
Power Input
Rotation Direction Emergency Release
Biohazard Warning
1.6.4 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken:
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of
EN 61010-2-020.
•The HERMLE Z 327 K, is "non-explosion proof" and must not be operated in explosion-
endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such
substances, which chemically react with high energy, is strictly prohibited. If used in such
environment, this is at the users own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures, in case
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 5
1.6.5 Following Rules Must Strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and buckets, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, that show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.
1.6.6 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to technical
improvement!

PRODUCT DESCRIPTION
6Z327K_V1.22_eng © Hermle Labortechnik GmbH
1.7 Operating and Display Elements
Figure 2
1control filed Run Parameters
20-I Power Switch
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration / Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
8quick Short Running
9start Start Centrifugation
10 stop Stop Centrifugation
11 prog Retrieving Programs
12 store Program Store
13 temp Temperature Indication
12 LED light Shows the status of the centrifuge
1.7.1 LED light
The LED light indicates the current operation state of the centrifuge. The following table shows all
operating states.
Color of LED light
Operating state
Green
Run complete, lid is closed
Yellow
Lid opened, no sleep mode
Red flashing fast
Error message
Red flashing slow
Centrifuge runs
Yellow flashing slow
Sleep mode
Red –Green flashing slow
Standard settings menu

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 7
1.7.2 LCD-Display
The following picture shows the individual elements of the LCD-display.
Figure 3
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field –"time"
Messages/Logos of the Display Fields:
M1 "close" M8 "decel"
M2 "open" M9 "radius"
M3 "rotor" M10 "program"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
M6 "rcf" M13 h m s
M7 "accel"
Indication:
After switching on the centrifuge, the display, "rpm/rcf"(A-1) shows the loading status, the current
software version and finally the model type (e.g. Z 36 HK).
Figure 4

PRODUCT DESCRIPTION
8Z327K_V1.22_eng © Hermle Labortechnik GmbH
1.8 Basic Adjustments
1.8.1 Access to the Mode: "Standard settings"
At commissioning of the centrifuge, you have the options to make the following basic changes:
-Temperature Indication in °C or °F
-Acoustic Signal Turn on / off
-Volume Pre-Selection of Sound Signal
-Song Selection of Sound Signal "end of run"
-Keyboard Sound Turn on / off
-Setting of the standby turn-off time
The following operating data can be retrieved in this menu:
-Number of starts
-Operating hours of centrifuge
-Operating hours of motor
-Software version centrifuge
-Software version frequency converter
-Error list
-Function of the imbalance sensor
-Operation of keyboard
-Hardware version
-Serial number of the control board
-Number of rotor starts of the mounted rotor
-Number of operating hours of the mounted rotor
-Update touch control panel (only for service engineers)
Open the centrifuge lid and shut off the main switch. Now switch on again the main switch. For
approximately 3 seconds the software version and the model type will be shown in the display (see
Figure 4). Press during this time the keys “time“ (6) and “lid“ (7) simultaneously. As a result, a
display test is administered for approx. 3 seconds. All possible indications will appear at the same
time (see Figure 5). The LED light is flashing alternately with green and red color.
Figure 5
Attention:
-The normal program mode can be changed back again by switching off the centrifuge, for a
short period!
-All changed settings must be confirmed by the key “store” (12) or “start” (9). A confirmation
screen will appear with the word, “store”, in the display “rpm/rcf” (A-1), - Only then the pre-
selections are valid (see Figure 6).

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 9
Figure 6
1.8.2 Temperature Indication
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key "accel/decel" (5).
In the display, "accel/decel" (A-2) appears the word, "service". Select the letter, "C", with the control
field (1). As a result, appearing in the display, "rpm/rcf" (A-1) are the words, "°C/temp". By pressing
the key, "rpm/rcf" (4), the word "°C" flashes, this allows you to change the display into Fahrenheit
"°F", with the control field (1), (see Figure 7).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 7
1.8.3 Sound Signal Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key; "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the
control field (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound". By
pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the
control field (1), (see Figure 8).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
1
7
2
3

PRODUCT DESCRIPTION
10 Z327K_V1.22_eng © Hermle Labortechnik GmbH
Figure 8
1.8.4 Volume Pre-Selection of Sound Signal
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key "accel/decel" (5). In the
display, "accel/decel" (A-2), flashes the word, "service". Select the letter, "U" with the control field (1). As a result,
appearing in the display, "rpm/rcf" (A-1), are the words, "Vol=0- 9/Sound". By pressing the key, "rpm/rcf" (4), the
desired volume can be adjusted between 0 (low) and 9 (loud), with the control field (1), (see Figure 9
).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 9
1.8.5 Song Selection - End of Run
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "G" with the
control field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word " SonGo/Sound". After
pressing the key „rpm/rcf“ (4), select a song with the control field (1), (see Figure 10).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
9
8

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 11
Figure 10
1.8.6 Keyboard Sound Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display; "accel/decel" (A-2) flashes the word, "service". Select the letter, "b" with the
control field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". By
pressing the key, "rpm/rcf" (4), the keyboard sound (On) or (Off) can be turned on, with the control
field (1), (see Figure 11).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 11
1.8.7 Setting of the standby turn-off time
After not using the centrifuge for 5 minutes, the centrifuge automatically falls into the standby mode.
The display is switching off and the LED light is flashing slowly with yellow color. By pressing any
key, the centrifuge will be reactivated. The sleep mode can be deactivated or set in the range
between 1 –60 minutes.
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter "I" with the control
field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "Stby" (see Figure 12 ) . After
pressing the key „rpm/rcf“, the standby mode can be turned off or pre-set to the needed value by
turning the control field (1).

PRODUCT DESCRIPTION
12 Z327K_V1.22_eng © Hermle Labortechnik GmbH
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 12
1.8.8 Retrieving Operating Data
In the function, "Basic Adjustments", retrieving the operating data of the centrifuge is an available
option. Please proceed as illustrated, under point 1.8.1, to enter this program mode, press the key,
"accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the control filed (1), the following information can be retrieved:
A = Number of starts of the centrifuge
H = Operating hours of the centrifuge
h = Operating hours of the motor
S = Software version centrifuge
r = Software versions frequency converter
E = List of previous error message
F = Function of the imbalance sensor
P = Operation of keyboard
d = Hardware version
y = Serial number and manufacturing year of the control board
N = Number of rotor starts of the mounted rotor
o = Number of operating hours of the mounted rotor
n = Update touch control panel (only for service engineers)
The list of the last 99 error messages can be reviewed by pressing the key, "rpm/rcf" (4) and scroll
through, with the control field (1) (see Figure 13). The respective error codes appear in the display,
"rpm/rcf" (A-1). Please refer to “Table 7: Error Messages“, (see APPENDIX P. VIII).
Switch off the centrifuge, to return to the normal program mode.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z327K_V1.22_eng 13
Figure 13
While retrieving the number of rotor starts and operating hours of the mounted rotor, as a result,
appearing in the display “rpm/rcf” (A-1), the word “r-StA” respectively “r-hou” (see Figure 14).
Figure 14

OPERATION
14 Z327K_V1.22_eng © Hermle Labortechnik GmbH
2. OPERATION
2.1 Mounting and Loading the Angle Rotor
2.1.1 Installation of Rotors
Clean the drive shaft and the connection surface of the rotor with a clean, grease-free piece of cloth.
Place the rotor onto the drive shaft, (see Figure 15). Please be sure that the rotor is fully installed
onto the motor shaft.
Figure 15 Figure 16
Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing nut clockwise.
Tighten the fixing nut with the provided Allen key, (see Figure 16 and Figure 17).
Figure 17
ATTENTION: For safety, always ensure that the rotor fixing screw is tightened before each
run (see Figure 16).
2.1.2 Loading Angle Rotors
Rotors must be loaded symmetrically and with equal weight. The adapter may only be loaded with
the appropriate vessels. The weight differences between the filled vessels should be kept as low as
possible. Therefore, we recommend to weigh with a balance. This reduces the wear of drive and the
acoustic operating noise.
Each rotor indicates what the maximum capacity is per hole.