Hermle ZK 496 User manual

© Hermle Labortechnik GmbH ZK496_V2.19_eng
HERMLE ZK 496
User Manual

CONTENTS
© Hermle Labortechnik GmbH ZK496_V1.22_eng I
1. PRODUCT DESCRIPTION .................................................................................................. 4
1.1 Safety Instructions .............................................................................................................................................4
1.2 Intended Purpose ...............................................................................................................................................4
1.3 Brief Description.................................................................................................................................................4
1.4 Delivery Package ................................................................................................................................................4
1.5 Operating and Display Elements.......................................................................................................................5
1.5.1 LCD-Display .....................................................................................................................................................6
1.6 Signs and Indications of the Centrifuge ...........................................................................................................7
1.6.1 General.............................................................................................................................................................7
1.6.2 Product Nameplate (Example) .........................................................................................................................7
1.6.3 Warning and Information Signs ........................................................................................................................8
1.6.4 Danger, Precautions and Warranty ..................................................................................................................8
1.6.5 Following Rules Must strictly be Adhered To:.................................................................................................10
1.6.6 Warranty.........................................................................................................................................................10
1.7 Installation of the Centrifuge ...........................................................................................................................11
1.7.1 Unpacking the Centrifuge...............................................................................................................................11
1.7.2 Space Requirements......................................................................................................................................11
1.7.3 Installation ......................................................................................................................................................12
1.7.4 Attention before first use!................................................................................................................................13
1.8 Basic Adjustments ...........................................................................................................................................14
1.8.1 Access to Mode: "Operating Data".................................................................................................................14
1.8.2 Temperature Indication...................................................................................................................................15
1.8.3 Signal Turn On / Off .......................................................................................................................................16
1.8.4 Volume Pre-Selection of Sound Signal ..........................................................................................................16
1.8.5 Song Selection - End of Run..........................................................................................................................17
1.8.6 Keyboard Sound Turn On / Off.......................................................................................................................17
1.8.7 Retrieving Operating Data (operated by skilled or service engineer only!).....................................................18
2. OPERATION...................................................................................................................... 19
2.1 Mounting and Loading the Angle Rotor .........................................................................................................19
2.1.1 Installation of Rotors.......................................................................................................................................19
2.1.2 Loading Angle Rotors.....................................................................................................................................20
2.1.3 Loading Swing out Rotors ..............................................................................................................................20
2.1.4 Loading and Overloading of Rotors................................................................................................................21
2.1.5 Removing the Rotor .......................................................................................................................................21
2.2 Lid ......................................................................................................................................................................22
2.2.1 Lid Release ....................................................................................................................................................22
2.2.2 Lid Lock..........................................................................................................................................................22
2.3 Pre-Selection.....................................................................................................................................................23
2.3.1 Pre-Selection of Speed / RCF-Value..............................................................................................................23
2.3.2 Pre-Selection of Running Time.......................................................................................................................23
2.3.3 Pre-Selection of Brake Intensity and Acceleration.........................................................................................24
2.3.4 Pre-Selection of Temperature ........................................................................................................................25
2.3.5 Pre-Cooling ....................................................................................................................................................25
2.4 Radius Correction.............................................................................................................................................26

CONTENTS
II ZK496_V1.22_eng © Hermle Labortechnik GmbH
2.5 Program.............................................................................................................................................................26
2.5.1 Storage of Programs ......................................................................................................................................26
2.5.2 Recall of Stored Programs .............................................................................................................................27
2.5.3 Leaving Program Mode..................................................................................................................................28
2.6 Starting and Stopping the Centrifuge.............................................................................................................28
2.6.1 Starting the Centrifuge....................................................................................................................................28
2.6.2 The "STOP" Key.............................................................................................................................................28
2.7 Imbalance Detection.........................................................................................................................................29
2.8 Interface to the Computer ................................................................................................................................29
2.9 Timer-Function..................................................................................................................................................29
3. MAINTENANCE................................................................................................................. 31
3.1 Maintenance and Cleaning...............................................................................................................................31
3.1.1 General Care:.................................................................................................................................................31
3.1.2 Cleaning and Disinfecting of the Unit .............................................................................................................32
3.1.3 Cleaning and Disinfecting of the Rotor...........................................................................................................32
3.1.4 Disinfection of Aluminum Rotors ....................................................................................................................32
3.1.5 Disinfection of PP-Rotors ...............................................................................................................................33
3.1.6 Glass Breakage..............................................................................................................................................33
3.2 Lifetime of Rotors, Round and Rectangular Buckets, Accessories.............................................................33
4. TROUBLE SHOOTING ...................................................................................................... 33
4.1 Error Message: Problem / Solution.................................................................................................................33
4.2 Survey of Possible Error Messages and Solutions .......................................................................................34
4.2.1 Lid Release during Power Failure (Emergency Lid Release).........................................................................34
4.2.2 Description of the Error Message System......................................................................................................35
4.2.3 Procedure while error 14 from software version 1.76....................................................................................35
4.2.4 Procedure while error 38 –Lid motor is blocked ............................................................................................36
5. RECEIPT OF CENTRIFUGES FOR REPAIR..................................................................... 37
6. TRANSPORT. STORAGE AND DISPOSAL...................................................................... 38
6.1 Transport...........................................................................................................................................................38
6.2 Storage ..............................................................................................................................................................38
6.3 Disposal............................................................................................................................................................. 38
6.4 RoHS Declaration of Conformity.....................................................................................................................38
7. APPENDIX.......................................................................................................................... III
EG - Conformity Declaration ....................................................................................................................................IV
Table 1: Technical Data ............................................................................................................................................V
Table 2: Permissible Net Weight..............................................................................................................................VI
Table 3: Lowest Temperatures at Max. Speed ........................................................................................................VI
Table 4: Max Speed and RCF-Values for Permissible Rotors .................................................................................VI
Table 5: Acceleration and Deceleration Times........................................................................................................VII
Table 6: Error Messages........................................................................................................................................VIII
Table 7: Radius Correction ......................................................................................................................................IX

CONTENTS
© Hermle Labortechnik GmbH ZK496_V1.22_eng I
Table 8: Abbreviations ..............................................................................................................................................X
Redemption Form / Decontamination Certificate ...................................................................................................XIII

PRODUCT DESCRIPTION
4ZK496_V1.22_eng © Hermle Labortechnik GmbH
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage.
Intended use includes the observation of all instructions in the instruction manual and administering
inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge ZK 496 was designed for the separation of materials or mixtures with
different densities. It is to be used only for this purpose. The designated device and its' accessories,
listed in the technical documentation, are in compliance with the Regulation (EU) 2017/746 of the
European Parliament and of the Council of 5 April 2017 on in-vitro-diagnostic medical devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company Hermle Labortechnik is not liable.
1.3 Brief Description
The unit type ZK 496 is a refrigerated Floor-Standing Centrifuge, which is also available as an
Underbench version. This model comes only with one voltage option of 230 V.
The centrifuge can be used with swing-out rotors and angle rotors.
All parameters are accessible via buttons and selected with a central adjuster. All pre-selected and
current values will be shown permanently on the LCD-Display.
The centrifuge is powered by a Maintenance-Free Induction Motor.
Detailed technical data are in Table 1: Technical Data (see APPENDIX P.V).
1.4 Delivery Package
•1 Centrifuge ZK 496
•1 Operating Manual ZK 496
•1 Rotor key
•1 Power Cord
•1 Double open ended wrench
Rotor(s) / Accessories will be packaged separately

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 5
1.5 Operating and Display Elements
1central adjuster Run Parameters
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration / Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
8quick Short Running
9start Start Centrifugation
10 stop Stop Centrifugation
11 prog Retrieving Stored Programs
12 store Program Store
13 temp Temperature Indication

PRODUCT DESCRIPTION
6ZK496_V1.22_eng © Hermle Labortechnik GmbH
1.5.1 LCD-Display
The following picture shows the individual elements of the LCD-display.
Figure 1
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field –"time"
A-4 Display Field - "temp"
Messages/Logos of the Display Fields:
M1 "close" M8 "decel" M15 "precool"
M2 "open" M9 "radius"
M3 "rotor" M10 "program"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
M6 "rcf" M13 h m s
M7 "accel" M14 "temperature"

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 7
Indication:
After switching on the centrifuge, the display "rpm/rcf"(A-1) shows the loading status, the current
software version and finally the model type (e.g. Z 36 HK).
1.6 Signs and Indications of the Centrifuge
1.6.1 General
Instructions for Disposal (see chapter 6.3 P. 38)
Direction of Rotation –clockwise rotation for the rotor drive
Reference for Loading Rotors
1.6.2 Product Nameplate (Example)
Company Address:
Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
In-Vitro-Diagnostic Medical Device
TYPE:
Type Designation of the Product
REF:
Order No. of the Product
SN:
Serial No. of the Product
Manufacturer
Date of Manufacture
MAX. Drehzahl:
Max. Speed Allowed of the Unit

PRODUCT DESCRIPTION
8ZK496_V1.22_eng © Hermle Labortechnik GmbH
KIN. EN.:
Max. Kinetic Energy with Corresponding Roto
U/I/f:
Allowable Voltage / Max. Current / Frequency
P:
Electrical Input Power
Operating Manual Indication
UK-Conformity Assessed
Labeling, Standards and Guidelines
RoHS Compliance
1.6.3 Warning and Information Signs
Four carriers must be used at all times on four places swing
out rotors or damage will occur to the centrifuge. Such damage
will not be covered under the product warranty
Attention! Check the fastening of the rotor nut before each run
Take off mains plug before opening the housing or the
emergency release
Power Input
Rotation Direction Emergency Release
Biohazard Warning
1.6.4 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken.
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited within 30 cm/12 in around the centrifuge. According to the regulations of

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 9
EN 61010-2-020.•The HERMLE ZK 496 is "non-explosion proof" and must not be operated in
explosion-endangered areas or locations.

PRODUCT DESCRIPTION
10 ZK496_V1.22_eng © Hermle Labortechnik GmbH
Centrifugation of flammable, explosive, radioactive or such substances, which chemically
react with high energy, is strictly prohibited. If used in such environment, this is at the users
own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures in case
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating
with a speed of > 2m/s.
1.6.5 Following Rules Must strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge if mechanical or electrical assembly groups have been tampered
with by unauthorized personnel.
•Do not use accessories such as rotors and buckets that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes made of glass or plastic.
•Do not spin extremely corrosive substances as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets which show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability of the centrifuge only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.
1.6.6 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty for a period of two
years from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved by the manufacturer in regards to technical
improvement!

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 11
1.7 Installation of the Centrifuge
1.7.1 Unpacking the Centrifuge
Model ZK 496 is supplied in a carton on a wooden pallet. Remove the strap retainer and open the
carton. Remove the metal bars which are fixed onto the pallet to secure the centrifuge.
Remove the metal bars and fix them to the front edge of the pallet so that they can be used as a
ramp (see Figure 2). Use the same screws and pay attention to secure screw connection.
Take the provided open-ended spanner to move the centrifuge feet up until it is on its rollers (see
also figure 3).
Move the centrifuge carefully from the pallet, preferably with several people. Move the centrifuge on
its intended location. The instruction manual must be kept with the centrifuge at all times!
Figure 2
1.7.2 Space Requirements
The centrifuge has to be installed on an even, solid floor. During centrifugation, the centrifuge must
be placed in a way that there is a minimum space of 30 cm/11.81in on each side of the unit
according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat as the performance of the unit is based on an ambient temperature of 23°C/73.4°F. An
unobstructed exhaust of hot air from the cooling system of the centrifuge must be ensured.

PRODUCT DESCRIPTION
12 ZK496_V1.22_eng © Hermle Labortechnik GmbH
1.7.3 Installation
Follow These Steps:
•Move the centrifuge on its intended location. Unscrew the lock nut (1) with an open-ended spanner
AF M16 (see Figure 3). Turn the appliance feet (2) down with an open-ended spanner AF M13 until
they are firmly on the floor surface. Now the centrifuge has to be leveled horizontally, using the four
appliance feet. Therefore mount the associated rotor on the motor shaft and place a spirit level on it.
After leveling the appliance feet, tighten the lock nut. The rollers should not touch the ground
anymore.
Figure 3
•Check whether the power supply corresponds with the one specified on the manufacturer's rating
label.
•The power connection for the centrifuge requires a separate one-site protection 15 A. 16 A or 20 A
(Type K)
•In case of emergency, there must be an emergency switch off installed outside of the room in order
to disconnect the power supply from the unit.
•Connect the centrifuge with the mains.
(The socket for the power cord must be easy to reach respectively easy to disconnect).
•Switch on by using the mains power switch (I).
•Open the lid by using the button LID.
2
1

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 13
1.7.4 Attention before first use!
Depending on how the motor shaft of your centrifuge is looking like, some action must be taken
before the first operation. Please note the shape of the motor shaft of your centrifuge.
There are two different versions:
Version 1 Version 2
If the motor shaft is conical
remove the collet from the rotor by turning it
counter clockwise
The collet is no longer
needed
If the motor shaft is cylindrical
Let the collet inside the rotor
Conical
motor shaft
Cylindrical
motor shaft

PRODUCT DESCRIPTION
14 ZK496_V1.22_eng © Hermle Labortechnik GmbH
1.8 Basic Adjustments
At commissioning of the centrifuge, you have the options to make the following basic changes:
-Temperature Indication in °C or °F
-Acoustic Signal Turn On / Off
-Keyboard Sound Turn On / Off
-Volume Pre-Selection of Sound Signal
-Song Selection "end of run"
1.8.1 Access to Mode: "Operating Data"
If the centrifuge is still turned off, press the keys "time" (6) and "lid" (7) simultaneously turning on the
main switch of the centrifuge. Now release both keys. As a result, a display test is administered for
approx. 5 seconds. All possible indications will appear at the same time (see Figure 4).
Figure 4
ATTENTION:
-Please notice that you must enter the program as described under point 1.8.1.to change
the adjustments of the points 1.8.2 - 1.8.7. After the settings have been stored by user,
the normal program mode can be changed back again by switching off the centrifuge. for
a short period.
-All changed settings must be confirmed by the key "start" (9). A confirmation screen will
appear with the word "store" in the display box "rpm/rcf"(A-1) - Only then the pre-
selections are valid (see Figure 5)!

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 15
Figure 5
1.8.2 Temperature Indication
Proceed as illustrated under point 1.8.1. to enter this program mode, press the key "accel/decel" (5).
In the display "accel/decel" (A-2) appears the word "service". Select the letter "C". with the adjustable
knob (1). As a result appearing in the display "rpm/rcf" (A-1) are the words "CELSI/temp". By pressing
the key "rpm/rcf" (4) the word "CELSI" flashes. This allows you to change the display into Fahrenheit
"FAREN". with the adjustable knob (1) (see Figure 6).
After the settings have been stored by user, the normal program mode can be changed back again by
switching off the centrifuge for a short period.
Figure 6
2
3
1
7
2
3

PRODUCT DESCRIPTION
16 ZK496_V1.22_eng © Hermle Labortechnik GmbH
1.8.3 Signal Turn On / Off
Proceed as illustrated under point 1.8.1 to enter this program mode, press the key; "accel/decel" (5).
In the display "accel/decel" (A-2) flashes the word "service". Select the letter "L" with the adjustable
knob (1). As a result appearing in the display "rpm/rcf" (4) are the words "On Sound". By pressing
the key "rpm/rcf" (4) the word "On" flashes and the sound can be switched off with the adjustable
knob (1), (see Figure 7).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge for a short period.
Figure 7
1.8.4 Volume Pre-Selection of Sound Signal
Proceed as illustrated under point 1.8.1 to enter this program mode, press the key "accel/decel" (5).
In the display “accel/decel" (A-2) flashes the word "service". Select the letter "U" with the adjustable
knob (1). As a result appearing in the display "rpm/rcf" (A-1) are the words. "Vol=0- 9/Sound". By
pressing the key "rpm/rcf" (4), the desired volume can be adjusted between 0 (low) and 9 (loud) with
the adjustable knob (1), (see Figure 8).
After the settings have been stored successfully, the normal program mode can be changed back
again by switching off the centrifuge for a short period.
Figure 8

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH ZK496_V1.22_eng 17
1.8.5 Song Selection - End of Run
Proceed as illustrated under point 1.8.1 to enter this program mode, press the key "accel/decel" (5).
In the display "accel/decel" (A-2) flashes the word "service". Select the letter "G" with the adjustable
knob (1). As a result appearing in the display "rpm/rcf" (A-1) the word " SonGo/Sound". After
pressing the key „rpm/rcf“ (4) select a song with the adjusting knob (1), (see Figure 9).
After the settings have been stored by successfully, the normal program mode can be changed back
again by switching off the centrifuge for a short period.
Figure 9
1.8.6 Keyboard Sound Turn On / Off
Proceed as illustrated under point 1.8.1 to enter this program mode, press the key "accel/decel" (5).
In the display "accel/decel" (A-2) flashes the word "service". Select the letter "b" with the adjustable
knob (1). As a result appearing in the display "rpm/rcf" (A-1) the word "ON/BEEP". By pressing the
key "rpm/rcf" (4) the keyboard sound (On) or (Off) can be turned on with the adjustable knob (1) (see
Figure 10).
After the settings have been stored successfully, the normal program mode can be changed back
again by switching off the centrifuge for a short period.
Figure 10

PRODUCT DESCRIPTION
18 ZK496_V1.22_eng © Hermle Labortechnik GmbH
1.8.7 Retrieving Operating Data (operated by skilled or service engineer only!)
In the function "Basic Adjustments" retrieving the operating data of the centrifuge is an available
option. Please proceed as illustrated under point 1.8.1. to enter this program mode press the key
"accel/decel" (5). In the display "accel/decel" (A-2) flashes the word "service".
With the adjustable knob (1) the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
S = Software Version
r = Converter Software
E = List of Previous Error Message
h = running time of the motor
The list of the last 99 error messages can be reviewed by pressing the key "rpm/rcf" (4) and scroll
through with the adjustable knob (1). The respective error codes appear in the display "rpm/rcf" (A-
1). Please refer to Table 6: Error Messages (see APPENDIX P.VIII).
Switch off the centrifuge to return to the normal program mode.
Figure 11