Hermle Z 167 m User manual

Operating Manual for

Microlitre Centrifuge

Z 167 M

CONTENTS

1. PRODUCT DESCRIPTION ..................................................................................................1

1.1 Safety Instructions ............................................................................................................................................ 1

1.2 Indended Purpose ............................................................................................................................................. 1

1.3 Brief Description................................................................................................................................................ 1

1.4 Delivery Package ............................................................................................................................................... 1

1.5 Operating and Display Elements...................................................................................................................... 2

1.5.1 LED light......................................................................................................................................................... 2

1.5.2 LCD-Display .................................................................................................................................................... 3

1.6 Signs- and Indication of the Centrifuge........................................................................................................... 3

1.6.1 General............................................................................................................................................................ 3

1.6.2 Product Nameplate (Example) ........................................................................................................................ 4

1.6.3 Warning and Information Signs ....................................................................................................................... 5

1.6.4 Danger, Precautions and Warranty ................................................................................................................. 6

1.6.5 Following Rules Must Strictly be Adhered To:................................................................................................. 6

1.6.6 Warranty.......................................................................................................................................................... 7

1.7 Installation of the Centrifuge ............................................................................................................................ 7

1.7.1 Unpacking the Centrifuge................................................................................................................................ 7

1.7.2 Space Requirements....................................................................................................................................... 7

1.7.3 Installation ....................................................................................................................................................... 7

1.8 Basic Adjustment .............................................................................................................................................. 8

1.8.1 Adjustment of the Rotor Type.......................................................................................................................... 8

1.8.2 Access to the Mode: “Standard settings“......................................................................................................... 8

1.8.3 Signal Turn On / Off ....................................................................................................................................... 9

1.8.4 Keyboard Sound Turn On / Off........................................................................................................................ 9

1.8.5 Sleep Mode On / Off...................................................................................................................................... 10

1.8.6 Retrieving Operation Data............................................................................................................................. 10

2. OPERATING......................................................................................................................11

2.1 Mounting and Loading the Rotor ................................................................................................................... 11

2.1.1 Installation of Rotors...................................................................................................................................... 11

2.1.2 Loading the Angle Rotor................................................................................................................................ 12

2.1.3 Loading and Overloading of Rotors............................................................................................................... 12

2.1.4 Removing the Rotor ...................................................................................................................................... 12

2.2 Power Switch, Fuse......................................................................................................................................... 13

2.3 Lid ..................................................................................................................................................................... 13

2.3.1 Lid Release ................................................................................................................................................... 13

2.3.2 Lid Lock......................................................................................................................................................... 14

2.4 Pre-Selection.................................................................................................................................................... 14

2.4.1 Pre-Selection of Speed and RCF-Value........................................................................................................ 14

2.4.2 Pre-Selection of Running Time...................................................................................................................... 15

2.4.3 Pre-Selection of Brake Intensity and Acceleration......................................................................................... 15

2.5 Radius Correction............................................................................................................................................ 16

2.6 Program............................................................................................................................................................ 17

2.6.1 Storage of Programs ..................................................................................................................................... 17

CONTENTS

II Z167M_V1.18_eng © Hermle Labortechnik GmbH

2.6.2 Recall of Stored Programs ............................................................................................................................ 18

2.6.3 Leaving Program Mode................................................................................................................................. 18

2.7 Starting and Stopping the Centrifuge............................................................................................................ 18

2.7.1 Starting the Centrifuge................................................................................................................................... 18

2.7.2 The “STOP” KEY........................................................................................................................................... 19

2.8 Imbalance Detection........................................................................................................................................ 19

3. MAINTENANCE.................................................................................................................21

3.1 Maintenance and Cleaning.............................................................................................................................. 21

3.1.1 General.......................................................................................................................................................... 21

3.1.2 Cleaning and Disinfection of the Unit ............................................................................................................ 22

3.1.3 Cleaning and Disinfection of the Rotor.......................................................................................................... 22

3.1.4 Disinfection of PP-Rotors .............................................................................................................................. 22

3.1.5 Glass Breakage............................................................................................................................................. 23

3.2 Lifetime of Rotors and Accessories............................................................................................................... 23

4. TROUBLE SHOOTING ......................................................................................................24

4.1 Error Message: Problem / Solution................................................................................................................ 24

4.2 Survey of Possible Error Messages and Solutions ...................................................................................... 24

4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 24

4.2.2 Description of the Error Message System..................................................................................................... 25

5. RECEIPT OF CENTRIFUGES TO REPAIR .......................................................................26

6. TRANSPORT, STORAGE AND DISPOSAL......................................................................27

6.1 Transport.......................................................................................................................................................... 27

6.2 Storage ............................................................................................................................................................. 27

6.3 Disposal............................................................................................................................................................ 27

7. APPENDIX........................................................................................................................... I

EG –Conformity Declaration ................................................................................................................................... II

Table 1: Technical Data ......................................................................................................................................... III

Table 2: Error Messages.........................................................................................................................................IV

Table 3: Radius Correction .....................................................................................................................................IV

Table 4: Symbols- / Abbreviations...........................................................................................................................IV

Redemption Form: Decontamination Certificate ......................................................................................................V

PRODUCT DESCRIPTION

1. PRODUCT DESCRIPTION

1.1 Safety Instructions

This symbol indicates safety instructions and points of potential dangerous situations. Before using

the centrifuge for the first time, please read the operating manual.

Failure to follow these instructions can result in personal injury and/or property damage .

Intended use includes: the observation of all instructions, in the instruction manual, and

administering inspection and maintenance.

1.2 Indended Purpose

This Hermle centrifuge was designed only for the separation of materials or mixtures with different

densities, specifically for the preparation and processing of samples, from the human body, in

context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'

intended purpose. The designated device and its' accessories listed, in the technical documentation,

are in compliance with Directive 98/79/EC for In-Vitro-Diagnostic Medical Devices.

Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.

Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly

prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.

1.3 Brief Description

The unit type Z 167 M is a non refrigerated universal centrifuge, which can be used with 100 –230V

country-specific power cords.

The centrifuge can only be used with one angle rotor.

All parameters are accessible via buttons and selected with the control field. All pre-selected and

current values will be shown permanently on the LCD-display.

The centrifuge is powered by a maintenance-free induction motor.

Detailed technical data are in Table 1: "Technical Data", (see APPENDIX P.III).

1.4 Delivery Package

•1 Centrifuge Z 167 M

•1 Rotor 220.95 V08

•1 Instruction Manual Z 167 A

•1 Power Cord

PRODUCT DESCRIPTION

2 Z167M_V1.18_eng © Hermle Labortechnik GmbH

1.5 Operating and Display Elements

1control field Run Parameters

3LCD Control Panel Display

4rpm/rcf Speed/ g-force

5 accel/decel Acceleration / Deceleration Intensity

6time Centrifugation Time

8quick Short Running

9start Start Centrifugation

10 lid/stop Lid Release / Stop Centrifugation

11 prog Retrieving Stored Programs

12 store Program Store

13 LED light Shows the status of the centrifuge

1.5.1 LED light

The LED light indicates the current operation state of the centrifuge. The following table shows all

operating states.

Color of LED light

Operating state

Green

Run complete, lid is closed

Yellow

Lid opened, no sleep mode

Red flashing fast

Error message

Red flashing slow

Centrifuge runs

Yellow flashing slow

Sleep mode

Red –Green flashing slow

Standard settings menu

PRODUCT DESCRIPTION

1.5.2 LCD-Display

The following picture shows the individual elements of the LCD-Display.

Display Fields:

A-1 Display Field –"rpm/rcf"

A-2 Display Field –"acc/dec"

A-3 Display Field–"time"

Messages/Logo of the Display Field:

M1 „close“ M6 „rcf“

M1 „close“ M8 „decel“

M2 „open“ M9 „radius“

M3 „rotor“ M10 „programm“

M4 Rotor-Nr. M11 „error“

M5 „rpm“ M12 „service“

M6 „rcf“ M13 h m s

M7 „accel“

1.6 Signs- and Indication of the Centrifuge

1.6.1 General

Direction of Rotation –clockwise rotation for the rotor drive

PRODUCT DESCRIPTION

4 Z167M_V1.18_eng © Hermle Labortechnik GmbH

1.6.2 Product Nameplate (Example)

Company Address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen

TYPE: Type Designation of the Product

REF: Article Number of the Product

SN: Serial Number of the Product

Date of Manufacture

Manufacturer

Instructions for Disposal (see chapter 6.3, page 27)

MAX. SPEED: max. Allowed Speed of the Unit

KIN. EN.: max. Kinet Energy with Corresponding Rotor

U/I/f: Allowable Voltage / Max. Current / Frequency

P: Electrical Input Power

Operating Manual

RoHS-Compliance label (see chapter 6, S. 27)

Labeling identifies that all Standards and Guidelines are applied

PRODUCT DESCRIPTION

1.6.3 Warning and Information Signs

Attention! Check the fastening of the rotor nut before each run.

Take off mains plug before opening the housing or the emergency

release.

Power Input

Fuse 2 AT

PRODUCT DESCRIPTION

6 Z167M_V1.18_eng © Hermle Labortechnik GmbH

1.6.4 Danger, Precautions and Warranty

This device may only be operated by a trained professional. Carefully, read the

operating manual and be familiar with the functions of the device.

To protect people and the environment, the following precautions must be taken:

•During centrifugation, the presence of people and the arrangement of hazardous materials is

strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of

EN 61010-2-020.

•The HERMLE Z 167 M is non "explosion-proof" and must not be operated in explosion-

endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such

substances, which chemically react with high energy, is strictly prohibited. If used in such

environment, this is at the users own expense.

•Never spin toxic or pathogenic material without adequate safety precautions, i.e.

centrifugation of tubes with or without defective hermetic sealing, is strictly prohibited. The

user is obliged to perform appropriate disinfection procedures, in case dangerous substances

have contaminated the centrifuge and/or its' accessories. When centrifuging infectious

substances, always pay attention to the General Laboratory Precautions. If necessary,

contact your safety officer!

•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.

•Under no circumstances open the lid of the centrifuge, while the rotor is still running or

rotating with a speed of > 2m/s.

1.6.5 Following Rules Must Strictly be Adhered To:

•Do not operate the centrifuge if not installed correctly.

•Do not operate the centrifuge when dismounted (e.g. without housing).

•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered

with, by unauthorized personnel.

•Do not use accessories such as rotors and adapters, that are not approved by HERMLE

Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or

plastic.

•Do not spin extremely corrosive substances, as they may cause material damages and impair

mechanical resistance.

•Do not operate the centrifuge with rotors or adapters, which show any signs of corrosion or

mechanical damage.

The manufacturer is responsible for safety and reliability, of the centrifuge, only if:

•The unit is operated in accordance to this instruction manual.

•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel

and the electrical installation of the related location corresponds to the IEC-regulations.

PRODUCT DESCRIPTION

1.6.6 Warranty

The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any

manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two

years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage

and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories

or unauthorized modification of the unit.

Technical modification rights are reserved, by the manufacturer, in regards to

technical improvement!

1.7 Installation of the Centrifuge

1.7.1 Unpacking the Centrifuge

Model Z 167 M is supplied in a carton.

Remove the strap retainer, open the carton and remove the centrifuge. The instruction manual must

be kept with the centrifuge at all times!

1.7.2 Space Requirements

The centrifuge should be installed on an even, vibration free and solid surface, if possible on a

laboratory cabinet / table or some other solid vibration free surface.

During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of

30 cm / 12 in on each side of the unit, according to EN 61010-2-020 standards.

Do not place the centrifuge next to a window or a heater where it could be disposed to excessive

heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.

1.7.3 Installation

Follow these steps:

•Check whether the power supply corresponds with the one specified on the manufacturer's

rating label, mounted on the rear panel.

•The line voltage circuit breaker is max. 10 A (Type K), slowly release for commonly used

instruments.

•In case of emergency, there must be an emergency switch off installed outside of the room, in

order to disconnect the power supply from the unit.

•Connect the centrifuge with the mains.

•(The socket for the power cord must be easy to reach, respectively easy to disconnect).

•Switch on, by using the mains power switch (2.2).

•Open the lid, by using the button LID.

•Remove the transport securing device of the motor

PRODUCT DESCRIPTION

8 Z167M_V1.18_eng © Hermle Labortechnik GmbH

1.8 Basic Adjustment

1.8.1 Adjustment of the Rotor Type

For centrifuge Z 167 M, only one rotor (220.95 V08) is acceptable. Due to this, rotor adjustments

cannot be conducted.

1.8.2 Access to the Mode: “Standard settings“

While starting this model, the following basic settings can be performed:

-Signal Turn On / Off

-Keybord Sound On / Off

-Sleep Mode On / Off

The following operating data can be retrieved in this menu:

-Number of Starts

-Operating Hours of Centrifuge

-Operating Hours of Motor

-Software Version

-Error List

-Function of the Imbalance Switch

-Operation of Keyboard

-Hardware Version

-Intermediate Circuit Voltage in Volt

-Display Tests

Open the centrifuge lid and shut off the main switch. Now switch on again the main switch.For

approximately 3 seconds “Soft“ will be shown in the display. Press during this time the keys “time“

(6) and “lid“ (10) simultaneously. As a result, a display test is administered for approx. 3 seconds. All

possible indications will appear at the same time (see Figure 1). The LED light is flashing alternately

with green and red color.

Figure 1

ATTENTION:

-The normal program mode can be changed back again by switching off the centrifuge,

for a short period!

-All changed settings must be confirmed by the key, “start” (9). A confirmation screen

will appear with the word, “store”, in the display “rpm/rcf” (A-1) - Only then the pre-

selections are valid!

PRODUCT DESCRIPTION

1.8.3 Signal Turn On / Off

Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key; "accel/decel"

(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the

control field (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound". By

pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the

control field (1), (see Figure 2).

After the settings have been stored by user, the normal program mode can be changed back again

by switching off the centrifuge, for a short period.

Figure 2

1.8.4 Keyboard Sound Turn On / Off

Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key, "accel/decel"

(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter "b" with the control

field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". After pressing

the key, "rpm/rcf" (4), turn the keyboard sound (On) or (Off), with the rotorary field (1), (see Figure

3).

After the settings have been stored by user, the normal program mode can be changed back again

by switching off the centrifuge, for a short period.

Figure 3

PRODUCT DESCRIPTION

10 Z167M_V1.18_eng © Hermle Labortechnik GmbH

1.8.5 Sleep Mode On / Off

After not using the centrifuge for 5 minutes, the centrifuge automatically falls into sleep mode. The

display is switching off and the LED light is flashing slowly with yellow color. By pressing any bottom,

it switches on again. Proceed as illustrated, under point 1.8.2, to enter this program mode, press the

key, "accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter

"I" with the control field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "SLEEP".

After pressing the key, "rpm/rcf" (4), turn the sleep mode function (On) or (Off), with the rotorary field

(1), (see Figure 3).

After the settings have been stored by user, the normal program mode can be changed back again

by switching off the centrifuge, for a short period.

1.8.6 Retrieving Operation Data

In the function, "Basic Adjustments" the operating data, of the centrifuge, can be retrieved.

Please proceed as described, under point 1.8.2, to enter this program mode.

Press the key "accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".

With the control field (1) the following information can be retrieved:

A = Previous Starts of the Centrifuge

H = Previous Operating Hours

h = Running Time of the Motor

S = Software Version

E = List of Previous Error Messages

F = Function of the Imbalance Sensor

P = Operating of Keyboard

d = Hardware-Version

U = Intermediate Circuit Voltage in Volt

The list of the last 99 error messages can be looked over by pressing the key “rpm/rcf” (4) and leaf

through it with the control field (1). The respective error codes appear in the display “rpm/rc”(A-1)

(see Figure 4). The first two numbers indicate the appeared errors ongoing from 00 to 99, the last

two numbers indicate the error code. Please refer to, "table 2: error messages" (see APPENDIX IV).

Switch off the centrifuge, to return to the normal program mode.

Figure 4

OPERATING

2. OPERATING

2.1 Mounting and Loading the Rotor

2.1.1 Installation of Rotors

Clean the drive shaft and the rotor shaft with a clean, grease-free piece of cloth. Place the rotor onto

the drive shaft, (see Figure 5). Please be sure that the rotor is fully installed onto the motor shaft.

Figure 5

Figure 6

Hold the rotor with one hand and secure the rotor to the shaft, by turning the rotor nut clockwise (see

Figure 6).

ATTENTION:

For safety, always ensure that the rotor nut is tightened before each run!

Do not operate the centrifuge with rotors or adapters that show any signs of corrosion or

mechanical damage.

Do not operate with extremely corrosive substances, which could damage the rotor and the

centrifuge.

OPERATING

2.1.2 Loading the Angle Rotor

Rotors must be loaded symmetrically and with equal weight, (see Figures 7 and 8). The adapter may

only be loaded with the appropriate vessels. The weight differences between the filled vessels

should be kept as low as possible. Therefore, we recommend to weigh with a balance. This reduces

the wear of drive and the acoustic operating noise.

The rotor can be used with a small number of tubes.

Figure 7: WRONG

Figure 8: RIGHT

2.1.3 Loading and Overloading of Rotors

The Maximum Filling Weight and Maximum Speed of the Rotor is:

The maximum load permitted for the rotor is determined by the manufacturer, as well as the

maximum speed allowed for this rotor (see label on rotor), must not be exceeded. The liquid the

rotors are loaded with, should have a max. homogeneous density of 1.2 g/ml or less when the rotor

is running at maximum speed.

In order to spin liquids with a higher density, the speed has to be reduced, according to the following

formula:

Reduced speed nred =√1,2

ℎ𝑖𝑔ℎ𝑒𝑟 𝑑𝑒𝑛𝑠𝑖𝑡𝑦 x max. speed (nmax) of the rotor

Example:

nred = √ 1,2

1,7 x 4.000 = 3.360 rpm

If In case of any questions, please contact the manufacturer!

2.1.4 Removing the Rotor

Untighten the rotor nut completely, counter-clockwise. Lift the rotor vertically out of the centrifuge

(see Figure 6).

Rotor-Number

Max. Speed

Max. Filling

Weight

220.95 V08

14000rpm

43,2g

OPERATING

2.2 Power Switch, Fuse

The power switch and the fuse are located on the back side of the centrifuge (Figure 9).

Figure 9

ATTENTION:

After turning on the power switch, open the centrifuge lid first, before starting the centrifuge.

2.3 Lid

2.3.1 Lid Release

After the run, properly close the lid of the centrifuge, appearing in the display, "rpm/rcf"(A-1) with the

word, "close" (M1). At the same time the actual rotor type, for example “221.57”, is shown in the

display. By pressing the key, "lid" (10), the lid of centrifuge can be released. As soon as the lid is

completely released, the word, "open" (M2) appears. The lid of the centrifuge is now able to be

opened.

For all number marked text, please refer to Figure 10.

Figure 10

In case of defective fuse

open the slider and

exchange the fuse 2.0 AT

OPERATING

2.3.2 Lid Lock

The lid must only be closed slightly. After closing the lid, the word "open" (M2) will no longer be

displayed. As a sign that the centrifuge is ready for starting, appearing in the display, "rpm/rcf"(A-1),

the word "close" (M1). Simultaneously, the word "rotor" (M3) appears, as well as the code number of

the rotor, which is in the centrifuge, “221.57“ (11) along with all rotor specific data, for example: max.

speed, acceleration etc., are available.

For all number marked text, please refer to Figure 10.

ATTENTION:

Before closing the lid, please check if the rotor is tightened.

2.4 Pre-Selection

2.4.1 Pre-Selection of Speed and RCF-Value

By Selecting the key, "rpm/rcf" (4), pre-selection is activated. By pressing the key once, the word

"rpm" (M5) flashes. By pressing the key again, the pre-selection of the centrifugal forces can be

chosen. The flashing word, "rcf" (M6), will appear.

The desired values can be selected, with the control field (1). In the display (A-1), the regulated

value is shown permanently: before, during and after the run.

This pre-selected value will be stored, as long as a new pre-selection is made.

For all number marked text, please refer to Figure 11.

Figure 11

As long as no rotor is inserted, the speed is adjustable between 200 rpm and the maximum

revolution of the centrifuge.

If there is a rotor in the centrifuge, the speed can only be pre-selected up to the maximum

permissible revolution of that rotor. It is the same with the pre-selection of the RCF-Value. The

setting range is between 20 xg and the maximum permissible centrifugal force of the rotor.

The permissible max. speed of the Z 167 M with the rotor 220.95 V08 is 14,000 rpm or 15,994 xg.

ATTENTION:

Please notice the maximum permissible revolutions of the test tubes (Producer Indication)!

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