Hygiena SystemSURE Plus User manual

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Syste m SURE Plus and EnSURE™
Ope r ato r Manual
V5.0

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Th i s M an u a l Co p y r i g h t © 2 0 2 0 b y Hy g i en a LLC
All right s reserv ed. No par t of this m anual m ay be reproduced, st or ed
in a ret rieval syst em , or t ransm it ted, by any m eans or in any for m ,
wit hout t he pr ior perm ission of Hygiena LLC.
The inform at ion cont ained in t his m anual is subj ect t o change w it hout
not ice. Hy giena LLC assum es no r esponsibilit y for any er rors t hat m ay
appear in t his docum ent .
This manual w ill descr ibe t he specificat ions and general operations of
t he Syst em SURE Plus v2 and EnSURE v2 lum inom et ers.
For older lum inom et ers, r efer t o t he oper at or’s m anual t hat shipped
wit h t he lum inom et er for specific oper at ions.
D o cu m e n t : Syst em SURE Oper at or s Man ual
Co py r ig h t , Tr ad em a r k s an d Pa t en t s
Hy giena™, EnSURE™, Ult raSnap™ are t radem arks of
Hy giena, LLC.
A list of Hygiena pat ent s can be found at :
htt ps: / / www .hygiena.com / pat ents.ht m l
Op e r a t i n g Pr e ca u t i o n s a n d Li m i t a t i o n s o f U se
The following sym bol is used in this m anual:
Descr ip tion: CAUTI ON / WARNI NG
I MPORTANT
Hygiena’s products are designed and constr uct ed to be safe
and wit hout risk to healt h when proper ly used in accordance
wit h t he supplied docum ent ation and when t he oper ating
pr ecaut ion s out lined in t his m anual ar e f ully obser ved .
I t is essential t hat the user of this m anual is aware of t he
pot ential hazar ds associated with t he unit and it s accessories
All operat ors should be fam iliar w it h t he safet y precaut ions
and w ar nings given in t his m anual before at tem pt ing t o
operat e t he unit .
I f t he unit is used in a m anner w hich is not specified by t he
m anufact ur er , t he pr ot ect ion p rov ided by t he equipm ent m ay
be impaired.
Please read t his m anual in it s ent iret y and t he “ Operat ing
Precautions and Lim it at ions of Use” at the end of t he m anual
bef or e using t he Lum in om et er .

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Table of Contents
1. INTRODUCTION _____________________________________________________ 1
1.1 WHATISATP?_____________________________________________________ 2
1.2 HOW DOESTHELUMINOMETERWORK?____________________________________ 2
1.3 WHATISAN RLU? __________________________________________________ 2
1.4 TESTDEVICESAND ACCESSORIES _________________________________________ 3
2. LUMINOMETERDESCRIPTION__________________________________________ 4
2.1 KEYPAD SYMBOLS ___________________________________________________ 4
2.2 INSTALLINGBATTERIES________________________________________________ 5
2.3 TURNINGTHELUMINOMETERON AND OFF__________________________________ 6
2.4 DISPLAYLAYOUTAND ICONS____________________________________________ 7
2.5 LOW BATTERYINDICATOR______________________________________________ 7
2.6 POWERSAVINGSTANDBYMODE_________________________________________ 8
2.7 INTERNALCALIBRATION _______________________________________________ 8
2.8 LOW MEMORYWARNING _____________________________________________ 8
3. SET-UPMENU OPTIONS_______________________________________________ 9
3.1 USERID_________________________________________________________10
3.2 SETTINGUPPROGRAM TEST POINTSAND PASS/ FAILLIMITS _____________________ 10
3.3 TESTPLANS ______________________________________________________ 11
3.4 ESTABLISHINGATPPASS/FAILLIMITS ____________________________________ 12
3.5 VIEWINGSTORED TEST RESULTS ________________________________________ 12
3.6 VIEWINGSTATISTICALDATA AND TEST FAILURES _____________________________ 12
3.7 ERASINGTHETESTRESULTSMEMORY ____________________________________ 13
3.8 SETTINGTHECLOCKTIMEANDDATE _____________________________________ 13
3.9 ADJUSTINGTHELCDCONTRAST ________________________________________ 14
3.10 QUICKBOOT ____________________________________________________ 14
3.11 STANDBYMODE __________________________________________________ 14
3.12 BOLD TEXT______________________________________________________ 15
3.13 SAVETESTS _____________________________________________________15
4. SAMPLEMEASUREMENTSAND TEST RESULTS ___________________________ 17
4.1 TAKINGA SAMPLEMEASUREMENT ______________________________________ 18
4.2 PERFORMING A RETEST ______________________________________________ 19
5. INSTALLINGSURETREND DATA ANALYSISSOFTWARE _____________________ 19
5.1 CONDITIONSOFUSE ________________________________________________ 19
5.2 HARDWAREAND SOFTWAREREQUIREMENTS _______________________________ 20
5.3 STARTINGTHEINSTALLPROCESS ________________________________________ 20
5.4 USINGTHESURETREND SOFTWARE ______________________________________ 20
5.5 INSTALLINGTHEUSBSMARTCABLEDRIVER ________________________________ 21

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5.6 CONNECTINGTHELUMINOMETERTO THEPC________________________________ 22
6. OPERATORMAINTENANCE___________________________________________ 22
6.1 CLEANINGTHELUMINOMETERCASE _____________________________________ 22
6.2 REPLACINGTHEBATTERIES ____________________________________________ 22
6.3 CLEANINGAND REPLACING THEPROTECTIVEPOCKET __________________________ 23
7. TROUBLESHOOTING ________________________________________________ 24
7.1 LUMINOMETERBEEPS _______________________________________________ 24
7.2 TROUBLESHOOTINGTIPS _____________________________________________ 24
7.3 LUMINOMETERERRORCODES__________________________________________ 26
8. UNITWARRANTYAND RETURNS ______________________________________ 27
8.1 WARRANTYDURATION ______________________________________________ 27
8.2 PARTICULAREXCLUSION______________________________________________ 27
9. GLOSSARY OFTERMSAND ABBREVIATIONS_____________________________ 27
10. TECHNICALSPECIFICATION __________________________________________ 28
11. CAUTIONSAND WARNINGS _________________________________________ 29

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1 . I n t r o d u ct i o n
The lum inom et er is par t of an ATP Hy giene/ Sanitat ion Monitor ing
Sy st em int en ded t o det ect ATP found in or ganic m at t er an d
m icr oorganism s. The Hygiena lum inom et er , in conj unction wit h
Hy giena’s ATP and Micro- snap t est dev ices, m easur es lev els of
contam inat ion on surf aces, w at er and product sam ples.
1. Rem ov e sw ab f rom t est t ube and collect sam ple. Refer t o
inst ruct ions included with testing device.
2. Place swab back in t est t ube.
3. Break Snap Valve at top of swab by bending bulb. Squeeze
bulb t wice, pushing liquid reagent down swab shaft.
4. Shake test for 5 seconds.
5. Place t est in t he lum inom et er and close lid.
6. Hold t he lum inom et er upright and press “OK” . Reading will
appear in 15 seconds.

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1 .1 W h a t i s A TP?
Adenosine Triphosphate (ATP) is an
organic m olecule t hat is used by living
cells as t he m ain sour ce of energy .
Anim al, plant, bacterial, yeast, and m old
cells produce and break down ATP in or der
t o drive a num ber of biological processes.
I t is t he m ain energy -t ransfer m olecule in
the cell. The presence of ATP on a sur face indicates t hat the surface
has not been adequat ely cleaned and has t he potent ial to harbor and
support bact er ial gr ow th. The residue m ay also contain hazardous
m ater ial including pot ent ial aller gens. Ther efor e, ATP is an ideal
indicator of sur face cleanliness.
1 .2 H o w D o e s t h e Lu m i n o m e t e r W o r k ?
The lum inom et er det ect s ATP using a chem ical react ion.
Lucifer in/ Lucifer ase + ATP = Light
A sam ple is collected using a Hygiena ATP device ( see section 1.4) .
Surface tests com e wit h a buffer m oistened sw ab bud t hat aids in the
r em ov al of an y biolog ical m at er ial ( ATP) on eit her a wet or d ry
sur face. Once t he sam ple is collect ed and exposed t o the chem ist ry
(buffer) in t he Hygiena dev ice, the ATP cont ained inside the collected
cells will be released. ATP released from inside these cells, along with
any free ATP picked up from the surface by t he sw ab, is now available
to react wit h t he chem istry in t he bulb of the device. When t he
Lucifer in/ Luciferase enzy m es ar e ex posed t o ATP, light is produced.
The light is detect ed and m easur ed by the lum inom et er. The am ount
of light det ect ed is dir ectly proport ional to the quantity of ATP
collect ed and accor dingly is propor tional t o t he am ount of residue in
t he t ested sam ple. The higher the reading, t he higher the am ount of
cont am inat ion in t he sam ple.
1 .3 W h a t i s a n RLU ?
The lum inom eter displays results in Relat iv e Light Unit ( RLU) v alues.
The light pr oduced from t he Luciferin/ Luciferase and ATP reaction in
t he sw ab is em it ted in t he form of phot ons. A phot on is an elem ent ar y
part icle and t he basic unit of light . The lum inom et er det ect s t hese
photons and displays t hem directly as RLU values. The m ore light
det ect ed by the lum inom eter , t he gr eat er the RLU value. The
quant it at iv e RLU reading is then com par ed against user
progr am mable t hresholds to provide an over all qualit at ive pass ,
caut ion or fail result.
The lum inom eter detect s total ATP, not j ust ATP from bacteria, yeast ,
and m old but also the ATP from any t hing organic in the sam ple.

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Therefore, an RLU value is not t he sam e as a m icr obial colony form ing
unit ( CFU) . Since t he lum inom et er is det ect ing tot al ATP, it is unk nown
whet her t he RLU result displayed by t he lum inom et er is due t o t he
det ection of m icrobial ATP, residual ATP, or a com binat ion of bot h.
Therefor e, a com parison cannot be dr awn bet ween RLU values fr om
ATP and st andard plate counts ( SPC); or rat her RLU does not equal
CFU. True m icr obial cont am ination can only be det er m ined by a
st andard plat e count m et hod that can t ake up t o 48 hours for result s.
The lum inom et er is capable of providing results in 15 seconds offering
a r apid, real- tim e approach to hygiene/ sanit ation m onit or ing. The
lum inom et er is not m eant t o replace m icr obial t est ing. I t can be an
effect iv e t ool w hen in cor por at ed int o a Hazar d An aly sis Crit ical Con t rol
Point (HACCP) , Good Manufactur ing Pract ices (GMP) , or sanitation
plan.
1 .4 Te st D ev i ce s a n d A cc e ss o r i e s
Device
Cat No.
Desc r iption
Ult r aSnap
US2020
Surface total ATP test device
Supe r Snap
SUS3000
High sensitivity total ATP test suited
for allergen prevention applications
AquaSnap Total
AQ100X
Water total ATP test device
AquaSnap F r ee
AQ100FX
Water Free ATP test device
Conta m ination
Cont r ol Kit
CCK-4
Cuvette based water testing kit
Mic r oSnap
Ente r obacte r iaceae
MS-EB
Enterobacteriaceae test
Mic r oSnap
Colifo r m Test
MS-COLIFORM
Coliform test
Mic r oSnap E. coli
Test
MS-ECOLI
E. coli test
Mic r oSnap Total
MS-TOTAL
Total Viable Count test
Zy m oSnap ALP
ZS
Alkaline Phosphatase test
C r ossCheck
CX3000
Acid Phosphatase test
Calib r ation Kit
PCD4000
Positive and negative rod for
validating luminometer calibration
ATP Positive
Cont r ol Kit
CK25
25 vials of freeze-dried ATP to verify
efficacy of ATP test devices
See t he Hygiena website for a com plet e list of test devices,
accessories, and other products. www.hygiena.com

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2 . Lu m i n o m e t e r D e sc r i p t i o n
Lum inom et er com pon en ts:
2 .1 Key p ad Sy m b ol s
The k eypad is arranged wit h the following buttons:
Power on/ off but t on
Menu select and Exit button
Up/ incr ease but ton
St art/ accept butt on
Down/ decr ease butt on
Program select but t on
St at istics shortcut but ton
The funct ions of t he keypad ar e explained in detail in t he follow ing
sect ions of t his m anual.
TI P: Pressing and holding down the or button will m ake it aut o-
repeat. The longer t he but t ons are held down, the faster it goes.

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Opening t he lid reveals t he following internal feat ures:
RS232 connector cover
Protect ive pocket and
sam ple inser tion por t
For det ails on the RS232/ USB connector and protect ive pocket , refer
to sections 5.6 and 6.1 respect ively.
2 .2 I n st a l l i n g B a t t e r i e s
The unit is designed t o operat e from bot h non-rechargeable alkaline
batt er ies and rechargeable Nick el Met al Hydride ( NiMH) or Nick el
Cadm ium ( NiCD) batter ies:
Ba t t er y
Te ch n o l o g y
N o m i n al
Ce l l V o l t a g e
Re l a t i v e
Cap aci t y
Alk aline
1.5V
1.0
NiMH
1.2V
0.6
NiCD
1.2V
0.5
The unit requires two bat t eries of size AA, LR6 or E91.
TI P: For best result s, always use a quality br and of Alkaline bat t ery
and r eplace as soon as t he bat ter ies becom e low ( see sect ion 2.5).
W A RN I N G: Do not m ix b at t er ies of differ ent t ech nolog ies, or use
recharged alkaline bat t eries as these are prone t o overcharging and
leaking, which m ay cause perm anent unit dam age and m alfunct ion.
The batter ies are fitted by unclipping the bat tery cover on t he back of
the unit , and inserting bot h batt er ies wit h their positive term inals
point ing towar ds t he top of t he unit .
CAUTI ON : Take care not t o insert t he batteries in t he wrong
direct ion, as t his m ay cause per m anent dam age t o t he unit ’s int er nal
electr onics.

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When t he bat teries are inser ted corr ect ly , the unit will aut om at ically
tur n on and enter the clock set - up m ode. (see section 3.8) .
2 .3 Tu r n i n g t h e Lu m in o m e t e r On a n d Of f
To t ur n the unit on, press t he but t on. The unit
will beep once and display the power - up self-
check scr een:
N OTE: I f t he bat teries ar e t oo low, t he unit m ay
t urn on and back off again autom at ically , or m ay
not t ur n on at all. I f t his hap pens, r eplace t he
bat ter ies im m ediat ely.
The unit will then perfor m an int er nal 15 second
calibrat ion (see section 2.7).
N OTE: I f t he clock is not set , the unit will aut om at ically ent er t he t im e
and dat e set - up m ode ( see sect ion 3.8) befor e perfor m ing it s
calibrat ion.
TI P: I t is r ecom m ended you synchr onize t he u nit af ter ch anging t he
batt er ies before use. This will allow SureTrend software t o set the
date, t im e, and for m at to t he com put er ’s dat e, t im e, and form at .
TI P: To act iv ate the backlight feat ures press and hold down t he
but t on. The backlight uses m ore power and will drain the battery
fast er.
To t ur n t he lum inom eter off, pr ess t he but ton. The lum inom et er
will beep once and t he display w ill go blank.
N OTE: To avoid accident al turn off, t he but ton is disabled when
t he lum inom et er is per for m ing a sam ple m easur em ent .

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2 .4 D i sp l a y L ay o u t a n d I c o n s
The liquid cryst al display ( LCD) has the following
layout. The upper half of t he display contains the
st at us icons and t he lar ge RLU result digit s.
The follow ing is a list of icons and their definit ions.
Low bat t ery w arning icon
Lid icon - flashes when t he lid needs to be closed, or t he
RS232 connector cover needs to be properly t ucked under
the lid
I nser t icon – arr ow flashes w hen t he t est ing dev ice should
be insert ed
Rem ov e icon – ar row f lashes w hen a t est ing dev ice need s
t o be r em ov ed
Pass result icon
Caut ion r esult icon
Fail result icon
Sam ple m easur em ent reading in Relat ive Light Unit s ( RLU)
Ret ested icon – identifies t he failed test result as hav ing
been r etested ( see sect ion 4.2)
Ret est result icon – identifies t he Progr am locat ion or test
result as a r etest result
2 .5 Lo w B at t e r y I n d i c at o r
The low bat tery icon indicates t he state of t he bat teries:
I c o n
Ba t t er y St at e
Not visible
Good
Visible
Low – r eplace bat t eries soon
Flashing
Flat – replace batteries now!
When the bat t eries are com pletely low, t he lum inom et er will flash t he
icon, beep t hree t im es, and aut om at ically t urn off.
CA UTI ON : Never leav e low bat t er ies in t he lum inom et er , as t hey
are prone t o leaking/ corrosion, which w ill dam age t he lum inom et er .
TI P: Alw ays store the lum inom et er in a cool dr y place when not in
use because elevat ed tem peratur es will short en t he bat tery life.

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2 .6 Po w e r Sa v i n g St a n d b y M o d e
I f t he lum inom et er is on but has not been used for 19 m inut es, it will
beep every 10 seconds for 1 m inut e, and t hen t urn off. I f any key is
pr essed dur ing t hat t im e t he lum inom et er will r eset t he Standby Mode
count down. The St andby Mode settings can be changed ( see sect ion
3.11) .
To t ur n t he lum inom et er b ack on , sim ply pr ess t he bu t ton .
2 .7 I n t e r n a l Ca l i b r a t i o n
When t he lum inom et er is t urned on ( see sect ion
2.3) , it per form s an int ernal calibr at ion, as t he
display count s down from 15 t o 0 seconds.
N OTE: Dur ing t he calib rat ion sequence, t her e m ust
not be a t est ing dev ice in t he lum inom et er and t he
lid m ust rem ain closed. I f the icon is show n wit h t he arrow flashing,
open t he lid and rem ove t he t est ing device fr om t he lum inom et er. I f
the icon is flashing, close the lid. I f it is still flashing, m ake sur e the
rubber flap covering t he RS232 port is tuck ed under t he lip and is flat .
TI P: The but ton can be pressed at any t im e t o access t he set -up
m enu opt ions ( see sect ion 3) .
When t he calibr at ion is com plete, the lum inom et er is ready to perfor m
a sam ple m easu rem ent .
For det ails of how t o per for m a sam ple m easur em en t
refer t o sect ion 4.
N OTE: When t he lu m in om et er is r eady t o per for m a
m easur em ent , w it h t he lid closed and no t est ing
dev ice present , t he lum inom et er will aut om at ically
perform a recalibr at ion cycle under any of t he follow ing
circum st ances:
• The user presses and holds down t he button for 1 second.
• The lum inom et er is used in an environm ent where t he
t em per at ur e ch an ges significan tly ( t yp ically m or e t han + / - 5oC).
2 .8 Lo w M em o r y W a r n i n g
The lum inom eter can st ore up t o 2000 test result s
in it s int er nal m em ory . When t he m em ory is m ore
than 95% full (i.e. less than 100 test s rem aining)
t he following w ar ning sym bol is displayed.
When t he m em ory becom es com plet ely full, no
additional t est s can be per form ed unt il t he m em or y is eit her er ased
or uploaded to t he PC ( see sect ions 3.6 and 5r espectively). Test can
be perform ed if Save Test is tur ned off ( see section 3.13)

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3 . Set - u p M en u Op t i o n s
With t he lum inom et er tur ned on, t he Set - up Menu can be accessed
by pressing the but ton.
Use the and but t ons to scr oll t hrough t he m enu list , and t hen
pr ess t he but ton to select the r equir ed m enu opt ion, or t he
but t on t he exit t he m enu.
The following 11 m enu opt ions are available:
User I D
Test Plan
Program
St at istics
Mem ory
Clock
LCD Cont rast
Quick Boot
St andby Mode
Bold Text
Save Test s

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N OTE: Sur eTr end soft ware is r equired f or sect ions 3.1 to 3.3. For
furt her det ails, refer t o t he separat e SureTrend User's Manual located
on t he SureTr end softw are CD t hat is packaged w ith t he lum inom et er .
3 .1 Use r I D
The lum inom et er can store a list of 201 User I Ds (User: 0 to 200).
Each t est result will be t agged w ith t he cur rent ly select ed User I D.
Select t he U se r I D m en u opt ion, t hen u se t he and but t on s t o
scroll t hr ough t he list of User I Ds, t hen press t he but ton to select
a User I D, or t he bu tt on t o can cel t he select ion.
TI P: Use r :0 is t he default select ion if no User I D list has been
defined.
3 .2 Se t t i n g u p Pr o g r a m Te st Po i n t s a n d Pa s s/ Fa i l L i m i t s
The lum inom et er has t he ability to store a list of 5001 unique Program
test points (Prog# 0 to 5000), each with its own upper ( ) and lower
( ) pass/ fail result thr eshold values.
Progr am t est point locat ion nam es can only be progr am m ed int o t he
lum inom et er t hrough the SureTrend software. Progr am pass/ fail
thr esholds can eit her be set -up on the lum inom eter, or set using t he
Sur eTrend soft ware. I f a location nam e is ent er ed and t hr eshold set
in SureTrend, and then dow nloaded to the lum inom et er, the threshold
values can’t be changed via t he lum inom et er. This is a security feat ure
and prevent s inadvertent changes to t he t hresholds aft er being set in
SureTrend.
TI P: Pr o g : 0 has a special funct ion, w hereby t he t hr eshold v alues
defined for Pr o g :0 are used as the initial values when set ting-up t he
ot her Pr o g thresholds. Set ting Pr o g :0 wit h your t ypical thr eshold
values w ill sav e t im e w hen set ting- up t he ot her Program s. The
lum inom et er com es w ith pass/ fail t hr esholds of 10 RLU for pass and
30 RLU for fail. These pass/ fail t hr esholds w ere det er m ined based on
a com prehensive st udy of ATP, food r esidue, and m icroorganism
sam ples. To r eceiv e t he r epor t or m or e in for m at ion on set t ing u p
pass/ fail thresholds cont act Hygiena or visit our Quest ion and Answer
section at ww w. hygiena. com .
To set the Progr am result t hresholds on the lum inom et er, fir st select
t he PROGRA M m enu op tion by pr essing . Scroll t o “ PROGRAM” and
pr ess . Then use the and but t ons t o scroll t hrough t he list of
Program s, followed by the but t on t o select t he Progr am to be
changed:

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Pr o g :
Nex t use t he and buttons to first change t he value of t he upper
thr eshold ( ), followed by t he button; to select t he lower t hreshold
use ( ). Use t he and but t ons t o change t he lower value, followed
by the but t on t o store t he new values:
upper threshold ( )
lower t hreshold ( )
N OTE: I f t he select ed Pr ogr am locat ion does not h av e any t hr esh olds
def ined, it m ust be set -up using t he PROGRAM m enu opt ion befor e
the Pr ogram can be used.
3 .3 Te st Pl a n s
The lum inom et er can also store up to 100 individual Test Plans. Each
Test Plan can contain a list of up to 251 t est locations, specified in t he
order in w hich t hey would norm ally be t est ed.
Hav ing select ed t he TEST PLA N m enu opt ion, use t he and
but t ons t o scroll t hrough the list of Test Plans, and t hen press the
but t on t o select t he new Plan, or the button t o cancel t he select ion.
TI P: Pl a n : 0 allow s any of t he 5 001 t est locat ions t o b e t est ed in an y
order , and is the default select ion if no Test Plans have been defined
or select ed.
An exam ple set of Test Plans for a t ypical w eek m ight be:
Pl a n : 1
M o n d a y
Pl a n : 2
Tu e sd ay
Pl a n : 3
W e d n e sd a y
Pl a n : 4
Th u r sd a y
Pl a n : 5
Fr i d a y
Prog: 1
Prog: 1
Prog: 2
Prog: 3
Prog: 4
Prog: 2
Prog: 7
Prog: 8
Prog: 9
Prog: 5
Prog: 3
Prog: 6
Prog: 5
Prog: 4
Prog: 6
Prog: 7
Prog: 10
Prog: 9
Prog: 8
Prog: 8
Prog: 9
Prog: 7
Prog: 6
Prog: 3
Prog: 5
Prog: 2
Prog: 4
Prog: 1
Prog: 10

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Test Plans ar e defined by using t he Su r e Tr en d Windows softw are.
Please refer t o the SureTrend User ’s Manual locat ed on the SureTr end
softw are CD for full det ails ( see section 5) .
To select a Test Plan, use the TEST PLA N m enu
opt ion.
N OTE: I f Test Plan Pl a n : 0 is select ed, or no Test
Plans have been defined, any of t he 5001 Progr am
locations can be select ed.
To select a Progr am location press t he button,
then use t he and but tons to change t he Progr am Pr o g : to the
desired Progr am location, follow ed by t he button t o accept t he new
location, or the button to cancel t he select ion.
TI P: When selecting a Pr o g :, t he display also shows t he t im e and
date t hat t he Program location was last t est ed.
3 .4 Es t a b l i sh i n g A TP Pa s s/ Fa i l L i m i t s
For inst ructions on est ablishing Pass/ Fail lim it s, r efer to t he
im plem ent at ion guide included with your syst em , or visit t he
resources available on w ww. hy giena.com .
3 .5 V i ew i n g St o r e d Te s t Re s u l t s
The lum inom et er can store a m axim um of 2000 t est
r esult s in it s int er nal m em or y, ev en w hen t he
batt eries are low or r em ov ed.
To v iew these st ored t est result s, select the
M EM ORY m enu op tion by pr essing t he but t on . Scr oll t o “ MEMORY”
and press . Then use t he and but t ons t o scroll t hrough t he test
result s, pressing t he but ton to ex it :
TI P: Aft er r eview ing t he st or ed t est results, a failed Pr ogram
location can be select ed for ret esting by pressing t he but ton ( see
section 4.2).
3 .6 V i ew i n g St a t i st i c al D at a a n d Te s t Fa i l u r e s
The t est results dat abase can be analyzed by
select ing t he STATI STI CS m enu option by pressing
t he but ton. Scroll t o “ STATI STI CS” and pr ess .
This will display a sim ple com parison of all the st ored
result s. The but t on also provides a direct shortcut
to t his function.
The and but tons can then be used to scr oll t hrough all of the
failed test results. Press t he button t o exit.

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3 .7 Er a sin g t h e Te st Re su l t s M em o r y
W A RN I N G: On ce t he t est r esult s hav e been er ased
from m em ory they ar e perm anently deleted and can
no longer be viewed or uploaded to Sur eTrend.
N OTE: Once st ar ted, t he er ase funct ion cannot be
st opped, and w ill take about 30 seconds to erase a
full 2000 r esults.
The en tir e t est r esu lt s m em or y can be clear ed u sing t he M EM O RY
ERASE funct ion, by first select ing t he , t hen using the up or down
arrows to select the M EMORY m enu opt ion, t hen press t o select,
and t hen pressing and holding down t he but t on for 2 seconds. The
display will show t he total num ber of stored results to be erased. To
accept and st art t he er ase function, press and hold dow n t he but t on
for 1 second, or pr ess any other button to ex it the opt ion.
3 .8 Set t i n g t h e Clo ck Tim e a n d Dat e
To set or change the clock tim e, dat e, and for m at , select the CLOCK
m enu opt ion by pressing t he but ton. Scroll to “ CLOCK” and press
. Then use t he and but tons t o change t he t im e and date value,
followed by the but t on t o accept each new value.
Fir st the t im e is set ( sty le hour s m inutes) , which can be configured
as eit her a 12- hour clock (select A M or PM ) or a 24- hour clock ( select
2 4 H):
A M , PM or 2 4 H
hours
m inutes
Nex t set t he dat e for m at , w hich can be configured for eit her Eur opean
form at , select D / M / Y for day , m ont h, y ear, or Am er ican for m at ,
select M/ D/ Y for m ont h, day, year) :
D / M / Y or M / D / Y
day ( D/ M/ Y) or month ( M/ D/ Y)
m ont h ( D/ M/ Y) or day (M/ D/ Y)
year
TI P: Pressing the but t on at any point will exit the clock set - up
m ode, leaving t he tim e and dat e unchanged.
N OTE: The clock does not have aut om atic daylight saving
adj ust m ent . I f t his is requir ed, t he t im e m ust be m anually changed
when necessary.

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3 .9 Ad j u s t i n g t h e LCD Co n t r a st
The contrast of the LCD screen is fact ory set at it s opt im um level for
nor m al oper at ing condit ion s. How ev er , in ext r em es of t em p er at ur e
t he display m ay appear too dar k or light .
This set t ing can be m an ually adj ust ed by select in g t he L CD
CON T RA ST m enu opt ion by pressing t he bu tt on. Scr oll t o
“ CONTRAST” and pr ess . Then use t he or but ton t o incr ease or
decrease the contrast level, followed by the but ton t o store the new
set t ing:
darker (+ 1 to + 5 )
Norm al ( 0 )
light er ( - 1 to - 5 )
3 .1 0 Qu i ck B o o t
The lum inom et er w ill t ur n on w hen y ou press t he but t on. The
lum inom et er will perfor m a standard calibration in 15 seconds and w ill
then be r eady to perform readings. Quick Boot can be tur ned off and
t he lum inom et er will perfor m a m or e in- dept h sy stem check t he next
tim e it is turned on. The m ore in- depth syst em check takes 60
seconds.
N OTE: The m or e in - dept h sy st em check is t ypically used f or
diagnost ic purposes and not necessar y for nor m al use.
To change the Quick Boot set t ing, select the QUI CK BOOT m enu
opt ion by pr essing t he bu tt on. Scroll t o “ QUI CK BOOT” and pr ess
but t on t o sw it ch bet ween ON and OFF:
OFF
ON
Ex it
3 .1 1 St a n d b y M o d e
The St andby Mode is used t o control w hen t he lum inom eter should
autom at ically t urn off w hen not in use. This is designed t o conserve
batt er y power.
The default is 20 m inut es. I f no but tons ar e pressed within t he t im e
per iod, t he lum inom et er will t ur n off. I f any but t on is pr essed, or a
reading is perfor m ed, the Standby Mode count down t im er is reset to
zer o.

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I f t he bell icon is enabled t he lum inom et er will begin beeping ever y
10 seconds for 1 m inut e as a warning befor e t ur ning off. For exam ple,
if the St andby Mode t im e is set to 20, then if t he lum inom et er is not
used for 19 m inut es it will st art t o beep every 10 seconds for 1 m inut e.
I f no act ion is t aken t he lum inom et er will t urn off. I f any button ex cept
t he pow er b ut ton is pressed t he count down w ill rest ar t .
To change the Standby Mode setting, select the STANDBY M ODE
m enu opt ion by pressing t he but ton. Scroll to “ STANDBY MODE”
and press but ton. Then use the or but ton to increase or
decrease t he Standby Mode duration, followed by the butt on to
st or e the new sett ing. St andby Mode dur ation can be fr om 5 t o 60
m inutes in incr em ent s of 5 m inut es. Then use t he or but ton t o
enable or disable t he war ning beeps. This will t oggle the bell icon from
enabled to disabled . Pr ess the but ton t o accept the set tings:
incr ease or decr ease dur ation ( 5 to 6 0 )
set duration
enable or disable warning beeps
set war ning st at e
3 .1 2 B o l d Te x t
The lum inom et er display s key inf or m at ion on t he LCD in bold t o
im pr ove legibilit y. I n som e cases the num ber and size of the t ext will
exceed the allotted space on the LCD. I f bold is set t o OFF all text on
the lum inom et er will be displayed norm ally. This will allow all tex t t o
be displayed ev en if t he t ex t is m axim um and all w ide charact er s.
To change t he Bold Tex t set ting, select t he BOLD TEXT m enu option
by pressing t he but ton. Scroll t o “ BOLD TEXT” and press button
to switch between ON and OFF:
OFF
ON
Ex it
3 .1 3 Sav e Te st s
By default any t est perform ed on t he lum inom et er is recorded to the
lum inom et er ’s non- volat ile m em ory. Ev en if you t ake t he bat ter ies out
t he result s of the t est ar e ret ained in t he lum inom et er ’s m em ory .

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I n som e cases, such as tr aining, calibrat ion check s, et c. users m ay
w ant t o discar d t est result s. I f Save Test is set t o OFF, all t est s t aken
in this m ode will not be recorded.
N OTE: The Save Test s m ode will be set back t o ON
when t he lum inom et er is t urned off and back on
again. When Save Test s m ode is set to OFF
“ RESULTS NOT SAVED” w ill appear at t he bot t om of
the LCD
To change the Sav e Test s set t ing, select t he SAV E TESTS m enu
opt ion by pr essing t he but ton. Scr oll t o “ SAVE TESTS” and pr ess
but t on t o switch bet ween ON and OFF:
OFF
ON
Ex it