7
INSTRUCTIONS
KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit
2.2 Precautions and Preparations
The kit provides all reagents required for the PCR. However, in order to achieve reliable
results, the entire assay procedure must be performed under nuclease-free conditions.
Follow the instructions below to avoid nuclease, carry-over, or cross-contamination:
Keep the kit components separate from other reagents in the laboratory.
Use nuclease-free labware (e.g., pipettes, pipette tips, reaction vials).
Wear gloves when performing the assay.
To avoid cross-contamination of samples and reagents, use fresh aerosol barrier
pipette tips.
To avoid carry-over contamination, transfer the required solutions for one experiment
into a fresh tube, rather than directly pipetting from stock solutions.
Physically separate the workplaces for DNA preparation, PCR setup, and PCR to
minimize the risk of carry-over contamination. Use a PCR hood for all pipetting steps.
Sample Material: Use any sample material suitable for PCR in terms of purity,
concentration, and absence of inhibitors.
DNA Extraction: We provide sample preparation kits suitable for all kind of food
samples and primary production stage samples.
Positive Control: Always run a positive control with the samples. Use the provided
control DNA (Control Template) or a positive sample preparation control.
Negative Control: Always run a negative control with the samples. To prepare
a negative control, replace the template DNA with PCR-grade water. Include a
negative control during sample preparation to monitor reaction purity and cross-
contamination. This extraction control can be used as an additional negative control
reaction.
Confirmation
: If required, positive results may be confirmed by appropriate methods
(e.g., reference method).
Waste Disposal:
All contaminated and potentially infectious material, like enrichment
cultures or food samples, should be autoclaved before disposal and eliminated
according to local rules and regulations. For more information, e.g., proper disposal
of unused chemicals, please refer to the appropriate safety data sheet (SDS).
For more information, please refer to the appropriate safety data sheet (SDS). The SDS is
available online at www.bc-diagnostics.com.
Keep the PCR mix away from light and moisture.
