Integra MINI 96 User manual

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Quick Start Guide MINI 96 −96 Channel Pipette
This quick start guide is intended to provide a quick overview of your MINI 96’s
key features and to offer basic instructions for getting started. For detailed
information, please refer to the latest operating instructions that can be found at
www.integra-biosciences.com in different languages.
Use this QR code or visit www.integra-biosciences.com/en/mini-96-getting-
started to access the getting started video.
Intended use
This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD
setting is the sole responsibility of the user. MINI 96 are electronic handheld pipettes that can be
used for aspirating and dispensing aqueous solutions in the volume range of 0.5 μl to 1250 μl using
GRIPTIPS® pipette tips only, see www.integra-biosciences.com.
Safety information
Regardless of the listed safety notes, all locally applicable regulations must be observed.
1) The pipette may only be used by properly trained personnel in a manner specified by INTEGRA
Biosciences.
2) Do not use the instrument near flammable material or in an atmosphere with danger of
explosion.
3) Do not immerse the pipetting unit in liquid. Avoid pipetting of liquids emitting corrosive vapors.
4) Servicing work andrepairs may only be performed by INTEGRA Biosciences or an authorized
after-sales service member.
Getting started
Hold theMINI 96 by thecarrying grip and handle (a, g) and set it up on a perfectly
horizontal surface.
For shipping the instrument, clear the deck, select park head and press OK. The
pipetting unit automatically moves to park position. Use the original packaging.
Insert the adapter cable in the socket (l) and connect the mains adapter to the electricity mains.
Supply voltage: 100 60 Hz– 240 VAC, 50 – .
a. Carrying grip
b. Base stage on deck
c. Magnetic covers, for second stage
d. LED bars
e. Deck light
f. Control unit
g. Carrying handle
h. Pipetting unit, to move up and down
i. AUX port
j. USB-C port
k. Main switch (ON ǀ OFF)
l. DC Input for mains adapter
h
a
c
b
j
k
k
l
g
f
i
e
d

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MINI 96 control unit
m. Display
n. Touch wheel, spin to scroll the cursor
o. OK button, to make a selection
p. RUN button, to start operations
q. Tip ejector
r. PURGE button, to empty tips
s. Arrow buttons, for selections
t. Back button, to navigate backward
Loading GRIPTIPS
•Insert the base stage (b) on the deck. “96” or “384” must match the tip
rack pattern. Put a tip rack on the base stage.
•To load 96 tips four times from racks of 384tips (12.5 μl and 125 μl only),
move the tip rack in one of the four corners, e. g. front left.
•Hold the control unit (f) and lower the pipetting unit (h) down onto the
tip rack until you are prompted to press OK to start automated tip loading.
•Partial tip loading requires the optional two position stage. Press Partial ► and Set No. of
Columns ►. Enter the number of columns filled with tips and press OK.
Setting pipetting height
•To define the lowest possible pipetting height, for ex.
the bottom of a well plate, select Pipetting Height .
•With tips attached move the pipetting unit down to the
desired pipetting height.
•Press Set to save yoursetting.
Running a pipetting program
Select from predefined programs that youcan adapt easily orcreatemulti-stepped customprograms.
Program
Description
Pipet
Liquid transfers when aspirate and dispense volumes are equal.
Repeat Dispense
Dispense multiple aliquots of the same volume.
Sample Dilute
Aspirate two liquids divided by an air gap, followed by dispense.
Pipet/Mix
Multiple mixing by aspiration and dispensing of defined volume.
Manual Pipet
Control the aspiration and dispensing up to the set volume.
Reverse Pipet
Liquid transfers of viscous or high vapor pressure liquids.
Variable Dispense
Dispense multiple aliquots of different volumes.
Multi Aspirate
Aspirate multiple aliquots of different volumes.
Sample Dilute/Mix
Aspirate two liquids divided by an air gap, followed by dispense and mix.
Serial Dilution
Aspirates a transfer volume followed by a mix.
Custom
Creates and store multi-stepped pipetting protocols.
m
n
o
q
s
t
r
p

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Active run step (orange)
Parameters are listed
in the lower half
From the Main Menu, use the
touch wheel to scroll to your
desired program.
Press OK to enter the selected
program.
Insert the GRIPTIPS into the liquid to be transferred.
Press and release RUN to aspirate the volume
selected in the first step.
Your pipette will prompt you to press RUN at each
successive step.
Setting/changing parameters
Press OK to Edit the program.
A list of editablesteps
is displayed.
Select a step and press OK.
Use the touchwheel to set the
value and press OK.
Press to save your settings.
Installation of second stage
The second stage can be used as a second position, e.g., place source
liquids on the base stage and slid a well plate on the second stage for
filling.
•Remove two magnetic covers (c) by using a GRIPTIP.
•Insert the second stage with the desired side (96 or 384 format) facing
upwards.
Two Step Blowout
During aspiration, the piston of your electronic pipette moves up (1).
During dispensing, the piston returns to the initial position (0). During the last
dispense of a program, it automatically moves further down (2) and blows the
remaining liquid from the tip (Blowout).
When the piston returns to the initial position (0), a small amount of air is
aspirated, provided the tip is no longer immersed in the liquid (Blowin).
Note: Manually delay the blowin by holding RUN pressed during the last dispense. Remove the
tips from the vessel and release RUN to start blowin.
Maintenance
Always switch off the device and disconnect from the electricity supply when carrying out
maintenance work.
Clean the MINI 96 housing with a lint-free cloth lightly soaked with mild soap solution in distilled
water or with a 70 % solution of isopropanol or ethanol.
2
0
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The device may be decontaminated with H2O2gas (maximal concentration35 %) for 60 minutes.
If you intend to ship the MINI 96 to be periodically calibrated, the original packaging can be stored
and reused for this purpose. Contact INTEGRA for more information about calibration services.
Equipment disposal
MINI 96 must not be disposed of with unsorted municipal waste. Dispose of MINI 96 in
accordance with the regulations in your area governing disposal of devices.
In certain regions and countries, e.g., in EU member states, the distributor is obliged to
take back this product free of charge at the end of life. Please contact your local distributor
for more details.
Manufacturer and customer service
INTEGRA Biosciences AG
Tardisstrasse 201
CH-7205 Zizers, Switzerland
INTEGRA Biosciences Corp.
22 Friars Drive
Hudson, NH 03051, USA
Find your local representative:
www.integra-biosciences.com
[e-mail]
Direct sales country
Telephone
Mail
China
[phone]
[e-mail]
Denmark, Sweden
[phone]
[e-mail]
France
[phone]
[e-mail]
Germany, Austria
[phone]
[e-mail]
Japan
[phone]
[e-mail]
Switzerland
[phone]
[e-mail]
United Kingdom
[phone]
[e-mail]
United States, Canada
[phone]
[e-mail]
Declaration of conformity
INTEGRA Biosciences AG –7205 Zizers, Switzerland
declares on its own responsibility that the devices
Description
Models
MINI 96
4801, 4802, 4803, 4804
Mains adapter
128909
comply with:
EU directives and regulations
2014/35/EU, 2014/30/EU, 2012/19/EC, 2011/65/EC, 1907/2006, 2019/1782
GBR regulations
S.I. 2016/1101, S.I. 2016/1091, S.I. 2013/3113, S.I. 2012/3032
Zizers, April 11, 2022
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality
For detailed CE declaration and regulations of othercountries, please refer to the operating
instructions.