Integra VACUSIP User manual

VACUSIP Operating instructions
159950_V00

Declaration of conformity | Konformitätserklärung |
Déclaration de conformité | Declaración de conformidad |
Dichiarazione di conformità
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the product | erklärt in alleiniger Verantwortung,
dass das Produkt | déclare sous sa responsabilité exclusive, que le produit |
declara bajo su propia responsabilidad que el producto | dichiara sotto la propria respon-
sabilità che il prodotto
in accordance with EC directives | gemäss der EU-Richtlinien | est conforme au terme de
la directives CE | de acuerdo con las directivas CE | in conformità alle direttive CE
is in compliance with the following normative documents: | mit den folgenden normativen
Dokumenten übereinstimmt: | aux documents normatifs ci-après: | cumple las
documentos normativos: | soddisfa le normative seguenti:
Standards for Canada and USA
Zizers, 10.1.2011
VACUSIP Models: 159 000, 159 010
2006/95/EC Low Voltage Equipment
2004/108/EC Electromagnetic compatibility
2002/95/EC Restriction of Hazardous Substances
2002/96/EC Waste Electrical and Electronic Equipment
2009/129/EC Eco Design Directive
EN 61010-1 Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61326-1 Electrical equipment for measurement, control and laboratory
use - EMC requirements.
UN 38.3 Lithium Battery Testing 4th edition (model 159 000 only)
CAN/CSA-C22.2
No. 61010-1 Safety requirements for electrical equipment for measurement, control and laboratory use - General requirements.
UL Std. No. 61010-1 Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
FCC, Part 15, Class A Emission
Elmar Morscher
CEO Thomas Neher Quality Manager

VACUSIP – Operating instructions V00 Introduction
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Table of Contents
1 Introduction ................................................................................................................3
2 Description of the device ............................................................................................4
3 Installation ..................................................................................................................5
4 Operation ...................................................................................................................6
5 Maintenance ...............................................................................................................9
6 Technical data ..........................................................................................................10
7 Accessories and consumables .................................................................................12
Imprint
© 2011 INTEGRA Biosciences AG
Manufacturer
INTEGRA Biosciences AG
CH-7205 Zizers
Switzerland
www.integra-biosciences.com
[e-mail]
Customer service
Please contact your local INTEGRA Biosciences AG representative, see
www.integra-biosciences.com or contact [email protected].
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the VACUSIP aspiration system.
The VACUSIP is a vacuum-based system for aspiration of non explosive aqueous
solutions, such as biological buffers and media. It is not intended for medical use.
1.1 Safety notes
WARNING
Read these operating instructions carefully before use and pay particular
attention to sections containing this symbol.
1.1.1 General
Do not open or modify the VACUSIP in any way.

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1.1.2 Battery (model 159 000)
Charge the VACUSIP device using only the charger supplied by INTEGRA Biosciences.
The battery may not be replaced or removed by the user. Do not heat the lithium-ion
battery or VACUSIP above 60 °C (140 °F).
If despite all safety measures the VACUSIP lithium-ion battery catches fire and toxic
(typically dense, white) fumes emanate from the VACUSIP device, immediately evacuate
the working area. The VACUSIP housing is made of special flame retardant material that
has proven its capability to contain a battery fire. During a battery fire the housing surface
may become very hot. Do not touch the housing by hand and wait at least 20 minutes after
fumes have disappeared before approaching the device. Do no longer use the VACUSIP
device and contact your supplier for safe disposal.
2 Description of the device
Scope of delivery
• VACUSIP base unit without battery (model 159 010) or with battery (model 159 000)
• Mains adapter
• INTEGRA glass bottle 500 ml with standard GL 45 lid
• GL 45 lid with tube fittings
• Tubing and filter with an additional spare filter
• VACUSIP hand operator
• 8-channel stainless steel tips
• 1-channel stainless steel tip
• 1-channel adapter for disposable tips
1 On/off switch
2 Indicator LED
3 Housing
4 Stainless steel rod
5 VACUSIP hand operator
6 Hand operator mount
7 Lid with tube fittings
8 500 ml bottle for liquid collection
1
3
2
4
5
6
7
8

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Rear view:
3 Installation
3.1 Power supply
Insert the mains adapter cable into the VACUSIP mains connection socket (13) and plug
it into the power source.
WARNING
Use an original INTEGRA Biosciences mains adapter only (see
“Specifications” on page 10 for voltage requirements)
3.2 Charging of VACUSIP battery model
A full charge takes 5 hours but before the first use, VACUSIP should be charged for at
least 10 hours.
When the LED (2) starts to blink green, the battery needs to be recharged immediately to
prolong battery life. VACUSIP has an integrated overcharge protection.
The VACUSIP can be used while it is being charged.
3.3 Set-up
3.3.1 Installing the VACUSIP hand operator mount
Find the enclosed stainless steel rod (4) and push it into the opening (12).
Attach the hand operator mount (6) to the top of the stainless steel rod (4).
WARNING
Do not carry the VACUSIP by holding the rod or the hand operator mount.
Always grab the instrument at the back to carry it around.
9Filter
10 Silicone filter adapter
11 Air outlet
12 Opening to insert stainless steel rod
13 Mains connection socket
13
9
10
11
12

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3.3.2 Assembling the hand operator
3.3.3 Connecting the filter
Attach the shorter silicone tubing to the filter (9) and then plug the filter into the silicone
filter adapter with the blue side facing the instrument.
The VACUSIP should never be used without a filter.
3.3.4 Connecting the silicone tubing
Place the bottle in the instrument and screw on the green lid with tube fittings (7).
Attach the silicone tubing coming from the filter to the fitting on the lid named
“FILTER”.
Attach the longer silicone tubing to the fitting in the lid named “LIQ” (liquid) and the other
end to the VACUSIP hand operator (5).
4 Operation
4.1 Vacuum activation and notes on use
Switch on the VACUSIP to start the vacuum pump (the LED lights up green).
Once the vacuum is reached, the pump stops until the vacuum falls below threshold.
VACUSIP switches off automatically after the pump has not been running for 10 minutes
or 40 minutes after initial switch-on. This feature can be temporarily deactivated by
pressing the power button for at least 4 seconds when switching on the instrument. The
power-off function is automatically restored after turning off the VACUSIP.
Check the level of liquid in the bottle frequently. The liquid level should never reach the lid.
Insert the innner part in the
silicone cover, as shown on the
picture beside.
Attach a suitable adapter to the hand operator.
To aspirate press the soft buttons on both sides of the
VACUSIP hand operator.

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Prevent liquid or foam overflow from the collection bottle into the filter. In case the filter
gets accidently wetted or soiled, exchange it immediately or otherwise the retention of
further liquid cannot be guaranteed. Note that the hydrophobic filter holds back aqueous
solutions, whereas retention of apolar liquids is limited.
WARNING
When working with dangerous substances, you must comply with the material
safety data sheet (MSDS) and the additionally applicable regulations of trade
associations, health authorities, supervisory offices, etc. In addition, when
working with hazardous volatile substances or biohazardous agents, either
place the VACUSIP inside a biosafety cabinet or connect the instrument's air
outlet (11) to a safety ventilation by means of a tube (ID=4 mm) and an
aspiration needle (Part. No. 155 502).
4.2 Code of indicator LED
LED code Meaning
Constantly green Unit is powered on and no errors occurred.
Blinking green Battery level low.
Blinking red once per second Leakage detection. Pump turns off if end vacuum
has not been reached after 5 minutes.
Blinking red 5 times per second Vacuum generation is too fast (~100 mbar/s).
Indicates a clogged part.
Constantly red Battery error. Press the power button to continue
using the mains power supply.
Blinking red and green Error in vacuum sensor calibration. Please contact
your supplier.

VACUSIP – Operating instructions V00 Operation 8 INTEGRA Biosciences AG
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4.3 Troubleshooting
Problem Probable cause Remedy
Device is not running. No electrical power. Check if mains adapter cable is plugged in or if battery
(model 159000) is discharged. Check indicator LED light.
Unit automatically switched off. Switch on the unit (LED lights up green).
VACUSIP automatically switches off if it is not used or 40
minutes after initial switch-on.
Aspiration speed is low. Leak in the vacuum system. Close the bottle lid tightly and attach tubing firmly. Check
tubing, filter and hand operator for leaks. If the maximum
vacuum is no reached for 5 min, the pump is turned off.
Liquid overflowed and blocked the
filter. Empty bottle and exchange filter.
Tubing is blocked. Clean or exchange the tubing.
Hand operator is clogged. Take apart the hand operator to clean it. Use an ultrasonic
bath to clean it of debris.
Replace the hand operator.
VACUSIP turns off after
a few seconds or
minutes.
Clogging of a part close to the
pump. The LED blinks red rapidly. Check if filter is blocked and exchange it, if necessary.
Check if the filter adapter is blocked.
Leak in the vacuum system. The LED blinks red slowly.
The pump automatically stops if the end vacuum is not
reached after 5 min. Check for leaks in the system and push
the power button again.

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5 Maintenance
5.1 Cleaning and servicing
To clean the VACUSIP housing use a cloth moistened with soapy water or a 70% ethanol
solution.
It is recommended to change the hydrophobic filter regularly, at least once a year.
Exchange the filter immediately if liquid has entered.
When working with solvents and other hazardous materials always rinse the hand
operator and tubing with water followed by 70% Ethanol.
5.2 Autoclaving and chemical sterilization
5.2.1 Autoclaving
All parts contacting the liquid can be autoclaved: 500 ml glass bottle, lid with tube fittings,
silicone tubing and hand operator. The hand operator should be taken apart for optimal
decontamination. To disassemble it, hold the silicone cover and pull out the inner part.
Silicone may become brittle after extensive autoclaving. Replace the tubings and the hand
operator if they are damaged.
WARNING
The filter and the instrument itself cannot be autoclaved.
5.2.2 Chemical sterilization
Use disinfectants that are free of chlorine or other corrosive agents, especially when
corrosive vapors are generated during aspiration. Add the disinfecting agent to a filled
bottle (and not to the empty bottle) and let it stand for an appropriate amount of time.
5.3 Equipment disposal
The VACUSIP device must not be disposed of with unsorted municipal waste.
The VACUSIP (159000) contains a polymer lithium-ion battery. Do not dispose
of the device in a fire. Dispose of the VACUSIP device in accordance with the
laws and regulations in your area governing disposal of devices containing
lithium-ion batteries.

VACUSIP – Operating instructions V00 Technical data
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6 Technical data
6.1 Specifications
6.2 Chemical compatibility
The table below lists VACUSIP parts that come into contact with the aspirated liquid or its
aerosols and vapors, and rates the compatibility of these parts to a few of the chemicals
commonly used in laboratories. To determine the compatibility of a component to a
chemical not listed in the table, please consult one of the several tables available on the
internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.
INTEGRA Biosciences AG does not warrant that the information in the table is accurate
or complete and that any material is suitable for any purpose.
Vacuum range –250 mbar +/- 20%
Flow rate (air) Pump: 1.5 l/min +/- 30% at ambient pressure
Dimensions
(H x W x D) 125 x 120 x 162 mm (unit) Height: unit with bottle and hand operator mount 345 mm
Weight Unit only: 500 g
Unit complete: 970 g (with glass bottle, tubing and hand operator)
Mains adapter 100–240 VAC, 50/60 Hz
9 VDC / 0.5 A
Battery Li-Ion, 9V
Degree of protection IP 31
Ambient conditions Altitude: up to 2000 m
Temperature: 5 – 40 °C
Humidity: max. rel. humidity 80% for temperatures up to 31 °C
decreasing linearly to 50% rel. humidity at 40 °C.

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Chemical compatibility chart
Compatibility ratings:
G = Good: no or minor effects.
F = Fair: moderate effects, not recommended for continuous use.
C = Critical: not recommended, suitability to be determined by test.
Parts Materials
JAVEL (e.g. NaClO) Acetic acid EtOH Isopropyl alcohol NaCl Sodium hydroxide Sodium acetate
(3M, pH 5.2)
Hydrochloric acid (20 %) Chloroform Acetone Ammonium acetate Nitric acid (20 %) DMSO Phenol
Hand operator Inner part PP G G G G G G G G F G GGGG
Outer part Silicone G F G G G G C G C C NA C G C
Adapters POM C C G G G G G F G G G C G G
Tubing Silicone G F G G G G C G C C NA C G C
Bottle Single-use PP GGGGGG G GFGGGGG
Lids PP GGGGGG G GFGGGGG
Gasket EPDM GGGGGG G GCGGGGG
Filter Membrane PTFE GGGGGG G GGGGGGG
Housing PVC GCFGGG G GCCGGCF
Filter adapter Silicone G F G G G G C G C C NA C G C
VACUSIPbaseHousing PP GGGGGG G GFGGGGG
Pump housing POM C C G G G G G F G G G C G G
Pump membrane NBR G F F G G G G G C C G C F C

VACUSIP – Operating instructions V00 Accessories and consumables
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7 Accessories and consumables
Accessories Part No.
Adapter 1-channel stainless steel tip 40 mm 155 502
1-channel stainless steel tip 280 mm 155 525
4-channel stainless steel tip 40 mm 155 524
8-channel stainless steel tips 40 mm 155 503
1-channel adapter for disposable tips/GripTips
(pack of 5) 159 023
1-channel adapter with ejector for disposable tips 159 026
8-channel adapter with ejector for disposable tips 159 024
1-channel adapter with ejector for GripTips 159 027
8-channel adapter with ejector for GripTips 159 025
Stainless steel rod For holding the hand operator mount 159 045
VACUSIP hand
operator mount To park the VACUSIP hand operator 159 046
Mains adapter
(100 - 240 VAC,
50/60 Hz)
EU 156 631
US 156 630
JP 156 634
UK 156 632
AU 156 633
Consumables Part No.
Filter For protection of the pump, non sterile, 0.45 µm 153 016
Tubing Silicone, 3 mm ID, 2.5 m 171 023
Silicone, 3 mm ID, 25 m bulk roll 171 033
Tubing set
VACUSIP Consists of: VACUSIP Hand operator, Silicone tubing 1.2 m, Silicone tubing 0.22 m
1 filter (non-sterile, 0.45 µm)
159 040
Bottle INTEGRA borosilicate bottle with standard GL 45 blue
lid, 500 ml 159 031
Single-use PP bottle with GL 45 lid, 500 ml (pack of 10) 159 032
Lid GL 45 with tube fittings (pack of 2) 159 035
Hand operator
VACUSIP To operate VACUSIP and attach adapters 159 020