Labnet MultiGene Mini User manual

MultiGeneMini
ThermalCycler
TC020-24 /TC020-24-230V
TC050-18 /TC050-18-230V
Operation Manual
Ver.1.2
Labnet International

CONTENTS
CHAPTER 1 SPECIFICATIONS ...................................................................................................... 1
1 Normal Operating Conditions .................................................................................................. 1
2 Transportation and Storage Conditions................................................................................... 1
3 Basic Specifications................................................................................................................. 1
4 Performance ............................................................................................................................ 2
5 Software Functions .................................................................................................................. 2
CHAPTER 2 Features ........................................................................................................................ 3
1 Description ............................................................................................................................... 3
2 Keyboard Diagram................................................................................................................... 4
3 Key Functions .......................................................................................................................... 4
4 Inspection before Power-up..................................................................................................... 5
5 Power-on Procedures .............................................................................................................. 5
CHAPTER 3 OPERATION GUIDE..................................................................................................... 6
1 How to Run a Program ............................................................................................................ 6
1.1 How to edit a program....................................................................................................... 7
1.2 How to Set System Parameters...................................................................................... 12
CHAPTER 4 TROUBLE SHOOTING............................................................................................... 14
1 Failure Analysis and Processing Procedures........................................................................ 14
2 Block Error Messages ........................................................................................................... 15
APPENDIX 1 Wiring Diagrams .................................................................................................... 16
APPENDIX 2 Parameter Values.................................................................................................. 18

1
CHAPTER 1 SPECIFICATIONS
This chapter describes the MultiGene Mini Thermal Cycler’s operation, transportation
and storage conditions, basic parameters, performance and functions.
1 Normal Operating Conditions
Ambient operating temperature: 10°C ∼30°C
Relative humidity: ≤70%
Power supply: ~100-120V, 200W, 50/60Hz
~220-240V, 200W, 50/60Hz
Note:
Before powering on, please confirm that the above operating
conditions are satisfied. The unit must be plugged into a
grounded outlet.
2 Transportation and Storage Conditions
Ambient temperature: -20 ºC～+55 ºC
Relative humidity: ≤80%
3 Basic Specifications
Model
Parameters
TC050-18
TC020-24
Sample Capacity
18
24
Tube Volume (ml)
0.5
0.2
Dimensions (mm)
297×212×200（L×W×H）
Display Screen
320×240 Backlit LCD
Weight (kg)
3.2
250V 2.5A (Ф5×20mm)
Fuse
125V 5A (Ф5×20mm)
Computer
Interface
RS232

2
4 Specifications
Temperature range: 4ºC～99ºC
Maximum Heating rate: ≥5.0ºC/sec(max)
Maximum Cooling rate: ≥4.0ºC/sec(max)
Temperature Resolution ± 0.1°C
Block Temperature accuracy: ≤±0.3ºC
Temperature display accuracy: ≤±0.5ºC
Block temperature uniformity: ≤±0.5ºC
Heated lid work temperature range: 105ºC±5ºC
Note: These performance specifications can be met when the unit is operated in Ambient
temperature: 23±5ºC,humidity≤70%
Temperature control accuracy and block temperature uniformity are tested at the following
temperature settings: 55ºC ， 72ºC ， 95ºC
Max. number of cycles: 99
Max. Segments within a cycle: 9
Max. temperature time: 99 min. 59 sec.
Max. incubation and in situ time: 99 hour 59 min.
Program storage capacity: 100
5Software Functions
File editing and saving
File accessing, modifying and deleting
Automatic file link
Heating/cooling rate setting
Automatic temperature and time increments/decrements during cycling
Real-time display of data at each phase of program execution
Pause
Stop
Auto-restart in case of power failure
Audible alarm
Run time estimation
Remaining time estimate
Date and time (year, month, day, hour, minute, second) display
Audible alarm in the event of failure
Note:
The above software functions are listed for reference only. Labnet
reserves the right to modify the software without notice.

3
CHAPTER 2 Features
This chapter covers the MultiGene Mini Thermal Cycler’s mechanics, the keyboard
and each key’s function, and preparations before power-on.
1 Description
①Heated lid & Block
②LCD Display
③Keyboard
④RS232 interface
⑤Power switch
⑥Power connecter
⑦Fuse Socket
Note:
1 The fuse specifications are as follows: 250V:2.5A / 125V:5A, Ø5×20mm. The fuse
should be replaced with one that meets these specifications.
2 Contact your distributor or Labnet’s service department if you need proper fuses.
4
3
2
1
7
6
5

4
2 Keyboard Diagram
3 Key Functions
“CE”，can be used to change “+, -” when editing
temperature and time.
“Right/Left”，moves the cursor one position to
the left or right during file parameter setting.
“Up/Down”，moving the cursor one position up or
down during file parameter setting.
“Enter”，press it to accept the present parameter
settings displayed on the screen.
“Function”, press any button to accept the
current items displayed above the buttons.
“Numbers & Letters”,
select the letter by pressing
the key until your selection
appears on the screen.

5
4 Inspection before Power-up
Before switching the power on, please ensure that:
1) Supply voltage matches the voltage rating label on the instrument. (refer to
Chapter 2);
2) The plug has been inserted into the power socket;
5 Power-on Procedures
Turn on the power (i.e. turn the power switch to position ‘1’), the MultiGene Mini
Thermal Cycler will beep 4 times, and the LCD will display ‘ ’. This
means the device is self-testing, which takes about 1-2 minutes. After the self-test is
complete, the main menu will be displayed, and the device is ready for the following
operations, editing, accessing, modifying, or deleting a file.

6
CHAPTER 3 OPERATION GUIDE
There are eight menu selections for the MultiGene Mini Thermal Cycler. In this
chapter, we will explain operation, for example, how to edit, access, modify, delete,
or run a protocol file and how to set parameters.
Warning!
If an abnormal sound or display appears on start up or if a failure error
during the Self Test mode, Turn off the power immediately and contact
Labnet or your distributor
Note:
If the number of samples is less than the number of wells in the block,
the sample tubes should be evenly distributed over the block, in order
to ensure that the heated lid will evenly press on the tops of the sample
tubes. This also ensures that both the block’s load and the temperature
distribution to each tube are even.
Note:
Before closing the cover, turn the knob counter-clockwise according to
the knob diagram until there is a "click....." Please note that the knob
can be tightened by turning it clockwise, After closing the cover but
before power on, turn the knob clockwise until you hear a click, then
turn the knob one more turn.
When the knob is turned clockwise or counter-clockwise, you will hear a
"click" sound, To open pull the release latch with your finger; and turn
the knob counter-clockwise.
1How to Run a Program
Switch on the power, the MultiGene Mini Thermal Cycler will beep 4 times. The
screen will display “ ”，and the instrument will carry on self test.
The self test takes about 1~2 minutes. If the self test is successful, the screen will
display the main Menu.
On the left top corner of the main
screen the Hot lid status is displayed.
The center of the screen displays
Labnet, the software version number,
block model, default file, default user,
control mode and sample volume.
Press “File” to enter file list
interface.
Press “System” to enter system
parameter setting menu.
Main Screen
Hotlid=020C 2009-04-01 10:05:00
Labnet
MultiGene Mini
Version: 2001-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
File System Run n

7
Hotlid=020C 2009-03-24 10:05:00
Labnet
MultiGene Mini
Version: 2009-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
Back Run n
If the default user does not have a password, press “Run” to run the default file. You
will be prompted to enter sample volume(if the unit is set for Tube Mode) in the pop
up window. If the unit is set for block mode, the unit will begin running the default file.
If the default user does have a password, you will be prompted to enter it before you
can run the default file.
Password Prompt
Use the keypad to enter the
password. Press “Back” to go back
the main menu. Press “Accept” to
confirm the password and enter file
running interface or sample volume
input window. If the password is
incorrect, a dialog box will be
displayed as below:
If the current Control Mode is Block
mode, press “Accept” button to
enter file running interface. If the
current Control Mode is Tube mode,
press “Accept” button, a dialog box
will be displayed to input the sample
volume.
Enter sample volume used. By using
Tube mode, the temperature
overshoot and time will be adjusted
for different sample volumes.
Press “Back” to go back the main menu.
Press “Run” to enter the file running menu.
1.1 How to Edit a Program
Press F1 “File” on the main screen to enter the file list menu. There are 3 files listed
as shown in the image. When the flashing bar is in the User Column, select users
with the up/down keys; The File Name Column will display the files of the selected
Hotlid=off 2009-04-01 10:05:00
Labnet
MultiGene Mini
Version: 2009-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
Back Acceptt
Enter password
-
Please enter sample volume:
020µl
Please enter sample volume:
020µl
Password error. Try again.
-
Enter password
-

8
user. Shift to the File Name Column, using the “right” arrow button, then select the
user’s file using the up/down keys.
Press “Edit” to edit the file
indicated by the cursor.
Press “New File” to create a new
file.
Press “Delete” and you will be
prompted “Do you want to delete
the file? Please select ‘F3’or
‘F4’”, Select（Delete）or （Back）.
Press “Back” to go back to the
main menu.
If the file has no password, press
“Edit” to enter file editing interface;
If the file has a password, press
“Edit”, a dialog will be displayed to
indicate the user to enter the
password.
Enter the password. Press
“Accept” to confirm the password,
and the system will enter the file
editing menu; or press “Back” to go
back to the file list interface.
If the file has no password, press
“Run” to enter file run menu or enter
the sample volume. If the file is
password protected, press “Run”, a dialog box will be displayed to input the
password. Press “Back” to go back
the file list interface. Press “Accept”
to confirm the password.
After pressing “Run” in the file list
interface, and entering the
password (If Password protected):
If the current Control Mode is Block
mode, Press “Accept” to enter the
file running interface; If the current
Control Mode is Tube mode, press
“Accept”, and a dialog box will be
displayed to input sample volume.
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
AMP1
2007-07-28
2
Mike2
AMP2*
2007-07-28
3
Sue3
AMP3
2007-07-28
Back Accept t
Enter password
-
Please enter sample volume:
020µl
Enter password
-
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
Amp1
2008-07-28
2
Mike2
Amp2*
2008-07-28
3
Sue3
Amp3
2008-07-28
Edit New File Delete Back Run n
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
AMP1
2008-07-28
2
Mike2
AMP2*
2008-07-28
3
Sue3
AMP3
2008-07-28
Back Run n
Please enter sample volume:
020µl
File List Menu

9
Hotlid=020C 2008-11-01 10:05:00
100
75
50
25
0
Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0
Time 01:00 02:00 01:45 00:00
Seg. Temp(C) Time Ramp(C/s) +Temp +Time
1c 95.0 01:00 #.# +0.0 +0:00
2c 55.0 02:00 #.# +0.0 +0:00
3c 72.0 01:45 #.# +0.0 +0:00
4 04.0 00:00 #.# +0.0 +0:00
Cycle1：×10 From 01 to 03
+Seg. +Cycler Delete Back Save/Run
Use the key pad to input the sample volume. Press “Back” to go back to the
file list menu. Press “Run” to enter file running menu.
Note：
The symbol “*”at the top of User and File indicates the default User and
file. The Default User name is LABNET and the default file is 1. The
default file cannot be renamed, but it can be edited.
File Editing Screen
In this screen, you can add and edit
cycles and segments of the file. A File
is comprised by cycles and segments.
The cycles contain the segments,
which are repeated. Move the cursor
by pressing “Up/Down”and “Right
/Left”. Press the keys to modify
parameter settings. Press “CE”to
change +/-.
Press “+Seg.” to add a segment.
In every segment, you can set
temperature (Temp), duration
(Time), Ramping rate (Ramp),
temperature increment of every
cycle (+Temp) and time increment
of every cycle (+Time). Press “Delete” to delete the present segment.
NOTE: setting ramp to #.# provides the maximum ramping rate.
Press “+Cycle” to set cycle number and choose cycle segments to be included
in the cycle. The figure shows a cycle including the first segment to the third one,
and the cycle is set to repeat 10 times (x10).
Press “+Cycle” repeatedly to add cycles (max.: 5 sections ). Press “Delete” to
delete the present segment. Move cursor by pressing “Up/Down” to enter the
former segment or the latter.
Press “Save”to enter the file save menu.
Press “Back” to go back to the File List menu.
Please Note:
1. After editing a program, save it first before running.
2. When setting “Time” as “--:--” (i.e. ∞), the hot lid will automatically turn off
when the block reaches the set temperature.

10
File Saving Screen
In this screen use the keypad to enter
the file name. Press “Enter”to move
the cursor down one position. Select
User and File Name through the pop-
up character selection box. Click left
button to delete characters while
clicking right button to move the
cursor. Press the “Caps” key to shift
from a capital to lowercase letter, and
on the top left a capital or small letter
indication will appear. (example: abc)
Press “Main” to go back to the
main interface.
Press “Save”to save the file. When the file is normally saved, “File has been
saved. Please continue another operation” will appear. Press “Enter” to close
the reminder window. If the file name was already in use, the system will
display（Rename）or continue to save (Save).
If the current Control Mode is Block mode, press “Run” to enter the file running
interface; if the current Control Mode is Tube mode, pressing “Run” will open, a
dialog box to input the sample volume.
Relation of cycle and segments in a file
File
section 1
Cycle1 ×** From ** to **
Seg. 1
Seg. 2
Seg. 16
section 2
Cycle2 ×** From ** to **
·
·
·
·
·
·
section 5
Cycle5 ×** From ** to **
File Save Interface
Hotlid=off 2009-07-28 10:05:00
abc
User：LabnetI I
File Name：1 1
Password：***** *
Password confirm：***** *
Main Caps Save Run n
Please enter sample volume:
050µl

11
Enter the sample volume. Press “Back” to go back to the file save menu. Press
“Run” to start running the file.
In the file save menu, if you press “Run” without pressing “Save”, the system will
display “The modifications will be lost
after running. Do you still want to
run?”
Press “No” to go back file save
interface.
Press “Yes” to enter the file
running menu and sample volume
input screen. (Note: the
modification here cannot be
saved after the program is run.
When the file is running, “Now
Running•••” will continuously flash.
When the protocol is complete, “File
running is over is displayed, Press
‘Enter’ to return to the main menu.”
Press “Stop” and “Do you want
to stop running?” will be
displayed. Select ‘F1’or ‘F3’”.You
can then select either “Stop” or
“Start”.
Press “Pause” and the display
will show “Pause state now.
Press ‘F3’ to continue running.”
You can then select “Start” to
continue.
Press “View File” to view the file in the editing format.
Note: the file cannot be edited while running
Press “Skip” to stop current temperature segment and run the next segment.
Hotlid=off 2007-07-28 10:05:00
abc
User：Labnet i
File Name：Num1 1
Password：Num1 1
Password confirm：Num1 1
No Yes s
File running interface
Hotlid=off 2007-11-1 10:05:00
100
75
50
25
0
Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0
Time 1:00 02:00 01:45 00:00
File Name:Num1
User: Labnet Now Running •••
1c Temp=00.0 Time=00:00 Cycle 01 of 10
Total Time: 00h 56m Remaining Time:00h 56m
Stop Pause Start Skip View File

12
1.2 How to Set System Parameters
In the main screen press “System” to
enter the system parameter settings
menu. Press “Enter” to move the
cursor to the next item. This operation
is followed by the symbol “ ”. Press
“Up/Down” to scroll to an option, and
information can be entered by using
the keyboard. Press “Right/Left” to
move cursor’s position.
Press “Back” to go back to the
main menu.
Press “Save” to save parameters.
Note: When setting a parameter,
press the “Save” button, otherwise the changes will be lost.
1.2.1 “Date” and “Time” indicate the system time and date.
1.2.2 “Module Select” stands for module type, including two optional modes,
named “18*0.5” and “24*0.2”. The Module type will be preset for the type
of unit you purchased. If a different size block is later installed, this
setting will need to be changed.
1.2.3 “Control Mode” stands for default control model after power-on,
including two optional modes, named “Block” and “Tube”.
“Block” mode offers basic temperature control during the course of cooling &
heating only. Compared to the block, the cooling & heating rate of the samples
in the block are a little slower, therefore, the actual rate of change of the
sample temperature is different from the block temperature. The actual time for
the sample to reach the block temperature is longer than the time it takes for
the block to reach the set temperature. Therefore the dwell time of the sample
at the set temperature can be shorter than the dwell time of the block at the set
temperature.(Please refer to the following figure: relation between block
temperature and reagent temperature.)
System Parameter Setting
Interface
Block
Reagent
Hotlid=off 2009-02-28 10:06:00
Date(yy-mm-dd): 09-02-28
Time(hh-mm-ss): 10:06:19
Module Select: 24*0.2
Control Mode: Block
Hotlid Temp: OFF
Block Temp: 10C
Key Sound: OFF
Run End Sound: ON
Back Save 5

13
“Tube mode” controls the cooling & heating rate of the block, inconsideration
of the sample temperature changing at a slower rate. In tube mode, there is an
overshoot after reaching the set temperature, which allows the sample to reach
the set temperature faster, and will help the stay at the set temperature for a
longer period. (Please refer to the following figure: relation between block
temperature and reagent temperature.)
1.2.4 “Key Sound” and “Run End Sound” stand for an audible key tone when
pressing a key and an audible alert at the end of a run, respectively.
Block
Reagent

14
CHAPTER 4 TROUBLE SHOOTING
1 Failure Analysis and Processing Procedures
No.
Phenomenon
Failure Analysis
Troubleshooting
Disconnected power supply
Check power supply for correct
connection
Bad fuse
Replace it (250V 2.5A,Φ5×20mm
or 125V 5A, Φ5×20mm )
Switch failure
Replace switch
1
No display and the two beeps
missed when power on
Others
Contact Labnet or your distributor
Ventilation blocked
Clear the airflow blockage
Bad connection
Open the device, check the
connectors for reliable connection
2
Abrupt change in heating
speed or bad temperature
control accuracy
Cooling module failure
Contact Labnet or your distributor
Cooling module failure
3
Significant change in cooling
speed, or unavailable
temperature below ambient
Fan failed or stopped
Contact Labnet or your distributor
Bad sensor
4
Heating and cooling
terminated
Malfunction of all cooling
modules
Contact Labnet or your distributor
Bad contact between connectors
Loose connection between
connectors
Lid heater failure
5
Lid cannot be heated
Lid sensor failure
Contact Labnet or your distributor
Bad contact of chip with its
socket
6
Abnormal characters
displayed
Chip malfunctioning
Contact Labnet or your distributor
7
Inactive keys
Membrane panel damaged
Contact Labnet or your distributor

15
2 Error Messages
No.
Error Message
Possible Reasons
1
Error Message when running “Please contact the
distributor”，with “AD1213 failure”
The microchip can not communicate with the
AD1213. AD1213 broken or circuit board trouble.
2
Error Message when running “Please contact the
distributor.”，with “Heatsink temperature is
too high”
Heat sink temp. higher than 70℃，the
temperature sensor is broken, there is a short
circuit or the fan is not working.
3
Error Message when running “Please contact the
distributor.”，and "Heatsink sensor is
disconnected."
Heat sink temp. less than 5℃，the sensor broken,
there is a short circuit or the fan is not working.
4
Error Message when running "Please contact the
distributor."，and "Temperature sensor No.1 is
disconnected."
Block sensor No.1 reports an open circuit
5
Error Message when running "Please contact the
distributor."，and "Temperature sensor No.1 is
short-circuited."
Block sensor No.1 reports a short circuit
6
Error Message when running "Please contact the
distributor."，and "Hotlid sensor is
disconnected"
Hot-lid sensor reports an open circuit
7
Error Message when running "Please contact the
distributor."，and "Hotlid sensor is short-
circuited."
Hot-lid sensor reports a short circuit
Note:
During the warranty term, please contact Labnet or your distributor. If the instrument is opened
by an unauthorized individual the warranty is void.

16
APPENDIX 1
Wiring Diagram (1 of 2)
LCD1
1LCD
Heatersink
AC input_
220-240VAC
50/60Hz
A
C
N
Switch
F US E2
5* 20m m _ 2.5A
SW1
PE
F US E1
5* 20m m _ 2.5A
BLOCK
PCB2
BYQ904101000010_BLOCK
J1-1_TE+
J1-2_TE-
J1-3_SEN-SINK
J1-4_SEN-SINK
J1-5_SEN-BLOCK
J1-6_SEN-BLOCK
J1-7_AUXHEATER
J1-8_AUXHEATER
AC Line
Filter
NL
KEY1
1KEY-BOARD
HOTLID
PCB1
BYQ904102000110_HOTLID
LID-J1-1_SEN
LID-J1-2_SEN
LID-J1-3_HEATER+
LID-J1-4_HEATER+
LID-J1-5_HEATER+
LID-J1-6_HEATER-
LID-J1-7_HEATER-
LID-J1-8_HEATER-
LID-J2-1_SEN
LID-J2-2_SEN
LID-J2-3_HEATER+
LID-J2-4_HEATER-
CPU
PCB4
BYQ5041041000000_CPU
J13-1_HV+
J13-2_HV-
J13-3_VIN5
J13-4_HOTLID
J13-5_F AN
J13-6_PWM
J13-7_HEAT
J13-8_COOL J3_CPU_JTAG_IDC20
J10_KEY IN
J5_LCD_24P
J9_CPLD_JTAG_IDC10
J16-1_SEN-HOTLID
J16-2_SEN-HOTLID
J15-1_SEN-F AN
J15-2_SEN-F AN
J15-3_SEN-BLOCK
J15-4_SEN-BLOCK
VB1_SUB D9_RS232C
J17-1_H+
J17-2_H-
POWER
PCB3
BYQ5041042000000_POWER
EJ2-1_HV+
EJ2-2_HV-
EJ2-3_+5V
EJ2-4_HOTLID
EJ2-5_F A N
EJ2-6_PWM
EJ2-7_HEAT
EJ2-8_COOL
EJ4-1_F A N-
EJ4-2_F A N+
EJ3-1_HOTLID-
EJ1-2_TE-
EJ1-1_TE+
F J1-1_A CN
F J1_2_PE
F J1-3_A CL
EJ3-2_HOTLID+
F J1-4_P E
EJ3-3_LID-SEN1
EJ3-4_LID-SEN2
EJ5-1_H2+
EJ5-2_H2-
EJ6-1_AUX_HEATER
EJ6-2_AUX_HEATER
EJ7-1_LIDSEN
EJ7-2_LIDSEN
PE
A
C
L
U4
1
2
F AN+
F AN-
U5
123
45 6
L
I
N
P
E
N
I
N
NL P
E

17
Wiring Diagram (2 of 2)
N
POWER
PCB3
BYQ504104200000U
EJ2-1_HV+
EJ2-2_HV-
EJ2-3_+5V
EJ2-4_HOTLID
EJ2-5_F A N
EJ2-6_PWM
EJ2-7_HEAT
EJ2-8_COOL
EJ4-1_F A N-
EJ4-2_F A N+
EJ3-1_HOTLID-
EJ1-2_TE-
EJ1-1_TE+
F J1-1_A CN
F J1_2_PE
F J1-3_A CL
EJ3-2_HOTLID+
F J1-4_P E
EJ3-3_LID-SEN1
EJ3-4_LID-SEN2
EJ5-1_H2+
EJ5-2_H2-
EJ6-1_AUX_HEATER
EJ6-2_AUX_HEATER
EJ7-1_LIDSEN
EJ7-2_LIDSEN
PE
SW1
Filter
INLET
CPU
PCB4
BYQ5041041000000
J13-1_HV+
J13-2_HV-
J13-3_VIN5
J13-4_HOTLID
J13-5_F AN
J13-6_PWM
J13-7_HEAT
J13-8_COOL J3_CPU_JTAG_IDC20
J10_KEY IN
J5_LCD_24P
J9_CPLD_JTAG_IDC10
J16-1_SEN-HOTLID
J16-2_SEN-HOTLID
J15-1_SEN-F AN
J15-2_SEN-F AN
J15-3_SEN-BLOCK
J15-4_SEN-BLOCK
VB1_SUB D9_RS232C
J17-1_H+
J17-2_H-
L
U5
123
45 6
L
I
N
P
E
N
I
N
NL P
E
DC FAN
U4
1
2
F AN+
F AN-
Block
LCD1
1LCD
CPU Board
A
C
L
F US E2
5* 20m m _ 5A
Powerboard
PE
F US E1
5* 20m m _ 5A
HOTLID
PCB1
BYQ904102000110
LID-J1-1_SEN
LID-J1-2_SEN
LID-J1-3_HEATER+
LID-J1-4_HEATER+
LID-J1-5_HEATER+
LID-J1-6_HEATER-
LID-J1-7_HEATER-
LID-J1-8_HEATER-
LID-J2-1_SEN
LID-J2-2_SEN
LID-J2-3_HEATER+
LID-J2-4_HEATER-
Hotlid
Rated Voltage
input:
AC100-120V 50/60Hz
KEY1
1KEY-BOARD
Switch
A
C
N
BLOCK
PCB2
BYQ904101000010
J1-1_TE+
J1-2_TE-
J1-3_SEN-SINK
J1-4_SEN-SINK
J1-5_SEN-BLOCK
J1-6_SEN-BLOCK
J1-7_AUXHEATER
J1-8_AUXHEATER
Heatersink

18
APPENDIX 2 Parameter Values
Item
Parameter
Scale
Range
Note
1
Temp(ºC)
Temp. Control point
4.0~99.0
--:--
（set the value before
“：”through “Function”
key.）
The time of Temp. control is
infinite, meanwhile, the hotlid
turned off.
2
Time
Time of Temp.
Control（mm:ss）
00：00~59：59
0.1 ~5.0
3
Ramp(C/S)
Ramping Rate
（ºC/Second）
#.#
Maximum of Ramping Rate
4
+Temp
Temp. Gain of Every
Cycler
0.0~9.9
5
+Time
Time Gain of Every
Cycler（mm:ss）
0:00~9:59
Cycle1
Section Numbers
1~5
×00
Cycler Numbers from
beginning to the end
1~99
From 00
The start segment of
the section
X（and existed segment
no.）
6
to 00
The end segment of
the section.
≤X+15
Cycle1：×00 From 00 to 00
7
Grad.
Gradient Temp.
Difference（）
0 ~30
8
Date
Date(YY-MM-DD)
00（~99）-01（~12）-
01（~31）
year-month-date
9
Time
Time(HH:MM:SS)
00:00:00~23:59:59
hour：minute：second
10
Default File
The default file name
Max. length is 11
characters.
11
Default User
The default user
name
Max. length is 11
characters.
Block
Under this mode (once
reaching target temperature
namely entering into the
thermostable state), the time
for reagent’s temperature
under target temperature is
less than setting time (see Fig.
1).
12
Control Mode
Control Mode
Tube
Under this mode (once
entering into the thermostable
state after reaching target
temperature and decreasing
about 3°C), reagent’s
temperature can be close to
target temperature very fast
(see Fig. 2).
13
Sample Volume
Sample Volume（ul）
10~200
State of Hotlid: OFF
OFF
14
Hotlid Temp
State of Hotlid:
Open（）
30~99
15
Block Temp
State of Block Temp
during the heating of
Hotlid（ºC）
0~99
16
Key Beep
Key Sound (ON or
OFF)
OFF/ON
Humming
17
Run End Beep
Run End Sound (ON
or OFF)
OFF/ON
Humming continuously