Labnet MultiGene Mini User manual

MultiGeneMini
ThermalCycler
TC020-24 /TC020-24-230V
TC050-18 /TC050-18-230V
Operation Manual
Ver.1.2
Labnet International

CONTENTS
CHAPTER 1 SPECIFICATIONS ...................................................................................................... 1
1 Normal Operating Conditions .................................................................................................. 1
2 Transportation and Storage Conditions................................................................................... 1
3 Basic Specifications................................................................................................................. 1
4 Performance ............................................................................................................................ 2
5 Software Functions .................................................................................................................. 2
CHAPTER 2 Features ........................................................................................................................ 3
1 Description ............................................................................................................................... 3
2 Keyboard Diagram................................................................................................................... 4
3 Key Functions .......................................................................................................................... 4
4 Inspection before Power-up..................................................................................................... 5
5 Power-on Procedures .............................................................................................................. 5
CHAPTER 3 OPERATION GUIDE..................................................................................................... 6
1 How to Run a Program ............................................................................................................ 6
1.1 How to edit a program....................................................................................................... 7
1.2 How to Set System Parameters...................................................................................... 12
CHAPTER 4 TROUBLE SHOOTING............................................................................................... 14
1 Failure Analysis and Processing Procedures........................................................................ 14
2 Block Error Messages ........................................................................................................... 15
APPENDIX 1 Wiring Diagrams .................................................................................................... 16
APPENDIX 2 Parameter Values.................................................................................................. 18

1
CHAPTER 1 SPECIFICATIONS
This chapter describes the MultiGene Mini Thermal Cycler’s operation, transportation
and storage conditions, basic parameters, performance and functions.
1 Normal Operating Conditions
Ambient operating temperature: 10°C ∼30°C
Relative humidity: ≤70%
Power supply: ~100-120V, 200W, 50/60Hz
~220-240V, 200W, 50/60Hz
Note:
Before powering on, please confirm that the above operating
conditions are satisfied. The unit must be plugged into a
grounded outlet.
2 Transportation and Storage Conditions
Ambient temperature: -20 ºC～+55 ºC
Relative humidity: ≤80%
3 Basic Specifications
Model
Parameters
TC050-18
TC020-24
Sample Capacity
18
24
Tube Volume (ml)
0.5
0.2
Dimensions (mm)
297×212×200（L×W×H）
Display Screen
320×240 Backlit LCD
Weight (kg)
3.2
250V 2.5A (Ф5×20mm)
Fuse
125V 5A (Ф5×20mm)
Computer
Interface
RS232

2
4 Specifications
Temperature range: 4ºC～99ºC
Maximum Heating rate: ≥5.0ºC/sec(max)
Maximum Cooling rate: ≥4.0ºC/sec(max)
Temperature Resolution ± 0.1°C
Block Temperature accuracy: ≤±0.3ºC
Temperature display accuracy: ≤±0.5ºC
Block temperature uniformity: ≤±0.5ºC
Heated lid work temperature range: 105ºC±5ºC
Note: These performance specifications can be met when the unit is operated in Ambient
temperature: 23±5ºC,humidity≤70%
Temperature control accuracy and block temperature uniformity are tested at the following
temperature settings: 55ºC ， 72ºC ， 95ºC
Max. number of cycles: 99
Max. Segments within a cycle: 9
Max. temperature time: 99 min. 59 sec.
Max. incubation and in situ time: 99 hour 59 min.
Program storage capacity: 100
5Software Functions
File editing and saving
File accessing, modifying and deleting
Automatic file link
Heating/cooling rate setting
Automatic temperature and time increments/decrements during cycling
Real-time display of data at each phase of program execution
Pause
Stop
Auto-restart in case of power failure
Audible alarm
Run time estimation
Remaining time estimate
Date and time (year, month, day, hour, minute, second) display
Audible alarm in the event of failure
Note:
The above software functions are listed for reference only. Labnet
reserves the right to modify the software without notice.

3
CHAPTER 2 Features
This chapter covers the MultiGene Mini Thermal Cycler’s mechanics, the keyboard
and each key’s function, and preparations before power-on.
1 Description
①Heated lid & Block
②LCD Display
③Keyboard
④RS232 interface
⑤Power switch
⑥Power connecter
⑦Fuse Socket
Note:
1 The fuse specifications are as follows: 250V:2.5A / 125V:5A, Ø5×20mm. The fuse
should be replaced with one that meets these specifications.
2 Contact your distributor or Labnet’s service department if you need proper fuses.
4
3
2
1
7
6
5

4
2 Keyboard Diagram
3 Key Functions
“CE”，can be used to change “+, -” when editing
temperature and time.
“Right/Left”，moves the cursor one position to
the left or right during file parameter setting.
“Up/Down”，moving the cursor one position up or
down during file parameter setting.
“Enter”，press it to accept the present parameter
settings displayed on the screen.
“Function”, press any button to accept the
current items displayed above the buttons.
“Numbers & Letters”,
select the letter by pressing
the key until your selection
appears on the screen.

5
4 Inspection before Power-up
Before switching the power on, please ensure that:
1) Supply voltage matches the voltage rating label on the instrument. (refer to
Chapter 2);
2) The plug has been inserted into the power socket;
5 Power-on Procedures
Turn on the power (i.e. turn the power switch to position ‘1’), the MultiGene Mini
Thermal Cycler will beep 4 times, and the LCD will display ‘ ’. This
means the device is self-testing, which takes about 1-2 minutes. After the self-test is
complete, the main menu will be displayed, and the device is ready for the following
operations, editing, accessing, modifying, or deleting a file.

6
CHAPTER 3 OPERATION GUIDE
There are eight menu selections for the MultiGene Mini Thermal Cycler. In this
chapter, we will explain operation, for example, how to edit, access, modify, delete,
or run a protocol file and how to set parameters.
Warning!
If an abnormal sound or display appears on start up or if a failure error
during the Self Test mode, Turn off the power immediately and contact
Labnet or your distributor
Note:
If the number of samples is less than the number of wells in the block,
the sample tubes should be evenly distributed over the block, in order
to ensure that the heated lid will evenly press on the tops of the sample
tubes. This also ensures that both the block’s load and the temperature
distribution to each tube are even.
Note:
Before closing the cover, turn the knob counter-clockwise according to
the knob diagram until there is a "click....." Please note that the knob
can be tightened by turning it clockwise, After closing the cover but
before power on, turn the knob clockwise until you hear a click, then
turn the knob one more turn.
When the knob is turned clockwise or counter-clockwise, you will hear a
"click" sound, To open pull the release latch with your finger; and turn
the knob counter-clockwise.
1How to Run a Program
Switch on the power, the MultiGene Mini Thermal Cycler will beep 4 times. The
screen will display “ ”，and the instrument will carry on self test.
The self test takes about 1~2 minutes. If the self test is successful, the screen will
display the main Menu.
On the left top corner of the main
screen the Hot lid status is displayed.
The center of the screen displays
Labnet, the software version number,
block model, default file, default user,
control mode and sample volume.
Press “File” to enter file list
interface.
Press “System” to enter system
parameter setting menu.
Main Screen
Hotlid=020C 2009-04-01 10:05:00
Labnet
MultiGene Mini
Version: 2001-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
File System Run n

7
Hotlid=020C 2009-03-24 10:05:00
Labnet
MultiGene Mini
Version: 2009-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
Back Run n
If the default user does not have a password, press “Run” to run the default file. You
will be prompted to enter sample volume(if the unit is set for Tube Mode) in the pop
up window. If the unit is set for block mode, the unit will begin running the default file.
If the default user does have a password, you will be prompted to enter it before you
can run the default file.
Password Prompt
Use the keypad to enter the
password. Press “Back” to go back
the main menu. Press “Accept” to
confirm the password and enter file
running interface or sample volume
input window. If the password is
incorrect, a dialog box will be
displayed as below:
If the current Control Mode is Block
mode, press “Accept” button to
enter file running interface. If the
current Control Mode is Tube mode,
press “Accept” button, a dialog box
will be displayed to input the sample
volume.
Enter sample volume used. By using
Tube mode, the temperature
overshoot and time will be adjusted
for different sample volumes.
Press “Back” to go back the main menu.
Press “Run” to enter the file running menu.
1.1 How to Edit a Program
Press F1 “File” on the main screen to enter the file list menu. There are 3 files listed
as shown in the image. When the flashing bar is in the User Column, select users
with the up/down keys; The File Name Column will display the files of the selected
Hotlid=off 2009-04-01 10:05:00
Labnet
MultiGene Mini
Version: 2009-1.0
Default User: Labnet
Default File: 1
Module Select: 24*0.2
Control Mode: Block
Back Acceptt
Enter password
-
Please enter sample volume:
020µl
Please enter sample volume:
020µl
Password error. Try again.
-
Enter password
-

8
user. Shift to the File Name Column, using the “right” arrow button, then select the
user’s file using the up/down keys.
Press “Edit” to edit the file
indicated by the cursor.
Press “New File” to create a new
file.
Press “Delete” and you will be
prompted “Do you want to delete
the file? Please select ‘F3’or
‘F4’”, Select（Delete）or （Back）.
Press “Back” to go back to the
main menu.
If the file has no password, press
“Edit” to enter file editing interface;
If the file has a password, press
“Edit”, a dialog will be displayed to
indicate the user to enter the
password.
Enter the password. Press
“Accept” to confirm the password,
and the system will enter the file
editing menu; or press “Back” to go
back to the file list interface.
If the file has no password, press
“Run” to enter file run menu or enter
the sample volume. If the file is
password protected, press “Run”, a dialog box will be displayed to input the
password. Press “Back” to go back
the file list interface. Press “Accept”
to confirm the password.
After pressing “Run” in the file list
interface, and entering the
password (If Password protected):
If the current Control Mode is Block
mode, Press “Accept” to enter the
file running interface; If the current
Control Mode is Tube mode, press
“Accept”, and a dialog box will be
displayed to input sample volume.
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
AMP1
2007-07-28
2
Mike2
AMP2*
2007-07-28
3
Sue3
AMP3
2007-07-28
Back Accept t
Enter password
-
Please enter sample volume:
020µl
Enter password
-
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
Amp1
2008-07-28
2
Mike2
Amp2*
2008-07-28
3
Sue3
Amp3
2008-07-28
Edit New File Delete Back Run n
Hotlid=off 2008-07-28 10:05:00
No.
User
File Name
Save Time
1
Jeff1*
AMP1
2008-07-28
2
Mike2
AMP2*
2008-07-28
3
Sue3
AMP3
2008-07-28
Back Run n
Please enter sample volume:
020µl
File List Menu