Labnet Spectrafuge 6C User manual

Spectrafuge 6CSpectrafuge 6C
Spectrafuge 6CSpectrafuge 6C
Spectrafuge 6C
Compact Centrifuge
Labnet International
PO Box 841
Woodbridge, NJ 07095
Phone: [phone] Fax: [phone]
Email: [email protected]

Copyright May 2008, Labnet International.
All rights reserved.
Reproduction without prior written permission is prohibited.
Information contained herein is subject to change without notice.
Safety Precautions
NEVER use the centrifuge in any manner not specified in these instruc-
tions.
NEVER operate the centrifuge without a rotor properly attached to the
shaft.
NEVER fill tubes while they are in the rotor. Liquid spillage may harm
unit.
NEVER put hands in the rotor area unless the rotor is completely stopped.
NEVER move the centrifuge while the rotor is spinning.
NEVER use solvents or flammables near this or other electrical equipment.
NEVER centrifuge flammable, explosive or corrosive materials
NEVER centrifuge hazardous materials outside of a hood or proper
containment facility
ALWAYS load the rotor symmetrically. Each tube should be counterbal-
anced by another tube of the same type and weight
ALWAYS locate the centrifuge within easy access to an electrical outlet.
ALWAYS use only centrifuge tubes designed to withstand centrifugal
forces of at least 4,000 xg.
ALWAYS use a wrench to tighten rotor nut.
Notes

Table of Contents
1. General Information 1
Description
Safety precautions
Technical data
Accessories supplied with unit
Warranty
2. Installation 2
Unpacking the centrifuge
Required space
Installing the centrifuge
3. Installing the Rotor 3
Rotors and accessories
Rotor maintenance
Removing and installing the angle rotor
Overloadingrotors
4. Operation 6
Closing the lid
Lid release
Lid lock
Speed selection
Selection of operating time and momentary operation
Starting the Centrifuge
5. Service and Maintenance 8
Service
Cleaning
Disinfection
Replacing fuses
6. Troubleshooting 9
7. Where to Call 10
8. Determination of g-values 10
To use this chart, find the radius value on the radius scale. Place the
edge of a ruler on the value. Place the right side edge of the ruler on the
speed scale at the desired speed. The estimated RCF can then be read
from the RCF scale where the ruler edge passes through it. This chart
can also be used to determine the proper speed for the desired RCF
value. 11

1. General Information
This manual provides important safety information for the Spectrafuge 6C
laboratory centrifuge. It should be kept near the centrifuge for quick and
easy reference.
1.1 Description
The Spectrafuge 6C is a small benchtop centrifuge designed for
separation of various research samples. The Spectrafuge 6C is
supplied with an 6 x 15ml rotor. Adapters are available for tubes
smaller than 10ml. The Spectrafuge reaches speeds of up to
6,500rpm/4,000xg.
1.2 Safety precautions
Note: All users of the centrifuge must read the Safety
Precautions section of this manual before attempting to
operate the unit!
If this equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment
may be impaired.
Do not operate the centrifuge if any of the following conditions
exist:
-The centrifuge has not been installed properly
-The centrifuge is partially dismantled
-Service has been attempted by unauthorized or unqualified
personnel
-The rotor has not been installed securely on the motor shaft
-Rotors and accessories not belonging to the standard range are
being used without permission being obtained from the manu-
facturer to use such rotors and/or accessories in the centrifuge
Exception: Centrifuge tubes, normally available in the laboratory.
-The centrifuge is located in an explosive atmosphere
-Materials to be centrifuged are combustible and/or explosive
-Materials to be centrifuged are chemically reactive
-The rotor load is not properly balanced
!
1
Possible reason: Lid lock is jammed
Solution: Call for service
Possible reason: Centrifuge is not receiving power
Solution: See “Centrifuge will not start”
Centrifuge cannot be started, although power is on
Possible reason: Lid not closed correctly
Solution Close lid correctly
Possible reason: No speed or time has been selected
Solution: Set speed and/or time
Centrifuge displays error “03”
Possible reason: Lid opened prior to signal “00”.
Solution Close lid and open lid
7. Where to call
Should you have any questions about the Spectrafuge or its accesso-
ries, please call Labnet’s Customer Service Department at 732 417-
0700. Customer Service is staffed from 8:30am to 5:30pm, EST,
Monday through Friday. Our 24 hour fax number is 732 417-1750.
Inquiries may also be sent via our electronic mailbox at
[email protected].
Should your Spectrafuge require service, please call Labnet’s Techni-
cal Services Department at 732 417-0700. Our Service Department
is staffed from 8:30am to 5:00pm, EST, Monday through Friday. Our
24 hour fax number is 732 417-1750. Electronic mail may be sent to
[email protected].
Please have the unit’s serial number (located on the back panel of the
instrument) available when calling. Should an item require return to
Labnet for service, a repair return authorization (RRA) number must
first be received from Labnet. Items sent without an RRA number
will not be accepted.
8. Determination of g-values
The centrifuging radius of the 15ml rotor is 8.5cm for round bottom
tubes and 8.2 for conical bottom tubes. See Section 3.1 for the
correct radius when using adapters and smaller tubes. The chart on
the next page can be used to determine g-values.
10

1.3 Technical data
Dimensions
Width 8.25 inches
Depth 9.5 inches
Height 7.0 inches
Maximum speed 6,500rpm
Maximum RCF 4,000 x g
Maximumvolume 6 x 15ml
Admiss. density 1.2kg/dm3
Electrical/fuse rating 100V~,50-60Hz, 1.0A/1.6AT
120V~, 50-60Hz, 1.0A/2.5AT
230V~, 50-60Hz, 0.6A/1.25AT
1.4 Accessories supplied with centrifuge
Each unit is supplied with 1 instruction manual, 1 warranty card
and 1 power cord. Some models are supplied with a rotor screw
wrench.
1.5 Warranty
This centrifuge has been subject to thorough testing and quality
control. In the unlikely event of a manufacturing fault, our one
year warranty (from the date of delivery) covers the centrifuge
and the rotor. This warranty becomes invalid in the case of
incorrect operation, use of nonstandard spare parts or accesso-
ries and unauthorized modification of the rotor or centrifuge.
Labnet reserves the right to make technical modifications.
Statements contained herein are not to be considered binding.
2. Installation
2.1 Unpacking the centrifuge
Before unpacking the centrifuge, inspect the outside of the carton
for any shipping damage.
The centrifuge is delivered in a carton with protective cushions.
Remove the centrifuge from the carton. Retain the carton and
cushions until it has been established that the centrifuge is
workingproperly.
Inspect the centrifuge for any visible signs of shipping damage.
2
5.3 Cleaning the rotor
The rotor should be cleaned after each use. When spinning
samples containing phenol or phenol chloroform, the rotor should
be cleaned immediately after use.
5.4 Disinfection
Should a spill of infectious materials occur within the rotor or
chamber, the unit should be disinfected. This should be per-
formed by qualified personnel with proper protective equipment.
5.5 Replacing fuses
Check the fuse when it is recommended in the Troubleshooting
Guide located in this manual. The fuse holder is located in the
power inlet on the rear of the unit. Disconnect the power cord
from the power inlet. Open the fuse holder drawer by inserting a
small screwdriver under the tab and prying it open. Remove the
innermost (operative) fuse from its retaining tabs and replace the
fuse if necessary. A spare fuse is located in the outermost
chamber of the fuse drawer. Replace only with a fuse of exactly
the same value as the original. (Fuse type may be found in the
Technical data section of this manual.)
6. Troubleshooting Guide
Please refer to this guide before calling for service.
Centrifuge will not start
Possible reason: No power supply
Solution: Check that power is being supplied to
the outlet
Check that the power cord is plugged
into both the wall outlet and the back of
the centrifuge
Check that power cord is not damaged
Possible reason: Blown fuse
Solution: Check fuse and replace if necessary
Lid lock will not release
Possible reason: Defective lid lock
Solution: Open manually and have unit serviced
Possible reason: No power from PC board
Solution: Call for service
9

Shipping damage is the responsibility of the transportation carrier.
Any claims for damage must be filed within 48 hours.
The accessories supplied with the centrifuge should be kept with the
instruction manual near the centrifuge’s place of installation.
2.2 Required space
The centrifuge should be installed on a rigid, even surface such as a
stable laboratory bench, cabinet, etc. To guarantee sufficient ventila-
tion, ensure that the centrifuge has at least 15cm (6 inches) of free
space on all sides, including the rear.
The centrifuge should not be located in areas subject to excessive
heat such as in direct sunlight or near radiators or the exhaust of a
compressor, as a buildup of heat may occur within the chamber.
2.3 Installation
Before operating the centrifuge, check that the power source corre-
sponds to that on the manufacturer’s rating label, then connect the
power cord to the centrifuge and the power source.
3. Installationorrotorsandrotormaintenance
3.1 Rotors and accessories
The following accessories are available for the Spectrafuge:
Angle rotor for 6 x 15ml tubes
Order no. Included with unit (C0060-RTR)
Tube measurement 15ml(17x125mm)-10ml(16x100mm)
Max. speed 6,500rpm
Centrifugingradius 8.5cm
RCF (g-value) 4,000 x g
Adapter for 5ml(12x75mm) and 7ml (13x100mm) tubes
Order no. C0200-17A
Tube measurement 12x75mm,13x100mm,orcommom
Sarstetd style tubes
3
4.5 Selection of operating time and momentary operation
Time can be selected in half minute intervals from 0.5 to 10
minutes, and in one minute intervals from 11 to 30 mintes. Time
can also be set to continouos/hold by turning the timer knob past
the thirty minute position. This will display the continouos setting
“On”.
When the preselected time expires, the centrifuge will stop
automatically. To stop the centrifuge prior to the expiration of set
time, press the “start/stop” knob.
The centrifuge may be operated manually by pressing and holding
the “start/stop” knob. The centrifuge will continue to run as long
as the knob is depressed.
4.6 Starting the centrifuge
Once the time and speed have been set the centrifuge can be
started by pressing the “start/stop” knob. The centrifuge will the
run for the specified amount of time.
5. Service and Maintenance
5.1 Centrifuge service
The Spectrafuge 6C requires no routine maintenance other than
the occasional routine cleaning. All repairs should be performed
by authorized, qualified personnel only. Repairs performed by
unauthorized personnel may void the warranty.
5.2 Cleaning the centrifuge
Always keep the centrifuge housing, rotor chamber, rotor and
rotor accessories clean. All parts should be wiped down periodi-
cally with a soft cloth. For more thorough cleaning, use a neutral
cleaning agent (pH between 6 and 8) applied with a soft cloth.
Excessive amounts of liquid should be avoided. Liquid should not
come into contact with the motor. After cleaning, ensure that all
parts are dried thoroughly by hand or in a warm air cabinet
(maximum temperature 50ºC)
8

4
4.1 Closing the lid
After the rotor has been properly secured and loaded, close the
centrifuge lid, making sure that the interlock has been engaged.
4.2 Lid release
Following a run, the centrifuge will display will show flashing
“00”. This signals the end of a run and the lid can now be
opened by pressing the “lid knob” (left). Note that the lid can not
be opened until the display flashes “00” and the rotor has
stopped.
WARNING: Do not attempt to open the lid of any centri-
fuge until the rotor has come to a complete stop.
In the event of a power failure or malfunction, it may be neces-
sary to open the lid manually.
1. Disconnect the power cord from the wall socket.
2. Remove the plastic plug, located on the left side of the unit,
below the quick button.
3. Pull the cord (attached to the plug) to open the lid lock
manually.
4.3 Lid lock
The centrifuge can be started only with the lid securely closed.
Do not attempt to open the lid until the end of run signal “00” is
displayed.
4.4 Speed selection
The speed (rpm) can be selected from 300 to 6,500rpm with the
knob (right). The set speed can be viewed at all times on the
large LED display (right).
Figure4.
Spectrafuge
controlpanel
layout
7
Tube and tube adapter reference chart.
3.2 Rotor maintenance
The rotor should be cleaned thoroughly after each use. Thor-
ough cleaning must be performed when spinning samples
containing phenol or phenol chloroform. Periodically inspect
the rotor for dents, dings, scratches, discoloration and cracks. If
any damage to the rotor is found, discontinue use of the rotor
immediately and replace.
3.3 Removing and Installing the angle rotor
Remove the rotor screw from the motor shaft by turning the
screw counterclockwise. Lift the rotor upward and remove from
the centrifuge. Ensure that the motor shaft adapter remains on
the motor shaft (Figure 1). Clean the motor shaft and motor
shaft adapter (see figure 1).
Place the rotor on the motor shaft (figure 1) and over the motor
shaft adapter (see figure 1 and 2). Note: Figure 1 and 2 are
located on the following page.
When loading the rotor, refer to figure 3 (located on the following
page). Loading in the pattern indicated will ensure a balanced
load. Tubes to be loaded should be filled equally by eye. The
difference in the weight between the tubes should not exceed 2-3
grams. Apartially loaded rotor may be centrifuged if the loading
scheme for balancing a rotor given in figure 3 is followed.

Figure 3. Loading the rotor
Figure 2. Bottom of angle rotor
5
3.5 Overloading the rotor
The maximum load of the rotor and the maximum speed have
been established by the manufacturer. Do not attempt to
exceed these values.
The maximum speed of the rotor has been measured for liquids
having a homogeneous density of 1.2g/ml or less. In order to
centrifuge liquids with a higher density it is necessary to reduce
the speed.
Failure to reduce the speed may result in damage to the
rotor and centrifuge.
The revised maximum speed can be calculated with the following
formula:
Reduced speed (nred) = x max speed (nmax)
Example:
Where the density of the liquid is 1.7, the new maximum speed
would be calculated as follows:
nred = x 6,500 = 5,461 rpm
If in doubt concerning maximum speeds, please contact the
manufacturer for assistance.
4. Operation
ATTENTION: Never attempt to operate the centrifuge with
rotors or adapters that show signs of corrosion or mechanical
damage. Never centrifuge strongly corrosive materials that
may damage the rotors or accessories.
1.2
higher density value
1.2
1.7
6
Figure 1. Chamber and motor shaft