Leica DM3 XL User manual

Leica Microsystems CMS GmbH. Instructions for use, 11934142, Revision 1.0 from 2016-11-15
From Eye to Insight
Leica DM3 XL
Instructions for use •Gebrauchsanweisung •Mode d’emploi

Leica Microsystems CMS GmbH. Instructions for use, 11934142, Revision 1.0 from 2016-11-15
From Eye to Insight
Leica DM3 XL
Instructions for use

2
Copyrights
copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduc-
tion of text or illustrations (in whole or in part)
by print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following docu-
mentation reflect state-of-the-art technology. We
have compiled the texts and illustrations as ac-
curately as possible. Still, we are always grateful
for comments and suggestions regarding poten-
tial mistakes within this documentation.
The information in this manual is subject to modi-
fication at any time and without notification.
Revision 1.0, published 2016-11-15 by:
Leica Microsystems CMS GmbH
Ernst Leitz-Straße 17-37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS

3
Contents
contents
1. Important Notes about this Manual.............4
1.1 Text Symbols, Pictograms and their
Meanings..........................................................4
2. Intended Purpose of the Microscope ..........6
3. Safety Notes.....................................................8
3.1 General Safety Notes .....................................8
3.2 Electrical Safety ..............................................9
3.3 Transport and Storage..................................10
3.4 Notes on Handling Light Sources...............11
3.5 Notes on Handling Immersion Oil...............11
3.6 Notes on Handling Acids and Bases .........11
3.7 Disposal .........................................................11
4. Overview of the Instrument.........................12
5. Unpacking the Microscope ........................16
6. Assembling the Microscope.......................18
6.1 Stage ...............................................................18
6.2 Tube and Eyepieces......................................19
6.3 Objectives.......................................................19
6.4 Illumination.....................................................20
6.4.1 LED Lamp Housing LH113.............................20
6.5 Equipping the Incident Light Turret Disk ...21
6.6 Analyzer..........................................................22
6.7 Optional Accessories ...................................23
7. Startup.............................................................25
7.1 Switching On..................................................25
7.2 Köhler Illumination........................................25
7.2.1 Incident Light .................................................25
8. Operation........................................................26
8.1 Switching On..................................................26
8.2 Focusing..........................................................26
8.3 Tubes ...............................................................27
8.4 Eyepieces .......................................................28
8.5 Objectives.......................................................29
8.6 Light Sources.................................................30
8.7 Aperture Diaphragm.....................................31
9. Contrast Methods..........................................32
9.1 Incident Light .................................................32
9.1.1 Bright Field (RL) .............................................32
9.1.2 Oblique Illumination (RL)..............................32
9.1.3 Polarization (RL).............................................33
9.1.4 Interference Contrast ICR............................33
9.1.5 Dark Field (RL)................................................34
9.1.6 Fluorescence .................................................34
10. Measurements with the Microscope........35
10.1 Linear Measurements ..................................35
10.2 Thickness Measurements ...........................36
11. Trouble Shooting...........................................37
12. Care of the Microscope ..............................40
12.1 Dust Cover......................................................40
12.2 Cleaning..........................................................40
12.3 Handling Acids and Bases...........................41
12.4 Changing Fuses .............................................41
13. Essential wear and spare parts .................42
14. Index ...............................................................43
15. EU Declaration of Conformity.....................44

4
1. Important Notes about this Manual
1.1 Text Symbols, Pictograms and their Mean-
ings
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be
read carefully before the microscope is as-
sembled, put into operation or used. It must
therefore be kept safely for future reference.
1. iMportant notes about this ManuaL
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
its accessories.
(1.2)
→p.20
!
WARNING
CAUTION
Numbers in parentheses such as “(1.2)”, corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with an arrow, e.g. →p. 20, refer to a
specific page of the manual.
WARNING indicates a hazard with a medium
degree of risk that, if not avoided, can result in
death or serious injury.
CAUTION indicates a hazard with a low degree
of risk that, if not avoided, can result in minor or
moderate injury.
Caution!
Special safety instructions within this manual
are indicated with the triangle symbol shown
here, and have a gray background.
Notice! The macroscope and accessories can be
damaged when operated incorrectly.

5
1. Important Notes about this Manual
MM/YYYY
Warning of hazardous electrical voltage! Risk of
electrical shock!
Danger due to hot surface.
Warning of optical radiation! Never look directly
into the light beam! Wear safety goggles!
Warning of electromagnetic field
Warning of UV radiation! Wear safety goggles!
Warning of hazardous laser radiation – take ap-
propriate safety precautions!
Instructions for disposing of the instrument,
its accessories and consumables.
Connection to ground!
Explanatory note.
Item not contained in all configurations.
Manufacturer’s address with manufacturing date
China RoHS 50 years EFUP
(Environmentally friendly use period)
*

6
2. Intended Purpose of the Microscope
2. intenDeD purpose of the Microscope
The Leica DM3 XL microscope, to which this user
manual belongs, is designed for industrial, min-
eralogical and geological routine and research
applications.
Simultaneously, the requirements in EU Direc-
tive 2014/35/EU regarding the safety of electrical
equipment, and 2014/30/EU regarding electro-
magnetic compatibility have been met for all the
devices mentioned above.
According to the VO1194/2012 regulation regard-
ing the implementation of the Ecodesign Direc-
tive 2009/125/EC, microscopes are considered
special products. The light sources in this instru-
ment are special lamps which are only suitable
for microscopes. They are not suitable for gen-
eral lighting purposes or room lighting.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or not
using them within the specifications of Leica
Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
WARNING
Notes on handling laser devices
In the standard design without additional la-
ser safety precautions, the microscopes are
not suitable for coupling laser radiation (such
as to the camera ports), since this radiation
poses a hazard for the user (eye injury in par-
ticular).
For use of the microscope with lasers, Leica
Microsystems offers special microscope
variants with additional safety features. La-
ser couplings require corresponding safety
devices that have to be inspected and in-
stalled by trained personnel.
For further information, please contact your
authorized Leica Microsystems representa-
tive.

7
2. Intended Purpose of the Microscope
Reasonably foreseeable misuse
The following are prohibited:
• Using the microscope for any purpose not in
accordance with the Declaration of Conformity
(e.g. using it as an in-vitro diagnostic product
according to Council Directive 98/79/EC or as
a medical product according to Council Direc-
tive 93/42/EEC)
• Making use of auxiliary equipment to use the
area between the microscope stage and ob-
jectives as a clamp or holder (working like a
vice).
• Operating the microscope in an inclined posi-
tion.
• Cleaning the microscope in a way other than
specified in the manual.
• Allowing unauthorized personnel to open the
instrument.
• Using cables that Leica has not provided or
permitted.
• Using combinations with non-Leica compo-
nents that go beyond the scope of the manual
• Using power supplies that are not approved by
Leica.
• Operating light sources if they are not con-
nected.

8
3. Safety Notes
3. safety notes
3.1 General Safety Notes
These devices of protection class 1 are built and
inspected in accordance with EN 61010-1 / IEC
61010-1 Safety requirements for electrical equip-
ment for measurement, control and laboratory
use.
They also meet the requirements in EN 61326-1
/ IEC 61326-1 Electrical equipment for measure-
ment, control and laboratory use – EMC require-
ments.
It also complies with EN 62471 / IEC 62471, Photo-
biological safety of lamps and lamp systems, and
belongs to the exempt level (no photobiological
risk).
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The instruments and accessories described
in this manual have been safety-tested and
checked for possible hazards.
Before modifying the instrument in any way
or combining it with non-Leica products not
dealt with in this manual, it is essential to
consult the Leica agency for your area or the
main factory in Wetzlar!
Any unauthorized interference with the in-
strument or use of the instrument for appli-
cations for which it is not designed will au-
tomatically cancel any warranty claim and
product liability!

9
3. Safety Notes
3.2 Electrical Safety
General specifications
For indoor use only.
Supply voltage: 100-240 V AC
Frequency: 50/60 Hz
Power input: max. 40 VA
LED: max. 15 W
Fuse: 5x20, T 0.63A H, 250 VAC
Ambient temperature: 15-35°C
Relative humidity: max. 80% up to 30°C
(non-condensing)
Overvoltage category: II
Pollution degree: 2
WARNING
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
Caution!
Through activating to the grounding connec-
tion (earth screw on the back of the micro-
scope) ancillary equipment with its own and/
or extra power supply may be brought to the
same ground wire potential. For connections
without a ground connector, Leica Service
must be consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Bridging the fuse holder is
not permitted. The use of incorrect fuses may
result in a fire hazard.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water. Wa-
ter can cause electric shock.
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
WARNING
Before exchanging the fuses or lamps, be
absolutely certain to switch-off the main
power switch and remove the power cable.
WARNING
Only use original power cord, or alternate
VDE- / HAR-certified power cords which
meet the minimum requirements of 3x0,75mm2
and 10A/250V.

10
3. Safety Notes
WARNING
Per definition, the connection between
power supply cable and appliance inlet is
the power separator of this device. Thus, the
user is responsible for sufficient clearance
and accessibility of this area at all times.
Caution!
Do not use the microscope in altitudes ex-
ceeding 2000 m ASL/NL.
Caution!
Do not use this instrument near sources of
high electromagnetic radiation (for example,
unshielded, intentionally operated ultra-high
frequency sources), because these can dis-
rupt proper operation.
We recommend assessing the electromag-
netic environment before operation of this
instrument and then giving corresponding
instructions.
This device has been designed and tested
in accordance with CISPR 11 Class A. It may
cause radio interference in domestic envi-
ronment. In this case you may have to take
measures for removal of disturbance.
3.3 Transport and Storage
Caution!
Fully equipped, the microscope weighs more
than 18 kg. For transportation, the user has to
take care of the corresponding actions.
See also p. 17.
Caution!
Transport and storage in a range of -20°C –
+85°C and at a humidity not exceeding 90%
(non-condensing).

11
3. Safety Notes
3.4 Notes on Handling Light Sources
CAUTION
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps have to be operated in closed housings
and only after being mounted.
Never look directly into the beam path (blind-
ing hazard).
CAUTION
Lamps and lamp housings may be hot!
They must be placed at least 10 cm away
from the wall and away from flammable sub-
stances.
Particulary, supply and data lines must not
encounter lamp housings!
3.5 Notes on Handling Immersion Oil
Caution!
When using immersion oil, take care to avoid
skin contact! Ask the supplier for a safety
data sheet!
3.6 Notes on Handling Acids and Bases
For examinations using acids or other aggressive
chemicals, particular caution must be taken.
Caution!
Be absolutely certain to avoid direct contact
with these chemicals.
3.7 Disposal
After the end of the product’s life, please contact
Leica Service or Leica Sales on how to dispose
of it.
Please observe the national laws and ordinances
which, for example, implement and ensure com-
pliance with EU directive WEEE.
Note!
Like all electronic instruments, the micro-
scope, its components and expend-ables
may not be disposed of as general household
waste!

12
4. Overview of the Instrument
4. overview of the instruMent
Specification Leica DM3 XL
Contrast Method • IncidentLight: Brighteld,Darkeld,Polarization,
Differential Interference Contrast,
Fluorescence, Oblique Illumination
Incident Light Axis
with 4x Filter Turret Disc
Incident-light illuminator for up to eyepiece field number 22
with
• 4xfilter turret disc (BF, DF, POL, IRC, FLUO, OBL)
• 2lterpositions,selectable
• Color-codedcenterableaperture
• Pre-centeredelddiaphragm
• Manualbrightnessadjustment
Illumination • LEDlamphousingLH113
Tube Optionally with
• Fixedorvariableviewingangle
• Upto3switchingpositions
• Oneortwocameraports
• Ergotubewithheight-adjustableeyelevelandcamera
port
• Withuprightimage
Magnification Changer
(optional)
• Manual
• Magnicationsteps:1x;1.5x;2x
Objective Turret • Manual
• 5xforobjectiveswithM32thread
• Objectiveprismslide
XY Stage • Coaxialpinion
Focusing • Focusdialforcoarseandneadjustment
• SpeedSwitch(optional)
• Adjustablefocusthresholdandmovementrate

13
4. Overview of the Instrument
Fig. 1 Left side of the microscope Leica DM3 XL
1 LED lamp housing LH113
2 Coarse and fine focusing
3 Intensity adjustment
4 Coaxial pinion for x/y stage movement
5 Specimen stage with specimen holder
6 Objective turret with objectives
7 Objective prism slide
8 Tube
9 Eyepiece tube
10 Eyepieces
11 Analyser
12 Centering keys for aperture diaphragm
13 Aperture diaphragm centering
2
3
5
6
12
11 10
9
8
7
4
1
13

14
4. Overview of the Instrument
Fig. 2 Right side of the microscope Leica DM3 XL
1 Eyepieces
2 Eyepiece tube
3 Tube
4 Objective prism slide
5 Objective turret with objectives
6 Specimen stage with specimen holder
7 On/off switch
8 Coarse and fine focusing
9 Coaxial pinion for x/y stage movement
10 Incident-light axis
11 Color-coded aperture diaphragm and adjustment of
oblique illumination
12 Filters (2)
13 Filter turret
14 Polarizer
15 LED lamp housing LH113
1
2
3
4
5
6
789
10
11
12
13
14
15

15
4. Overview of the Instrument
Dimensions (specification in mm)
424
496
354
405
115

16
5. Unpacking the Microscope
5. unpacking the Microscope
Allowable ambient conditions:
Temperature 15–35°C
Relative humidity: max. 80% up to 30°C
non-condensing
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
fungus contamination.
See the chapter on „Care of the Microscope“ →
p. 40 for additional instructions.
Caution!
Electrical components must be assembled
at least 10 cm from the wall and away from
flammable substances.
Caution!
When installing the microscope, make sure
the power inlet is freely accessible so that
the instrument can be quickly disconnected
from the mains if necessary.
First, carefully remove all components from the
transportation and packaging materials.
Note:
If at all possible, avoid touching the lens surfaces
of the objectives. If fingerprints do appear on the
glass surfaces, remove them with a soft leather
or linen cloth. Even small traces of finger perspi-
ration can damage the surfaces in a short time.
See the chapter on „Care of the Microscope“ →
p. 40 for additional instructions.
Caution!
Do not connect the microscope and periph-
erals to the power supply at this point under
any circumstances!
Installation location
Work with the microscope should be performed
in a dust-free room, which is free of oil vapors
and other chemical vapors, as well as extreme
humidity. At the workstation, large temperature
fluctuations, direct sunlight and vibrations should
be avoided. These conditions can distort mea-
surements and micrographic images.

17
5. Unpacking the Microscope
Transport
For shipping or transporting the microscope and
its accessory components, the original packag-
ing should be used.
As a precaution to prevent damage from vibra-
tions, the following components should be disas-
sembled and packaged separately:
• Unscrewtheobjectives.
• Removetheincidentlightaxis.
• Removethecoaxialpinion.
• Removethelamphousings.
• Removeallmovingorlooseparts.
Weight
The weight of the microscope depends on the
particular equipment.
Fully equipped, the microscope weighs more
than 18 kg. For transportation, the user has to
take care of the corresponding actions.
Caution!
For transporting it is essential to remove all
components listed under “Transport”!

18
6. Assembling
Fig. 3 Specimen stage with specimen holder
6. asseMbLing the Microscope
The microscope components are logically as-
sembled in this order:
• Stagewithaccessories
• Incidentlightaxis
• Intermediatesystems*
• Tube
• Eyepieces
• Objectives
• LEDlamphousingLH113
• Polarization*
Only one commonly used screwdriver is neces-
sary for assembly, which is included in the deliv-
ery package.
The tool can be stored on a magnetic retainer on
the underside of the stage at the right.
When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter “Optional Accesso-
ries” →p. 23.
6.1 Stage
!Caution:
Before completing the stage, make sure no ob-
jectives are installed!
• Place the stage and fasten it with the four
screws
Remove the screw located under the stage in the
front.
Specimen Holder
• Placethespecimenholderonthestage.