Optika B-1000 POL User manual

Version: 1
Issued: 01, 12, 2015
Model
B-1000MET
B-1000 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm
M-613 Polarizing set (lters only)
M-615 Lambda lter for polarizing set
M-617.1N Phase contrast set with IOS PLAN objective 40x
M-781 WF10x/22mm micrometer eyepiece (10mm, 0.1mm div.)
CL-35 Halogen Lamp 24V 100W (only for metallurgical attachment M-1035)
M-690 Eyecup (pair)
M-619 Photo adapter for REFLEX camera with FULL FRAME sensor
M-173 Photo adapter for APS-C and Full Frame Reex cameras
M-699 Universal adapter for M-114, M-116, M-173 and eyepiece cameras
M-620 C-Mount adapter for 1/3” sensor.
M-620.1 C-Mount adapter for 1/2” sensor.
M-114 C-Mount adapter for 1/2” sensor.
M-116 C-Mount adapter for 2/3” sensor.

Page 5
Overview
STAGE LOCK
KNOB
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
PHOTO PORT
EYEPIECE
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
FIELD DIAPHRAGM
FINE FOCUSING
KNOB
EXTERNAL
POWER
SUPPLY
OBJECTIVE
STAGE
INCIDENT
ILLUMINATION
ATTACHMENT

Page 6
Put the microscope stand on a
solid table.
First insert the incident illu-
mination attachment, using
the 2,5mm Allen wrench to
tighten the screw. All screws
are already inserted into each
threaded hole.
(Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
①Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Polarizer/Analyzer
⑥ Stage
⑦ Objectives
⑧ Extension Tube
⑨ Lamp house
⑩ External power supply
⑪ Incident illumination attachment
①
②
③
④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
⑪
Fig.1

Page 7
Insert the condenser under
the stage: push until it is well
inserted into its holder (under
the condenser there is a pin
that must fully enter the guide
of the holder). (Fig.6)
Insert the lamp house into the
extension tube, then use an
allen wrench to x it. (Fig. 4)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Insert the optical head above
the attachment using the other
3mm Allen wrench to tighten
the screw. (Fig.2)
Insert both eyepieces into
the tubes of the optical head.
(Fig.5)
Insert the extension tube
on the rear of the incident
illumination attachment, then
use an allen wrench to x it.
(Fig.3)
Fig.2
Fig.5
Fig.3
Fig.6
Fig.4

Page 8
Insert the power supply cable
for incident light. (Fig.10)
Connect the cable to the
external power supply. (Fig.9)
Insert the power supply jack
on the connector at the rear.
(Fig.11)
Fig.9
Fig.10
Fig.11
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.8)
Fig.8
Mount the stage: lower the
support using the coarse
focus knob, then place the
stage and rmly tighten the
lock screw. (Fig.7)
Fig.7

Page 9
B-1000 illumination can be managed through
the keyboard placed on the left of the stand:
1) ON-OFF: press this button to turn on or off
the LED illuminator.
2) BOOST: press this button in order to incre-
ase the brightness (useful for high-magnica-
tion objectives or very opaque specimens).
2) AUTO-OFF: if you want the illuminator to
switch off automatically, press this button until
15, 30 or 60 minutes delay is set. After this
period of time, the light will turn off. You have
to press the ON-OFF button to turn it on again.
(Fig.12)
In order to activate the illuminator, insert the
plug of the external power supply into the
socket and turn on the main switch on the side
of the main body. Press the ON-OFF button on
the control keyboard and turn the brightness
adjustment knob to a brightness suitable for
observation. (Fig.13)
Do not enable boost mode while observing with
low magnication objectives (4x, 10x) with fully
open diaphgram: the high brightness may hurt
user’s eyes.
Control keyboard
LED settings
Preliminary settings
1) ON-OFF
3) AUTO-OFF
2) BOOST
MAIN
SWITCH
• The eld diaphragm is
fully open. (Fig.14)
• The aperture diaphragm
is fully open. (Fig.15)
• No lter is inserted under
the observation head.
Using the microscope
Fig.12
Fig.13
Fig.14
Fig.15
Before trying to focus the
sample, ensure that:

Page 10
Loosen the lock-screw, turn
the observation head to
a comfortable position for
observation, and then lock the
lock-screw. (Fig.18)
Hold the right and left parts of
the observation head using
both hands and adjust the
interpupillary distance by
turning the two parts until one
circle of light can be seen.
(Fig.19)
Adjust the observation
head
Adjust interpupillary
distance
• The selector on metal-
lurgical attachment is in
“BF” (Brighteld position)
(Fig.16)
• Photo port closed
(lever on observation
head fully in).
• (If you use 5X objective)
Swing out lens of the
condeser in OUT position.
(Fig.17)
Fig.16
Fig.17
Fig.18
Fig.19