Leica M165 FC User manual

Leica M165 FC
Leica M205 FA / M205 FCA
User Manual

Leica M Series User Manual 2
General Instructions
Safety concept
Before using your microscope for the first
time, please read the "Safety concept" book-
let included with your instrument. It contains
additional information about handling and
care.
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
ODo not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
ONever use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
OIn most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accesso-
ries for use in clean rooms.
Servicing
ORepairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
OEnsure that the Leica stereo microscope
is operated, maintained and repaired by
authorized and trained personnel only.

Leica M Series User Manual 3
Important Safety Notes
User manual
The individual modules of the Leica M stereo
microscopy series include an interactive
CD-ROM with all relevant user manuals in
20 other languages. Keep it in a safe place,
and readily accessible to the user. User manu-
als and updates are also available for you
to download and print from our website at
www.stereomicroscopy.com.
This User Manual describes the special func-
tions of the individual modules of the Leica M
stereo microscopy series and contains impor-
tant instructions for their operational safety,
maintenance, and accessories.
The "Safety concept" booklet contains addi-
tional safety information regarding the service
work, requirements and the handling of the
stereo microscope, accessories and electrical
accessories as well as general safety instruc-
tions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above.
In particular, please observe all safety instruc-
tions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Special manuals
Special manuals are provided for a number of
accessories:
OUser Manual M2-216-1 for lamp housing
with high-pressure mercury burner.
OUser Manual for ebq 100 isolated supply
unit for 100 W high-pressure mercury
burners.
OUser Manual M2-267-1 for the motorized
focus system.
OUser Manual M2-166-2 for FluoCombi III.

Leica M Series User Manual 4
Symbols Used
Warning of a danger
•This symbol indicates especially impor-
tant information that must be read and
complied with.
Failure to comply can cause the following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Danger due to hot surface
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
OThis symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.

Leica M Series User Manual 5
Safety Instructions
Description
The individual modules fulfill the highest
requirements for observation and documenta-
tion of Leica stereo microscopes of the Mseries.
Intended use
ORefer to "Safety Concept" booklet
Non-intended use
ORefer to "Safety Concept" booklet
Never use M series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
Place of use
ORefer to "Safety Concept" booklet
OElectrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
OAvoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
OIn warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
ORefer to "Safety Concept" booklet
Ensure that:
OThe M series stereo microscopes and
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
OAll operators have read, understood and
observe this User Manual, and particularly
the safety instructions.

Leica M Series User Manual 6
Safety Instructions (Continued)
Repairs, service work
ORefer to "Safety Concept" booklet
OOnly original Leica Microsystems spare
parts may be used.
OBefore opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause
injury.
Transport
OUse the original packaging for shipping or
transporting the individual modules of the
Leica M stereo microscopy series and the
accessory components.
OIn order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
ORefer to "Safety Concept" booklet
Disposal
ORefer to "Safety Concept" booklet
Legal requirements
ORefer to "Safety Concept" booklet
EC Declaration of Conformity
ORefer to "Safety Concept" booklet
Health risks
•Workplaces with stereo microscopes
facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
OOptimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
OThorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects.
OThe ergonomic design and construction of
the Leica M stereo microscopy series are
intended to reduce the exertion of the user
to a minimum.

Leica M Series User Manual 7
Safety Instructions (Continued)
•Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Light sources: Safety regulations
Protective measures of the manufacturer:
OUV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
ODummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
OUV filters are installed in the observation
beam paths to protect the eyes.
OThe stray-light protection on the lamp
housing prevents irradiation of the hands.
Warning
•UV radiation could damage the eyes.
Therefore:
ONever look into the light spot on the speci-
men plane without a UV protection screen.
ONever look into the eyepieces if no excita-
tion filter is in the beam path.
OFill empty filter positions with dummy filter
carriers (e.g. M165 FC).
ODo not select a white, strongly reflective
background for the specimen.

Leica M Series User Manual 8
Safety Instructions (Continued)
Supply unit
Always unplug the supply unit from the power
supply:
OWhen installing and disassembling the
lamp housing
OBefore opening the lamp housing
OWhen replacing the high-pressure mercury
lamp and other parts, such as the heat-
absorbing filter or the collector
ODuring maintenance work on the supply
unit
Lamp housing
•Never open the lamp housing while the
lamp is switched on. Risk of explosion,
UV exposure, blinding!
•Before opening the lamp housing, allow
it to cool off for at least 15 minutes.
Danger of explosion!
•Never cover the air duct on the lamp
housing. Danger of fire!
Mercury lamp
OFollow the user manual and safety instruc-
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
OFor transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
OWhen it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).
OTo minimize the risk of explosion, replace a
discolored mercury lamp promptly.
OLeica Microsystems assumes no liability
for damage caused by exploding, incor-
rectly installed or improperly used mercury
lamps.

Leica M Series User Manual 9
Contents
General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Instructions 5
The Leica M Series for Fluorescence
Congratulations! 13
A Step Towards Infinity 14
The Electronics: Comfort, Convenience and Safety for Your Experiments15
The Modular Design: Everything is Relative 16
Maximum Compatibility 17
On We Go 18
Assembly
Assembling the Focusing Column for TL Bases 20
Assembling the Focusing Column With an Incident-light Base 21
Motorized Focus: Restricting the Travel Path 22
Optics Carrier 23
Tube 24
Eyepieces 25
Objective 26
UV Protection Screen: Assembly 27
Objective Nosepiece – Assembly 28
Objective Nosepiece – Adjusting Parfocality 29
Leica FluoCombi III – Preparations 30
Leica FluoCombi III – Assembly 31
Leica FluoCombi III – Adjusting the Parfocality 33
Leica FluoCombi III – Centering the Objectives 35
Leica FluoCombi III – Filter and UV Protection Screen 36
Transmitted-light Base Leica TL BFDF: Before First Use 37
Transmitted-light Base Leica TL BFDF 38
Leica TL RC / Leica TL RCI 39
Leica IsoPro Manual Mechanical Stage: Assembly 40
Leica IsoPro Motorized Mechanical Stage: Assembly 43
Cables: Connections 47
Cables: Cable Duct 48
Cables: Diagram 49
Leica LED5000 MCI 50
Leica LED5000 MCI: Alternative Assembly 51
Leica LED5000 RL: Assembly 52
Leica EL6000 – Safety Notes 54
Leica EL6000 – Preparations 57
Leica EL6000 – Connection to the Fluorescence Lamp Housing 60
Leica EL6000 – Replacing the Lamp 62
Leica EL6000 – Using the Internal Shutter 63

Leica M Series User Manual 10
Quick Start Guide
The Fastest Route to Success 65
Overview of an M Series Microscope 66
The Correct Interpupillary Distance 67
Using the Eyepieces 68
Focusing 69
Adjusting the Resistance of the Focus Drive 70
Changing Magnification (Zoom) 71
Ratchet Steps and Magnification Levels 72
Parfocality: More Comfort and Convenience for Your Work 73
Iris Diaphragm 74
Eyepieces
Magnification Factors of the Eyepieces 76
Health Notes 77
Dioptric Correction 78
Dioptric Correction and Parfocality 79
Graticules 80
Photography & Video
Photography & Video 82
Photo Tubes and C-mounts 83
Trinocular Video/Phototube 50 % 84
Trinocular Video/Phototube 100 % 85
Trinocular Video/Phototube 100 %: ErgoTube 5°– 45° 86
Microscope Carrier
The Objective Nosepiece 88
Objectives and Optical Accessories
The Different Types of Objectives 90
Bases
Leica TL ST Transmitted-light Base: Controls 92
Leica TL ST Transmitted-light Base: Operation 93
Leica TL ST Transmitted-light Base: Changing Bulbs 94
Leica TL BFDF Transmitted-light Base: Controls 95
Leica TL BFDF Transmitted-light Base: Operation 96
Leica TL RC / Leica TL RCI: Controls 97
Leica TL RCI: The Deflection Mirror 98
Leica TL RCI: Color Intensity and Temperature 99
Leica TL RC / Leica TL RCI: Operation 100
Leica TL RCI: Methods in Transmitted Light 101
Leica TL RCI: Relief Images 102
Using Filters 104
Leica IsoPro (Non-motorized): Controls 105
Leica IsoPro (Motorized): Controls 106
Contents (Continued)

Leica M Series User Manual 11
System Illumination
Leica LED5000 MCI 108
Leica LED5000 RL 110
Leica EL6000 – About the Instrument 111
Leica EL6000 – Operation 113
Leica EL6000 – Troubleshooting 114
Cleaning and Maintenance 115
Fluorescence
Filter Changer 117
FIM – Fluorescence Intensity Manager 118
About Fluorescence Microscopy (Only Applicable for Leica M205 FA) 119
Rapid Filter Changers and Filter Types 120
Simple Filter Holders 121
Equipping the Filter Changer 122
Observation Without Fluorescence 123
Double Iris Aperture 124
Commissioning the Fluorescence System 125
Accessories
Leica Hand Wheel and Foot Switch 127
Leica SmartMove 128
Leica SmartTouch 129
Leica PSC Controller 130
Dimensional Drawings
Leica M165 FC 132
Leica M205 FA 135
Leica M205 FCA 138
Leica LED5000 MCI 141
Leica LED5000 RL 142
Leica MATS TL 143
Specifications
Leica M165 FC / Leica M205 FA / Leica M205 FCA 145
Leica EL6000 – Specifications 149
Leica TL ST Transmitted-light Base 151
Leica TL BFDF Transmitted-light Base 152
Leica TL RC / Leica TL RCI 153
Leica TL5000 Ergo 154
Leica IsoPro Motorized XY Stage 155
Appendix
Calculating the Total Magnification and Field of View Diameter 157
Care, Maintenance, Contact Persons 158
Contents (Continued)

Leica M Series User Manual The Leica M Series for Fluorescence 12
The Leica M Series
for Fluorescence

Leica M Series User Manual The Leica M Series for Fluorescence 13
Congratulations!
Congratulations on obtaining your new Leica M series stereo microscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
The Leica M series embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the M series
adapts perfectly to your needs – no matter which accessories you require
for your tasks.
The entire imaging system, including the zoom, objective and ErgoTube®,
is apochromatically corrected with much technological effort. Contrast,
sharpness, richness in detail, resolution, image and color fidelity are opti-
mum. In addition, the patented illumination beam path guarantees at
every zoom level that light utilization is at a maximum and that fluores-
cence images are intensely luminous on a jet black background.
Though the reliability and robustness of Leica stereo microscopes is
legendary, like any high-tech product, the Leica M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereo microscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work – after all, you are now
equipped with the best tool!

Leica M Series User Manual The Leica M Series for Fluorescence 14
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereo microscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engi-
neers have worked to push magnification, reso-
lution and image fidelity to the limit permitted
by optics.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimen-
sional image seen by the observer becomes
distorted – a sphere becomes an ellipse, a flat
surface curves towards the observer.
Limits are made to be broken.
The Leica M205 FA is the world's first fluores-
cence stereo microscope with a zoom range
of 20.5:1. This accomplishment, however,
was not enough for Leica's engineers. With
the new FusionOptic in the Leica M205 FA /
M205 FCA, they have succeeded in going yet
another step beyond previous limits. In addition
to the increase in magnification, the resolution,
too, has been increased to up to 1,050 lp / mm,
which corresponds to a resolved structure size
of 472 nm.
Of course, this increase in performance benefits
your everyday work. Set up your specimens
on the microscope table with comfortable
freedom of movement and discover details in
stereo microscopy that you could never see
before.
* Leica M205FA with 1.0× planapochromat and 10× eyepieces

Leica M Series User Manual The Leica M Series for Fluorescence 15
The Electronics: Comfort, Convenience and Safety for Your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new
M series lineup. Optics carrier, stand, base and
illuminator are all connected using electrical
contacts – which provides a number of advan-
tages.
Reliability for your experiments
Exact reproducibility of results is an increas-
ingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnifica-
tion, the illumination, the position of the iris
diaphragm and more and transmits the infor-
mation to the Leica LAS software. Thus you
always know the conditions under which an
image was acquired.
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also trans-
ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables – this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply
the power.
Leica Application Suite (LAS) evaluates the trans-
mitted data and can restore the test situation
later.

Leica M Series User Manual The Leica M Series for Fluorescence 16
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibil-
ity in choosing equipment, thanks primarily to
the modular configuration and the compatibil-
ity that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ signif-
icantly. Whichever configuration you choose,
you will quickly feel right at home.

Leica M Series User Manual The Leica M Series for Fluorescence 17
Leica engineers were careful to ensure that
the new Leica M series – like its predecessors –
remains compatible with existing series. This
means that objectives, bases, tubes and so on
can be reused.
Objectives
All new objectives of the Leica M series are
parfocal, meaning that when used with the
objective nosepiece, they can be replaced while
keeping the specimen in perfect focus.
If you prefer, you can continue to use the previ-
ous Leica objective series. In this case, parfocal-
ity is no longer guaranteed.
Tubes
The interface between the optics carrier and the
tube has remained the same, so existing tubes
fit the new M series.The new tubes are designed
for eyepieces with field number 23, while the
predecessor models were only designed for field
number 21, resulting in a smaller object field.
Eyepieces
However, the new M series eyepieces have an
audible and tangible click to provide immedi-
ate feedback in case of accidental adjustment.
Maximum Compatibility

Leica M Series User Manual The Leica M Series for Fluorescence 18
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the instal-
lation instructions and go directly to the Quick
Start Guide on page64.
If, on the other hand, you are assembling
the microscope yourself, continue with the
"Assembly" chapter, which begins on page19.
For everything you need to know about the
correctuse offluorescence-relatedparts,referto
page116.

Leica M Series User Manual Assembly 19
Assembly

Leica M Series User Manual Assembly 20
The first step is to connect the focusing column
of the M series to the corresponding base.
Tools used
OHex socket screwdriver, 3 mm
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing column
2. Securely screw the focusing column to the
base using the six included screws.
Assembling the Focusing Column for TL Bases