LI-COR Odyssey XF User manual

Odyssey® XF
Operator’s Manual

CE Marking:
This product (model number 2802) is a CE-marked product. For conformity information, contact
LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales
office or distributor.
奥德赛的Fc/XF成像仪
Odyssey Fc Imager and Odyssey XF Imager
部件名称
Part Name
有毒有害物质或元素
Toxic and Hazardous Substances or Elements
铅
(PB)
汞
(Hg)
镉
(Cd)
六价铬
(Cr(VI))
多溴联苯
(PBB)
多溴二苯醚
(PBDE)
印刷电路板
(PCBs) X O O O O O
机电配件
(Electromechanical Parts) X O O O O O
光学
(Optics) X O X O O O
电缆和电线
(Cables and Wires) X O O O O X
金属部件
(Metal Parts) X O O O O O
塑料零件
(Plastic Parts) OOOOOO
电池
(Batteries) OOOOOO
本表格依据 SJ/T 11364 的规定编制
O = 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572 规定的限量要求以下
indicates that the content of the toxic and hazardous substance in all the Homogeneous Materials of the part is below the concentration limit
requirement as described in GB/T 26572
X = 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572 规定的限量要求
indicates that the content of the toxic and hazardous substance in at least one Homogeneous Material of the part exceeds the concentration limit
requirement as described in GB/T 26572
Page 3

Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The
safety of this product cannot be guaranteed if the product is used in any other way than is specified in this
manual. The Odyssey® XF Imager is intended to be used by qualified personnel. Read this entire manual before
using the Odyssey XF Imager.
Equipment Markings:
The product is marked with this symbol when it is necessary for you to refer to the
manual or accompanying documents in order to protect against damage to the product.
The product is marked with this symbol when a hazardous voltage may be present.
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT Information of importance to prevent procedural mistakes in the operation of the
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
Federal Communications Commission Radio Frequency Interference Statement
WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in
accordance with the instruction manual, may cause interference to radio communications. It has been tested
and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC
rules, which are designed to provide a reasonable protection against such interference when operated in a
commercial environment. Operation of this equipment in a residential area is likely to cause interference in
which case the user, at his own expense, will be required to take whatever measures may be required to
correct the interference.
Page 4

Notice
The information contained in this document is subject to change without notice. LI-COR MAKES
NO WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT
LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR PURPOSE. LI-COR shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are
reserved. No part of this document may be photocopied, reproduced, or translated to another
language without prior written consent of LI-COR, Inc.
Printing History
Publication Number 977-19888
Revised: 2022-04-06
Global Headquarters
4647 Superior Street • Lincoln, NE 68504
Toll free: 800-645-4267 • Fax: [phone] • [email protected] •licor.com/bio
Regional Offices
LI-COR Biosciences GmbH
Siemensstraße 25A • 61352 Bad Homburg • Germany
Phone: [phone] • Fax: [phone] • [email protected]
LI-COR Biosciences UK Ltd.
St. John’s Innovation Centre • Cowley Road
Cambridge • CB4 0WS
United Kingdom • Phone: [phone] • Fax: [phone] • [email protected]
LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors
LI-COR, Odyssey, MPX, FieldBrite, Empiria Studio, and IRDye are trademarks or registered trademarks of LI-COR, Inc. in the
United States and other countries. Third party trademarks, trade names, and product names may be trademarks or registered
trademarks of their respective owners. You may not remove or alter any trademark, trade names, product names, logo,
copyright or other proprietary notices, legends, symbols, or labels in the Software Product. This EULA does not authorize you
to use LI-COR’s or its licensors’ names or any of their respective trademarks.
LI-COR is ISO 9001:2015 certified. © 2022 LI-COR, Inc. All rights reserved. Specifications subject to change. The Odyssey XF
Imaging System, FieldBrite XT technology, and IRDye reagents are covered by U.S. patents, foreign equivalents, and patents
pending.
Page 5

Chapter 1: Installation and Safety Information
Safety Considerations 9
Laser Safety 9
Safety Interlocks 10
Placement in the Laboratory 11
Ambient Laboratory Conditions 11
Instrument Ventilation 11
Space Requirements 11
Level the Imager 11
Moving the Imager 11
Chemical Safety 12
Electrical Considerations 12
Power Cords and Disconnecting Power 12
Fuse Replacement 12
Chassis Ground Access Hole 12
Computer Connections and Networking 12
Chapter 2: System Overview
External Panels and Controls 13
Front Panel 13
Right-side and Rear Panels 15
Optical System Description 15
Imaging Drawer 17
Imaging Drawer Safety 17
Sample Placement 18
Sticking Imaging Drawer 19
Image Acquisition 19
Using Membranes 19
Using Gels 21
Software Overview 21
LI-COR® Acquisition Software for Image Acquisition 21
Empiria Studio® Software for Analysis 22
Detection Channel Summary 22
Odyssey® XF
Imager
Table of Contents
Page 6

Chapter 3: Maintenance
Routine Maintenance 23
Clean Imager 23
Clean Tray 23
Cleaning the Fan Filter 24
Front Panel Warning Light Indicates an Error 24
Desiccant Replacement 24
Desiccant Replacement Instructions 25
Obtaining Technical Support 27
Chapter 4: Appendix
Specifications 29
Embedded Firmware Source Code 30
Page 7

Chapter 1: Installation and Safety Information
Safety Considerations
Laser Safety
The Center for Devices and Radiological Health (CDRH) was established in October 1982, by the
U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical
devices and radiological health.
Manufacturers of products subject to performance standards under the Radiation Control for Health
and Safety Act of 1968 are required to furnish various reports to the CDRH.
The Odyssey XF Imager is certified as a Class I laser product. This means that hazardous laser
radiation is not emitted outside the instrument. Radiation emitted inside the Odyssey XF Imager is
confined within protective housings and external covers. A series of safety interlocks ensures that the
laser beam cannot escape during any phase of user operation.
One laser emits at 785nm and the other one at 685-690nm. The 785nm laser has a peak power rating
of 1.2 watt. The 685-690nm laser has a peak power rating of 0.5 watt. The lasers emit visible and
invisible laser radiation - direct exposure to either beam may cause eye damage. Laser radiation
from each laser uniformly illuminates the target area only, which is marked in the imaging tray
(Figure 3-1) that fits in the imaging drawer. There is no emission of laser radiation outside the
instrument under normal conditions.
The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation
performance standard 21, Code of Federal Regulations Chapter 1, Sub-chapter J). Compliance for
products marketed in the United States is mandatory. This product complies with CDRH radiation
performance standard 21 CFR Chapter 1, Sub-chapter J. Ce produit est conforme à la performance de
rayonnement CDRH chapitre CFR norme 1 Sous-chapitre J. The label that must be attached to laser
products marketed in the United States is Figure 3-1 and is located on the rear panel of the Odyssey
XF Imager (Figure 3-2), indicating compliance with CDRH regulations.
WARNING: Use of controls or adjustments or performance of procedures other than those specified
herein may result in hazardous radiation exposure.
AVERTISSEMENT: L'utilisation des commandes ou le réglage ou la performance des procédures
autrement qu'il a été indiqué dams le présent texte peut résulter en de dangereuses expositions aux
radiations.
Odyssey® XF
Imager
Page 9

Chapter 1: Installation and Safety Information
Figure 3-1. CDRH and IEC 60825-1:2014 regulation compliance
label. See Power Cords and Disconnecting Power on
page12 for more information about disconnecting power before
servicing.
Figure 3-2. (a) Location of CDRH compliance label
on the right side of the Odyssey® XF Imager. (b)
Access hole for chassis ground.
Note: The appearance of your instrument's
back panel may vary slightly from the one
shown.
Safety Interlocks
Where there is a potential for class 4 laser radiation behind a given panel, the label in Figure 3-4 is
affixed to the panel. The label shown in Figure 3-4 is affixed to the Odyssey XF in two locations; one
on top of the camera hood and one on the inner front access panel (Figure 3-3).
Figure 3-3. Location of class 4 laser warning label
located on (a) the camera hood under the top panels of
the Odyssey XF Imager enclosure and (b) on the inner
front access panel (do not open, no user-serviceable
parts inside).
DANGER - CLASS 4 VISIBLE AND INVISIBLE
LASER RADIATION WHEN OPEN
AVOID EYE OR SKIN EXPOSURE TO DIRECT
OR SCATTERED RADIATION
DANGER
DANGER - APPAREIL LASER DE CLASSE 4.
RAYONNEMENT LASER VISIBLE ET INVISIBLE EN CAS D’OVERTURE.
EVITER L’EXPOSITION DE L’OEIL OU DE LA PEAU
AU RAYONNEMENT DIRECT OU DIFFUS.
Figure 3-4. Class 4 laser warning
label.
The Odyssey® XF Imager has
two safety interlocks that
prevent exposure to laser
radiation when the imaging
drawer is opened during
operation. Do not attempt to
defeat these interlocks. The
drawer interlocks are located in
the back of the instrument.
Page 10

Odyssey® XF Imager
Placement in the Laboratory
The Odyssey XF Imager weighs approximately 27kg (60lbs). The instrument should be placed on a
laboratory bench that is sufficiently sturdy to bear its weight. The bench should also be able to bear
the weight of the computer if the computer is to be installed beside the Odyssey XF Imager.
Ambient Laboratory Conditions
Place the Odyssey XF Imager away from external heat sources (furnaces, windows, etc.). Additional
heating can cause high temperatures within the enclosure. Place the instrument away from sinks or
other sources of water that pose a shock hazard. Recommended operating conditions are 15-35°C
and a dew point not greater than 22°C to prevent condensation on the laser assemblies, which are
cooled during operation.
Instrument Ventilation
The air intake fan on the back of the Odyssey XF Imager (Figure 4-4) is used to cool the internal
electronics and maintain fresh air circulation throughout the internal volume of the instrument. The
air intake is filtered and the filter should be periodically cleaned (see Maintenance on page23). Make
sure there is plenty of room behind the instrument so that the air intake is not blocked and the filter
is easily accessible for cleaning.
Space Requirements
The Odyssey XF Imaging System requires an area 41.4 cm W (16.3") × 47 cm D (18.5") × 67.3 cm H
(26.5"). Depth with the imaging drawer open is 59.7cm (23.5”).
Level the Imager
Level the imager to prevent the imaging drawer from scraping on anything and to keep the imager
from tilting.
The instrument can be leveled by turning the threaded metal inserts located in each of the 4 plastic
feet (Figure 3-5).
Figure 3-5. Turn feet to adjust height.
Moving the Imager
LI-COR recommends that you do not move your imager. Instead, please contact LI-COR Technical
Support for options.
LI-COR cannot be held responsible for any damage incurred while moving, transporting, or
shipping the Odyssey XF Imager, even if an existing warranty and/or service contract applies.
LI-COR cannot be held responsible for any injury incurred while moving, transporting, or
shipping the Odyssey XF Imager.
Page 11

Chapter 1: Installation and Safety Information
Chemical Safety
LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory
procedures be followed at all times. Be aware of the hazards associated with any chemical before you
begin work.
The LI-COR® Odyssey® XF Imager should not be used with any radioactive materials.
Electrical Considerations
Power Cords and Disconnecting Power
The Odyssey XF Imager is equipped with a 3-wire grounding-type plug. This plug will only fit into a
grounding-type outlet. This is a necessary safety feature. If you are unable to insert the plug into the
outlet, you will need to replace the outlet. Do not defeat the purpose of the grounding-type plug.
Do not locate the Odyssey XF Imager where the power cord will be walked on or exposed to water or
chemical spills.
The Odyssey XF Imager draws a maximum of 4 amperes at 120V (see Specifications on page29). If an
extension cord is used, make sure the total of the ampere ratings on the instruments plugged into the
extension cord does not exceed the extension cord ampere rating. Also make sure the total amperage
of instruments plugged into the wall outlet does not exceed the amperage capacity for the outlet
(usually 15 or 20 amperes in the United States).
Disconnect power before servicing.
Débrancher le pouvoir avant d'entretenir.
See Figure 3-1 on page10 for the location of these statements on the product's regulation compliance
label.
Fuse Replacement
There are no user replaceable fuses in this instrument.
Chassis Ground Access Hole
To eliminate the potential for ESD damage during service, a hole on the side of the control module
provides a chassis ground (Figure 3-2).
Computer Connections and Networking
Note: Use only the supplied Cat. 5e RJ45 Ethernet cable to connect the Odyssey XF Imager and
computer.
When LI-COR® Acquisition Software starts, it searches for and discovers any Odyssey XF Imagers
automatically. If only one instrument is found, LI-COR Acquisition Software establishes
communication. If more than one instrument is found, the user is presented with a list of
instruments and asked which to use.
Page 12

Chapter 2: System Overview
External Panels and Controls
Front Panel
Figure 4-1. Front panel features and controls of the Odyssey XF Imager.
Power On/Off Button: Pressing this button momentarily turns the instrument on and off. While
the instrument is performing its startup or shutdown procedure, the blue indicator light in the power
button blinks. When the blue indicator light is continuously on, the instrument is ready for
operation. Pressing the power on/off button when the green image acquisition indicator is lit will
Odyssey® XF
Imager
Page 13

Chapter 2: System Overview
cause the red error condition indicator to illuminate. This is just a warning that the instrument is in
use.
Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is
continuously on at other times, as long as communication with LI-COR® Acquisition Software is
maintained. The indicator light is off only if communication is lost or LI-COR® Acquisition
Software is closed or the instrument is powered off.
Figure 4-2. Location of the green image acquisition light and red error light.
Red Error Indicator: The error indicator light illuminates when a particular process could not be
completed. If the imaging drawer button is pressed before an acquisition is complete, for example,
the error indicator illuminates. See LI-COR® Acquisition Software for details of what error occurred.
Imaging Drawer Button: This button opens and closes the imaging drawer in normal operation.
The drawer front can be used to open or close the drawer in cases of power loss. Pressing the Imaging
Drawer button when the green image acquisition indicator is lit will cause the red error condition
indicator to illuminate. This is just a warning that the instrument is in use.
Imaging Drawer: The imaging drawer moves the imaging tray into position under the camera for
imaging. The bottom of the imaging tray has a marker in each corner that indicates the edge of the
camera’s field of view.
Page 14

Odyssey® XF Imager
Right-side and Rear Panels
Figure 4-3. Close up of the Odyssey XF Imager control
module showing the power receptacle and Ethernet
connectors (use only the supplied Ethernet cable). Do
NOTuse any other connector on this panel.
Note: The appearance of your instrument's
back panel may vary slightly from the one
shown.
Figure 4-4. Rear panel showing air intake fan.
Keep the intake unblocked during operation and
accessible for routine filter cleaning (see
Cleaning the Fan Filter on page24).
Optical System Description
The Odyssey XF Imager is designed to image Western blots, membranes, or gels using visible, near-
infrared or chemiluminescent methods. Before image acquisition starts, the imaging tray containing a
membrane or gel is placed in the Odyssey XF imaging drawer and the drawer is closed. A safety
interlock prevents operation of the laser illumination module while the imaging drawer is open.
Image acquisition is started via your acquisition software.
The Odyssey XF Imager has four detection channels: two are infrared channels with 685 and 785 nm
lasers, one is a 600 nm channel with a light source for excitation, and the fourth is for detecting
visible wavelength emissions from chemiluminescent substrates. The channels used during
acquisition are chosen in your image acquisition software.
Page 15

Chapter 2: System Overview
The Odyssey® XF Imager laser module contains a 685 nm and 785 nm laser. During image
acquisition, each laser source is turned on, followed by image acquisition with the LI-COR® CCD
camera. For infrared imaging, a typical image acquisition consists of images for two fluorescent
probes in the 700 and 800 nm channels (assuming both channels are enabled). Image acquisitions
from chemiluminescent substrates use just the chemiluminescence channel and imaging is done at
visible wavelengths. Note, however, that fluorescent markers can be imaged separately in the 700 nm
channel and combined with the image from the chemiluminescence channel. Coomassie stains can
also be imaged in the 700 nm channel. DNA gels stained with ethidium bromide or other
comparable stains (such as SYBR Safe) can be imaged in the 600 nm channel.
The quality of images from the Odyssey XF Imager is enhanced by a patented filtering system that
dramatically reduces noise before detection by the CCD detector. Infrared signal detection has also
been optimized for LI-COR® IRDye® near-infrared dyes, which eliminates the need for filter
selection by the user before imaging. The unique imaging technology, FieldBriteTM XT, used in the
Odyssey XF Imager acquires images without saturated pixels on the first attempt with no user
adjustments. More than six logs (22 bits) of dynamic range are available for each image.
Page 16

Odyssey® XF Imager
Imaging Drawer
The imaging drawer is opened and closed by pressing the large button above the drawer (see Figure
4-1).
Figure 4-5. The imaging drawer holds one imaging tray that is capable of containing one membrane or gel up to 10 x
12 cm.
Imaging Drawer Safety
Be careful not to get your fingers caught between the Odyssey XF Imager and the imaging drawer
when the drawer closes. The drawer is specially designed to stop when a certain level of resistance is
detected, so no harm should result, but the experience may be startling.
During an unexpected power loss, the drawer button will not function. This may leave a sample
inside the Odyssey XF Imager, or it may result in the imaging drawer being left partially open or
closed. If the power remains off, the drawer can be opened or closed by pulling or pushing on the
drawer front. The imaging drawer should resume normal operation once power is restored.
Page 17

Chapter 2: System Overview
Sample Placement
The Odyssey® XF Imaging Tray has four corner markers that delineate the edges of the Odyssey XF
imaging area. Place membranes or gels up to 10 x 12 cm on the imaging tray within the corner
markers for the entire membrane or gel to be imaged.
The imaging tray is designed to contain the small volume of liquid needed to keep blots or
gels moist. Do not fill the imaging tray and be careful not to add so much liquid that the
sloshing action created by closing the drawer may result in a spill inside the instrument.
Figure 4-6. Imaging Tray.
Place membranes sample-side up with the top toward the rear of the imager.
Place gels sample-side up with the top toward the rear of the imager.
Trays can be cleaned (Clean Tray on page23), but they should be discarded if an unacceptable
amount of residual signal remains on the tray after cleaning. Trays can be reordered using P/N 926-
40020 (5pk).
Insert Imaging Tray into Drawer
The imaging tray is inserted in the imaging drawer by aligning the notches in the tray with the guide
pins in the imaging drawer (Figure 4-7), and lowering the tray into the drawer. Make sure the tray
rests flat on the imaging drawer before pressing the open/close button to close the drawer.
Page 18

Odyssey® XF Imager
Figure 4-7. Imaging drawer has four guide pins that match the alignment notches in the imaging tray.
Clean Tray
Trays may be discarded after use. Trays should be discarded if they are damaged or if an unacceptable
level of residual signal remains after cleaning.
The following procedure may allow you to clean the tray sufficiently for reuse. If you choose to reuse
the tray, it should be cleaned before and after each use.
1. Remove sample from tray.
Always handle membranes with forceps.
2. Clean tray with water and wipe with a lint-free wipe.
Do not use blue wipes.
3. Rinse with 70% ethanol or 70% isopropanol and lint-free wipes. Methanol may be used if
necessary.
Do not use acetone or any other harsh solvents to clean the imaging tray.
Sticking Imaging Drawer
If the Odyssey XF imaging drawer does not open and close smoothly, contact LI-COR technical
support for instructions about acquiring the correct lubricant and correctly applying the lubricant.
Image Acquisition
Using Membranes
For Western blotting methods, nitrocellulose or PVDF membranes may be used (see
licor.com/membranes for the latest membranes and kits that are optimal for infrared detection).
Page 19

Chapter 2: System Overview
Detailed blotting protocols can be found at licor.com/support. These are some general tips for using
membranes.
Handling Near-Infrared Western Blot Membranes
lImportant: Do not touch the membrane – handle only with clean forceps. Lift the membrane
only by the corners. Fingerprints, even from a glove, will show clearly when imaged.
lUse clean containers to avoid cross-contamination and reduce background.
lProtect near-infrared (NIR) Western blot membranes from light prior to imaging.
lKeep the membrane wet if it is to be stripped and re-used.
lStore in PBS or TBS at 4 °C or store dry at room temperature. The fluorescent signal on a dried
membrane will remain stable for several months or longer if protected from light and air.
Acquiring NIR Western Blot Images
NIR Western blot membranes can be imaged wet or dry. Place the membrane sample-side up in the
imaging tray, and place the imaging tray into the imaging drawer.
LI-COR Acquisition Software Steps for Scanning Membranes
1. Start LI-COR Acquisition Software .
2. Click Scan .
3. Enter a Username.
4. Choose your Odyssey® XF in the list of imagers.
5. Choose the Membrane workflow and follow the steps in the workflow to acquire an image.
Handling Chemiluminescent Western Blot Membranes
lImportant: Do not touch the membrane – handle only with clean forceps. Lift the membrane
only by the corners. Fingerprints, even from a glove, will show clearly when imaged.
lUse clean containers to avoid cross-contamination and reduce background.
lDo not dry chemiluminescent membranes for imaging.
Acquiring Chemiluminescent Western Blot Images
Image chemiluminescent membranes wet and with enough substrate evenly dispersed.
LI-COR Acquisition Software Steps for Scanning Membranes
1. Start LI-COR Acquisition Software .
2. Click Scan .
Page 20