Macherey-Nagel NANOCOLOR VIS II User manual

Smart photometry
NANOCOLOR®VIS II
NANOCOLOR®UV/VIS II
Quickstart guide
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
France:
MACHEREY-NAGEL EURL
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
Switzerland:
MACHEREY-NAGEL AG
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
Germany
and international:
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
MACHEREY-NAGEL
EN ISO 9001: 2008
CERTIFIED
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
France:
MACHEREY-NAGEL EURL
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
Switzerland:
MACHEREY-NAGEL AG
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
Germany
and international:
Tel.: [phone]
Fax: [phone]
E-mail: [email protected]
MACHEREY-NAGEL
EN ISO 9001: 2008
CERTIFIED
Distributed By:

3Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
1. Introduction.......................................................................................5
1.1 Technical description.............................................................................................5
1.2 Technical details....................................................................................................6
2. Safety precautions ...........................................................................8
2.1 Input supply...........................................................................................................8
2.2 Biological risk ........................................................................................................ 8
2.3 Protective clothing................................................................................................. 8
2.4 Improper handling and warranty ...........................................................................8
2.5 Damage of casing ................................................................................................. 8
2.6 Damage of cable................................................................................................... 9
2.7 Transport...............................................................................................................9
2.8 Lamps ...................................................................................................................9
3. Instrument set-up ...........................................................................10
3.1 Set-up location .................................................................................................... 10
3.2 Package content .................................................................................................10
4. Outer appearance...........................................................................11
4.1 Front and side view............................................................................................. 11
4.2 Back view............................................................................................................ 12
5. Operating instruction.....................................................................13
5.1 Connectivity ........................................................................................................13
5.2 Turning on the instrument ...................................................................................13
5.3 Operation and user guidance.............................................................................. 14
6. Methods...........................................................................................19
6.1 Basic functions.................................................................................................... 19
6.2 MN tests.............................................................................................................. 23
6.3 Scan.................................................................................................................... 25
6.4 Color measurements........................................................................................... 26
6.5 Test number ........................................................................................................27
7. Data memory...................................................................................28
7.1 Measurement memory ........................................................................................ 29
7.2 IQC memory........................................................................................................ 30
7.3 Backup ................................................................................................................31
8. Service.............................................................................................32
8.1 Spare parts, accessories and consumables .......................................................32
8.2 Error messages................................................................................................... 32
8.3 Waste disposal....................................................................................................32
8.4 Cleaning the instrument ...................................................................................... 32
8.5 Contact................................................................................................................32

5Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
1. Introduction
Welcome and thank you for deciding on a spectrophotometer from MACHEREY-NAGEL.
The NANOCOLOR®UV/VIS II / VIS II is a powerful, fast and compact spectrophotometer for
water analysis, which is able to evaluate MACHEREY-NAGEL NANOCOLOR®tube and
standard tests. In addition, the instrument is also capable of determining the nephelome-
tric turbidity measuring of a sample by light diffusion at a 90° angle. The combination of a
10.1” HD display with an intuitive, icon based menu structure enables a fast, comfortable
and enjoyable handling of the spectrophotometer. Thus, the spectrophotometer is the
ideal laboratory photometer for the entire spectrum of water analysis.
1.1 Technical description
A halogen lamp and a deuterium lamp cover the wavelength range from 190–1100 nm
in the NANOCOLOR®UV/VIS II. A halogen lamp covers the wavelength range from
320–1100 nm in the NANOCOLOR®VIS II. The light of the respective lamp is diffracted
via a concave grating and sent through a slit into the instrument’s cuvette slot. After
the light has passed through the sample, the amount of absorbed light is calculated by
detecting the remaining light.
Matching the measured absorbed light with a pre-set calibration, the instrument can
calculate the concentration of the sought analyte.

6Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
1.2 Technical details
NANOCOLOR®UV/VIS II NANOCOLOR®VIS II
Type: Spectrophotometer with reference detector technology (RDT)
Light sources: Halogen lamp (visible range)
Deuterium lamp (UV range)
Halogen lamp
Optical system: Monochromator
Wavelength range: 190–1100 nm 320–1100 nm
Wavelength accuracy: ± 1 nm
Wavelength resolution: 0.1 nm
Wavelength calibration: Automatic
Wavelength selection: Automatic, barcode, manual
Scan speed: 900 nm / < 1 min, 1 complete
scan < 1 min
1 complete scan < 1 min
Spectral bandwidth: < 2 nm < 4 nm
Photometric range: ± 3.0 E in wavelength range
200–900 nm
± 3.0 E in wavelength range
340–900 nm
Photometric accuracy: 0.005 E bei 0.0–0.5 E; 1 % bei 0.5–2.0 E
Photometric linearity: < 0.5 % bei 2 E; ≤ 1 % bei > 2 E
Stray light: < 0.05 % < 0.5 %
Measuring modes: More than 200 preprogrammed tests and special methods,
100 user dened methods, absorbance, transmission, factor,
kinetics, 2-point calibration, scan, nephelometric turbidity
measurement
Turbidity measurement: Nephelometric turbidity measurement, 0.1–1000 NTU
Cuvette slot: Test tubes 16 mm OD
Rectangular cuvettes
2 mm, 10 mm, 20 mm, 40 mm and 50 mm
Data memory: 16 GB Micro SDHC card, 5000 measured data sets / spectra,
GLP conform
Display HD 10.1 inch, anti-reective cover glass with projected
capacitive touch screen (PCAP) and background LED lighting
Operation: Barcode technology, icon-based menu guidance, touch screen
Languages: DE / EN / FR / ES / PT / PL / HU
External light: Insensitive, open cuvette slot
Interfaces: LAN (CAT 6; only use shielded cable of max. 20 m length)
2 x USB (Host), 1 x USB (Function) and 1 x RS 232 (only use
shielded cable of max. 3 m length)
Update: Via Internet / PC and USB stick
Operating range: 10–40 °C, max. 80 % relative humidity (without condensation)
Power supply: 110–240 V, ~ 50/60 Hz 12 V, DC 3A

7Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
Fuse: 2A TL –
Dimensions (L / W / H): 400 / 440 / 170 mm 360 / 400 / 110 mm
Weight: 6.5 kg 4.0 kg
Warranty: 2 years
Housing protection type: IP20
Protection rating: class 1 class 3
Declaration of conformity: EMC directive 2014-30-EU; EN 61326-1:2006;
LVD directive 2014-35-EU; EN 61010-1:2010;
EN 61010-2-81:2002+A1:2003; EN 60529 (IP20)

8Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
2. Safety precautions
Please read the instruction manual carefully before setting up and using the instrument.
In case instructions are disregarded, the instrument may malfunction or get damaged. To
ensure perfect performance of the instrument, it may only be used as described in this
manual. Please pay particular attention to the following warning notices, which indicate
special dangers when using the instrument.
Caution:
Imminent danger of an electric shock.
!
Attention:
Indicates actions that pose a potential threat to instrument performance or may
cause instrument damage.
!
Warning:
Indicates actions that pose a threat to the user. In case of not adhering to the
advice, slight or serious injuries may occur.
Caution:
Indicates danger by laser radiation.
2.1 Input supply
!
To ensure safe functioning of the instrument, please use the included power
cable or power adapter only.
2.2 Biological risk
When using the instrument, it may be necessary to handle hazardous chemicals or
biologically harmful samples. Please adhere to the following guidelines while using the
instrument:
• Please read all information concerning hazards and safety measures in the material
safety data sheets and on the packages you work with.
• All utilized compounds need to be disposed in accordance with national rules and
regulations.
• The type of safety equipment utilized needs to be in line with the concentration and
hazard potential of the given substance used.
2.3 Protective clothing
As protective clothing we recommend to wear protection gloves and a laboratory coat.
2.4 Improper handling and warranty
In case of improper handling or handling operation by untrained personnel,
MACHEREY-NAGEL declines any warranty claims.
2.5 Damage of casing
If the housing is damaged, the instrument needs to be sent in for repair. In such a case,
the proper functioning of the instrument as well as the correctness of the measurement
results cannot be guaranteed anymore.

9Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
2.6 Damage of cable
In case of a damaged cable, switch off the instrument and replace the cable immediately.
2.7 Transport
For transporting the instrument, the cardboard box used for the initial shipment as well
as the protective inner parts are perfectly appropriate. Therefore, we recommend kee-
ping the box. In case the original box is not available anymore, a large cardboard box
and plenty of soft packing material, which prevents the instrument from shifting during
transport, can be used.
2.8 Lamps
!
The NANOCOLOR®UV/VIS II features a halogen and a deuterium lamp. The
NANOCOLOR®VIS II features a halogen lamp. Both get very hot during opera-
tion. Hence, there is a risk of severe burns. Before exchanging the lamps, let
them cool down for at least 30 minutes.
Please disconnect the instrument from the power source before starting any
maintenance work in order to avoid electric shock.
!
The deuterium lamp of NANOCOLOR®UV/VIS II generates ultraviolet radiation,
which can harm your eyes. Never look directly into the light source without sui-
table UV protection glasses. Also, protect your skin against direct UV light.
The barcode reader of NANOCOLOR®spectrophotometer is subject to laser
projection class 1M.
LASER RADIATION
DO NOT
VIEW DIRECTLY
WITH OPTICAL INSTRUMENTS
CLASS 1M LASER PRODUKT

10 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
3. Instrument set-up
3.1 Set-up location
!
Only use the instrument in a suitable location. It should be placed on a dry,
clean, leveled and horizontal surface. The operating temperature is between
10–40 °C. Make sure to protect the instrument from drastic changes in tempera-
ture. Avoid positioning the instrument close to windows, hot plates, ovens etc. To ensure
correct functioning and reliable results, the instrument should not be exposed to direct
sunlight or other bright, focused light sources. For optimal performance, humidity should
be between 20–80 %
3.2 Package content
Please open the shipping box carefully with a sharp tool. Make sure not to damage its
contents. Remove the instrument and all other parts carefully. Check the package, inst-
rument and accessories for visible damages. In case a part is damaged, please contact
your distributor or MACHEREY-NAGEL’s technical support (see 8.5 Contact, p. 32).
The following list contains all items inside the package. Please ensure that your ship-
ment is complete. In case of missing items, please also contact your local distributor or
MACHEREY-NAGEL’s technical support.
Note: Keep the original box as well as the packaging material from the initial shipment to
optimally protect the instrument in case of a return-shipment.
Package content
• NANOCOLOR®UV/VIS II or NANOCOLOR®VIS II
• Protective covering
• Power plug (only for NANOCOLOR®UV/VIS II)
• Power adapter (only for NANOCOLOR®VIS II)
• USB connection cable
• This quickstart guide
• Calibration cuvette
• Microber cloth
• USB stick
• Touch pen

11Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
4. Outer appearance
4.1 Front and side view
aa
b c
d
e
Fig. 1: Front view UV/VIS II
f
Fig. 2: Side view UV/VIS II
aa
bc
e
Fig. 3: Front view VIS II
f
Fig. 4: Side view VIS II
a Stereo speakers
b 10,1″ HD touch screen with PCAP
c Universal cuvette slot for tube tests of 16 mm OD and rectangular cuvettes of 2 mm,
10 mm, 20 mm, 40 mm and 50 mm
d Lamp compartment with halogen and deuterium lamp (SDHC card slot)
e 2D bar code scanner
f USB A interface

12 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
4.2 Back view
1 2 3 4
5 6
Fig. 5: Back view UV/VIS II
7 1 2 3 4 8 6
Fig. 6: Back view VIS II
1 USB A interface
2 USB B interface
3 Ethernet (LAN) interface
4 RS 232 interface
5 Connection for power supply 110-240 V (~ 50/60 Hz)
6 Main switch
7 SDHC card slot (UV/VIS II refer to Fig. 1d)
8 Connection for power supply 12 V DC 3A

13Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
5. Operating instruction
The NANOCOLOR®UV/VIS II requires a voltage of 110–240 V (~ 50/60 Hz). Plug
in the power cable into the connection for power supply (Fig. 5 5) on the
instrument’s back side.
The NANOCOLOR®VIS II requires a voltage of 12V (min. 3A). Plug in the pow-
er adapter into the power supply and establish the connection to power supply
(Fig. 6 8) on the instrument’s back side afterwards.
Then, plug the power plug into the electrical outlet. Please ensure that the power plug or
power adapter is intact with neither cable break nor other damages. Otherwise, there is
an imminent danger of an electric shock.
5.1 Connectivity
Next to the electrical connection (Fig. 5 5), 4 more types of interfaces are available
within the NANOCOLOR®UV/VIS II as indicated on page 10:
2 x USB Host (Fig. 5 1and Fig. 2 f)
1 x USB function (Fig. 5 2)
1 x Ethernet (LAN) (Fig. 5 3)
1 x RS 232 (Fig. 5 4for the connection of different devices as well as laboratory infor-
mation management systems (LIMS))
Next to the electrical connection (Fig. 6 8), 5 more types of interfaces are available
within the NANOCOLOR®VIS II as indicated on page 10:
1 x SDHC-card slot (Fig. 6 7)
2 x USB Host (Fig. 6 1and Fig. 4 f)
1 x USB function (Fig. 6 2)
1 x Ethernet (LAN) (Fig. 6 3)
1 x RS 232 (Fig. 6 4for the connection of different devices as well as laboratory infor-
mation management systems (LIMS))
5.2 Turning on the instrument
The main switch of NANOCOLOR®UV/VIS II (Fig. 5 6) / NANOCOLOR®VIS II (Fig. 6 6)
can be found on the instrument’s back side. Turn on the instrument. Subsequently, a boot
screen appears displaying the MACHEREY-NAGEL logo. Afterwards, the instrument
performs a self test which takes about one minute. Next, a pop-up informing about the
results of the performance check will be displayed. Press or to move to the start
screen. Now the instrument is ready to use. The instrument’s operating state can be
checked in the upper left corner of the display by pressing the status-icon. A green icon
indicates that the instrument is ready to use. A red icon indicates that the instrument is
not ready to use or for example performing a measurement.

14 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
5.3 Operation and user guidance
The spectrophotometer features a 10.1″ HD display with a projective, capacitive touch
screen (PCAP). The instrument’s cover-glass is non-reecting. It can be easily and
conveniently cleaned with the microber cloth included in your package or with a soft
cotton cloth.
UV/VIS II Open Mode, 28.10.2014, 10:57
Fig. 7: Start screen

15Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
5.3.1 Operation of the touch screen
The spectrophotometer features a 10.1″ HD display with a PCAP touch screen sensitive
to touches of one or more ngers. This enables handling by means of pressing or wiping
on the screen with either ngers or a touchpen (special pen for projective, capacitive
touch screens). Wearing protective gloves does not impair handling the instrument
properly.
In selected menus, you can zoom in and out by moving two ngers in opposite directions
or towards each other. Numbers or texts can be inserted to the respective number or text
elds, by touching the respective eld which automatically opens a pop-up displaying a
text and numeric keypads.
0
7
4
1
OK
8
5
2
9
6
3
.
+/-
C
0
Fig. 8: Numeric keypad
<-> CE
m ,.nb
a k !ljh
q i pouz
y :
123
s ?
w
x
d
e
c v
f g
r t
Fig. 9: Text keypad

16 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
5.3.2 Task and status bar
The spectrophotometer menu is equipped with a status information bar at the top and a
task bar at the bottom of the display.
Both bars are always displayed. The upmost bar displays the instrument’s operating
status.
UV/VIS II Open Mode, 28.10.2014, 10:57
Fig. 10: Status bar
In case of having activated special features (e.g. memory, LAN, etc.), a status icon ap-
pears. The status icon in the upper left corner displays whether the instrument is ready to
use. A green icon indicates that the instrument is ready to use. A red icon indicates that
the instrument is currently busy, e.g. with a measurement process.
The instrument can be operated via the task bar.
UV/VIS II Open Mode, 28.10.2014, 10:57
Fig. 11: Task bar
During the measuring process the bar is inactive (shaded appearance).
Task bar functions:
Home icon:
By pressing this icon at any position in the menu, one is guided back to the start
screen (see Fig. 7: Start screen, p. 14). From the start screen, it is not possi-
ble to get back to the last application via the icon.
Back icon:
By pressing this icon, one goes back to the application or level that was used
last.
Please consider that some menus cannot be retrieved by pressing the icon (e.g.
measurement menu), as the process has been ended by leaving the respective menu.
Feature icon:
By pressing this icon, one can activate additional features as long as they are
available in the selected menu.
Test icon:
By pressing this icon, basic features as well as MACHEREY-NAGEL tests and
applications, such as special methods, scan, color measurement or test number
can be selected in the method menu.
Main menu icon:
By pressing this icon, one can get to the main menu, including settings, the IQC
menu as well as other functions.
Memory icon:
By pressing this icon, the measurement memory is opened. The results of
measurements available via the icon are staved here.

17Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
5.3.3 Favorites bar
The instrument allows you to add favorites to the start screen to personalize the instru-
ment and to provide quick access to the most preferred functions. Initially, the favorites
bar features the icon for NANOCOLOR®tube tests. Other icons can be added to the
favorite bar by holding and moving them to the blue colored area. Removal of icons from
the favorites bar is done by holding an icon and moving it out of the blue colored area.
UV/VIS II Open Mode, 28.10.2014, 10:57
Fig. 12: Editing the favorites bar
5.3.4 Option buttons and check boxes
Option buttons are framed in black. Activated option buttons are lled with a black dot.
Please consider that only one selection point can be activated at a time.
You can activate a selection point by touching it. By leaving the menu, the selected
options will be saved automatically.
ü
Check boxes are square shaped. Touching an empty check box or its text will set a
checkmark and activate the box. It is possible to select more than one check box at once.
Touching a checkbox with a checkmark deactivates the box and removes the checkmark.

18 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
5.3.5 List functions
When editing text or numeric elds, it is possible that a selection window with a list will be
opened instead of a keyboard. This list can be scrolled down by nger movement on the
display. A suitable eld can be selected by touching it. The background of the currently
selected entry will be colored. There are two kinds of lists: pre-dened lists (e.g. choice of
region) cannot be edited and complemented, while dynamic lists can be adjusted.
UV/VIS II Open Mode, 28.10.2014, 10:57
M
10
Sept
1:x
1+x
Settings
Power saving mode
Dilution Format
Backup
Sipper
NTU Check
Lamp Settings
Reaction Time
Remember Sample
Place
Printer
Sound
Memory Settings
Date & Time
Region
Language
Select region
AR (Argentina)
AT (Österreich)
AU (Australia)
BE (Belgique)
BE (België)
BG (БЪԓгариЯ)
Fig. 13: Predened list
UV/VIS II Open Mode, 28.10.2014, 10:57
0.0
0.0
Standard Measurement
OK
Standard
Method
Submethod
Cuvette
Setpoint
Margin
Standard LOT
Test LOT
Select standard
0-04 Ammonium 10 (REF 925 011)
0-05 Ammonium 50 (REF 925 012)
0-06 Ammonium 200 (REF 925 013)
0-07 AOX 3 (REF 925 07)
0-08 Ammonium 100 (REF 925 013)
0-09 Lead 5 (REF 925 016)
+
Fig. 14: Dynamic list
A new entry can be added by pressing +at the upper right corner of a dynamic
list. A numeric or text keypad will appear in order to add the new entry. Conrming the
entry will add it to the list. Entries can only be deleted in dynamic lists by pressing the
respective entry for a moment until a recycle bin appears. Pressing will remove the
entry from the list.

19Quickstart guide NANOCOLOR®UV/VIS II / VIS II V 1.02 / 08.15
6. Methods
UV/VIS II Open Mode, 28.10.2014, 10:57
1 2 3
4 5 6
7 8 9
Test Number
Methods
Basis Functions MN Tests Special Methods Scan Color
Fig. 15: Methods
6.1 Basic functions
6.1.1 Factor
xF
The basic function “Factor” enables the determination of measurement results
through the multiplication of an absorbance with a user dened factor. After
having selected this method, a window will appear asking for the factor and the
wavelength for the measurement and calculation.
Conrming with opens the measurement window. Inserting the zero solution and
pressing will initiate the zero measurement. After the successful measurement of
the zero solution, the sample solution needs to be inserted. Pressing will start the
measurement process. After the measurement has been completed, the measurement
result will be displayed in the result window.

20 Quickstart guide NANOCOLOR®UV/VIS II V 1.02 / 08.15
6.1.2 Standard
c = Ex
cs
Es
c = Ex
cs
The basic function “Standard” enables the denition of a sample’s concentration
on basis of the concentration of a standard solution and a zero solution. After
having selected this method, a window will appear asking for the standard con-
centration and the wavelength for the measurement.
Conrming the entries by pressing will open the measurement window. Inserting the
zero solution and pressing will initiate the zero measurement. Then, the standard
solution needs to be inserted. Pressing will start the sample measurement.
After the standard solution the sample solution has to be inserted. Pressing will
start the sample measurement. After the measurement has been completed, the mea-
surement result will be displayed in the result window. In case more samples need to be
investigated the next sample can be inserted. Pressing will start the measurement
of the next sample.