NuAire 13 Series User manual

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Allergard Class I
Animal Bedding Disposal Cabinet
Models:
NU-607-400 (Series 13)
NU-607-400E (Series 15)
Operation and Maintenance Manual
February, 2019
Revision 16
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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Allergard Class I
Animal Bedding Disposal Cabinet
Models
NU-607-400 / NU-607-400E
Operation and Maintenance Manual
Table of Contents
Section No. 1...................................................................................General Description
Section No. 2...................................................................................Models and Features
Section No. 3...................................................................................Warranty
Section No. 4...................................................................................Shipments
Section No. 5...................................................................................Installation Instructions
5.1....................................................................................................Location
5.2....................................................................................................Set-Up Instructions
5.3....................................................................................................Assembly Members/Parts
5.4…………………………………………………………………Electrical Services
5.5…………………………………………………………………Assembly Instructions
5.6....................................................................................................Certification Testing Methods and
Equipment
Section No. 6...................................................................................Operating the NU-607
6.1…………………………………………………………………Operator Controls & Indicators
6.2....................................................................................................Flow Gard Operation
6.3…………………………………………………………………Operating Guidelines
6.4…………………………………………………………………Bag Installation and Removal
6.5.....................................................................................................Ergonomics
6.6.....................................................................................................Cleaning Procedures
Section No. 7...................................................................................General Maintenance
7.1....................................................................................................Decontamination
7.2....................................................................................................Fluorescent Lamp Bulb Replacement
7.3....................................................................................................HEPA Filter Replacement
7.4....................................................................................................Motor/Blower Replacement
7.5....................................................................................................Airflow Calibration
7.6....................................................................................................Filter Integrity Check
Section No. 8...................................................................................Polycarbonate Material Compatibility
Section No. 9……………………………………………………... Electrical/Environmental Requirements
Insert………………………………………………………………Replacement Parts List
Standard Drawings
BCD-03692..................................NU-607 Airflow Schematic
BCD-03693..................................NU-607 Spec. Drawing W/Worksurface Disposal Funnel
BCD-03706..................................NU-607 Spec. Drawing W/Worksurface Disposal Chute
BCD-04453..................................NU-607 Spec. Drawing W/Worksurface Disposal Double Chute
BCD-03691..................................NU-607 Assembly Instructions
BCD-04102..................................NU-607 Light Panel & Hinged Viewing Window Installation
BCD-03690..................................NU-607 Service Access Features
BCD-03689..................................NU-607 Exploded Assembly of Blower Module
BCD-03586..................................NU-607 Electrical Schematic (115VAC)
BCD-03625..................................NU-607 Electrical Schematic (230VAC)

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Allergard Class I
Animal Bedding Disposal Cabinet
Models:
NU-607-400/NU-607-400E
Operation and Maintenance Manual
MANUFACTURED BY:
NuAire, Inc. - Plymouth, Minnesota
1.0 General Description
The Allergard Model NU-607 Class I Animal Bedding Disposal Cabinet is a product resulting from the latest
design innovations, highest quality and newest product features that the Animal Research Professional
demands. The NU-607, with the spacious 14-inch (356mm) work access opening, provides personnel protection
for handling low to moderate risk material, minimizing the risk of exposure to allergens and animal infections
for the laboratory staff. Because a Class I cabinet allows room air to flow over the work surface, the cabinet
does not provide a sterile environment for cage cleaning activity. In order to facilitate varying sizes in cages,
three work surface configurations are available; small animal cages (funnel or double chute) and large animal
cages (single chute).

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2.0 Models & Features
The NU-607 is manufactured in one size. 4 ft. (1.2m)

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3.0 Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
within the data packet sent with the unit.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your Allergard cabinet arrives without damage. Motor
carriers are carefully selected and shipping cartons have been specially designed to insure your purchase.
However, damage can occur in any shipment and the following outlines the steps you should take on receipt of
a NuAire Allergard cabinet to be sure that if damage has occurred, the proper claims and actions are taken
immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before
acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the
consignee’s copy of the freight bill. Then an inspection must be made to verify the claim against the
carrier. This inspection is the basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and
request an inspection. Without this inspection, the transportation company may not accept a claim for
loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that
he was contacted on a certain date and that he failed to comply with the request. This, along with
other papers in the customer’s possession, will support the claim.

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5.0 Installation Instructions
5.1 Location
Within the laboratory, workspace, etc., the ideal location of the NU-607 is away from personnel traffic lanes, air
vents (in or out), doors and/or any other source of disruptive air currents.
SUGGESTED CABINET LOCATION IN LABORATORY
If drafts or other disruptive air currents exceed the intake velocity of the cabinet through the access opening,
the potential exists for contaminated air to exit the cabinet. It depends on the severity of the air current.
Remember, a containment hood is not a substitute for good laboratory technique.
For proper cabinet ventilation, the rear of the NU-607 should be placed no closer than 6 inches (152mm) from
the wall or any object that would impede the exhaust air. The airflow monitor will, however, alert the operator
if insufficient air is being exhausted (or work access inflow velocity).
5.2 Set-Up Instructions
The NU-607 may be shipped knocked down, in a specially designed carton, placed on a base skid. It is usually
best procedure to leave the unit in its carton until it is located in the laboratory in order to minimize lost or
misplaced assembly parts and to facilitate ease of handling. The cabinet is held to the base skid via banding
which can be cut easily with a scissors or a knife. The cabinet is shipped in three major parts:
1) Base cabinet including waste receptacles and work surface,
2) The HEPA/sorbent filter module (one piece), and
3) The workspace walls and hardware.
All carton and packing material should be thoroughly inspected before being discarded to insure no assembly
parts are accidentally thrown away or any hidden shipping damage has occurred.

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5.3 Assembly Members/Parts
The cabinet is assembled per Drawings BCD-04102 and BCD-03691. Before beginning assembly, make sure that
all parts are accounted for as follows:
1) White acrylic back panel; 1 each
2) Clear acrylic side panels; 2 each
3) 2 each outside trim angles
4) 2 each inside trim angles
NOTE: The inside trim angle has protruding studs on the angle that are inserted through the holes in the
acrylic walls.
5) 4 each, sets of hardware (washer, lockwasher, and nut).
6) Polycarbonate view screen with light shield attached, and
7) 4 each, sets of #8-32 screws.
5.4 Electrical Services
The NU-607 is provided with an electrical power cord. The station requires 115 VAC, single phase
power and is recommended to be on its own branch circuit, protected with a 15 Amp circuit breaker at
the distribution panel.
PLEASE NOTE, THIS UNIT CONTAINS AN ELECTRONIC BALLAST FOR THE FLUORESCENT LIGHTING.
ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE
THIS PRODUCT WITH GROUND FAULT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE
THE GFCI TO TRIP.

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5.5 Assembly Instructions
The following steps should be followed in order to insure proper assembly of the unit.
Step 1: Assemble the acrylic walls. Starting with the rear and one side panel, insert the inside trim angle
through the holes provided and attach outside trim angle. Leave the corner angles slightly loose so
the sidewalls can be moved forward to backward.
Step 2: Using the lift handles, place the HEPA clean air filter module on top of the acrylic sidewalls and into
the slots provided for on the module.
NOTE: It is recommended that at least two people (one on each side) lift the module onto the wall.
Step 3: Put the stainless steel work surface in place as shown on BCD-03691; fasten the hinges to the back
panel. Fasten work surface support spring.
Step 4: Install the light panel and clear acrylic viewing window as shown in Drawing BCD-04102.
Note: It may be more convenient to remove the light panel from the viewing screen before
attaching to the module.
a. Install light panel to blower module with hardware shown.
b. Attach viewing window to light panel with adjustable hinges using hardware shown.
c. Install a No. 8 screw and flat washer (2 places) one on each end of light panel.
Step 5: With the viewing window resting on the sidewalls, check to insure that the window lays flat on both
sidewalls. If not, gently slide either one backward or forward to insure a close tolerance fit.
Step 6: Tighten the hardware around trim angles.
Step 7: The charcoal filter is protected from sorption during shipment by a plastic covering, which must be
removed before the unit will function properly (see Section 7.3 for filter removal).
Step 8: Remove the protective covering from the acrylic sidewalls. The exterior acrylic surfaces are easily
cleaned with any mild household detergent cleaner, using a soft non-abrasive cloth. Harsh
chemicals, solvent type cleaners should not be used. Never use a polish to remove scratches from
the acrylic.

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5.6 Certification Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be certified or commissioned to factory
standards. At a minimum, the following test should be performed.
1. HEPA filter leak test
2. Airflow velocity
3. Airflow smoke patterns
Of these tests, in order to insure that no disruptive air currents are penetrating the air inflow barrier smoke flow
tests must be performed. These tests must result in the containment of smoke passed around the perimeter of
the work access opening. A smoke source shall be passed along the entire perimeter of the work opening
edges, approximately 1.5 inches (38mm) outside the cabinet. The smoke shall show a smooth inward flow with
no escape from the work access opening.
IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR
WITH THE METHODS AND PROCEDURES FOR CERTIFYING SAFETY CABINETS.
AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY
TIME THE OPERATOR FEELS IT IS NECESSARY.
Note that NuAire cabinets, filters and seals provide premium performance; Quality Control in both design and
manufacturing assure superior reliability. However, protection to the operator is so vital that certification to
the performance requirements should be accomplished as stated to insure safety established by the factory
standards.

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6.0 Operating the NU-607
6.1 Operator Controls and Indicators
The following is a description of the controls and indicators, (see location of control panel on Drawings
BCD-03693 and BCD-03706).
6.1.1 Airflow Control
Airflow inside the unit is controlled by a Potentiometer behind a chrome pop-out button that is located
on the instrument panel. To increase airflow, turn the airflow adjustment clockwise and
counterclockwise to decrease airflow.
The motor speed control should never be tampered with, except by a qualified technician who can
measure airflow through the work access opening with a suitable velometer. Note that the motor/fan
system is designed to minimize any requirement for the adjustment of motor speed as the HEPA filter
loads up with contaminants.
6.1.2 Blower Switch
The blower switch applies power to the internal blower/motor when in the ON position.
6.1.3 Power “ON” Light
A green neon light is located above the blower on/off switch and lights when power is applied to the
motor blower. This switch also powers the Airflow Monitor.
6.1.4 Circuit Breaker
The entire cabinet is protected with a circuit breaker. The circuit breaker, in conjunction with the
motor’s thermal protector, is designed to open under locked rotor or half-wave power condition.
Should the circuit breaker open (pop-out button will appear) merely depress to reset. If the circuit
breaker continually opens, a failure has occurred in the motor, solid-state speed controller or Airflow
Monitor. Consult a qualified repair technician or NuAire, Inc. for replacement of necessary parts.
6.2 Flow Gard Operation
6.2.1 Overview
The Flow Gard monitor (see Figure 1) uses a digital pressure transducer to monitor the cabinet's
positive pressure plenum. The Flow Gard monitor indicates through LED's normal operation (green),
as well as high alarm status (red) (HEPA filter loading) and low alarm status (red) (low airflow).
The Flow Gard functions only when the cabinet blower is on. When the Flow Gard is turned on, it will
go through a 4-minute warm-up period indicated by a series of blinking LED's. When the warm-up
period is complete, the LED indicate will stop blinking and remain on.
All user interaction is accomplished through the arrow and reset keys. IT IS RECOMMENDED THAT THE
FLOWGARD BE CALIBRATED ANNUALLY DURING THE CERTIFICATION PROCESS.

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6.2.2 Nominal Airflow Calibration
To calibrate the Flow Gard monitor, the cabinet must first be certified or set to nominal airflow values.
Once the cabinet nominal airflows are set, perform the following procedure:
•Press and hold [] and [] arrow keys simultaneously for 3 seconds until the center green LED
blinks. ALLOW UNIT TO RUN FOR A MINIMUM OF 2 MINUTES with the center green LED
blinking.
•Press [RESET] key to enter the nominal airflow value (green LED will stop blinking and display
normal).
Please note, after the initial calibration, the Flow Gard LED display will bounce around more than
normal. After a few hours, the Flow Gard LED display will steady due to fuzzy logic or a learning
program that averages the normal display.
Once the Flow Gard has been calibrated, the high/low alarm limits are defaulted to activate with a
pressure deviation of +0.13 inches (3mm) water gage from nominal. The pressure deviation translates
to a reduction in airflow that is well within the operational tolerances of the cabinet.
6.2.3 Independent High/Low Alarm Calibration
If desired, the Flow Gard high or low alarm limit may be adjusted. However, to adjust the high or low
alarm setpoint, the cabinet airflow must be altered to the desired alarm setpoint before the individual
calibration point can be entered into the monitor. Once the cabinet high or low alarm limit airflows are
set, perform the following procedure:
•Press and hold either the [] key for high alarm or [] key for low alarm for three seconds until
the Hi or Lo red LED blinks respectively. Allow unit to run for a minimum of 2 minutes with the
respective LED blinking.
•Press [RESET] key to enter the high or low alarm setpoint.
•Re-adjust airflow back to the nominal values.
The high or low alarm limit may be verified by adjusting the airflow to the alarm limit to induce an alarm
condition.
6.2.4 Audible Pressure Alarm
The audible alarm should be activated whenever the pressure reaches the high or low alarm setpoint.
However, once the alarm pressure is reached, it must stay on the alarm limit for 5 seconds consistently
or it will not recognize it as an alarm. If at any time, the pressure returns to acceptable limits, the alarm
would be reset and silenced. Once the 5 second period of constant alarm is present, the audible should
sound for 30 seconds, then ringback 1 second every 10 seconds. If the Reset key is pressed, the alarm
should be silenced for 5 minutes, then continue to ringback for 1 second every 10.

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Airflow monitor (instrument panel) displays the status of airflow through the hood. A continuous green light
emitting diode (LED) indicates a satisfactory mass flow (greater than setpoint). When the mass flow being
monitored drops below the setpoint, a yellow light emitting LED is lit. If the low flow condition lasts for greater
than 6 seconds, the red LED begins flashing, the green LED extinguishes and the audible alarm and alarm relay
are enabled. The alarm relay is wired for external connection and may be used to activate a signal of low flow
to a remote location.
Figure 1
Flow Gard Monitor

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6.3 Operating Guidelines
6.3.1 The intent herein is to present general operating guidelines that will aid in the use of the Class I
Containment Cabinet to control airborne contaminants of low to moderate risk.
6.3.2 Procedure protocols defined in terms of the barrier of control concepts unique to containment cabinet
must be developed in order to obtain the maximum potential for safety and protection. The pre-
planning necessary to develop these protocols is based on several fundamental considerations, each of
which will contribute to optimum benefits from the equipment:
a. Minimize disruption of “air curtain”
b. Minimize room activity
6.3.3 The minimum number of items necessary should be placed into the cabinet to prevent overloading, but
the work should also be planned to minimize the number of times an operator’s hands and arms must
enter and leave the air curtain at the open face. The ideal situation is to have everything needed for the
complete procedure placed in the cabinet before starting, so that nothing need pass in or out through
the air barrier at the face until the procedure is completed. This is especially important in working with
moderate risk agents.
NOTE: When working with agents of lower risk, it is not as important for all materials to be
placed in the cabinet before starting, or for the procedure to be completely finished
before materials are removed. Also, the time period for a unit of work may be
continued over a more extended period during which entries and withdrawals from
the hood may be made.
6.3.4 Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air
currents as well as interfere with the work of the operator. A person walking past the front of the
cabinet can cause draft velocities up to 175 fpm, which is sufficient to disrupt the air barrier provided by
the work access opening.
6.3.5 Employ Good Laboratory Practice
The operator must not assume an attitude of “let the cabinet do it” during the procedure with a
containment hood. Properly used, the hood will do an excellent job of containing toxic or noxious
agents. Normal laboratory contamination control techniques are necessary to obtain maximum benefit
from the hood. The good laboratory practices are designed to minimize creating and/or release of
aerosols to the environment and should not be discontinued.
6.3.6 Operating Sequence
Start up -- turn on cabinet blower and lights, check the exhaust grill of the cabinet to make sure it is
unobstructed. Blower speed must only be readjusted by qualified maintenance technicians. Allow
blowers to operate for a minimum of 2 or 3 minutes before manipulations are begun in the cabinet.
6.3.7 Air Purge
Additional purging of the workspace without user activity should be allowed for 2-3 minutes after
materials and apparatus’s have been placed in it. This will rid the area of all “loose” contamination that
may have been introduced with the items.

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6.3.8 Perform Work
The work can now be performed. The technician performing the work is encouraged to wear a long-
sleeved gown with knit cuffs and rubber gloves. This will protect the hands and arms from chemical
contamination. At a minimum, the hands and arms should be washed well with germicidal soap before
and after work.
6.3.9 Terminal Purging and Wipedown
Following completion of the work, allow the hood to run for a 2-3 minute period without personnel
activity to purge the unit. The decontamination of the interior surfaces should be repeated after
removal of all materials. USE OF CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY
DAMAGE STAINLESS STEEL.
6.3.10 Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess laboratory equipment during
periods of non-operation.
CAUTION: If infected animal cages are being cleaned, it is recommended to let the
cabinet run 24 hours per day, seven days a week in order to prevent
contaminants from escaping the filter media during periods of power off
due to room air currents.
6.4 Bag Installation and Removal (See Figure 2)
Attachment of the bag to the underside of the work surface is easily accomplished when the work surface is
raised. Conversely it is easy to also remove the bag for disposal.