NuAire AllerGard NU-620-300 Manual

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AllerGard™ ES Energy Saver Containment
Small Animal Transfer Station
Models
NU-620-300/400/500
NU-620-301/401/501
Operation & Maintenance Manual
June, 2019
Revision 5
Series 5
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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About this Operation and Maintenance Manual
The information contained in this manual is intended to reflect our current production model along
with the more frequently purchased options. Any unique additions, modifications, or shop drawings are
appended in the back flap of this manual, along with any modifications and/or additions to procedures as
outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this
manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be
obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description
of the information desired.

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AllerGard™ ES Energy Saver Containment
Small Animal Transfer Station
Models
NU-620-300/400/500
NU-620-301/401/501
Operation & Maintenance Manual
Table of Contents
Section No. 1 ..............................................................................General Description
Section No. 2 ..............................................................................Models & Features
Section No. 3 ..............................................................................Warranty
Section No. 4 ..............................................................................Shipments
Section No. 5 ..............................................................................Installation Instructions
Section No. 6 ..............................................................................Operating the NU-620
Section No. 7 ..............................................................................Operating Guidelines
Section No. 8 ..............................................................................General Maintenance
8.1…………………………………………. ..........................................Preventative Maintenance
8.2 .......................................................................................LED Lamp Replacement
8.3 .......................................................................................HEPA Filter Replacement
8.4 .......................................................................................Motor Blower Replacement
8.5 .......................................................................................Airflow Control System Setup
8.6 .......................................................................................Airflow Calibration
8.7.......................................................................................Filter Integrity Check
8.8.......................................................................................PresurFlow™ Alarm Set Points
8.9.......................................................................................Decontamination
Section No. 9 ..............................................................................Polycarbonate Material Compatibility
Section No. 10 ............................................................................Error Indicators, Troubleshooting & Option
Parameters
Section No. 11 ............................................................................Remote Contacts
Section No. 12 ............................................................................Electrical/Environmental Requirements
Section No. 13 ............................................................................Disposal and Recycle
Section No. 14………………………….................................................PM Checklist
Manual Drawings
BCD-16818 .................................................................................620-30X Specification Drawing
BCD-16819 .................................................................................620-40X Specification Drawing
BCD-16820 .................................................................................620-50X Specification Drawing
BCD-16521 .................................................................................Front Panel
Electrical Schematic
BCD-16821 .................................................................................620-30X/40X/50X Electrical Schematic

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AllerGard™ ES Energy Saver Containment
Small Animal Transfer Station
Models
NU-620-300/400/500
NU-620-301/401/501
MANUFACTURED BY:
NuAire, Inc. - Plymouth, Minnesota, USA
1.0 General Description
The NuAire AllerGard™ ES Energy Saver Containment Small Animal Transfer Station Model NU-620 is a station whose
unique configuration offers the benefits of vertical HEPA filtered airflow for product protection on the worksurface. In
addition, the NU-620 provides improved allergen personnel protection by incorporating a HEPA filtered down draft vacuum
on the worksurface to assure minimal exposure to animal hair/dander as well as cage bedding material. However, the
NuAire Model NU-620 should not be used as a substitute for a Class II, Type A2 Biosafety Cabinet (BSC). The NU-620 is
not a containment device for airborne particulate in low to moderate risk-hazard research as prescribed by the CDC/NIH
Biosafety in Microbiological and Biomedical Laboratories.
The NU-620 design and basic airflow pattern is illustrated on the specification drawing. The NU-620 design incorporates
two impeller/blowers, both a supply and exhaust, each with its own associated HEPA filter providing excellent system
function and maximum filter loading capacity. The designed airflow pattern, being a single pass system, also eliminates any
heat buildup within the workzone. The airflow pattern starts with the supply prefilter located on top of the station. The
airflow continues through the supply impeller/blower and forced into the supply HEPA filter providing 99.99% particle
removal at 0.3 micron. The airflow continues to move in a uniform vertical downflow pattern providing particulate-free air
in the workzone.
NuAire offers two window configurations being either dual access or single access. The dual access design along with the
work surface configuration allows the greatest access while maintaining work zone sterility and improved allergen
protection. The single access design meets BSC containment performance levels for personnel and protection.
The downdraft HEPA filter provides 99.99% particle removal at 0.3 micron and is located under the worktray in its own
impeller/blower/filter cabinet. As the air is pulled from the worktray, through the prefilter and into the blower, the air then
passes through the downdraft HEPA filter and is exhausted back to the room providing improved allergen personnel and
environmental protection.

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1.1 Safety Instructions
These safety instructions describe the safety features of the AllerGard™ ES Energy Saver Small Animal Transfer
Station Model NU-620. The station has been manufactured using the latest technological developments and has
been thoroughly tested before delivery. However, the station may present potential hazards if it is not installed
and used as instructed for its intended purpose or outside of operating parameters. Therefore, the following
procedures must always be observed:
•The station must be operated only by trained and authorized personnel.
•For any operation of this unit, the operator must prepare clear and concise written instructions for
operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical
regulations, in particular.
oWhich decontamination measures are to be applied for the station and accessories?
oWhich protective measures apply while specific agents are used?
oWhich measures are to be taken in the case of an accident?
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the station so that safety instructions and important information
are always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please
contact your NuAire Representative of NuAire technical Services.
1.2 Explanation of Symbols
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death of
serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property damage.
Potential electrical hazard, only qualified person to access.
Note: Used for important information.
Biohazard
Ground, Earth
CAUTION

Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Lead Free

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2.0 Models & Features
The Model NU-620 AllerGard™ ES Energy Saver Containment Small Animal Transfer Station
is manufactured in 3 sizes:
Dual Sided
Single Sided
620-300
3 ft. (.91m)
620-301
3 ft. (.91m)
620-400
4 ft. (1.2m)
620-401
4 ft. (1.2m)
620-500
5 ft. (1.5m)
620-501
5 ft. (1.5m)

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3.0 Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
Within the data packet sent with the unit.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your ALLERGARD™ ES ENERGY SAVER CONTAINMENT SMALL
ANIMAL TRANSFER STATION arrives without damage. Motor carriers are carefully selected and shipping cartons have been
specially designed to insure your purchase. However, damage can occur in any shipment and the following outlines the
steps you should take on receipt to be sure that if damage has occurred, the proper claims and actions are taken
immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.

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5.0 Installation Instructions
5.1 Location
The NU-620 Station can be located anywhere within the animal facility. However, for maximum product protection, the
station should be used away from laboratory personnel traffic lanes, air vents (in or out), doors, and/or any other source of
disruptive air currents.
If drafts or other disruptive air currents exceed the downflow velocity of the station through the access opening, the
potential exists for contaminated room air to enter the work zone. Remember: Always use proper laboratory techniques.
5.2 Set-Up Instructions
Remove outer shipping protection (carton or crating). The station is fastened to the base skid and it is usually the best
procedure to leave the skid in place until the station is located in its approximate position to facilitate ease in handling. It
can be disconnected from the skid by removing the brackets, bolts, and screws holding the station to the skid, then remove
the screws to disconnect the ends of the skid.
The station has two on-site items required for the adjustable base to function.
After the cable ties have been removed and the power cable is plugged in for the unit. The NU-620 can be raised onto its lift
system and rolled away from the end of the skid. It will take 5-10 seconds of holding the UP bottom for the lift to begin to
move for the first time.
5.3 Plumbing Services
Typically, the NU-620 is not provided with plumbing services. In special cases, ground key cocks, needle valve cocks, or
customer specified watering system hardware can be added. If ground key cocks are added, the type of service would be
specified by the removable button on the handle. The Ground Key cocks are not recommended for pressure over 30 psi
(2.0 BAR). Reducing valves should be installed external to the station if necessary. Ground key cocks should never be used
for oxygen service. A special needle for oxygen service is required and available upon request.
External connection is to 3/8 inch NPT coupling in the inner sidewalls. Plugged clearance holes in the outer sidewalls are
provided for easy access to the inner couplings via a NPT nipple. Connection to plant utilities should be made with proper
materials for the individual service and according to national and/or local codes. It is not recommended that flammable
gases be used in the station. However, if flammable gas is used, emergency shut-off valves should be located in an
accessible area external to the station. Observe all labels pertaining to the type of service and operating pressure.
THIS UNIT HAS NOT BEEN EVALUATED FOR USE WITH
FLAMMABLE, TOXIC OR EXPLOSIVE SUBSTANCES.
USE AT YOUR OWN RISK.
CAUTION
!

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5.4 Electrical Services
The NU-620 is provided with an electrical power cord. The station requires 115 VAC, single phase power and is
recommended to be on its own branch circuit, protected with a 15 Amp circuit breaker at the distribution panel.
NOTE: THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT/LED LIGHTING. ELECTRONIC BALLASTS
OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT
INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO TRIP.
5.5 Certification
After installation and prior to use, NuAire recommends that the station be recertified to factory standards. At a minimum,
the following tests should be performed (see Section 8.5).
1. HEPA Filter Integrity/Housing & Frame Leak Test
2. Downflow Air Velocity Measurement
3. Exhaust Motor/Blower Setup
It is recommended that these tests be performed by a qualified technician who is familiar with the methods and procedures
for certifying Biosafety Cabinets.
After the initial certification, NuAire recommends that the station be recertified at a minimum on an annual basis and after
every filter change or maintenance action or any time the operator feels it is necessary. Note that the station's filters and
seals provide premium performance; Quality Control in both design and manufacturing assure superior reliability.
However, protection to the work in process can be so vital that certification to the performance requirements should be
accomplished as stated to insure safety established by the factory standards.


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AllerGard™ ES Energy Saver
Containment Small Animal Transfer Station
Models
NU-620-300/400/500
NU-620-301/401/501
Catalog Number
Catalog Number
NU-620-300
NU-620-301
Nominal 3 foot (0.9m)
NU-620-400
NU-620-401
Nominal 4 foot (1.2m)
NU-620-500
NU-620-501
Nominal 5 foot (1.5m)
Performance Specifications
NU-620-XX0 Product / Personnel
NU-620-XX1 Product / Personnel
ISO 4 / Improved Allergen
ISO 4 / NSF/ANSI 49
ISO 4 / Improved Allergen
ISO 4 / NSF/ANSI 49
ISO 4 / Improved Allergen
ISO 4 / NSF/ANSI 49
Diffuser for Air Supply (Metal) Non-flammable Non-flammable Non-flammable
Supply Filter-99.99% Eff. on 0.3 microns
Exhaust Filter-99.99% Eff. on 0.3 microns Neoprene, Spring-loaded Neoprene, Spring-loaded Neoprene, Spring-loaded
Fumigation : per NIH/NSF Procedures Yes Yes Yes
Standard Services:
Duplex Outlet One Sidewall One Sidewall One Sidewall
Optional Services:
Gas Cocks 3/8" NPT Up to 3 ea. Sidewall Up to 3 ea. Sidewall Up to 3 ea. Sidewall
Optional Services:
Folding Shelf (Either Side) 14 (355) X 30 (762) 14 (355) X 30 (762) 14 (355) X 30 (762)
Cabinet Size Inches (mm):
Height (Fully Assembled, minimum)
Height (Fully Assembled, maximum)
Height (Minimum for Transport without
Castors)
Width (with handles)
Depth
80 ½ (2045)
92 ½ (2350)
75 (1905)
48 (1220)
30 5/8 (777)
80 ½ (2045)
92 ½ (2350)
75 (1905)
54 (1372)
30 5/8 (777)
80 ½ (2045)
92 ½ (2350)
75 (1905)
66 (1677)
30 5/8 (777)
Work Access Opening Inches (mm):
Standard Opening Height 14 (356) 14 (356) 14 (356)
Work Zone Inches (mm):
Height
Width
Depth
25 ½ (648)
36 5/8 (929)
28 ¼ (718)
25 ½ (648)
42 5/8 (1081)
28 ¼ (718)
25 ½ (648)
54 5/8 (1387)
28 ¼ (718)
Viewing Window Inches (mm): Polycarbonate Polycarbonate Polycarbonate
Heat Rejected, BTU, Per Hour 785 785 785
Electrical:
Volts, AC 60 Hz
+Amps: Blower/Lights
Amps: Duplex
Rated Amps:
25 ft. (7.62m) Power Cord
U.L./U.L.-C Listed
115
5.0
3
12
Cord Reel-15A
U.L./U.L.-C Listed
115
5.5
3
12
Cord Reel-15A
U.L./U.L.-C Listed
115
6.0
3
12
Cord Reel-15A
Crated Shipping Weight:***
Net Weight
480 lbs. /218 kg.
430 lbs. /195 kg.
510 lbs. /231 kg.
460 lbs. /209 kg.
580 lbs. /263 kg.
530 lbs. /240 kg.
***Crated shipping weight does not include weight for accessories or options
+ Based on cabinet with new filters running at 115VAC.

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6.0 Operating the NU-620
6.1 Aeromax™ Control System
6.1.1 Overview
The Aeromax™ control system is designed to service the control requirements of the NU-620. The Aeromax™
control system consists of an electronic module that will perform the following functions:
•Easy user interface via LED’s and function keys
•Control blower via solid state switch.
•Control lights via solid state switch.
•Control outlets via solid state switch.
•Disable audible alarm switch with ring back function.
•Control blower DC ECM motor with solid-state DC Motor Controller that provides automatic
compensation for line voltage variances.
•Monitor and display airflow system performance via PresurFlow™ monitor.
The NU-620 offers the latest digital microprocessor design technology for improved cabinet performance and
safety. The Aeromax™ control system integrates a digital pressure sensor (PresurFlow™) to monitor the cabinet’s
airflow performance. The Aeromax™ control system also integrates a DC motor controller that provides automatic
compensation for line voltage variances. There is additional on/off control of blower, fluorescent/LED light,
ultraviolet light (optional) and outlets. All the above functions are shown in a system block diagram (see figure 1).
Figure 1

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6.1.2 Front Panel
The control system front panel contains the following functions described in detail (see Drawing BCD-16521).
6.1.2.1 Blower Keys
The blower key controls the ON/OFF power to the blower.
LED above key indicates: full green for blower on,
blinking green for blower pending and
full red for blower alarm.
6.1.2.2 Hidden Key
The hidden key is located just above the blower LED indicator centered in the airflow symbol. The hidden key
is used for various functions including the blower password 3 key sequence if the option is activated.
6.1.2.3 LED Light Key
The LED light key controls the on/off power to the LED light.
LED above the key indicates full blue for fluorescent light on.
6.1.2.4 Ultraviolet (UV) Light Key (Typically Not Used on this model)
6.1.2.5 Outlet Keys
The outlet key controls the ON/OFF power to the outlet.
LED above indicates full blue for outlets on.
6.1.2.6 Red Alarm LED
The red alarm LED will indicate any alarm condition and remain indicating until the alarm condition is cleared.
6.1.2.7 Audible Alarm Silence
The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15 minutes.
After 15 minutes if the alarm condition still exists, the audible alarm will again sound.
The audible alarm silence key also is used to exit all Aeromax™ user interaction menus.
6.1.2.8 Arrow Adjustment Keys
The arrow adjustment keys allow user interaction for various functions.

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6.1.3 Aeromax™ Control System Power
After the NU-620 is plugged into the appropriate facility line power the control system will power up. The control
panel will also indicate the power up status by blinking the red alarm LED. Pressing any key will acknowledge the
power up status and turn off the blinking red alarm LED.
If a power interruption occurs, all control system functions, calibrations and parameters will be maintained and
continue upon restoration of power. Just as the initial power up, the red alarm LED will blink to indicate power up
status.
6.1.4 Standby Mode
When the ATS is not in use any of the function keys except the blower that initiates run mode may be turned on
and off in standby mode.
6.1.5 Run Mode
Any time the blower run key is pressed the RUN MODE screen will be initiated. The Run Mode will start with the
PresurFlow™ entering and approximate 3-minute warm up period. The PresurFlow™ LED indicators will blink and
indicate the following sequence:
•1st minute – Left and right Red LED’s will blink
•2nd minute – Left and right Green LED’s will blink
•3rd minute – Center 3 Green LED’s will blink
Once the warm up period is complete, only one LED will indicate cabinet airflow status.
During the warm up period the aseptic cleaning process may begin.
6.1.6 Standby/Run Mode Alarms
If present, standby/run mode alarms will be both visual and audible, the red alarm LED oval will turn on. Audible
alarms will produce an alarm tone for 30 seconds, then ring back for 2 seconds of every 5 seconds. Pressing the
alarm silence key will silence the audible alarm for 15 minutes initially then will start the ring back function again.
The list below represents alarm types and their respective priority from the highest to lowest priority.
1) New Firmware Loaded
2) Internal Board Failure
3) Power on Reset
4) Airflow Pressure Alarm
5) Blower RPM Failure
Note: The above messages are described in greater detail in section 10.0.


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6.1.8 Operator Accessible Functions
6.1.8.1 Access and Navigation
To access the operator accessible functions,
•Press and hold the key, then enter the 3-key sequence for the desired function, then release the
key and follow each instruction set.
Note: Pressing the key at any time will abort and exit the process without saving any changes
made. Pressing the hidden key will accept all changes and exit.
6.1.8.2 Auto Timer Duration
Auto timer duration timers are countdown timers for the functions displayed once time is entered into a
function. The timer will begin to countdown upon the start of that function (i.e. press UV light key to start
timing the UV light). The LED indicator above the function key will start to blink indicating the timer function.
If the LED indicator was full on, no timer function is present. As the timer expires the function will turn off.
•Select auto timer duration function
oOutlets
Press and hold key, then press hidden – outlet – outlet keys sequentially.
LED indicator above outlet will blink fast. Adjust desired time as described below.
oLights
Press and hold key, then press hidden – light – light keys sequentially.
LED indicator above light will blink fast. Adjust desired time as described below.
oAdjust countdown time
Press ↑ or ↓ keys to adjust time.
Time will change in 15-minute increments as shown on the PresurFlow™ LED segments
below.
15 minutes for Red LED 30 Minutes for Yellow LED
1 hour for green LED Represents 8 hours (maximum time)
•Press hidden key to accept time and exit.
•Press key at any time to abort and exit.


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6.1.8.3 Blower Password
The blower on/off password allows the cabinet user to place a 3 key sequence requirement to turn the blower
on or off.
The 3-key sequence for the blower password will be a combination of the hidden and blower keys.
•Select blower password
Press and hold key, then press hidden – blower – hidden keys sequentially.
Red LED indicator above blower will blink fast.
•Select password
Press ↑ or ↓ key to scroll through the code choices below,
No Password
B-B-H password
B-H-B password (default)
B-H-H password only
H-B-B password
H-B-H password
H-H-B password
•Press hidden key to accept time and exit.
•Press key at any time to abort and exit.
Note: If the required blower password option is selected in the blower airflow option menu (see section 8.2.2).
Then the “No password” choice above is not available and the default remains B-H-B.


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7.0 Operating Guidelines
It is acceptable and sometimes recommended to operate the station continuously. The station will then remain in its
initially clean condition. If for any reason the unit is turned off for some time, daily or weekly, turn the station on and run
for 10 minutes before using.
Allow only essential items in the work station. New items introduced into the work area should be placed within one of the
worksurface quadrants to minimize cross contamination. All work should be performed with the operator's hand
downstream of the critical process points. Unnecessary movement with the work station should be kept to a minimum.
7.1 Operating Sequence:
Start-Up
Turn on station's blower and lights. Blower speed must only be readjusted by qualified maintenance technicians. Good
procedure includes the decontamination or wipe down of station surfaces with chemical disinfectant before work
commences.
Note: See cleaning procedures section 7.3
Allow blower to operate for a minimum of 10 minutes before aseptic manipulations are begun in the station. An additional
advantage is obtained from purification (filtration) of the room air circulated through the equipment. Because of the
characteristics contributed to the quality of the laboratory environment, some owners leave them in operation beyond the
time of actual use.
Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air currents as well as
interfere with the work of the operator. A person walking past the front of the station can cause draft velocities up to
175fpm (.88 m/s) which are sufficient to disrupt the air balance of the Laminar Flow Unit.
Utilize Unidirectional Airflow
The operator must keep two important facts in mind:
1. The air as supplied to the work area through the HEPA filter is contaminant free.
2. Airborne contamination generated in the work area is controlled by the unidirectional vertical flow air stream.
A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of
controlling lateral movement of airborne particulates. A cone of turbulence extends behind the object and
laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three to
six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally by
multidirectional eddy currents. The downdraft vacuum source on the worksurface greatly reduces the above
effect. However, working with proper technique is always the best method.
Terminal Purging & Wipe down
Following completion of the work, allow the station for a 2-3 minute period without personnel activity to purge the
unit. The decontamination of the interior surfaces should be repeated after removal of all work materials.
Prefilters
Located under the work surface is a prefilter area. This area forms the air path to the exhaust motor/blower. If the
air flow is blocked by excessive animal dander or bedding materials, the performance of the station could be
seriously affected. Therefore, THE PREFILTERS SHOULD BE CHECKED AND CLEANED NO LESS THAN ON A WEEKLY
BASIS. The prefilters may be vacuumed to remove the materials. The prefilters may also be replaced as necessary
(see replacement parts list).
Shut Down
Turn off blowers and lights. Do not use station as a depository for excess laboratory equipment during periods of
non-operation.
