NuAire NU-C300V Manual

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NuAire, Inc. |2100 Fernbrook Lane |Plymouth, MN 55447 |U.S.A |ph: 763.553.1270 | fx: 763.553.0459 |tf: 800.328.3352 |www.nuaire.com
NUWIND CENTRIFUGE
Models
NU-C300V
NU-C300R
NU-C300V-E
NU-C300R-E
NU-C300RF
NU-C300RF-E
Operation & Maintenance Manual
May, 2020
Revision 4
(120 VAC, 60 Hz) (230 VAC, 50 Hz)
Manufactured by:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, Minnesota USA 55447
Toll Free: 1-800-328-3352
In MN: 763-553-1270
Fax: [phone]

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
You have just purchased one of the finest Laboratory Centrifuges available. With proper care,
maintenance and laboratory procedure, this centrifuge will provide you years of productive service. Please
read this manual carefully to familiarize you with proper installation, maintenance and operation of the
centrifuge. Other reference and guideline materials are available on our website, www.nuaire.com.

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
NUWIND Centrifuge
TABLE OF CONTENTS
Section No. 1 ..........................................................General Information
Section No. 2 ..........................................................Models Specifications and Accessories
Section No. 3 ..........................................................Warranty
Section No. 4 ..........................................................Shipments
Section No. 5 ..........................................................Installation Instructions
5.1 ............................................................................ Location
5.2 ............................................................................ Set-up Instructions
5.3 ............................................................................ Certification Testing Methods and Equipment
Section No. 6 ..........................................................Operating the NU-C300
6.1 ............................................................................ First Start / Powering
6.2 ............................................................................ Operation / Interaction
Section No. 7 ..........................................................General Maintenance
Section No. 8 ..........................................................Error Messages, Troubleshooting, Option-Diagnostics & Performance
Verification
Section No. 9 ..........................................................Electrical/Environmental Requirements
Section No. 10 ........................................................Disposal and Recycle
Compliance Certificate ...........................................Declaration of Conformity
Annex 1.0 ...............................................................Theoretical Basics of Centrifugation
Annex 2.0 ...............................................................Accessary Specifications
ELECTRICAL SCHEMATICS
BCD-18882 ......................................NU-C300V / NU-C300V-E
BCD-18883 ......................................NU-C300R / NU-C300R-E

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
NUWIND Centrifuge
1.0 General Information
1.1 Description
The NUWIND 3.0 is a laboratory benchtop series of centrifuges that maximizes productivity while minimizing laboratory
required bench space. The centrifuge functionality allows the separation of substances of different densities held in
suspension or emulsion in a liquid using centrifugal force.
The NUWIND 3.0 centrifuge is available in two models, one ventilated and the other refrigerated.
The NU-C300V ventilated model has a unique ventilation system to maximize performance within laboratory ambient
temperature conditions.
The NU-C300R and NU-C300RF refrigerated model adds precise temperature control to further maximize performance.
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.
Centrifuges NUWIND 3.0: ventilated and refrigerated models
Intended use
The centrifuge is designed and manufactured for use with rotating accessories supplied by NuAire, Inc. It is suitable for
separating substances of different densities held in suspension, or emulsion in a liquid.
The maximum density of substances should be 1.2 g/ml.
The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may
threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment.
Users of the equipment must be trained on good centrifugation practices, as well specific usage of the NUWIND models and
Accessories. Any use outside the framework defined above is considered non-compliant.
NuAire, Inc. assumes no responsibility for injury due to improper use.
CAUTION
!

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
1.2 Safety Instructions
The operator must observe the following precautions when using the centrifuge:
•Ensure stability and perfect levelling of the centrifuge.
•Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.
•Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and
mechanical strength at the centrifugal force applied thereto.
•Check the condition of the tubes and remove damaged tubes.
•Use only the rotors and accessories authorized by the manufacturer NuAire, Inc.
•Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.
•Maintain and control accessories.
•Balance the load of the rotor around the axis of rotation.
•Respect the maximum density of 1.2 g / ml, in particular in case of a cycle at full speed.
•Limit volume in case of excess density.
•Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the
centrifuge.
•During operation, the centrifuge must not be moved or be subject to impact.
•Do not attempt to open the lid while the rotor is spinning.
•Do not attempt to neutralize the motorized lid lock
•Do not lean over the centrifuge during the spin cycle.
•Do not stay within a space of 10 inch around the centrifuge longer than necessary.
•Do not leave potentially dangerous materials inside the free space.
•Use aerosol-barrier accessories when centrifuging bio hazardous material.
•Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment.
Allow the centrifuge to warm up for two hours before use.
Prohibitions:
The centrifuge is not designed for the following uses and environments.
The following are prohibited:
•Flammable, explosive, toxic and radioactive materials
•Materials which may react, causing a hazard
•Contaminated materials not contained in an aerosol-barrier container
•Rotating rotors and accessories that have exceeded their maximum use.
•Rotating rotors and accessories that are not maintained according to the instructions in this manual,
or showing signs of wear and/or corrosion.
•Rotating rotors and accessories not supplied by NuAire, Inc.
•Explosive environments
•Radioactive environments
Only operate a centrifuge after taking all necessary safety measures.
Rotors and buckets should be removed from use in case of mechanical fault
trace, or corrosion. These elements have a life cycle duration, engraved on
their visible part: To maintain safe conditions, it is imperative to replace them
when the recommended duration is reached.
CAUTION
!

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
1.3 Explanation of Symbols
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, could result in death or serious injury.
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property
damage.
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, may result in minor or moderate injury.
Pinching: This warning symbol indicates the presence of a risk of
pinching when handling the lid.
Note: Used for important information
Potential electrical hazard, only qualified person to access
Biohazard
Ground, Earth
Lead Free
CAUTION
!
CAUTION
WARNING
!


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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
2.0 Models, Specifications and Accessories
2.1 Specifications
Description
Laboratory Centrifuge
Environmental Conditions
For indoor use only
Environment Temperature Range: : 60⁰F - 85⁰F (15⁰C - 30⁰C)
Environment Humidity: Maximum relative humidity 80% for
temperatures up to 31⁰C decreasing
linearly to 50% relative humidity at 40⁰C
Environment Altitude: 6562 Feet (2000 meters) above sea level
Maximum capacity
4x750 ml x kg / bucket, 4 x buckets
Models
Ventilated
Refrigerated
Refrigerated Floor
Catalog Number
NU- C300V
NU-C300V-E
NU-C300R
NU-C300R-E
NU-C300RF
NU-C300RF-E
Supply Voltage
(Depending on the model)
120 V ±10%
60 Hz
230 V ±10%
50Hz
120 V ±10%
60 Hz
230 V ±10%
50Hz
120 V ±10%
60 Hz
230 V ±10%
50Hz
Nominal intensity (A)
9 A
4 A
17 A
9 A
17 A
9 A
Maximum Speed (rpm)
10,700 rpm NU-RA8-50
(Depending upon the rotor)
12,100 rpm NU-RA8-50
(Depending upon the rotor)
12,100 rpm NU-RA8-50
(Depending upon the rotor)
Maximum Relative
Centrifugal Force (g)
13,000 xg 16,660 xg 16,660 xg
Total Power Consumed
1000 Watt
1700 Watt
1700 Watt
Dissipated Heat (BTU/h)
(1 BTU/h = 0.29307 W)
3413 BTU/h 5801 BTU/h 5801 BTU/h
Fluid Coolant / Charge
Not applicable
R404A / 0.35 kg
R404A / 0.35 kg
Ambient Operating
Temperature
20⁰C ± 2⁰C 20⁰C ± 2⁰C 20⁰C ± 2⁰C
Temperature adjustment range
N/A
-10 ° C to 40 ° C
-10 ° C to 40 ° C
Temperature control precision
N/A
± 2 ° C
± 2 ° C
Dimensions on bench
(H x W x D)
37 x 50 x 66 cm
14.6 x 19.7 x 26 inches
35 x 77 x 68 cm
13.8 x 30.3 x 26.8 inches
83 x 67 x 69 cm
32.7 x 26.4 x 27.2 inches
Net weight (excluding rotor)
82 kg /180 lbs.
145 kg / 320lbs.
170 kg / 375lbs.
Packing (H x W x D) / Weight
54 x 74 x 77 cm
21.3 x 29.1 x 30.3 / inches
97 kg / 215 lbs.
54 x 90 x 80 cm
21.3 x 35.4 x 31.5 inches
160kg / 350 lbs.
100 x 80 x 80 cm
39.4 x 31.5 x 31.5 inches
185kg / 410 lbs.
Maximum sound level
≤61 dB(A)
≤60 dB(A)
≤57 dB(A)
Integrated systems
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Control System
Microprocessor
Microprocessor
Microprocessor
Acceleration slopes
9
9
9
Braking slopes
10
10
10
Speed control precision
± 10 rpm
± 10 rpm
± 10 rpm
Number of programs
99
99 + Pre-cooling + Pre-warming
99 + Pre-cooling + Pre-warming
Optimal settings refrigeration
N/A
+4 ° C temperature with
NU-RX750 rotor can be
maintained at 3,600RPM at an
ambient temperature of
20⁰C ± 2⁰C.
+4 ° C temperature with
NU-RX750 rotor can be
maintained at 3,600RPM at an
ambient temperature of
20⁰C ± 2⁰C.

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
2.2 Accessories
References
Description
Lot
NU-RX1000
Swing-Out rotor 4x 1000 ml
One Unit
NU-B1000
1000 ml buckets
Set of 4
NU-L1000
Lid 1000 ml
Set of 4
NU-BXDW4
Microplates carriers
Set of 2 or 4
NU-4T2
1,5 - 2ml MicroTube
Set of 2 or 4
NU-4T5
Tube insert 50x 5 ml
Set of 2 or 4
NU-4T10
Tube insert 39x 10 ml
Set of 2 or 4
NU-4T15C
Conical tube insert 24 x 15 ml
Set of 2 or 4
NU-4T50C
Conical tube insert 10 x 50 ml
Set of 2 or 4
NU-2T200C
2 x 175/225* ml Conical Bottle
Set of 2 or 4
NU-4T250C
2 x 250* ml Conical Bottle
Set of 2 or 4
NU-4T290F
2 x 290* ml Flat Bottle
Set of 2 or 4
NU-4T500C
1 x 500* ml conical bottle
Set of 2 or 4
NU-4T265
1 x 625 ml Flat Bottle
Set of 2 or 4
NU-4T750
1 x 750 ml Flat Bottle
Set of 2 or 4
NU-RX750
Swing-Out rotor 4x 750 ml (3 liters)
One unit
NU-B750
750 ml buckets
Set of 4
NU-L750
Lid 750 ml
Set of 4
NU-3T2
1,5 - 2ml MicroTube
Set of 2 or 4
NU-3T5
Tube insert 27x 5 ml
Set of 2 or 4
NU-3T10
Tube insert 21x 10 ml
Set of 2 or 4
NU-3T15C
Conical tube insert 14 x 15 ml
Set of 2 or 4
NU-3T50CL
Conical tube insert 6 x 50 ml
Set of 2 or 4
NU-3T50C*
Conical tube insert 7 x 50 ml
Set of 2 or 4
NU-3T50S
Self-standing conical tube insert 5 x 50 ml
Set of 2 or 4
NU-3T200C
175/225* ml Conical Bottle
Set of 2 or 4
NU-3T500C
500* ml Conical Bottle
Set of 2 or 4
NU-3T750
Flat bottom bottle 1x 750mL
Set of 2 or 4
NU-BXDW
Microplate Bucket X
Set of 4
NU-RHDW
Microplate Rotor H
One unit
NU-BHDW
Microplate Bucket H
Set of 2
NU-RA6-100
6x 100 ml 28° angle rotor
One unit
NU-RA8-50
8x 50 ml 25° angle rotor
One unit
NU-RA16-5
16x 5 ml 45° angle rotor
One unit
NU-RA48-2
48x 2 ml 45° angle rotor
One unit
NU-RA30-2
30x 2 ml 45° angle rotor
One unit
(*) these inserts cannot be used with Bio-containment lids

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
3.0 Warranty
NuAire, Inc. warrants NU-C300 Series NUWIND Centrifuge that it will repair F.O.B. its factory or furnish without charge
F.O.B. its factory a similar part to replace any material in its equipment within 60 months after the date of sale if proved to
the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall
be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories
are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion
of the equipment modified by the user. Claims under this warranty should be directed to NuAire, Inc. setting forth in detail
the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident,
shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this
product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any
product shipped to NuAire without this number will be returned refused shipment or collect freight.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your NUWIND Centrifuge arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a NuAire NUWIND Centrifuge to be
sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
5.0 Installation Instructions
5.1 Handling and transport
The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.).
Please ensure that the people in charge of handling are qualified to handle the lifting equipment.
Maintain the following conditions during transport:
•Temperature: -20 to 50 ° C
•Relative humidity: <90%, under the dew point
5.2 Unpacking
Remove the cardboard packaging made of 2 parts (a lid and a bottom).
The NUWIND centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the
centrifuge, and its spinning accessories, please make sure you are in possession of the following:
5.2.1 Packing list
1) or
2)
or
3)
NUWIND 3.0 Centrifuge Model
1) Ventilated Benchtop or
2) Refrigerated Benchtop or
3) Refrigerated Floor Standing
Compliance Certificate User Manual (Flash Drive)
or
Power Cord
Manual release key
Food grade grease
CAUTION
!
NUWIND
Centrifuge
Operation and
Maintenance
Manual
Declaration
Of
Conformity