NuAire Labgard NU-427-400 Manual

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Labgard ES Energy Saver Class II,
Type B1 Laminar Flow
Biosafety Cabinet
Models
NU-427-400/600
Bench/Console
Operation & Maintenance Manual
NU-427-400/600 Series BR
Revision 2 September 2020
Manufactured By:
Nuaire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]
Congratulations!
You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care, maintenance
(certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate

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contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize yourself with proper installation,
maintenance and operation of the cabinet. Other reference and guideline materials are available through the following web sites.
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa-canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.osha.gov/dts/osta/
www.nuaire.com

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ABOUT THIS OPERATION & MAINTENANCE MANUAL
The information contained in this manual is intended to reflect our current production standard configuration model along with
the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back flap of this
manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test
report is also appended to this manual. In case this manual and/or test report is lost or misplaced, Nuaire retains a copy on file. A
replacement copy can be obtained by calling or writing Nuaire, Inc. stating the model number and serial number and a brief
description of the information desired.

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Labgard ES Energy Saver Class II, Type B1
Laminar Flow Biosafety Cabinet
Models NU-427-400/600 Operation & Maintenance Manual
TABLE OF CONTENTS
Section No. 1.......................................................General Information
Section No. 2.......................................................Models & Features
Section No. 3.......................................................Warranty
Section No. 4.......................................................Shipments
Section No. 5.......................................................Installation Instructions
5.1 ............................................................ Location
5.2 ............................................................ Set-up Instructions
5.3 ............................................................ Exhaust Air Checks
5.4 ............................................................ Certification Testing Methods and Equipment
Section No. 6.......................................................Operating the NU-427
6.1 ............................................................ Biosafety Cabinet Control
6.2 ............................................................ Operating Guidelines
6.3 ............................................................ Operating Sequence
6.4 ............................................................ Ergonomics
6.5 ............................................................ Cleaning Procedures
6.6 ............................................................ Hazardous Drug Decontamination Procedure
Section No. 7.......................................................General Maintenance
7.1 ............................................................ Decontamination
7.2 ............................................................ LED Lamp Replacement
7.3 ............................................................ HEPA Filter/Motor Replacement
7.4 ............................................................ Sliding Window Replacement & Adjustment
7.5 ............................................................ Airflow Control System Setup & Calibration
7.6 ............................................................ HEPA Filter Leak Test
7.7 ............................................................ Airflow Smoke Pattern Test
7.8 ............................................................ Site Installation Assessment Tests
7.9 ............................................................ Cleanliness Classification Test for Pharmacy Application
7.10 .......................................................... Main Control Board Description & Replacement
7.11 .......................................................... Digital Airflow Sensor Description & Replacement
Section No. 8.......................................................Error Indicators, Troubleshooting, Option-Diagnostics &
Airflow Sensor Performance Verification
Section No. 9.......................................................Remote Contacts
Section No. 10.....................................................Optional Equipment
10.1 ........................................................... Ultraviolet Light
Section No. 11.....................................................Electrical/Environmental Requirements
Section No. 12.....................................................Disposal and Recycle
MANUAL DRAWINGS
ACD-12334.................................................NU-427 Airflow Schematic
BCD-14637.................................................NU-427-400 Specification Drawing
BCD-14638.................................................NU-427-600 Specification Drawing
BCD-11815.................................................Drain Valve Installation
BCD-05572.................................................Butterfly Valve Installation
BCD-05660.................................................Bag-In/Bag-Out Procedure
BCD-05659.................................................Base Cabinet Assembly
ASSEMBLY DRAWINGS
BCD-05147.................................................Base Stand Assembly
BCD-05146.................................................Base Stand Storage Cabinet Assembly
BCD-11817.................................................Control Center & Front Decorative Panel Assembly
BCD-11818.................................................Sliding Window Assembly & Adjustment
BCD-14185.................................................Base Cabinet Assembly
ELECTRICAL SCHEMATICS
CD-000055 (Sheets 1-3) ............................NU-427-400/600 Electrical Schematic

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Labgard ES Energy Saver Class II, Type B1
Laminar Flow Biosafety Cabinet
Models
NU-427-400/600
MANUFACTURED BY:
Nuaire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LABGARD ES Model NU-427 Laminar Flow Biosafety Cabinet (BSC) is a bench/table top model, optionally available with
a base support stand, for operation as a console model. The LABGARD ES model NU-427 utilizes an Energy Saver DC ECM
motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and filter
loading capacity. The Energy Saver ECM motor is controlled to airflow setpoints via a solid-state DC motor controller with
digital dual thermistor airflow sensors that provide an automatic compensation (constant volume control) for both filter
loading and line voltage variances.
The Laminar Flow Biosafety Cabinet, (BSC) is a product resulting from the development of the "laminar flow" principle and
the application of environmental controls as required in the field of biological research or chemical containment. The BSC,
when used with proper technique, is an effective laboratory aid in obtaining the optimum control over product quality
while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical
agents in low to moderate risk-hazard research and use of the BSC to work with chemicals and trace amounts of
radionuclides required as an adjunct to microbiological studies, as prescribed by the Center for Disease Control (CDC)
Atlanta, Georgia.
The NU-427 Bench LFBSC meets the requirements of a Class II, Type B1 since the cabinet conforms to the following
requirements:
•Maintain a minimum average inflow velocity of 100 fpm (0.51m/s) through the work access opening;
•Have HEPA filtered downflow air composed largely of uncontaminated re-circulated air
•Exhaust most of the contaminated downflow air to the atmosphere through a hard connection to the facility
exhaust system after filtration through a HEPA filter
•Have all contaminated ducts and plenums under negative pressure or surrounded by directly exhausted
(non-recirculated through the work area) negative pressure ducts and plenums
Type B1 cabinets may be used for work with volatile chemicals if permitted by a chemical risk assessment..