NuAire LabGard ES User manual

OM0292
LabGard® ES Energy Saver
Class II, Type A2 Animal Handling
Biosafety Cabinet
Models
NU-640-400E/500E/600E
Bench/Console
Operation and Maintenance Manual
November, 2021
Revision 4
NU-400E/500E/600E Series A
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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Congratulations!
You have just purchased one of the finest Animal Handling Biosafety Cabinets available. With proper
care, maintenance (certification), and laboratory procedure, this cabinet will give you years of product and
personnel protection from particulate contaminants as prescribed in NSF/ANSI 49. Please read this manual
carefully to familiarize yourself with proper installation, maintenance and operation of the cabinet. Other
reference and guideline materials are available through the following web sites;
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.cabs-acsb.ca
www.ebsaweb.eu
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.nuaire.com

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About this Operation and Maintenance Manual
The information contained in this manual is intended to reflect our current production model along
with the more frequently purchased options. Any unique additions, modifications, or shop drawings are
appended in the back flap of this manual, along with any modifications and/or additions to procedures as
outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this
manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be
obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description
of the information desired.

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LabGard® ES Energy Saver
Class II, Type A2 Animal Handling Biosafety Cabinet
Models NU-400E/500E/600E
Operation and Maintenance Manual
Table of Contents
Section No. 1.....................................................General Information
Section No. 2.....................................................Models and Features
Section No. 3.....................................................Warranty
Section No. 4.....................................................Shipments
Section No. 5.....................................................Installation Instructions
5.1..................................................................... Location
5.2..................................................................... Set-up Instructions
5.3..................................................................... Testing Methods and Equipment
Section No. 6.....................................................Operating the NU-640
6.1..................................................................... Aeromax™Control System
6.2..................................................................... Operating Guidelines
6.3..................................................................... Operating Sequence
6.4..................................................................... Ergonomics
6.5..................................................................... Cleaning Procedures
Section No. 7.....................................................General Maintenance
7.1..................................................................... Decontamination
7.2..................................................................... LED Lamp Bulb Replacement
7.3..................................................................... HEPA Filter/Motor Replacement
7.4..................................................................... Sliding Window Replacement and Manual Adjustment
7.5..................................................................... Airflow Control System Setup and Calibration
7.6..................................................................... HEPA Filter Leak test
7.7..................................................................... Airflow Smoke Pattern Test
7.8..................................................................... Site Installation Assessment Test
7.9..................................................................... Cleanliness Classification Test for Pharmacy Application
7.10................................................................... Main Control Board Description and Replacement
Section No. 8 ....................................................Error Messages, Troubleshooting and Option-Diagnostics
Section No. 9.....................................................Remote Contacts
Section No. 10...................................................Electrical/Environmental Requirements
Section No. 11...................................................Disposal and Recycle
Manual Drawings
CD-000031.......................................NU-640 Airflow Schematic
CD-000042.......................................NU-640-400E Specification Drawing
CD-000043.......................................NU-640-500E Specification Drawing
CD-000044.......................................NU-640-600E Specification Drawing
CD-000035.......................................NU-640 Drain Valve Installation
CD-16521.........................................Front Label
Electrical Schematics
CD-000037 (sheet 1 and 2)..............NU-640-400E/500E/600E Electrical Schematic

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LabGard® ES Energy Saver
Class II, Type A2 Animal Handling
Biosafety Cabinet
Models
NU-400E/500E/600E
Manufactured by:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LabGard® ES Model NU-640 Animal Handling Biosafety Cabinet (AHBSC) is a bench/table top model, optionally available
with a base support stand, for operation as a console model. The LabGard® ES model NU-640 utilizes an Energy Saver DC
ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and
filter loading capacity. The Energy Saver ECM motor is controlled by the Aeromax™ Control System to nominal airflow
setpoints via an integrated solid-state DC motor controller based on feedback algorithm to provide automatic
compensation (constant volume control) for both filter loading and line voltage variances. Airflow velocity is monitored by
the PresurFlow™ system with a digital pressure sensor.
The Animal Handling Biosafety Cabinet, (AHBSC) is a product resulting from the development of the "laminar flow" principle
(see Airflow Schematic) and the application of environmental controls as required in the field of biological research or
chemical containment. The AHBSC, when used with proper technique, is an effective primary engineering control in
obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne
biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product
operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-640 bench AHBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening.
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum.
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration.
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure.
Cabinets used for work with minute quantities of non-flammable or explosive volatile toxic chemicals and tracer amounts of
radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust
canopies.

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1.2 Safety Instructions
These safety instructions describe the safety features of the LabGard® ES Model NU-640 AHBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested
before delivery. However, the cabinet may present potential hazards if it is not installed and used as instructed for its
intended purpose or is used outside of operating parameters. Therefore, the following procedures must always be
observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this cabinet, the operator must prepare clear and concise written instructions for operating
and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular.
owhich decontamination measures are to be applied for the cabinet and accessories
owhich protective measures apply while specific agents are used
owhich measures are to be taken in the case of an accident
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the cabinet so that safety instructions and important information are
always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.
1.3 Explanation of Symbols
NOTE:
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation
which, if not avoided, may result in property
damage.
Potential electrical hazard, only qualified person
to access.
Used for important information.
Biohazard
Ground, Earth
CAUTION

Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Lead Free
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death
of serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!

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2.0 Models and Features
The model NU-640, LabGard® ES, Class II, Type A2 Animal Handling Biosafety Cabinet is manufactured
in three sizes: 4 ft. (1.2m) , 5 ft. (1.5m) , and 6 ft. (1.8m).

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3.0 Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
Within the data packet sent with the unit.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your LabGard® ES cabinet arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LabGard® ES cabinet to
be sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.

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5.0 Installation Instructions
5.1 Location
Within the laboratory, pharmacy, etc., the ideal location of the Biosafety cabinet is away from personnel traffic lanes, air
vents (in or out), doors and/or any other source of disruptive air currents.
Suggested Cabinet Location in Laboratory
THE EXHAUST FILTER AREA IS ESPECIALLY SUSCEPTIBLE TO DISRUPTIVE AIR CURRENTS FROM AIR VENTS. The exhaust
sensor for the Electronic Airflow Control System is located just above the exhaust HEPA filter and if disruptive air currents
are present, the exhaust sensor could be influenced by them, and indicate disruptive readings on the front panel display.
If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential
exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current.
REMEMBER: A BIOSAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.
Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance purposes.
The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. Do not
position the cabinet to prevent access to the power cord. The power cord plug serves as the disconnect and should
remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an appropriate
warning label should be applied near the cabinets on/off switch to indicate the circuit breaker on the power distribution
panel should be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET TO THE
CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.
If this cabinet is used in a pharmacy application, it is strongly recommended (Per OSHA, NIOSH and ASHP) that the cabinet
be exhausted to the outside. In addition, if this cabinet is used in microbiological application with minute quantities of
volatile toxic chemicals and trace amounts of radionuclides, per CDC/NIH and NSF, it is strongly recommended that the
cabinet be exhausted to the outside. NuAire offers a canopy type of exhaust transition, which will capture the exhaust
efflux from the cabinet.
Note: The Exhaust System should be fitted with a backdraft damper to prevent reversing of airflow in the system.
NuAire only recommends a canopy or thimble transitions for exhausting applications. See separate instruction sheets for a
discussion of exhaust transitions and installation requirements.


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5.2 Set-Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually
the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate
ease in handling. It can then be removed from the skid by removing the banding, bolts and screws holding the
cabinet to the skid. The castors may then be attached. The cabinet should be pushed off the skid attaching the
castors on each corner as the castor base plates hang over the skid. IT IS RECOMMENDED THAT NO LESS THAN
TWO PEOPLE PERFORM THE SKID REMOVAL AND CASTOR ATTACHMENT PROCESS.
It is recommended that no less than two people are present using a
lifting system for placement of the cabinet onto the base stand.
It is not recommended to manually lift the cabinet onto the base stand.
5.2.1 Gas Service
NuAire doesn't recommend the use of natural gas within the AHBSC, but if gas service is determined to be
necessary for the application, appropriate safety measures must take place. All NuAire AHBSC's have
precautionary warning labels that say the following:
Use of explosive or flammable substances in this cabinet
should be evaluated by your appropriate safety personnel.
Once the determination has been made by the appropriate safety personnel, the application of natural gas must
be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE AHBSC ON THE GAS SUPPLY LINE.
All NuAire’s AHBSC’s meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with these
safety requirements, NuAire uses only certified gas valves. If external piping is required, only black pipe is used for
this application.
As previously stated NuAire doesn't recommend the use of natural gas within the AHBSC and ASSUMES NO
RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the AHBSC not only
contributes to heat build-up; it also disrupts the laminar air stream, which must be maintained for maximum
efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS
STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner
should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect.
CAUTION
!
CAUTION
!

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5.2.2 Plumbing Services
Service ball valves with the type of service specified by the removable button on the handle are located in the
work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR). Reducing valves
should be installed external to the cabinet if necessary. Service ball valves should never be used for flammable
gasses or oxygen service. A special needle valve for oxygen service or certified valve is required and available upon
request.
External connection is to 3/8 inch NPT coupling in the inner sidewalls. Connection to plant utilities should be made
with proper materials for the individual service and according to national and/or local codes. Observe all labels
pertaining to the type of service and operating pressure.
5.2.3 Electrical Services
The NU-640 series Biosafety Cabinets are plugged into an outlet with protective earthing connection with the
standard power cord. The cabinet requires 115 VAC, 60 Hz, single phase (current rating varies per cabinet size,
reference Electrical/Environmental Requirements). It is recommended that power to the cabinet, whether
hardwired or plug connected, be on its own branch circuit, protected with a circuit breaker at the distribution
panel near the cabinet. A surge protector is strongly recommended if you are experiencing power related faults.
NOTE: THIS CABINET CONTAINS ELECTRONIC BALLASTS FOR THE LED LIGHTING. ELECTRONIC BALLASTS
OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH
GROUND FAULT CIRCUIT INTERRUPTERS (GFCI’S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO
TRIP.
5.2.4 Final Assembly
NOTE: Remove the protective cardboard cover over the exhaust HEPA filter.
Remove the protective cardboard cover over the exhaust HEPA filter located under the protective screen if in
place. The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a
soft cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used.
Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any
mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before
using it as a AHBSC.



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5.3 Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be tested or commissioned to factory standards.
As part of testing, the certifier should go through the following initial checklist to assure all aspects of the AHBSC installation
are complete and ready for testing.
•Review product installation
- Exhaust connection, if present
- Damper valve installed correctly with label toward front, if present
- AHBSC base stand level
•Perform AHBSC certification
- At a minimum, the following tests should be performed:
•HEPA filter leak test
•Downflow velocity test
•Inflow velocity test
•Airflow smoke patterns
•Site installation assessment tests
The testing methods and equipment required are specified on the factory inspection report included with this manual (see
insert in back cover).
NOTE: IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR
WITH THE METHODS AND PROCEDURES FOR TESTING BIOSAFETY CABINETS (SEE INSERT).
NOTE: AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM OF AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE, MAINTENANCE ACTION, OR ANY
TIME THE OPERATOR FEELS IT IS NECESSARY.
Note that the LabGard® ES cabinets, filters and seals provide premium performance; Quality Control in both design and
manufacturing assure superior reliability. However, protection to both product and operator is so vital that installation
testing to the performance requirements should be accomplished as stated to ensure Biosafety established by the factory
standards.



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LabGard® ES Energy Saver
Class II Type, A2 Animal Handling Biosafety Cabinet
Models NU-400E/500E/600E
Catalog Number
Catalog Number
NU-640-400
Nominal 4 foot (1.2m)
NU-640-500
Nominal 5 foot (1.5m)
NU-640-600
Nominal 6 foot (1.8m)
Performance Specifications
1. Personal Protection
2. Product Protection
NSF/ANSI 49
NSF/ANSI 49
NSF/ANSI 49
NSF/ANSI 49
Class II, Type A2
Class II, Type A2
Class II, Type A2
Style of Cabinet
console
console
console
Cabinet Construction
All welded stainless steel
16/18 gauge, Type 304
pressure tight design
All welded stainless steel
16/18 gauge, Type 304
pressure tight design
All welded stainless steel
16/18 gauge, Type 304
pressure tight design
Diffuser for Air Supply (Metal)
Non-flammable
Non-flammable
Non-flammable
HEPA Filter Seal Type:
Supply Filter-99.99% Eff. on 0.3 microns
Exhaust Filter-99.99% Eff. on 0.3 microns
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
Fumigation : per NIH/NSF Procedures
Yes
Yes
Yes
Standard Services:
Duplex Outlet
Two Backwall
Two Backwall
Two Backwall
Optional Services:
Gas Cocks 3/8" NPT
Up to 3 ea. Sidewall
Up to 3 ea. Sidewall
Up to 3 ea. Sidewall
Cabinet Size Inches (mm):
Height (minimum)
Height (maximum)
Width (w/side push/pull bars)
Depth
78 1/4 (1987)
86 1/4 (2190)
56 3/8 (1433)
33 (838)
78 1/4 (1987)
86 1/4 (2190)
68 3/8 (1737)
33 (838)
78 1/4 (1987)
86 1/4 (2190)
80 3/8 (2042)
33 (838)
Work Access Opening Inches (mm):
Standard Opening Height
Standard Inflow Velocity
14 (356)
105 FPM (.53 m/s)
14 (356)
105 FPM (.53 m/s)
14 (356)
105 FPM (.53 m/s)
Work Zone Inches (mm):
Height
Width
Depth
24 5/8 (626)
46 1/2 (1181)
22 3/4 (579)
24 5/8 (626)
58 1/2 (1486)
22 3/4 (579)
24 5/8 (626)
70 1/2 (1791)
22 3/4 (579)
Window view height Inches (mm):
Window Type
23 7/8 (607)
Safety Glass/Hinged
23 7/8 (607)
Safety Glass/Hinged
23 7/8 (607)
Safety Glass/Hinged
Required Exhaust CFM/CMH (opening)
Standard/Optional:
Canopy Variable Flow Thimble (NU-911)
Canopy Fixed Flow Thimble (NU-926)
14 (356)
CFM (CMH)
575 - 725 (977 - 1232)
609 (1035)
14 (356)
CFM (CMH)
699 - 829 (1188 - 1408)
731 (1242)
14 (356)
CFM (CMH)
880 - 970 (1495 - 1648)
888 (1509)
Plant Duct Static Pressure Eng./Metric
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
Heat Rejected, BTU, Per Hour (opening)
(non-vented)
(vented)
14 (356)
1883
198
14 (356)
2197
198
14 (356)
2354
198
Electrical:
Volts, AC 60 Hz
+Amps: Blower/Lights
Amps: Duplex
Rated Amps:
12 ft. Power Cord (one)
U.L./U.L.-C Listed
115
4.8
3
14
12 gauge - 3 Wire, 20A
U.L./U.L.-C Listed
115
5.6
3
16
12 gauge - 3 Wire, 20A
U.L./U.L.-C Listed
115
6.0
3
16
12 gauge - 3 Wire, 20A
Crated Shipping Weight:***
Net Weight
610 lbs. /277 kg.
560 lbs. /254 kg.
700 lbs. /318 kg.
650 lbs. /295 kg.
790 lbs. /358 kg.
740 lbs. /336 kg.
***Crated shipping weight does not include weight for accessories or options + Based on cabinet with new filters running at 115VAC.

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6.0 Operating the NU-640
6.1 Aeromax™ Control System
6.1.1 Overview
The Aeromax™ control system is designed to service the control requirements of the LabGard® ES NU-640
Biosafety Cabinet. The Aeromax™ control system consists of an electronic module that will perform the following
functions:
•Easy user interface via LED’s and function keys
•Control blower via solid state switch.
•Control lights via solid state switch.
•Control outlets via solid state switch.
•Disable audible alarm switch with ring back function.
•Control blower DC ECM motor with solid-state DC Motor Controller that provides automatic
compensation (constant volume control) for both filter loading and line voltage variances.
•Monitor and display airflow system performance via PresurFlow™ monitor.
The LabGard® ES NU-640 offers the latest digital microprocessor design technology for improved cabinet
performance and safety. The Aeromax™ control system integrates a digital pressure sensor (PresurFlow™) to
monitor the cabinet’s airflow performance. The Aeromax™ control system also integrates a DC motor controller
that provides automatic compensation for both filter loading and line voltage variances. There is additional on/off
control of blower, LED light, and outlets. All the above functions are shown in a system block diagram (see figure
1).
Figure 1

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