Okolab H301-ALPHA3-LARGE User manual

H301-ALPHA3-LARGE User Manual
REV 01
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H301-ALPHA3-LARGE
H301-ALPHA3-LARGE is compatible with PhaseView Alpha3Light Sheet microscope.
IST 2068_REV01

H301-ALPHA3-LARGE User Manual
REV 01
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Index
1. Components and dimensions................................................................................................... 3
2. Installation of the Chamber around the sample holder................................................................... 4
3. Connection of the Gas Supply ................................................................................................. 6
4. Working with perfusion ......................................................................................................... 6
5. Insertion of the Sample Feedback Temperature Sensor.................................................................. 7
6. Cleaning ........................................................................................................................... 8

H301-ALPHA3-LARGE User Manual
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1. Components and dimensions
H301-ALPHA3-LARGE includes the following components:
•Objective Collar, compatible with water immersion objective 21 mm diameter.
•Sample Arm Cover
•Chamber Lid
•Chamber Base
Chamber Base
Objective
Collar
Chamber Lid
Sample Arm
Cover
Figure 1. H301-ALPHA3-LARGE –Components and dimensions.

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2. Installation of the Chamber around the sample holder.
To install the H301-ALPHA3-LARGE around the sample holder, please follow the steps below:
1. Insert the chamber base by sliding into place as shown in image 1 and 2 in Figure 2.
Note: Please pay attention to the sample holder glass.
2. Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided 1.5 mm
hexagonal key (see image 2 in Figure 2).
3. Place the magnetic lid on the chamber base (see image 3 in Figure 2).
4. Insert the sample arm cover (see image 4 in Figure 2), and close it (see image 5 in Figure 2). It allows to reduce
the brightness into the sample.
5. Place the magnetic objective heater in place as shown in image 6 in Figure 2.

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5
4
2
1
3
7
6
Figure 2. Chamber installation around the sample holder.

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3. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to H301-ALPHA3-LARGE. Silicon tubing con-
nects to a gas input - brass opening - located on a corner of the H301-ALPHA3-LARGE (see Figure 3). Connect by gently
pushing silicon tubing onto brass opening.
Gas Input
Figure 3.Connection with gas supply.
4. Working with perfusion
The Chamber H301-ALPHA3-LARGE features 2 perfusion holes on the sides for the insertion of perfusion tubing up to 1
mm in outer diameter. Follow the instruction below to work with perfusion:
6. Use the supplied Stainless Steel Perfusion Tubes. Attach a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to
the Stainless Steel Perfusion Tubes (see image 1 in Figure 4).
7. Insert the Stainless Steel Perfusion Tubes in the perfusion holes (see images 2 and 3 in Figure 4).
Insert a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to the free end of the Stainless Steel Perfusion Tubes
(see image 4 and 5 in Figure 4).

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4
2
1
3
Stainless Steel
Perfusion Tube
Silicon Tube
O.D.1,6 mm
I.D. 0,8 mm
(not supplied)
5
Perfusion
Hole
Figure 4. Working with Perfusion
5. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-ALPHA3-LARGE
(see Figure 5).
To read the temperature of the culture medium and realize a self-calibration of the chamber, fix the Temperature
Sensor tip with adhesive tape on the sample holder inside the H301-ALPHA3-LARGE. For more information about the
self-calibration, please refer to the User Manual of the Okolab Controller in use.

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1.Frontal view 2. Temperaure Sensor placement
Opening for Temperature Sensor
Sensor Tip
Adhesive Tape
Figure 5. Insertion of the temperature sensor inside the chamber.
6. Cleaning
•Unplug all electrical cables.
•Remove the chamber lid from the chamber main body, and keep it separate from the chamber base while the
chamber cools down.
•To clean the body of the chamber, wipe with a soft micro-fiber cloth. For stubborn smudges, you can damp
the soft micro-fiber cloth with ethyl alcohol (product code UN1170). Do not put any liquid directly on the
chamber.
H301-ALPHA3-LARGE_User Manual Rev 01_Published in April 2021