manuals.online logo
Brands
  1. Home
  2. •
  3. Brands
  4. •
  5. Olympus
  6. •
  7. Microscope
  8. •
  9. Olympus BX53 User manual

Olympus BX53 User manual



L
M
A.S
y
1. Si
2. T
u
3. T
u
X
e
4. S
t

B.H
a
1. Li
g
2. S
e
3. H
a
li
g
4. P
r
cl
k
n
T
h
b
u
M
WMicrosco
p
y
stemStart‐
u
gnintothel
o
u
rnon(orw
a
u
rnonthem
i
e
nonlamp
f

t
artcellSens
D
a
rdwareCon
t
g
htpathbar
s
e
lectanobje
c
a
logenlamp
g
htwillbevi
s
r
iorZ‐stage
C
osetotheo
b
n
ob;theexte
h
eselecteds
p
u
ttononthe
c
e
j
p
yLaborator
y
Oly
m
u
p.
o
gbookont
h
a
keup)thec
o
i
croscope
b
,
(onlyifyou
n
D
imension
t
rolandSett
i
s
g
directsli
g
c
tivelensby
t
dialknob
i
c
s
iblefromth
e
C
ontrolBox
d
b
jectiveand
D
ntofthefin
e
p
eedappear
s
controller.)
d
y

m
pus B
X
h
edesk(star
t
o
mputerand
themainco
n
n
eeditforflu
program.
i
ng.
g
httoeyepie
c
t
urningthen
o
c
ontrolsthe
b
e
condenserl
e
d
movesthe
s
D
OWNbutto
n
e
movement
c
s
ontheLED
s
X
53 Digi
t
t
/endtime).
select“USER
”
n
trolunit
c
,
t
orescencei
m
c
e,camera,
o
o
sepiecetur
r
b
rightnessof
e
ns.
s
tageupord
n
bringsitdo
w
c
anbeselect
e
s
creen(Note
:
f
t
al Micr
o
”
andentert
h
t
hePriorre
m
m
aging).
o
rboth.
r
et
h
byhan
d
transmissio
n
owntofocus
w
nquickly.
F
e
dHigh(H),
M
:
DON’TUSE
t
b
c
Oly
o
scope
hepassword
m
otez‐stage
c
d
.
n
lightforbri
g
s
onspecime
n
F
inermovem
e
M
edium(M),
t
hefocuskn
o
h
b

i
mpusB
X
User G
u
onthelog‐i
n
c
ontroller
d
,
g
htfieldview
n
.TheUPbu
t
e
ntofstage
c
orlow(L)by
o
bonthemi
c
c
X
53User
u
ide
n
window.
DP26colorc
a
ing.Thetra
n
t
tonbringst
h
c
anbecontr
o
pressing“SP
c
roscopean
d
g
Guide|
1
a
mera
e
,an
d
n
smission
h
estageup
o
lledwiththe
EED”button.
d
theZERO
g
1

d

g



L
M
5. R
e
b
u
o
p

C.O
v
1.
W
h
w
2.
S
s
m
o
3.
C
4.
F
p
M
WMicrosco
p
e
motecontr
o
u
ttonstosel
e
p
enorclose
t
v
erviewofc
e
W
henthepr
o
h
istochemica
l
w
indowtab
S
electanobj
e
s
tagewilllow
m
atchingobj
e
o
bjectivetoo
C
lickoneof
F
ocusontoth
p
anel.
1)
1!
p
yLaborator
y
o
llerbox
j
c
o
e
ctanappro
p
t
hemercury
a
e
llSenseprog
r
o
gramopens,
l
lystainedsa
m
.
e
ctive(start
w
erandames
e
ctiveinplac
e
btaincorrect
esampleby
y

o
ntrolsthes
e
p
riatefilterm
a
rclampligh
t
r
amandbas
i
firstsetupt
h
m
plesorflu
o
w
ith5xor10
x
sagewindo
w
e
.ClickOKo
scaleba
r
).
butto
n
movingstag
e
e
lectionofi
m
irrorcubefo
r
t
shutter.
i
cimageacq
u
h
eprogramf
o
rescenceim
a
x
objective)b
y
w
willpopup.
nthemessa
g
n
sontheOb
s
e
withPriorz
‐
m
agingmode
(
r
differentco
u
isition.
oryourimag
a
gingforfluo
r
y
pressingan
Turntheob
j
g
ewindow(I
t
s
ervationMe
‐
stagecontr
o
Oly
(i.e.BForFlu
lorsoffluore
ingneeds(i.
e
rescentlylab
e
objective
j
ectivenose
p
t
iscriticalto
m
thodspanel
f
o
llerorbycli
c
mpusB
X
u
orescence(F
e
scence.Pre
s
e
.bright‐field
e
ledsamples
p
ieceturret
h
m
atchthem
a
f
orbrightfiel
d
c
kingarrowh
e
X
53User
L)).Usethe
M
s
stheFLShu
t
imagingfor
).Clickthe
A
h
byhandto
p
a
gnification
w
d
orfluoresc
e
e
adsintheD
e
Guide|
2
M
irror
t
terbuttont
o
A
cquisition

button.Th
e
p
utthe
w
iththe
e
nceviewing.
e
viceUnits
2

o

e




L
M
5.
S
b
A
6.
S
(

D.B
1.
M
t
2.
C
(
w
3.
W
a


M
WMicrosco
p
S
electacam
e
b
rightfield.
S
A
utomaticfr
o
S
electResolu
t
(
active)scen
e
rightfield(o
r
M
akesureth
a
t
heupperlig
h
C
lickLive
Automatice
x
w
orkfinefor
W
hentheset
a
s.vsior.tiff
o
1$
1%
p
yLaborator
y
e
rafromthe
C
S
electManu
a
o
mthewhite
t
ion
1!
asde
s
e
.)
r
DIC)Image
a
tDP26cam
e
h
tpathbar
1
@
button
x
posure
1$
w
mostcases).
t
ingissatisfa
o
rmatbyclic
y

C
ameralist
1
)
a
lorAutoma
balancemo
d
s
ired.(Note:
Y
Acquisition
e
raisONand
@
ispushedin
andobtain
p
ithSpot30%
Clickthebu
t
ctory,clickS
n
kingtherigh
t
)
:XM10mon
o
ticexposure
d
elist.
Y
oucanchoo
s
selectedfro
m
andthelow
e
p
roperexpos
u
regionanda
t
tonagainto
n
ap
t
mousebutt
o
o
chromeca
m
toobtainbe
t
s
ealowerre
s
m
thecamer
a
e
rbar1
1#
is
p
u
reandwhit
e
utomaticw
h
stoplivevie
w
buttonands
o
noverthei
m
Oly
m
eraforfluo
r
t
terimages.
W
s
olutionforL
a
listandtha
p
ulledout.
e
balance
h
itebalance
1
%
w
ing.
avetheima
g
m
agetab.
mpusB
X
r
escenceor
D
W
ithDP26c
a
L
ive/Movie
fo
t
%

g
e
X
53User
D
P26colorca
m
a
meraselect
e
o
rfas
t
updat
e
1@
1#
Guide|
3
m
erafor
e
d,select
e
oflive
3



L
M
E.Fl
1.
C
o
2.
S
one
o
posi
t
and
t
M
WMicrosco
p
uorescenceI
C
licktheFLb
u
o
noroffbyp
S
electanapp
o
ftheObser
v
t
ion
e
.Mak
e
t
helowerba
r
d
p
yLaborator
y
mageAcqui
s
u
tton
b
ont
h
ressingFLSh
ropriatefluo
r
v
ationMeth
o
e
surethatth
e
r
pushedin)
a
c
y

s
ition
h
eremotec
o
utterbutton
c

r
escencemir
r
o
dsbuttons
e
lightpath
b
a
ndXM10ca
m
b
o
ntrollerbox
t
c
onthere
m
r
orcubebyp
b
ars
f
arein
m
eraisselec
t
t
ousemercu
m
otebox.
ressingMirr
o
(exce
p
correctposi
t
t
ed.
Oly
ryarclampl
i
o
rbuttons
D
p
tBFandDIC
t
ion(theupp
e
e
mpusB
X
i
ghtforfluor
e
inthecontr
o
C
).Theselect
e
rbaratthe
X
53User
e
scence.Th
e
o
llerboxorcl
edmirrorwil
midorexten
d
f
Guide|
4
e
lightcanbe







ickingany
lbein
dedposition
b
4



L
M
3.L
e
and
T

4. Cl
m
t
h
n
e
b
u
e
x
c
o

5. Cl
o
p
c
h
f
o
a
s
S
a

6. I
m
t
o
a
s
s
a

7. S
c
di
i
m















M
WMicrosco
p
e
t’stakemul
t
T
xRedfluoro
p
l
icktheProc
e
m
ulticolorwh
e
h
elist.Repe
a
e
xttotheca
m
u
tton.IfAut
o
x
posuretime
o
nditionsfor
l
icktheFITC
s
p
timalexpos
u
h
annel.Once
o
reachchan
n
s
.vsifileint
h
a
vetheorigi
n
m
agescanals
o
changethe
i
s
oneimage
c
a
vetheimag
e
c
alebarcan
b
i
splayedwith
m
age.Savet
h
p
yLaborator
y
t
icolorfluore
s
p
hores.
e
ssManager
t
e
el butto
n
a
ttwicetose
l
m
eraicon;it
w
o
maticexpos
.Oncethee
x
DAPI.
s
ectiononth
e
u
retimeand
allthesettin
n
elautomatic
h
eLibraries>
P
n
alimagefile
s
obesaveda
s
i
mageinto8
‐
c
ontainingm
u
e
asmultiple
i
b
eviewedwi
t
View/InfoS
t
h
eannotated
y

s
cenceimag
e
t
ab
f
andch
n
.Clickthe
l
ectFITC(gre
w
illexpandt
o
ure(withSp
o
x
posurecon
d
e
listanduse
clickico
n
g
sareobtain
ally.Theres
u
P
icturesfold
e
s
as.vsiform
a
s
.tifffiles.S
e
‐
bitimage.U
s
u
ltiplechann
e
i
magesfore
a
t
hView/
S
cal
e
t
ampcomm
a
imageasJP
E
e
acquisition
w
ecktheAuto
en)andTxRe
o
showcurre
n
o
t30%)isus
e
d
itionsarese
t
theAutoma
t
n
toreadthe
ed,clickthe
u
ltingmultic
h
e
rassoonas
t
a
twithouta
n
e
lectImage>
M
s
eFile>Save
a
e
ls.OruseFi
l
a
chchannel.
e
barcomma
a
nd.Thesea
n
E
G(JFIF)(*.jpg
w
ithanexam
maticProce
s
butto
n
d(red)chan
n
n
texposure
s
e
d,letthepr
o
t
,clickic
t
icexposure.
setting.Rep
but
t
h
annelimage
t
heacquisiti
o
n
ymanipulati
o
M
ode>8bit/
C
a
s…menuco
l
e>Exportto
>
ndandthe
m
n
notationsc
a
).
Oly
plestained
w
s
ses
g
.Click
n
andselect
D
n
els.Clickth
e
s
etting.Click
o
gramfindth
ontosetthe
Waituntilit
f
eatoncemo
r
t
on.Itwillta
e
,bydefault,
w
o
nhasbeenc
onorproces
s
C
hannelmen
mmandtos
a
>
tiffseries…
c
m
agnification
a
n’tbesaved
mpusB
X
w
ithDAPI,FI
T
k
the
D
API(blue)in
e
“plus”sign
theLive
eappropriat
e
seexposure
f
indsan
r
eforTxRED
kethreesho
t
w
illbesaved
c
ompleted.
s
ing.
n
ucommand
a
vetheimag
e
c
ommandto
infocanbe
withyourtif
f
X
53User
T
C,
e

t
s
e

f

f
Guide|
5
g
5



L
M
F.I
m
1. O
o
r
hi

2. I
n
D
i
o
u
u
n












M
WMicrosco
p
m
ageProces
s
nceyousave
r
publication.
i
stogramand
n
default,the
i
mensionSel
u
teyesymb
o
n
wantedcha
n
p
yLaborator
y
ing
theoriginal
f
ClickthePr
o
thebrightne
adjustment
o
ectorpanel
b
o
lindicates“i
n
n
nels.Then,
y

f
ile,makea
d
o
cessingwin
d
ssandcontr
a
o
ftheHistog
r
b
,youcanch
n
visible”).Cl
i
youcanadju
uplicateifit
i
d
owtab
a
stofthedis
p
r
amortheDi
oosethecha
i
ckthecolor
c
stthehistog
r
i
snecessary
t
fro
m
p
laycanbea
d
splayEnhan
c
nnelstobev
c
hannelsyou
r
amandthe
b
Oly
t
omakeany
c
m
themainwi
d
justed.
c
ementappli
v
isible(eyesy
wanttosho
w
b
rightness/c
o
mpusB
X
c
hangesinth
i
ndowtabs,i
n
estoallthe
v
mbolind
i
w
andclicko
o
ntrastofth
e
X
53User
eimagefor
p
n
whichthei
m
v
isiblechann
e
i
cates“visibl
e
ntheeyesy
m
e
selectedch
a
Guide|
6
p
resentation
m
age
e
ls.Onthe
e
”andgrey‐
m
bolstohide
a
nnelonly.
b
6




L
M
3. 
T
o
p

(
(
(













M
WMicrosco
p
T
osavesome
p
erationswil
l
1) Makeac
o
Edit/Pas
t

2) Makeac
o
Thiswill
c

3) Selectall
multicha
n
selectch
a
c
p
yLaborator
y
overlaidima
g
l
automatica
l
o
mpositeim
a
t
eAs/NewI
m
o
mpositeim
a
c
reateanew
i
channelsfro
m
n
nelimagein
t
a
nnelstobe
c
y

g
esfromam
l
lychangeth
e
a
geasabove.
m
age.Thiso
p
a
geasabove.
i
magewiths
c
m
theDimen
s
t
oindividual
c
ombinedan
d
ultichanneli
m
e
imagetype
f
FromtheEd
p
erationwill
g
UseEdit/Co
p
c
alebarinpi
x
s
ionSelecto
r
channelima
g
d
clickOK.
m
ageyoujus
t
f
rom16bitg
itmenu,use
g
enerateaco
p
yDisplayto
x
elunit(losin
r
.UseImage
/
g
es.Thenus
e

Oly
t
madeasab
o
g
rayscaleto8
Edit/Select
A
mpositeima
g
o
Clipboar
d
a
n
gsomedime
/
Separate/C
h
e
Image/Co
m
mpusB
X
o
ve,therear
bitcolo
r
):
A
ll,thenEdit
/
g
ewiththes
a
n
dthenEdit
/
e
nsionalinfor
h
annelscom
m
m
bineChann
e
X
53User
ethreeways
/
Cop
y
,andt
h
a
mescaleba
/
PasteAs/Ne
w
mation).
m
andtosep
a
e
ls
c
.Inthe
w
Guide|
7
(note:these
h
en
r.
w
Image.
a
ratethe
w
indow,
7



L
M
F.A
c
1. A
s
t
m
P
r
cl
b
r
b
u
p
o
2. T
h
o
b
It
3. Cl

G.D
i
1. I
n
2.
W
fr
3. Y
o
m
F
r
4. Y
o
b
u
St
T
h
cl
c
o
M
WMicrosco
p
c
quisitionof
Z
z‐stackimag
e
t
ackbu
t
m
ovethestag
e
r
ocessMana
g
osetotheco
r
ingupthest
u
tton
d
,whi
c
o
sition.)
h
eprogram
w
b
jective.Clic
willcalculat
e
l
icktheStart
i
splayingan
d
n
theProcess
i
W
iththenavi
g
amesofaz‐s
o
ucanselect
m
ousebutton
r
amefromth
o
ucanalsos
e
u
ttons
g
;de
t
t
artingPositi
o
h
enchoose
T
icktheright‐
m
o
ntainingonl
y
coverslip
Slide
p
yLaborator
y
Z
‐stack(z‐ser
e
containsm
u
t
tontodispla
y
e
usingtheP
r
g
erwindow.
verslipand
t
agetofocus
c
hwilltheSt
a
w
illautomati
c
kApplybutt
o
e
thenumber
buttontoca
p
d
Converting
z
i
ngtab,thez
‐
g
atorbar
tack.
asinglefra
m
inasinglefr
a
econtextm
e
e
lectashort
r
t
ermineane
w
o
nandchoo
s
Ti
leView.M
a
m
ousebutto
n
y
selectedfr
a
y

ies)
u
ltipleframe
s
y
Zstackpan
r
iorstageco
n
First,lower
t
henclickSet
ontoadeep
e
a
rtposition.
(
c
allyrecomm
e
o
ntoaccept
t
ofz‐slices
f

p
tureaz‐seri
e
z
‐stacks
‐
stackimage
m
efromaz‐st
a
meviewan
d
e
nu.
r
angeoffra
m
w
startingpo
s
s
eanewend
a
kesureallt
h
n
andselect
E
a
mewillbeg
e
Objectiv
e
s
acquiredat
el.Tosetth
e
n
trollerorby
t
hestagetof
o
button
c
,w
h
e
rregionclos
e
(
Imageacqui
s
e
ndastepsi
z
t
hisstepsize
accordingly.
e
s.Youcanc
o
canbemani
p
ackbyclicki
n
d
selectingE
x
m
eswithinth
e
s
itionwithin
t
positionand
echannelsa
r
E
xtrac
t
from
t
e
neratedas
a
e

differentfoc
e
StartandE
n
clickingthe
a
o
cusontoar
h
ichwillbet
h
e
totheslide
s
itionwillbe
g
z
e
e
forz‐se
r
orchangeas

o
mbinethis
f
p
ulatedinva
r
n
gtheright‐
x
tractCurren
t
e
stackbyusi
n
t
hestackan
d
clickSet
S
el
e
r
evisible(sel
t
hecontext
m
.vsifile.Sav
e
Oly
uspositions.
n
dlimitsofa
a
rrowheads
b
r
egionofinte
h
eEndpositi
o
andclickSe
t
g
inattheSta
r
iesaccordin
g
youwantan
f
unctionwith
r
iouswaysto
t

ngSetSelect
d
click
S
etSel
e
e
ctionEndP
o
ected).Over
m
enu.Ane
w
e
theimage.
End
Start
onstag
e
mpusB
X
Clickz‐
z‐stack,
b
inthe
rest
on.Next,
t

r
t

g
tothe
dapply.
multicolori
m
generatedi
f
,youc
a
ion
e
ction
o
sition.
animage,
w
z‐stack
e
e
g
X
53User
m
aging.Sav
e
f
ferentviews
.
a
nnavigated
e

f
Guide|
8
e
theimages.
.

ifferentfocal
b
c
d
8




L
M
5. Y
o
fr
6.
W
a
n
7.
W
di











M
WMicrosco
p
o
ucanproje
c
omthemain
W
iththe
S
lice
n
ywhereby
m
W
iththeslice
v
i
mensionvie
w
p
yLaborator
y
c
tallthez‐st
a
menuorM
a
Viewmenu,
y
m
ovingthex
y
v
iewmenuo
p
w
ofextende
d
y

a
ckframesin
t
ax
imumProj
e
y
oucandispl
y
cross‐hairlin
p
tions
d
x‐yslicepo
s
t
oasingle2
D
e
ctionOverZ
ayacross‐se
c
etothedesi
r
s
ition.
D
imagebyse
l
onthenavig
a
c
tionalz‐dim
e
r
edposition.
,suchas
Oly
l
ectingProce
s
a
torbarmen
ensionview
o

ExtendedSli
c
mpusB
X
ss/Projectio
n
u.
o
fanxandy
ce ,
X
53User
n
s/Maximu
m
plane.Youc
a
,
youcanvisu
Guide|
9
m
Zcommand
a
nview
alizez
9



Other manuals for BX53

1

Other Olympus Microscope manuals

Olympus SZX7 User manual

Olympus

Olympus SZX7 User manual

Olympus DP71 User manual

Olympus

Olympus DP71 User manual

Olympus BX-URA2 User manual

Olympus

Olympus BX-URA2 User manual

Olympus BH2 Series Installation instructions

Olympus

Olympus BH2 Series Installation instructions

Olympus U-D7RES User manual

Olympus

Olympus U-D7RES User manual

Olympus BXFM User manual

Olympus

Olympus BXFM User manual

Olympus SZX-AR1 User manual

Olympus

Olympus SZX-AR1 User manual

Olympus FV5-LD405 User manual

Olympus

Olympus FV5-LD405 User manual

Olympus BX51 User manual

Olympus

Olympus BX51 User manual

Olympus BX-RLA2 User manual

Olympus

Olympus BX-RLA2 User manual

Olympus IX70 User manual

Olympus

Olympus IX70 User manual

Olympus CHD User manual

Olympus

Olympus CHD User manual

Olympus BH2-5RE Installation instructions

Olympus

Olympus BH2-5RE Installation instructions

Olympus MIC-D Operating instructions

Olympus

Olympus MIC-D Operating instructions

Olympus BHTP User manual

Olympus

Olympus BHTP User manual

Olympus CH30 User manual

Olympus

Olympus CH30 User manual

Olympus MIC-D User manual

Olympus

Olympus MIC-D User manual

Olympus BX51-P User manual

Olympus

Olympus BX51-P User manual

Olympus SZ3060 User manual

Olympus

Olympus SZ3060 User manual

Olympus SZX16 User manual

Olympus

Olympus SZX16 User manual

Olympus SZ4045TR User manual

Olympus

Olympus SZ4045TR User manual

Olympus BH2 Series User manual

Olympus

Olympus BH2 Series User manual

Olympus GX53 User manual

Olympus

Olympus GX53 User manual

Olympus DP73 User manual

Olympus

Olympus DP73 User manual

Popular Microscope manuals by other brands

Graham Field BR3088F series instruction manual

Graham Field

Graham Field BR3088F series instruction manual

Levenhuk MED Series user manual

Levenhuk

Levenhuk MED Series user manual

Celestron 44120 instruction manual

Celestron

Celestron 44120 instruction manual

Bresser National Geographic Kids 300X/1200X MICROSCOPE... operating instructions

Bresser

Bresser National Geographic Kids 300X/1200X MICROSCOPE... operating instructions

3B SCIENTIFIC PHYSICS 1005442 instruction manual

3B SCIENTIFIC PHYSICS

3B SCIENTIFIC PHYSICS 1005442 instruction manual

Levenhuk DTX 30 user manual

Levenhuk

Levenhuk DTX 30 user manual

KERN Optics OBL-14 User instructions

KERN Optics

KERN Optics OBL-14 User instructions

Optika Italy B-510 Series instruction manual

Optika Italy

Optika Italy B-510 Series instruction manual

Bresser MicroSet 40x-1024x operating instructions

Bresser

Bresser MicroSet 40x-1024x operating instructions

Dino-Lite AM313 user manual

Dino-Lite

Dino-Lite AM313 user manual

Zeiss Axioscope 5 Quick reference guide

Zeiss

Zeiss Axioscope 5 Quick reference guide

american optical AO Series Reference manual

american optical

american optical AO Series Reference manual

Swift SW350 Series user guide

Swift

Swift SW350 Series user guide

Optika Italy B-380 Series instruction manual

Optika Italy

Optika Italy B-380 Series instruction manual

Zeiss Cell Observer SD operating manual

Zeiss

Zeiss Cell Observer SD operating manual

Optika Italy B-380 Series instruction manual

Optika Italy

Optika Italy B-380 Series instruction manual

Bodelin technologies ProScope Mobile instruction manual

Bodelin technologies

Bodelin technologies ProScope Mobile instruction manual

ViTiny UM08-CSZ1236 user guide

ViTiny

ViTiny UM08-CSZ1236 user guide

manuals.online logo
manuals.online logoBrands
  • About & Mission
  • Contact us
  • Privacy Policy
  • Terms and Conditions

Copyright 2025 Manuals.Online. All Rights Reserved.