Olympus CHA-P User manual

OLYMPUS POLARIZING MICROSCOPE
no CHAP

This instruction manual has been written for the use of the Olympus Polarizing Micro-
scope Model CHA-P. It is recommended to read the manual carefully in order to
familiarize yourself fully with the use of the microscope on the polarizing attachment
so that you can obtain the best performance and effectiveness.
IMPORTANT
Observe the following points carefully:
m Operation
1. Always handle the microscope with the care it deserves, and avoid abrupt motions.
2. Avoid exposure of the microscope to direct sunlight, high temperature and humidity,
dust and vibration.
If the microscope is used in ambient temperature higher than 40°C (104°F), it may
(ide its proper function. )
3. Only use the tension adjustment ring for altering the tension of the coarse adjustment.
Do not twist the two coarse adjustment knobs in the opposite directions simultane-
ously, which might cause damage.
4. Ascertain that the line voltage selector switch on the base plate is set to conform with
the local mains voltage.
= Maintenance
1. Lenses must always be kept clean. Fine dust on lens surfaces should be blown or
wiped off by means of an air blower or a clean brush, Carefully wipe off oil or finger-
prints deposited on the lens surfaces with gauze moistened with a small amount of
xylene, alcohol or ether.
2. Do not use organic solutions to wipe the surfaces of various components. Plastic
Parts, especially, should be cleaned with a neutral detergent.
3. Never diassemble the microscope for repair.
4. The microscope should be stored in its container immediately after use. If this is
not possible, it should be covered with a vinyl dust cover.
5. Disconnect the line cord from the AC power source before fuse replacement.

νι.
VII.
CONTENTS
STANDARD EQUIPMENT... 44444444 i ci va va ela e lee 2
NOMENCLATURE: : saxo es 44 Si gu sar va va alam vü sales vü 3
ASSEMBLY os ee morose nue sin ez iü iy Gele da e da 8 4
IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS ........ 5
OPERATION 7
a Adjustment of Minimum Line Voltage
2. Interpupillary Distance and Diopter Adjustments
3. Polarizer Alignment. > srmn su ema ge Ka guns ale Rw ee es BE Be 8
4. Centering the Stage ...................................... 9
δι Centering the Objectives
6. Use of Aperture Iris Diaphragm
% Focusing Adjustment sus am s rra o o siirleri vi m ür öle 10
8. Orthoscopic Observation
9. Conoscopic Observation ..........................,........ 11
OPTICAL DATA 12
TROUBLESAOOTİNG sema sz erir e e esimi 5 en ο ο a 13

一 一 —
I. STANDARD EQUIPMENT
Model
CHA-P-051 CHA-P-651
Component
rc CHAR 1
Intermediate polarizing attachment AH-PA 1 1
Quarter wave plate (retardation 147.3my) AH-TP147 1 1
Sensitive tint plate (retardation 530 my) AH-TP530 1 1
Polarizing monocular tube (45°) CH-PMO 1 0
Polarizing binocular tube (30°) BH-PBI 0 1
Swing-out polarizing condenser BH-POC 1 1
Halogen lamp socket C-LSH-B 1 1
Halogen bulbs 6V 10WHAL 2 2
PO 4X 1 1
Objectives (strain-free) PO10X 1 1
PO40X 1 1
AH-WF10X 0 1
Eyepieces
AH-Micro WF10X 1 1
Spare fuses (0.5A for 100-110-120V or 0.3A for 220-240V) 2 2
Vinyl dust cover

II. NOMENCLATURE
Photo: Model CHA-P-651. Model CHA-P-051 is also available by modification of some
components.
Eyepiece
Observation tube
Revolving nosepiece
Intermediate polarizing
attachment
Microscope stand
Condenser
Base

Ill. ASSEMBLY
The picture below illustrates the sequential procedure of assembly. The numbers indicate
the assembly order of various components. Remove dust caps before mounting components.
Take care to keep all glass surfaces clean, and avoid scratching the surfaces.
Insert the objective 10X into © Evepiece
the fixed aperture of the nose-
piece.
™ © Observation tube
Quarter wave plate
E
- -
Sensitive
tint
@
Intermediate
polarizing
tube
pinta Attach the tube to the stand
acai =
© Objective | gerek
compensator
=
Condenser
©
— |
©
< ーー OF
Halogen
lamp
Socket
Aligning red dots, on con-
denser mount and condenser,
insert the condenser into the
mount from below.
Microscope stand
Clamping screw
with the letters "OLYMPUS"
facing in front of the micro-
scope.
Clamping screw
8) To AC outlet

IV.
IDENTIFICATION
AND
FUNCTION
OF
VARIOUS
COMPONENTS
Observation tube
clamping screw
Compensator plate
Analyzer positioning screw
Analyzer rotation screw BO.
Observation tube clamping screw
Nosepiece centering screw Se 7 =
Analyzer scale
Tension adjustment ring
Stage clip
Stage sae
Rotatable 360'
Coarse adjustment knob
Clamping screw for stage
rotation
Fine adjustment
knob
Graduated in incre-
ments of 24.
Polarizer positioning
screw
Main
switch
as
Rheostat trimmer screw
Sliding contro! lever
For continuously variable light
intensity.
After switching on, if necessary,
rotate this screw with a coin until
the bulb is dimly li
sliding control lever at minimum
Position.
Grounding terminal
Fuse holder
Line voltage selector switch
Set the switch to conform with
the local mains voltage.

Mechanical tube length adjust-
ment rings
Bertrand lens turret ring
Engraved letters "IN" for in.
sertion of Bertrand lens into
the light path; “OUT” for
removal of the Bertrand lens
from the light path.
Bertrand lens focusing ring
Condenser height
adjustment knob
Stage centering screw
Automatic pre-focusing lever
Aperture iris diaphragm ring
Numerical aperture scale grad-
uated.
= Polarizer scale
Reading to 5°
Swing-out knob for top lens
Polarizer rotation ring
Condenser N.A. is 0.9
(when top lens swings
out N.A, is 0.25)
Filter mount
Accepts 45mm-diam.
ilters.
Bulb mount
Lamp
house clamping knob
The lamp house cover can be
opened by pulling down the
knob; or closed by pushing it up
until it snaps in place.
Before pushing, ascertain that the
knob is positioned as shown in
the picture right, marked with
x O

-
Cut
Here
-
Summary of Putting the Microscope in Operation
A. Match the line voltage selector switch to local mains voltage (see page 5).
B. Switch on the light source.
C. Adjust the trimmer screw until the bulb is dimly lit (page 7).
D. © Place a specimen slide on the stage.
E. Remove the Bertrand lens and analyzer from the light path.
F. Coarse focus with the 10X objective.
G. Make interpupillary and diopter adjustments (page 7).
H. Center the stage (page 9).
|. Center objectives other than 10X (page 9).
J. Swing in the desired objective.
Set the condenser, analyzer and Bertrand lens correctly according to your microscopic
purpose (page 10 and 11).
L. Adjust light intensity
Fine focus.
N. Adjust aperture iris diaphragm (page 9).
Adjustment of Illumination System
Microscopic 5 Bertrand Condenser
Objective
method lens top lens
Orthoscople | 4x to 10x OUT OUT
observation
Conoscopic | 20x to 100X IN IN
observation
For biological use, however, remove the analyzer, Bertrand lens and sensitive tint plates.
* Cut off this page at dotted line and put it on the wall near the microscope for use as a re-
minder of microscopic procedure.
OLYMPUS

V. OPERATION
1. Adjustment of Minimum Line Voltage
The minimum voltage required for the light source can be adjusted with the rheostat trim-
mer screw at the microscope base plate in accordance with the line voltage and frequency.
The built-in rheostat incorporates a thyristor in its semi-conductor circuit for the following
advantages:
(a) Extremely fine adjustment of light intensity can be easily achieved.
(b) Flickering of the bulb filament is eliminated and the light intensity is stabilized.
(c) Increased life expectancy of the bulb.
For adjustment of the minimum line voltage, ascertain
that the voltage selector switch is set to conform with
the local mains voltage, and the sliding control lever 2)
is positioned closest to you (low voltage), and then
activate the main switch @). If the bulb is dimly lit,
the secondary voltage is correct. If it is not lit at all,
rotate the rheostat trimmer screw @) gradually with a
coin, until the bulb is dimly lit; then push the sliding
control lever forward in order to obtain optimum light
intensity. (Fig. 1)
Fig. 1
2. Interpupillary Distance and Diopter Adjustments
1) Insert the eyepiece @) with cross hairs into the right
eyepiece tube of the binocular tube, aligning the ©
positioning slot @) and positioning pin ©). (Fig. 2)
* When the eyepiece positioning pin is inserted into the
lower slot on the tube, the cross lines in the eyepiece
coincide with the vibration direction of polarizer and
analyzer at O“ settings. When inserted into the other
slot, the cross lines are at 45° to the direction of
vibration. (This is the same with the monocular
tube.) Fig.2
Then insert the other eyepiece into the left tube.