Olympus BX53M User manual

Optical Microscope and Accessory
Instructions
This instruction manual is for the system microscope model BX53M.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with
the use of this system, we recommend that you study this manual thoroughly before
operating this system, and always keep this manual at hand when operating this system.
Retain this instruction manual in an easily accessible place near the work desk for future
reference.
For details of products included in the configuration of this system, see page 9.
System Microscope
BX53M

In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.
NOTE: This product has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the product is operated in a commercial environment. This product
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the
user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the product.
This product is applied with the requirements of standard IEC/EN61326-1 concerning
electromagnetic compatibility.
- Emission Class A, applied to industrial environment requirements.
- Immunity Applied to industrial environment requirements.
Some interference may occur if this product is used in domestic location.
For Korea only
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Introduction....................................................................................................................................................... 1
Safety precautions...................................................................................................................................... 2
1 Nomenclature of units........................................................................................................................ 8
2 List of combinable units ................................................................................................................... 9
3 Basic operations of the microscope (brightfield observation).....................14
3-1 Reflected light brightfield observation procedures...........................................................14
3-2 Turning ON the main switch..................................................................................................................16
3-3 Selecting the illumination.........................................................................................................................16
1Changing between the reflected light illumination and the transmitted light
illumination.......................................................................................................................................................................................16
3-4 Selecting the observation method ..................................................................................................17
1When BX3M-RLAS-S is combined.............................................................................................................................. 17
2When BX3M-KMA-S is combined................................................................................................................................ 17
3When BX3M-RLA-S is combined ................................................................................................................................. 17
4When BX3M-URAS-S is combined.............................................................................................................................18
3-5 Changing between the eyepiece light path and the camera light path ............19
3-6 Placing a sample on the stage...........................................................................................................19
1Placing a sample........................................................................................................................................................................ 19
2Adjusting the stage height.................................................................................................................................................20
3Using the Y-axis lock .............................................................................................................................................................21
4Adjusting the tension of the X-axis knob and the Y-axis knob................................................................ 21
3-7 Selecting the objective..............................................................................................................................23
3-8 Focusing................................................................................................................................................................. 24
1Moving the stage vertically................................................................................................................................................24
2Replacing the fine focusing knob ...............................................................................................................................24
3Adjusting the tension of the coarse focusing knob....................................................................................24
4Setting the movement limit with the coarse focusing knob.................................................................25
Contents

5Method to find the approximate focal point easily......................................................................................25
3-9 Adjusting the brightness ...........................................................................................................................26
1When the LED lamp housing is combined.........................................................................................................26
2When the halogen lamp housing is combined ..............................................................................................26
3When the mercury lamp housing is combined...............................................................................................26
4When the light source is combined..........................................................................................................................26
5Using the LIM/SET switch ..................................................................................................................................................27
3-10 Adjusting the observation tube......................................................................................................... 31
1Tilting adjustment ......................................................................................................................................................................31
2Using the eye shades............................................................................................................................................................31
3Adjusting the interpupillary distance........................................................................................................................32
4Adjusting the diopter...............................................................................................................................................................32
3-11 Adjusting the field diaphragm of the reflected light illumination........................34
1Using the field diaphragm (FS) ......................................................................................................................................34
2Adjusting during observation ..........................................................................................................................................35
3Centering of the field diaphragm (FS)......................................................................................................................36
3-12 Adjusting the aperture diaphragm of the reflected light illumination............. 38
1Using the aperture diaphragm (AS)...........................................................................................................................38
2Adjusting during observation ..........................................................................................................................................39
3Centering of the aperture diaphragm (AS)..........................................................................................................39
3-13 Centering the mercury burner............................................................................................................ 40
3-14 Inserting the reflected light illumination filter........................................................................42
1Using the ND filter lever........................................................................................................................................................42
2Using the filter...............................................................................................................................................................................43
3-15 Transmitted light brightfield observation procedures.................................................46
3-16 Adjusting the field diaphragm of the transmitted light illumination.................48
1Using the field diaphragm (FS) ......................................................................................................................................48
2Centering of the field diaphragm (FS)......................................................................................................................48

3-17 Inserting the transmitted light illumination filter.................................................................50
4 Various observation methods...................................................................................................52
4-1 Reflected light darkfield observation procedures.............................................................52
4-2 Reflected light simple polarization observation procedures ..................................53
4-3 Reflected light differential interference contrast (DIC) observation
procedures.............................................................................................................................................................................54
4-4 Reflected light fluorescence observation procedures..................................................55
4-5 Reflected light infrared observation procedures................................................................ 56
4-6 Reflected light simultaneous observation for BF/DF......................................................57
4-7 Transmitted light simple polarization observation procedures............................. 58
4-8 Transmitted light polarization observation procedures ...............................................59
4-9 Setting the analyzer and the polarizer of the reflected light illumination..........60
1When BX3M-URAS-S, BX3M-RLA-S or BX3M-KMA-S is combined...............................................60
2When BX3M-RLAS-S is combined..............................................................................................................................61
4-10 Inserting the DIC slider.............................................................................................................................62
1Inserting the DIC slider..........................................................................................................................................................62
2Adjusting the prism ..................................................................................................................................................................63
4-11 Opening/Closing the shutter ..............................................................................................................64
4-12 Setting the analyzer and the polarizer of the transmitted light
illumination....................................................................................................................................................... 65
1Setting the analyzer and the polarizer....................................................................................................................65
4-13 Inserting the MIX slider for reflected light observation................................................66
1Inserting the MIX slider for reflected light observation..............................................................................................66
2Turning ON the illumination of the MIX slider for reflected light observation.......................66
3Adjusting the brightness......................................................................................................................................................67
4Selecting the illumination pattern................................................................................................................................68
4-14 Using the oil immersion objective.................................................................................................. 69

5 Troubleshooting.................................................................................................................................... 70
5-1 Optical systems............................................................................................................................................... 70
5-2 Electrical systems...........................................................................................................................................74
5-3 Coarse/fine focusing ..................................................................................................................................84
5-4 Observation tube............................................................................................................................................84
5-5 Stage.........................................................................................................................................................................85
6 Specifications..........................................................................................................................................86
7 Optical performance list <<UIS2 series>> ..........................................................................89
8 Assembly.....................................................................................................................................................93
8-1 Assembly diagram ........................................................................................................................................93
8-2 Assembly procedures................................................................................................................................94
8-3 Connection with PC...................................................................................................................................121
9 Preventive inspection sheet for illumination devices .......................................123
10 Proper selection of the power supply cord ...........................................................124

1
BX53M
Introduction
This microscope employs the UIS2 (UIS) optical systems. If you use eyepiece, objective, observation tube or
condenser, etc. together with this microscope, be sure that they apply to the UIS2 (UIS) optical system series.
Using inappropriate units restricts the performance.
(There are units usable with the BX series. Contact Olympus or refer to the latest version of catalogs.)
Configuration of instruction manuals
Read all the instruction manuals provided with the units you purchased.
The following instruction manuals are prepared for the units to be used with this system.
Instruction manuals Main contents
BX53M system microscope
(this instruction manual)
Reflected light brightfield observation, reflected light darkfield
observation, reflected light simple polarization observation, reflected
light differential interference contrast observation, reflected light
fluorescence observation, reflected light infrared observation, transmitted
light brightfield observation and transmitted light simple polarization
observation
BX53/53M-P polarizing microscope Transmitted light polarization observation
BX3M-CB/CBFM
Control box/FM Control box Control box (BX3M-CB)
U-LGPS
LED and LDP light source
Connection of the light guide illumination system with the liquid light
guide, etc.
U-RFL-T power supply for mercury
burner /
U-RX-T power supply for xenon burner
Connection of the mercury lamp housing with the power supply
TH4 halogen lamp power supply unit Connection of the halogen lamp housing with the power supply
Label of the immersion oil
Read the label of the immersion oil you purchased.
Immersion oil Major contents
IMMOIL-8CC
IMMOIL-500CC
IMMOIL-F30CC
Contains the cautions and handling methods of the immersion oil.
Intended use
This product has been designed to observe magnified images of specimens in industrial applications.
Appropriate specimens can include semiconductors, electrical components, molded articles, or mechanical parts.
Industrial applications include observation, inspection or measurements.
Do not use this product for any purpose other than its intended use.

2
CAUTION - Carrying the microscope
CAUTION - Installation of the product -
Safety precautions
Hold the arm of the microscope.
When moving the microscope, hold the arm. (Approx. 15 kg)
Holding the stage, lamp housing or observation tube, etc., may
damage the microscope.
Remove the sample and the eyepiece.
When carrying the microscope, be sure to remove the sample and the
eyepieces to prevent them from dropping.
Also, remove units attached. If you carry the microscope attached with
units, the danger to drop the microscope will be increased due to the
heavier weight.
Do not slide on the top surface of the table.
Do not move the microscope by sliding on the top surface of the table.
The rubber legs may be damaged.
Install the microscope on a sturdy, level table or bench.
If you install the microscope on an unstable table or a tilted table, the microscope may drop and you may get injured.
Prevent the microscope from overturning.
The microscope becomes unstable if the height of the microscope is increased by the accessories mounted on it. In
this case, as this microscope may be overturned, take the actions to prevent the microscope from overturning.
In particular, if following units are combined, the microscope may be overturned even by the tilt of 10° or less.
· Height adapter (BX3M-ARMAD)
· Super widefield erect image tilting trinocular observation tube (U-SWETTR-5)
· Tilting trinocular tube (U-TTR-2)
· Intermediate attachment (U-DP, U-DP1XC, U-ECA, U-CA, U-EPA2, U-CPA, U-OPA, U-TRU, U-KPA)
For measures to prevent overturning, see "Attaching the overturning prevention plate" (page 94).
If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
product may also be damaged. Always use the product according to this instruction manual.
The following symbols are used in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
NOTE
: Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the product or other property.
TIP
: Indicates the useful knowledge or information for use.

3
BX53M
CAUTION - Electric safety -
Always use the power cord provided by Olympus.
If the proper AC adapter, the power cord and other cables are not used, the electric safety and the EMC (Electro-
Magnetic Compatibility) performance of the product cannot be assured. If no power cord is provided, please select the
proper power cord by referring to the section “Proper selection of the power cord” at the end of this instruction manual.
Always connect the ground terminal.
Connect the ground terminal of the power cord and that of the power outlet. If the product is not grounded, our
intended electric safety and EMC performance of the product can not be assured.
Do not use the product in close proximity to the sources of strong electromagnetic radiation.
Proper operation may be interfered. The electromagnetic environment should be evaluated prior to operation of the
product.
Remove the power cord in case of emergency.
In case of emergency, be sure to unplug the power cord from the power cord connector on the product or from the
wall power outlet.
Install the product at the location where you can reach the power cord connector or the wall power outlet at hand to
remove the power cord quickly.
CAUTION - Protection for electric shock -
Keep the power cord and cables sufficiently away from the lamp housing.
If the power cord and cables contact a hot area of the lamp housing, they could melt and cause electric shock.
Do not insert any tools or metal fragments in the air vents of the product.
Doing so could cause electric shock, ignition or failure of the product.
Do not touch the product with a wet hand.
In particular, if you touch the main switch of the power unit or the power cord with a wet hand, electric shock, ignition
or failure of the product may be caused.

4
CAUTION - Protection for injury -
Do not pull the cable with an excessive force.
If you pull the cable connected to the control box (BX3M-CB) with an excessive force, the control box may drop and
your hands may be pinched.
Be careful not to drop the glass plate.
If you are using the glass plate, be careful not to drop the glass plate. If the glass plate drops and is broken, you may
get injured by the broken pieces.
CAUTION - LED (light emitting diode) -
Do not look directly at the light from the LED light source for a long
time.
The LED built in this product is basically eye-safe. However, do not look
directly at the light from the LED lamp house for a long time, since it may
cause damage to your eyes.
If the laws or regulations for workers health and safety have been established,
follow them when using the microscope.
Do not look directly at the light coming out from the objective or the
specular reflection light from the specimen.
Do not look directly at the light coming out from the objective for a long time,
since it may cause damage to your eyes.
Do not expose your skin to the light coming out from the objective
for a long time.
If your skin is exposed to the light coming out from the objective for a long
time, you may get burned.
Do not press the micro switch of the MIX slider for reflected light
observation (U-MIXR) with your finger.
If you press the micro switch aof the MIX slider for reflected light
observation with your finger, the MIX slider for reflected light observation may
emit the light.
Do not insert the MIX slider for reflected light observation (U-MIXR)
upside down.
Do not insert the MIX slider for reflected light observation upside down.
Otherwise, the light coming out from the MIX slider for reflected light
observation enters your eyes through the eyepiece and your eyes may be
damaged.
aDo not press with your finger.