Olympus CHS User manual

MODELSCHS/CHT
BIOLOGICAL MICROSCOPES
scanned by J. G. McHone, 15 Feb 2009
for personal information, not for sale
,------WARNING ------,
11tis i,lUflIf;riof! nwowI is ~ ..- of the Olympul8iologicM
M.accClpft ModM CHS a CHT. W.'lICOI,.,..n you rt«J
Mil 'fUN. t:Mr1Mfy ;" Of'd« to famil;'i" ywrw/I fully
WI,Il ~ _ of l"OUf ~ so dull you un ()brN!
oprNtNm ptfffrxmMPCr.
OLYMPUS'

BEFORE USE
Observe the following procedures carefully:
a Operation_~ ~ _
CD Since the microscope is aprecision instrument, always handle it with care, and avoid abrupt motions or
shocks.
(:i) Avoid exposure to direct sunlight, high temperature and humidity. dust and vibration.
C~) Before bulb or fuse replacement, unplug the power cord from the AC outlet.
(~} AIways ground t he microscope to preven telectric hazard.
® Only use the tension adjustment ring for altering the tension of the coarse adjustment knobs.
@) Be careful not to soil lens surfaces with dust, fingerprints, etc.
Q) Be certain the voltage selector switch on the base plate of the microscope is set to conform with the local
line voltage before use. (CHS only)
*Specifications of the electrical components differ from others than the equipment with 120 V of supply
circuit, as the equipment is in compliance with the requirements of Underwriters Laboratories. Also, the
equipment
is not needed to select voltage,
fJ Maintenance and Storage
(I) Use aclean brush or lens tissue paper to clean the lens surfaces. If the lens surfaces are soiled with oil or
fingerprints, wipe them
off carefully with gauze moistened with asmall amount of acleaning medium
(alcohol and ether 3:7), or xylene.
(2) Do not use organic solutions (e.g. thinner, xylene, ether, alcohol) to wipe painted surfaces or plastic parts
of various components. They should be cleaned with aneutral detergent.
C~) Never disassemble each componen t of the microscope for repair yourself. since the integrated performance
may
be impaired.
CiJ When not in use, the microscope should be covered with the dust cover provided or contained in astorage
case, and kept in aplace free
from humidity and mold.
,.---------------- ,--
Flat washer
Screw
~---
Inslal\ two blocks at Ihe bottom of the wooden case
in the following procedure:
1} Insert one of the two screws into aflat washer and
one
of the two holes (8 mm dia.) as illustrated at
the right.
2) Insert the screw into the block from below, and
clamp
with the spanner provided.
3) Clamp the other block wi th the other screw On the
opp~i te side in the same rnanner as mentioned
above.

CONTENTS
1.
STANDARD CONFIGURATIONS I
2.
SPECIFICATIONS 22
3. NOMENCLATURE 33
4. ASSEMBLY 54
14-1Assembly Diagram
5]
14-2Explanation
for Assembly Procedure
-D
5.
OBSE RVA TION (Putting the Microscope in Operation) 9
DSwitching on the Bulb 9
fI
Spacimen Placement 9
II
Focus
10
..
Inter~uQillary Distance Adjustment
10
II
Diopter Adjustment
11
II
Centration of the Field Iris Diaphragm CH2·FS (optional)
11
II
Objective Change
11
II Adjustment
of the Aperture Iris Diaphragm 12
II
Adjustment of the Field Iris Diaphragm (for CH2·FS onlyt
12
1m
Tension Adjustment of the Coarse Adjustment Knobs 12
II
Locki~ of the Pre-focusing Lever
13
m
Use of Immersion Objectives 13
6. PHOTOMICROGRAPHY 14 6
! 6-1
System Diagram of Photomicrographic Equipment (optionally available) 14 ]
16-2Setup
of the PhotQ'micrographiJc Equipment
iU
[e-3
CQlor Temperature
egula~lor1 ~for use of dayl,ight tv,pe c,olor film}' !D
7.
OPTICAL DATA
16 "
8. TROUBLESHOOTING GUIDE 17 8

Component CI4S;2.3E
CHT·213E
Microscope stand
... vith
quadruple revolving nosepiece,
square plain stage, low voltage illuminator base, including CHS-F 0
dust plug AA7808, filter 32.5C-2, immersion oil 8cc anD
dust cover C011
Microscope stand
with quadruple revolving nosepiece,
square plain stage, 3m"!
illuminator base,
incIuding dust CHT-F 0
plug AA7808, filter 32.5C-2, immersion oil 8cc and dust
cover C011
Power cord
UYCP
00
--
Binoculm observation tube, inclined 45°
CH-BI45-W 0 0
Attachable mechanical stage
with
right-hand
low
drive
CH-MVR
00
controls
Condenser CH2-CD 0 0
Filter holder CH2-FH 00
6V 20W halogen bulb, 2 pes.
6V 20WHAL
0
30W tungsten bulb, 2 pes.
30WSB 0
--
E D achromatic objective
4X ED4X 00
E D achromatic objective
lOX ED10X
00
E D achromalic objective 40X (spring)
ED40X/R 00
E D achromatic objective 100X (spring,
oil)
ED100X/RO 00
LB eyepiece
lOX, 2 pcs. CWHK lOX
0 0
--
tn
Z
o
f-
<1:
a:
:::)
(:J
u..
Z
o
u
o
a:
<1:
o
z
<1:
f-
tn
I
,STAN.DARD, CONFIGU'RAT'ONS~~ , , ~ - .
_r _ _. _ _ ~
Note: 0indicates the compatible components for each model.
Optional accessories:
Field iris diaphragm attachment
Phase contrast attachment
Simple phase contrast attachment
Simple polarizing attach
men t
Dual viewing
attachment
Magnification changer
Vertical
illuminator
Dry darkfield condenser
Immersion darkfield condenser
Wooden storage
case
CH2-FS
CH2-PCD-PL
CH2-PCPL
CH2-POL SET
CH2-DO
BH2-CA
BH2-KMA
SH-DCD
BH-DCW
CHS-/CHT-WB
D--------------------------

2
SPIC II FI CATIO NS
litem
~
Description
M~crosoope
I[;ircurar QQvetail
mount lor observation tube wi th accommodation
to ac·
cept an analyzer-; built-on quadruple nosepiece and pla'lIl stage 124rnm(XI
limb
x153 rnm (V}
Coaxial coarse and fine focusing controls within afocus adjustment range
Focus of
25mm. Fine adjustment knobs graduated in increments 01
2.5/1.
adjustments Tension adjustment ring for coarse adjustment knobs and pre-focusing
Microscope lever for coarse focusing.
stand Condenser holder
Rack and pinion condenser height displacement up to 28mm.
Illuminators built-in bases:
6V 20W halogen bulb with transformer built-in CHS-F
T
lOV!120V 30W tungsten bulb with reflector built-in CHT-F
Base
Ligtll intensity variable by means of coil winding resistance
Mount at the light exit on the base for field iris diaphragm and 45 mm-dia.
filter.
Power cord is detachable.
Observation Inclined 45°;
interpupillary distance adjustment with a
scale between
Binocular 53 mrn and
72 mrn.
tube Left-side eyepiece tube equipped
with diopter adjustment ring.
Mechanical stage Low-positioned coaxial
control knobs; X-V traversing
area 76mm
x
50 rnm.<€ompatible with two standard slides simultaneously.
N.A. 1.25 (in immersion
oill. wi th graduated aperture diaphragm. Pro-
Condenser vided
with accommodation to accept a filter holder and an attachment
Jens for field iris diaphragm.
-------
FiItel' holder Accepts a 32.5 mm dia. filter.
Field iris Iris diaphragm Attachable on the light
exit mount and accepts a45 mm dia. filter. Dia-
diaphragm frame phragm image
con be formed in conjunction with obj'lctives frorn lOX to
attachment 40X.
(optionally
Attachable at the lower end of the condenser and accepts a32.5 mm dia.
available)
Attachment lens filter; provided
with centering screws for iris diaphragm.
Filter
Blue filter (325 mOl dia.)
Objectives
ED4X. EDlOX, ED40X (spring-loaded), and
ED100X (spring-loaded, oil
immersion)
Eyepiece ICWHKTOX. Field No. 18; compatible
with an eyepiece micrometer.
I
Dimensions 180 mm (W) x223 mm (OJ x392 mm (H) (binocular version)
-
Eyepoint height
391 mm
Weight CHS-213E: 6.2
kg (13.7Ibl
CHT-2T3E: 5.8 kg
(12.8Ibl
Power consumption (maximum)
CHS: 32 VA CHT: 32 VA
-----------------------------B
II
en
"'0
m
o
o
~
o
Z
en

3
"N OMEN CLATURE ' .. -. ' " - _. - .
- , - I _ _ '
Interpupillary distance scale.
w
a:
::>
t-
e!
...J
U
Z
w
::;E
o
z
[Eyepiece I
Diopter adjustment ring
[ReVolving nosepiece I
I Object ive I
1 Mechanica~
[ Base I
=------
Power switch
I Binocular tube]
Observation tube clamping screw
[Microscope standJ
Specimen holder
Tension adjustment ring
~
,/ Coarse adjustrnent knob
Fine adjustment knob
--Graduated in ir.crernenls of 2.5/-1.
(fuwer ,;,ord I
Voltage control dial
Field iris diaphragm attachment CH2-FS (optionally available)
(CHS-F)
Line voltage selector switch
Lamp housing cover
Diaphragm ring
!Iris diaphragm frame
/
IAttachment lens I
./
Centering screw
Accepts d115mm·dia. filler.
Filter mount
11------------------------------

Pre·focusing lever
Fuse holder
Power cord receptacle
Condenser height adjustment
knob
Bulb socket (CHS-F)
For 6V 20W halogen bulb.
Bulb socket (CHT·F)
For 110V!120V 30W tungsten
bulb.
Condenser clamping $1:reW
Aperture iris
diar;lnrag lever
Filter holder
Accepts a 32.5mrn dia filrer.
ICondan:;ar I
Filter mount
Accepts a 45rnrn-dia. filter.
Lamp housing knob
The lamp housing cover can be
opened by pulling down theknob;
or clo:red by pushing it up unt il it
snaps in place.
8efore pushing (he lamp housing cover. be
cerWin the knob is positioned as shown in the
picture lef1 (marked wi th a circle).
II
z
o
5::
m
z
(')
r
»
--l
c
:0
m
---------------------------B

ASSEMBLY ~. . ' . -- -'. . .' ~ .
-- -
Assembly Diagram
*Assemble each component in the order of the numbers wi th care to keep all glass surfaces clean and avo'ld
scratching
the lens surfaces.
'Opt"lonal accessories
___ ---------111
Filter
(32.5mm dia.!
-'f.-:::J=
Power cord
II!J
•
n
110 V1120 V 30W
tungsten bulb (for CHT)
Observation tube
II
II
I
~
D
Condenser
Stage
clip
Objective
Mechanical stage
I'I! ""
At tachrr.ent
lens·
1
c==J
Filter
holder
6V 20W
halogen bulb
(for CHSl
Field iris diaphragm" ~

-- • Iii· •. I ~ ~ - • ~ ~ i .; .... -:. .. ;.-; - -
• ~ ~ • 4
Explanation for Assembly Procedure
csewng of the line voltage selector switch in position (for CHS only)
Ascertain that the line voltage selector switch CD is set in conlormity
with the local line voltage. If not. the switch should be set at 100V
(for 110V to 120V) or 200V (for 2.20V to 240V) correctly by means
of ascrewdriver. (Fig. 1)
* For the U.S.;
Frames sold in the U.S. meet the UL standard and are set at
115V. Therefor. this selector switch is not included.
Fig. I
II
oBulb installation and re 'acement
1) Turn the l'licroscope on its side and pull the lamp housing knob ~r to
open the lamp housing cover (;2). (Fig. 2)
2} Install the
bulb .
•
6V 20W halogen bulb (for CHS);
Hold the halogen
bulb contained in a polyethylene bag to avoid leaving
fingerprints on the
bulb, and insert the contact pins into .the bulb
socket (3; all the way. (Fig. 2)
Fig. 2
•110 V/120V 30W tungsten bulb (for CHT);
Insert the tungsten bulb with its mirror portion '3) located at the
lower side
into the bulb socket @; then. pressing it against the socket.
rotale the bulb clockwise. {Fig. 3)
*Before use, wipe off fingerprints or soils on the bulb.
*110 V bulb for local line voltage 110 V, and 120V bulb for local line
voltage 120 V
or higher.
3) After bulb installation, close the tamp housing cover CV. pUshing in the
cover
knob 0), (Fig. 3)
* If the bulb burns out during observation, be certain to cool the
defective
bulb completely before replacement.
Fig. 3
II .Mounting the observation tube
1) Loosen the clamping screw CD fully, and mount the observation tube
on the stand. Reclamp the screvv CD to securely hold the observation
tube
on the stand. (Fig. 4)
2)
The binocular tube is normally localed in the direction of the micro-
scope Iront, but it can be turned to any other direction, if necessary.
II Eye ieee insertion
Insert the eyepieces into the eyepiece tubes (2). (Fig. 4)
Fig. 4
-----------------------------11

{-IU5e
of an eyepiece micrometer (19 mm in diameter) I
An eyepiece micrometer (10 ITlm/lCO) (optionally available) can be
inserted
into the eyepiece CWHK10X in the following procedure:
1) Remove the retaining ring (I) from the 10\o"ler end of the eyepiece i
and place the micrometer on the retaining ring with the reticle-·
engraved surface, facing
downward. (Fig. 5) ?
* Be certain to clean the micrometer disc before inserting into the
eyepiece.
2) Return the retaining ring inlO the eyepiece and insert the eyepiece'
into the eyepiece tube. _
._.A~""'_~ ," __ '.
Fig. 5
II Mounting the objectives
l} Lower the stage by means of the coarse adjustment knobs.
2} Screw the objectives into the nosepiece, from low power to higher
power in aclockwise direction. (Fig. 6)
*Close the empty aperture in the nosepiece with aplug (I) provided.
(Fig.
6)
Fig. 6
II Mounting the mechanical s ... ta..,9 .... e _
Place the mechanical stage on the plain stage 0), with the specimen
traversing guide closest to the microscope pillar, and tighten the
stage clamping knobs (2; with a coin (Fig.7}
•Insertion of stage clips
The plain stage is pre-drilled for insertion of the stage clips when the
mechanical slClge is not attached (see page 4).
Fig. 7
II Mounting the condenser
Insert the condenser into the condenser holder (2Jfrom below,
with the condenser iris diaphragm lever, pointing in the microscope
front. and tighten the clamping sere'.'v (i). (Fig.8)
Fig. 8
B-----------------------------

II Insertion of the blue filter _
Slip the blue filter (32.5 mm dia.l (2; into the filter holder :1: (or
into the filter mount at the top of the 8ltdchmel1t lens). (Fig. 9)
DInsertion of the filter hofder
Insert the filter holder ~1; into the condenser from belovv. (Fig 9j
Fig. 9
Mounting the field iris diaphragm attachment
(optionally available)
11 Aligning the positioning clips of the ins diaphragm frame to the cut-
outs in the tilter mounl (C on the base. insert the iris diaphrcgm
until it clicks in position. (,Fig. 10)
2) Place the blue filter on the attachment lens and in5en the attach-
ment lens into the condenser from belo,;v, with the leiters "OLYM·
PUS" facing in the rnicrocsope fronl.
Fig. 10
II
Fig. 11
Typical example of 3-contact
grounding plug
1m Connecting the power cord
1) Plug the power cord (n into the receptacle (2) on the microscope
base. (Fig. 11)
2) Connect the primary cord I,..,ith a 3-contact plug (it I·vill fit into a
ground tvr-e pO'..ver outlet, and no need to connect it to any other
grounding device) to an AC outlet.
* If a2-<:ontact grounding plug is used, ground the microscope to a
properly grounded device (except a gas pipe), If necessary, use an
extension cord.
*This microscope incorporates anoise filter in the electric circuit
built-in the microscope stand, bleeding a very low voltage current in
order to reduce effect of any external noises. Therefore, if the
conductive part of the stand is touched without grounding, an
electric shock may sometimes be felt depending upon the humidity
conditions of the hands and foot wears.
--------------------------11

•
• F use replacement
1) The fuse box (I;: is located at the back of the microscope base.
([= ig. 13)
2} Disconnect the power cord from the AC outlet. and remove the fuse
box ct from the base by means of ascrew driver. (Fig. 13)
* Apply the tip of the screw driver at the lower edge of the fuse
box to remove it.
*The fuse box can accommodate aspare fuse in it.
Use afuse as designated by the manufacturer:
ivlicroscope F
use a rn per age
Fig.
13
100V
1A
CHS
200V
0.63A
CHT
100V
0.8/-\
200V
O.63A
z
o
~
~
a:
w
V)
en
o5- -
OBSERVATIO N- ~P~t1ing t~el~i~r~5~t?P~ j~ ~PlJra~ion' .
D Switchin~ on the bulb
I) Turn the power 'switch j; ON and adjust the voltage control dial (~;
until ropa! i!\ mity is obt~i ed. (Fig. 1r.)
2) Rotdte the dial (2; toward reoperator (clockwise) to darken inten·
sity (in tile direction of tne arroWl, or reverse the dli:ll (counterc]ock·
'Nlse) to brigh ten
Fig. 14
Fig. 15
fJ Specimen placement
1} Open the spring-loaded finger cD of lhe specimen holder with one
hand, and insert the specimen slide
with the cover glass above the
specimen
into the holder INith the other hand. (Fig. 15)
* Be careful to release the finger CD gently after the specimen is
placed inside the holder.
*Asudden release of the finger may cause damage to the slide. If
fragments of the specimen slide fall on the sliding surfaces of the
stage
or condenser, malfunctions may result.
2) For use of the stage clips in place of the specimen holder, attach a
pair
of stage clips on the stage, and insert the specimen slide between
lhe stage surface and the clips near
the clip stems; then move the
slide toward the stage center.
11---------------------------

•Cover glass
Use cover glasses of 0.17 mm thickness in conjunction with the objectives marked wi th the inscription
"160/0 .17" for op(lmum performance of these objeclfves.
•Specimen slide
Specimen slides between
0.9 mm and 1.2 rnm in thickness are recommended for the CHS/CHT microscopes.
If the thickness of aslide exceeds this range, illumination may sometimes be impaired.
D Focus
1) S",>,ing in the lOX objective.
2) Bring the specimen
into focus by means of the coarse and fine
adjustment knobs.
* Rotate the focus adjustment knobs clockwise (in the direction of
the arrow in Fig. 16), and you can raiS1! the stage (or the specimen
approaches the objective I.
•o
OJ
Ul
m
::0
<
»
-l
o
Z
Fig. 16
III Interpupillarv distance adjustment
1) Looking through the binocular tube, move the knurled dovetail slides
CU in the directions of the arrows until aperfect binocular vision
is obtained. (Fig. 17)
2) If you rr,emorize your interpupillary distance setting on the scale
(2) provided between the dovetail slides CU, it is convenient \0 ob-
tain a
proper selling next tin~e. (Fig. 17)
Fig. 17
-----------------------------------mJ

- - -
r .' ~
I • I ~
,,
..'
... ~ I _ •
Fig. 18
•z
o
f=
;;
0::
UJ
(J)
IXl
o
Fig. 19
Image of the field
iris diaphragm
Field of view
Fig. 20
Fig, 21
III Diopter adjustment
1) Look at the image through the right eyepiece with your right eye, and
focus on the specimen "\lith the focus
adjustnlent knobs.
2) Next, looking at the image through the left eyepiece ','.lith your left
eye, rotate the diopter adjustment ring :5; to focus on the specimen
'Ivithout using the focus
adjustment knobs. (Fig. IS}
II Centration of the field iris diaphragm CH2·F~ (optional)
1) Rotate the diaphragm ring C',) counterclockwise to stop dO'lvn the iris
diaphragm
to the minimum. (Fig. 19)
2) Rotate the condenser height adjustment knob (~; in either direction
until the image of the field diaphragm is viSible sharply in the held of
view. (Fig. 19)
3) Bring the image of the field diaphragm into tile center of the field by
means of the two attachment lens centering screws (3). (Figs. 19,20)
4} Open the diaphragm until the small ring of the diaphragm inscribes the
field of view. If the polygonal ring is not concentric with the field of
viev.;, repeat the centering procedure mentioned above. (Fig. 20)
5)
After centration is complete, re-open the diaphragm until it becomes
alarger
polygonal ring around (or circumscribes) the circular edge of
the field.
II Objective change
I) Swing in the objective to use. (Fig.21}
2) Be certain to click the nosepiece in position.
11-----------------------------

Fie. 22
Exi l pupil
of objectille
(Image of aperture diaphragm as seen
through empty eyepiece tube)
Fig. 23
Field iris diaphragm
J
Fig. 24
Fie. 25
II Adjustment of the aerture iris diaphragm
The opening of the aperture iris diaphragm built in the condenser
can be adjusted to match with the numerical ar::erture of the objec-
tive in
use, in order to achieve optimum objective performance as
depth of focus, image cDlltr'ast and resolution.
1) Turni ng the diaphragm lever CD counterclockwise reduces the d ia-
phragm or::ening. (Fig. 22)
2) Remove the eyepiece, and
looking at the exit pupil of the Objective
through the
empty eyer::iece tube, adjust the opening of the diaphragm.
Generally, iI
is preferable to stop down the aperture diaphragm to 70%
1O 80% of the objective NA (Fig. 23)
1ft he specimen isl igh lIy stained, or al most colorless and tra nsparent,
further reduce the diaphragm or::ening to increase contrast for better
image observation. Be
careful, ho'wever, if the diaphragm is stopped
down too much, the resolution will be deteriorated.
II Adjustment flf the field iril-diaphragm (for 'CH2-F-S only'
') The field iris diaphragm controls the diameter of the ray bundle im-
pinging on the specimen and therefore,
by stopping down the field
diaphragm
until it is slightly larger than the field of vi e'.'J, it can reduce
stray
light, which in turn increases image definition and contrast. (Fig.
24)
2} Turning the field d'laphragm ring CD {Fig. 19} counterclockwise reduces
the diaphragm opening,
or reverse the ring to increase the opening.
1m Tension adjustment of the coarse adjustment knobs
') Atension adjustment ring (J~ is provided next to the coarse adjustment
knob. With this device the tension of the coarse adjustment is freely ad-
justable
for either heavy or light movement, depending upon operator
preference. (Fig. 25)
Applying the tip of alarge screwdriver at agroove in the periphery of
the tension adjustment ring CD, rotate the ring in the direction of the
arrow to increase the tension, or reverse the ring to loosen.
2) However,
do not Icosen the tension adjustment ring too much, because
this
may cause the stage 1O drop or the fine adjustment knobs to slip.
BI
o
!Xl
CIl
m
:0
<
~
-i
o
Z
-----------------------------11

z
o
I-
~
0::
UJ
(/)
Q)
o
Fig. 26
II locking of the pre-focusing lever
This lever (I) is jJrovided to prevent possible conlact between speci,
men arid objeclive as well as to simplifv coarse locusing The le~'€'r is
locked in the direction of the arrow in Fig. 26, after coarse focus has
been cC'o plished, Thi~ l<. convenient for liquid apol cation or
change of specirnens, roo. since it' prevenl5 further upward lravel 01
,he stage bV means oj the coarsl3 adjUst ent knobs, and provides a
I'rnitlng stop if the St;:ly8 is lowered and then raised again. The pTe-
focusing lever does not restrict fin.e foCUsing.
* Unlock this lever when not in use.
If) Use of immersion objectives
1) To utilize the full numerical aperture of an irnmersion objective [wilh
inscription "oil"), the ob~ec;tive alld spEo:cim n i:!re Imr'llSHed in an im-
mersion oil in a lollowing procedure:
2) Focus on the specimen with a low power objective.
3) Put a drop of immersion oil on the sJ:ecimen slide and the front lens
of the immersion objective.
4}
Turn the nosepiece to bring the immersion objeclive inlO the light path,
and focus
'I,-,ith the Hne adjuslment knobs.
*Use of the pre·focusing lever facilitates steps 2) through 4) above.
*Care should be taken to prevent oil bubbles from forming in the oil
film; jf
any. re-apply immersion oil, since these bubbles greatly
deteriorate the lens performance.
* Be careful not to stain other objectives with immersion 'oil, and
after use, carefully wipe off the immersion oil on the objective, etc.
completely.

6
PHOTOMICROGRAPHY ~ .
A trinocular tube BH-TR45W, optionally available, 'IS used for photomicrography with the CHS/CHT
microscope in conjunction wi th Olympus pho tomicrographic equipment PM- I OA K (automatid, PM-10M
(manual), photomicrographic Camera BH2-PM-6-B, OM camera backs, etc. Read the instruction manual of
each equipment in use for explanation in detail.
6-1 System Diagram of Olympus Photomicrographic Equipment (optionally available) ------,
'--------------~
Photo microgra phic
sV$tem PM-10M'
Photomicrographic
camera BH2-PM-6-8'
OM camera back •
Photomicro
adapter MOM-L"
11
Photo
.1 eyepiece ,
- NFK" -----L~
Photo eyepiece adapter
PM-ADF"
Trinocular tube
BH·TR45·W·
'Optional accessories
6-2 Setup of the Photomicrographic Equipment
2) Use the photo eyepiece NFK3.3X or NFK5X.
4) Looking through the focus'lng telescope (2), focus on the specimen.
(Fig.27}
3) Pullout the light path selector lever (J)to deflect the light to the photo
tube, (Fig. 271
"'0
:::r:
o
-l
o
s:
o
:JJ
o
G')
:JJ
~
"'0
:::r:
-<
iii
Mounting the PM-l0AK, PM-10M or PM-6-8 on the
mictosco
1) Each of these attachments can be mounted on the photo tube of the
trinocular tube as photographed in Fig 27. (F ig. 27 shows t he PM·
lOAK on the t(lnocular tube.)
..
Fig, 27
*In case of long time exposure, ambient light in the room will !p through the eyepieces, and its image
formed
on the film plane may cause ghost or flare; to exclude this extraneous light, dim the room or cap
the eyepieces during photographic procedure,

Fig. 28
fJ Mounting the OM camera back
1) Mount the photo ey piece adapter P,'v1·ADF C1; (into which achoice of
NFK photo eyepiece is inserted), Photomicro adapter {MOM-U ~2~ and
OM camera back '3; on the photo tube in this order. (F ig. 28)
2) The compatible photo eyepieces are NFK3.3X and NFK5X.
3) Bring the specimen into focus. looking through the viewfinder of Ihe
camera.
4)
Photomicrographic magnification equals the objective magnification
multiplied by the photo eyepiece magnification.
*To take agood photomicrograph with asingle lens reflex camera back, a shutter speed at 1/2 second or
slower is recommended so as to reduce the mirror·lockup shock.
Use of the light balancing filter 45 LBD-2N
(optionally available)
1) Remove the blue filter from the condenser.
2) Slip a
45LBD-2N filter into the filter mount CG at the light exit on the
microscope base. (F ig. 29}
3) Turn the voltage control dial (2) until the dial is aligned at the gradua·
tion "10" (maximum), and daylight color can be obtained. {For CHS
only)
*The 45LBD-2N filter cannot be used with the CHT microscope for
color temperature regulation.
Fig. 29
6-3 Color Temperature Regulation (for use of dayligbt color film) I
~===::~~~~:--::=--==-:~=----:----=------====~
D
>-
J:
Q..
<:(
cc
t=l
o
cc
o
:2
o
r
o
J:
Q..
The optionally available color balancing
filter 45LBD·2N is sr;ec;all\' designed 10
obtain a beller color rendition for daylight
\Yr;e color film thi:l.n a blue lilter.
D Use of the b~ue filter 32.5C-2
1) Engage the blue filter as used for observation in the condenser.
2) To obtain color temperature for daylight type film, set the voltage
control dial (~) at the graduation "9" for CHS. or at the graduation
"10" for CHT.
* If a photograph tends to overexposure, do not change the voltage control dial, but apply an optional N.D.
filter
(45mm dia.) to the filter mount at the light exit on the microscope base.
*Since the blue filter tends to characteristically leave somewhat ayellowish tint, an optionally available
45 LBD-2N is recommended for better improvement of color rendition in conjunction with the CHS micro-
scope. However, the CHT microscope can be used
only with the blue filter for color temperature com-
pensation.
11----------------------------

7
OPTICAL UATA :
• D Achromat
I
I
IObjective Type o
Achromat
Magnifiction 4X
JOX
40X 100X'
N.A. 0.10 0.25 0.65 1.30
1,11/.0. (mm) 18.23 7.18 0.63 0.20
--
Focal length (mm) 30.03
1690 4.58 166
--
Resolving pO'.ver (p) • • 3,4 1.3 0.52 0.26
Eyepiece Remarks Spring-loaded
-
Total magndicalion 40X
WOX
400X 1000X
CWHK10X Focal depth
(,u)
172.5 27.60 3.03 0.66
(Field number 18) -
Field of view (rnm) 4.5 1.8 0.45 0.18
• E D Achromat
Objective Type
ED Achromat
Magnification
4><
lOX 40X 100X'
N.A. 0.10 0.25 0.65 1.25
W.o. (mm) 29.00 6.30 0.53 0.20
Focal length (mm)
31.05 16.45 4.59 1.90
Rt"$Olving -power (P)'.
3.4
1.3 0.52 0.26
Eyepiece Remarks Spr;ng-loaded
Total magnification 40X 100X 400X
l00<..,X
CWHK10X Focal depth
(,u)
172.5 27.60 3.03 0.67
(Field number 18) Field
of view (mm) 4.5 1.8 0.45 0.18
"Immersion obiectives
"The resolving power is obtained v-lith L1le fully opened aperture diaphragm.
Glossary
Working distance:
Numerical aperture:
The distance
from the specimen or cover glass to the nearest point of the objec-
tive.
The N.A. represents aperformance number
which could be compared to the
relative aperture (f-number) of acamera lens. The quantity of light which the
objective receives from the object increases with the square of the performance
number.
Field number:
Focal
depth:
Resolving power:
The resolving power of alens is measured by its ability to separate tvvo points.
The distance between the upper and lower
limits of sharpness in the image
formed by an optical system. As you stop down the aperture iris diaphragm,
the focal depth becomes deeper. The larger the
N.A. of the objective the
shallower the focal depth.
Anumber that represents the diameter in mm
of the image of the field dia-
phragm that
is formed by lens in front of it.
Field-of-vi8'N diameter: The actual size of the field of view in mm.
II
o
"'0
-l
()
»
r
o
~
»
Total magnifiC<ltion. Equals the objective magnification
multiplied by the eyepiece magnification.
----------------------------111

8- -
TROUBLESHOOTING GUIDE
- ~ --
w
o
::::>
t:)
t:)
z
r-
o
o
I
Cf)
w
~
C1l
::::>
o
a:
r-
If you are unable to obtain full performance from your microscope because of your unfamiliarity, please
consult with tile table below as pointers lor troubleshoot"lng'
"
Troubre
Cause
Remedy
._--
-
1.
Optical system
a) Field
of view is cut off, or Nosepiece is not clicked into place.
Slightly
rotate the nosepiece until it
illuminated irregularly. clicks
into position. {po 11)
Condenser IS not correctly
mounted AB-insert the condenser all
the
'Nay
on the condenser holder,
without t.lt, (p. 7}
Field ids dlaphragm is not centered. Center
it correctly.
(p 11)
FIeld iris dl,mhraglTl is stopped down Open it properly. (p 12)
too much.
Dust or
dirt
on objective, eyepiece, Clean
ch lens or glass.
condenser or light exit glass on micro
scope base
b) Dust or dirt is visible in DU$t on the light
exit glass on the
1-
the field of view. croscope base
--
Remove dust or dirt, or clean the
spe-
DU5t on the condenser top lens
Icimen.
Diny specimen
Dusr on eyepiece
c) Excessive irnilge contrast Condenser
is lowered too much, Raise the condenser. (p. 71
Apenure iri.s diaphragm is stopped Open the diaphragm. (p. 12)
down excessively.
.-
d} Resollllion problems: Objective
is
not correctly engaged in Slightly
rotate the nosepiece
until it
• I mage is not sharp. the light path. clicks
into posi tion. (p. 11)
•
Insufficient contrast
f----
011'1 on the objective front lens Clean the objective.
• I
mage details lack objective
is used Apply immersion oil.
(p.13)
definition.
Irnmersion I,vithout
immersion oil.
Bubbles in the
immer~ior) oil
Remove bubbles. (p. 13)
Olympus im
ersion oil is fiat u~ed.
Use Olympus immersion oil.
Dirty specimen Clean the specimen, eyepiece or con-
Dust
On eyepiece or condenser top lens
denser lens.
e} Field
of view
is
partially
Objective is not correctly positioned in Slightly rotate the nosepiece until it
out of focus. the light path. clicks
into position. (p. 11}
Specimen IS not
correctly placed on Replace
it on the stage correct 1',1 and
the stage. secure
it with the specimen holder or
stage clips.
(p.9)
-
I)
Image is lillted yellowish. 81LtS 111 ter i~ not engaged. Engage blue
filter.
(p.8)
11-----------------------------