Optika Italy B-150 Series User manual

Model
B-150 series (B-151 / B-153 / B-155 / B-157 / B-159)
B-150 ALC series (B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC)
B-150R-PL series (B-151R-PL / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL)
B-150 Series
INSTRUCTION MANUAL
Ver. 12.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-151 / B-151R-PL 4
5.2 B-153 - B-155 - B-157 - B-159 /B-152R-PL -B-153R-PL -B-155R-PL -B-157R-PL -B-159R-PL 5
5.3 B-151ALC - B-153ALC - B-155ALC - B-157ALC - B-159ALC 6
6. Unpacking 7
7. Assembling 7
7.1 B-151 / B-151ALC / B-151R-PL 7
7.2 B-152R-PL / B-153 / B-153ALC / B-153R-PL 8
7.3 B-155 / B-155ALC / B-155R-PL 8
7.4 B-157 / B-157ALC / B-157R-PL 9
7.5 B-159 / B-159ALC / B-159R-PL 9
7.6 Assembling the microscope 10
7.7 Polarizing set (optional) 11
8. Use of the microscope 12
8.1 Light intensity adjustment 12
8.2 Use of ALC system 12
8.3 Coarse focus tension adjustment 12
8.4 Stage 12
8.5 Adjust the interpupillary distance 13
8.6 Diopter adjustment 13
8.7 Use of oil immersion objective 13
8.8 Aperture diaphragm 14
8.9 Use with rechargeable batteries 14
8.10 Use of the polarizer (optional) 14
9. Maintenance 15
10. Troubleshooting 16
Equipment disposal 17
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
STAGE CLIPS
5.1 B-151 / B-151R-PL

Page 5
TENSION
ADJUSTMENT
COLLAR
SLIDE
HOLDER
5.2 B-153 - B-155 - B-157 - B-159 /B-152R-PL -B-153R-PL -B-155R-PL -B-157R-PL -B-159R-PL
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
STAGE
OBSERVATION HEAD
-) MONOCULAR (B-152 / B-153 / B-155)
-) BINOCULAR (B-157 / B-159)
CONDENSER
OBJECTIVES
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
NOSEPIECE
X/Y MOVEMENT
KNOBS
EYEPIECE

Page 6
5.3 B-151ALC - B-153ALC - B-155ALC - B-157ALC - B-159ALC
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
ALC ON/OFF BUTTON
ALC CONNECTION CABLE

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-151 / B-151ALC / B-151R-PL
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
③
④
⑤
⑥⑦
⑧
①
②

Page 8
7.2 B-152R-PL / B-153 / B-153ALC / B-153R-PL
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives
B-152R-PL (4X / 10X / 40X)
B-153 (all) (4X / 10X / 40X / 60X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil
③
④
⑤
⑥⑦
⑧
①
②
③
④
⑤
⑥⑦
⑧
①
②
⑨
7.3 B-155 / B-155ALC / B-155R-PL

Page 9
7.4 B-157 / B-157ALC / B-157R-PL
① Frame
② Binocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil
① Frame
② Binocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X / 60X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
7.5 B-159 / B-159ALC / B-159R-PL
③
④
⑤
⑥⑦
①
②⑧
③
④
⑤
⑥⑦
⑧
①
②
⑨

Page 10
7.6 Assembling the microscope
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
Only for ALC models:
3. Connect the cable of the ALC (Automatic Light
Control) system to the socket placed on the
observation head and on the back side of the fra-
me. (Fig. 2)
4. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• For models B-151, B-152, B-153 and B-155:
only one eyepiece is used as these models
are monocular.
5. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Page 11
7.7 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the base
of the microscope. (Fig. 5)
2. Loosen the head xing knob ② and remove the
head from the microscope frame. (Fig. 6)
3. Insert the analyzer ③ into the hole inside the fra-
me. (Fig. 7)
4. Put back the head into its original position and
lock the xing knob.
Fig. 5
Fig. 6
Fig. 7
①
②
③

Page 12
8. Use of the microscope
8.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to
turn ON / OFF the microscope and to increase / de-
crease the illumination voltage. (Fig. 8)
8.2 Use of ALC system
1. Press the ALC button ② (Fig. 9). The light on the
microscope will turn o󰀨 for some seconds, the will
turn on again.
2. An optimal brightness level is set
3. Now the system will automatically adapt the
brightness to the eyepieces when an objective is
changed, when the aperture diaphragm is used
or when another specimen is placed on the stage.
4. Pressing the ALC button again, the ALC system
will be disabled.
• When ALC system is active the light intensity
dial is not active.
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
1. To modify the tension according to personal’s
needs, rotate the ring ③ using the provided tool
(Fig. 10).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension.
8.4 Stage
All models except B-151 series
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 11)
1. Open the spring arm of the slide holder ④ and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cau-
se the falling of the slide.
①
②
Fig. 8
Fig. 9
Fig. 10
Fig. 11
④
③

Page 13
8.5 Adjust the interpupillary distance
• This operation can be done only on binocular
models.
1. Hold the right and left parts of the observation
head using both hands and adjust the interpupil-
lary distance by turning the two parts until one
circle of light can be seen. (Fig. 12)
• The graduation on the interpupillary distance in-
dicator ①, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.
8.6 Diopter adjustment
• This operation can be done only on binocular
models.
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ②to compensate. (Fig. 13)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s dio-
ptric correction.
8.7 Use of oil immersion objective
B-155 and B-159 models only
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the spe-
cimen to be observed. (Fig. 14)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, ful-
ly open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be circular
and bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles to
move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft pa-
per towel or a lightly moistened optic paper with
a mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
men would be di󰀩cult (even not impossible)
due to the presence of an additional thickness
on the objective.
Fig. 12
②
①
Fig. 13
Fig. 14

Page 14
8.8 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aper-
ture diaphragm a󰀨ects the image contrast. Incre-
asing or reducing this value one can vary reso-
lution, contrast and depth of focus of the image.
Move the diaphragm lever ① (Fig. 15) toward left
or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, lo-
oking into empty sleeve, adjust the condenser’s
diaphragm in order to obtain an image like the
one in Fig. 16.
8.9 Use with rechargeable batteries
This operation can be done only with “R” models.
When the microscope is plugged with the power sup-
ply, the LED indicator for the battery recharge ②is
lit. (Fig. 17)
• LED red: battery under charge
• LED green: battery fully charged.
When the microscope is unplugged, the LED is o󰀨.
During the normal use with batteries, LED is always
OFF.
8.10 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer
until the darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Cros-
sed Nicol” position) it is possible to begin the
observation.
Fig. 15
①
Fig. 16
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 17
②

Page 15
9. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover,
and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in o󰀨-
position.
• Users should observe all safety regulations of the region. The equipment has acquired the
CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near
sparks or near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 16
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries are uncharged Fully charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section:
The LED doesn’t turn on. No power supply Check the power cord connection
Batteries are uncharged Fully charge the batteries
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube:
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation. Upon looking into eyepieces, try look- ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.

Page 17
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the
reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be col-
lected separately from other waste. The separate collection of this equipment at the end of its lifetime is organized
and managed by the producer. The user will have to contact the manufacturer and follow the rules that he adopted
for end-of-life equipment collection. The collection of the equipment for recycling, treatment and environmentally
compatible disposal, helps to prevent possible adverse e󰀨ects on the environment and health and promotes reuse
and/or recycling of materials of the equipment. Improper disposal of the product involves the application of admini-
strative penalties as provided by the laws in force.

®
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®
OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
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Modelli
Serie B-150 (B-151 / B-153 / B-155 / B-157 / B-159)
Serie B-150 ALC (B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC)
Serie B-150R-PL (B-151R-PL / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL)
Serie B-150
MANUALE DI ISTRUZIONI
Ver. 12.0 2019

Pagina 20
1. Avvertenza 21
2. Simboli 21
3. Informazioni sulla sicurezza 21
4. Utilizzo previsto 21
5. Descrizione dello strumento 22
5.1 B-151 / B-151R-PL 22
5.2 B-153 - B-155 - B-157 - B-159 / B-152R-PL -B-153R-PL -B-155R-PL -B-157R-PL -B-159R-PL 23
5.3 B-151ALC - B-153ALC - B-155ALC - B-157ALC - B-159ALC 24
6. Disimballaggio 25
7. Assemblaggio 25
7.1 B-151 / B-151ALC / B-151R-PL 25
7.2 B-152R-PL / B-153 / B-153ALC / B-153R-PL 26
7.3 B-155 / B-155ALC / B-155R-PL 26
7.4 B-157 / B-157ALC / B-157R-PL 27
7.5 B-159 / B-159ALC / B-159R-PL 27
7.6 Procedura di assemblaggio 28
7.7 Set di polarizzazione (opzionale) 29
8. Uso del microscopio 30
8.1 Regolazione intensità luminosa 30
8.2 Uso del sistema ALC 30
8.3 Regolazione della frizione 30
8.4 Tavolino 30
8.5 Regolazione distanza interpupillare 31
8.6 Regolazione diottrica 31
8.7 Uso di obiettivi ad immersione 31
8.8 Diaframma di apertura 32
8.9 Uso con batterie ricaricabili 32
8.10 Uso con polarizzatore (opzionale) 32
9. Manutenzione 33
10. Risoluzione dei problemi 34
Smaltimento 35
Sommario