Optika Italy B-1000 Series User manual

Ver. 2.0 2020
INSTRUCTION MANUAL
B-1000 Series
Model
B-1000POL
B-1000POL-I

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-1000POL 4
5.2 B-1000POL-I 6
6. Unpacking 8
7. Assembling 8
7.1 B-1000POL 8
7.2 B-1000POL-I 9
7.3 Microscope assembling 10
7.3.1 B-1000POL 10
7.3.2 B-1000POL-I 12
8. Transmittedlightbrighteldobservationprocedures 16
8.1 B-10000POL 16
8.2 B-1000POL-I 17
9. Useofthemicroscope(transmittedlightbrighteld) 18
9.1 General switch on 18
9.2 Control keyboard 18
9.3 Brightness adjustment 18
9.4 Adjust the observation head 19
9.5 Adjust the interpupillary distance 19
9.6 Diopter adjustment 19
9.7 Use of eye shields 19
9.8 Light path selection 20
9.9 Coarse focus tension adjustment 20
9.10 Focus stop lever 21
9.11 Centering the condenser 21
9.12 E󰀨ectsoftheelddiaphragm 21
9.13 Aperture diaphragm 22
9.14 Stage 22
9.14.1 45° click stop lever 22
10. Polarized transmitted light observation procedures 23
10.1 B-1000POL 23
10.2 B-1000POL-I 24
11. Polarizedreectedlightobservationprocedures 25
11.1 B-1000POL-I 25
12. Use of the microscope in polarized light 26
12.1 Centering of rotatable stage 26
12.2 Nosepiece centering 27
12.3 Checking the extinction of the light 27
12.3.1 B-1000POL 27
12.3.2 B-1000POL-I 28
12.4 Centeringofreectedlightdiaphragms 29
12.4.1Fielddiaphragm(FS) 29
12.4.2 Aperture diaphragm(AS) 30
12.5 Use of tint plates 30
12.6 Use of the Bertrand lens 31
12.7 Useofdi󰀨usinglter(B-1000POL-I) 31
13. Microphotography 32
13.1 Use of C-mount cameras 32
13.2 UseofReexcameras 32
14. Maintenance 33
15. Troubleshooting 34
Equipment disposal 36

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
PHOTO/TV PORT
STAGE ROTATION
LOCK KNOB
NOSEPIECE
DIOPTER
ADJUSTMENT
RING
MAIN SWITCH
EYEPIECE
BERTRAND
LENS
CONDENSER
CENTERING
SCREWS
APERTURE
DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
COARSE UPPER
LIMIT LEVER
OBJECTIVES
STAGE
BERTRAND LENS
CENTERING SCREWS
CONTROL
PANEL
STAGE
CENTERING
SCREWS
5. Overview
5.1 B-1000POL
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

Page 5
COARSE
FOCUSING
KNOB
ANALYZER
FIELD DIAPHRAGM
FINE
FOCUSING
KNOB
X-Y MECHANICAL
STAGE
FILTER
HOLDER
TRANSMITTED LIGHT
POLARIZER
TINT PLATES SLOT
LIGHT PATH
SELECTOR
TENSION
ADJUSTMENT RING
B-1000POL-I(oppositeside)

Page 6
EYEPIECE
ANALYZER
BERTRAND
LENS
APERTURE
DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
OBJECTIVES
STAGE
REFLECTED LIGHT
ILLUMINATOR
BERTRAND LENS
CENTERING SCREWS
DIOPTER
ADJUSTMENT
RING
PHOTO/TV PORT
MAIN SWITCH
CONTROL
PANEL
STAGE
CENTERING
SCREWS
COARSE UPPER
LIMIT LEVER
CONDENSER
CENTERING
SCREWS
TINT PLATES SLOT
NOSEPIECE
FIELD DIAPHRAGM
CENTERING SCREWS
APERTURE DIAPHRAGM
CENTERING SCREWS
5.2 B-1000POL-I
STAGE ROTATION
LOCK KNOB
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

Page 7
FILTER
HOLDER
ANALYZER
FIELD DIAPHRAGM
X-Y MECHANICAL
STAGE
TRANSMITTED LIGHT
POLARIZER
LIGHT PATH
SELECTOR
REFLECTED LIGHT
POLARIZER
DIFFUSING FILTER
SLOT
FIELD DIAPHRAGM
APERTURE DIAPHRAGM
TENSION
ADJUSTMENT RING
COARSE
FOCUSING
KNOB
FINE
FOCUSING
KNOB
TRANSMITTED/
REFLECTED LIGHT
SELECTOR
B-1000POL-I(oppositeside)

Page 8
① Microscope frame
② Observation head
③ Condenser
④ Eyepieces
⑤ Objectives
⑥ Rotatable stage + mechanical stage
⑦ Bertrand lens
⑧ Analyzer
⑨ Tint plates
⑩ Dummy slider
⑪ Nosepiece centering screws
⑫ Allen wrench
⑬ Power supply
⑭ Dust cover
①
②
③
④
⑤
⑥⑦
⑧⑨⑩
⑪
⑫
⑬
⑭
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the microscope box, the microscope parts are the following:
7.1 B-1000POL

Page 9
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Condenser
⑧ Rotatable stage + mechanical stage
⑨ Tint plates
⑩ Diffusing filter
⑪ Reflected light illuminator
⑫ Reflected light polarizer
⑬ Nosepiece centering screws
⑭ Allen wrench
⑮ Power supply
⑯ Dust cover
7.2 B-1000POL-I
⑫
⑬⑭
①
②
③
④
⑤
⑥⑦ ⑧
⑨
⑩
⑪
⑮
⑯

Page 10
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
①
7.3 Microscope assembling
7.3.1 B-1000POL
1. Insert the Bertrand lens ① on the frame and
lock the locking screw ② with the provided Allen
wrench. (Fig. 1)
2. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 2)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
4. Insert the condenser under the stage: position
until it is well inserted into its holder (under the
condenser there is a pin that must fully enter the
holder guide). (Fig. 4)
5. Lock the condenser xing knob ①.

Page 11
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8
③
④
①
②
6. Mount the rotating stage: at the bottom of the
stage there is a spring, push this spring toward
the stage support ①, then push the stage down-
ward ②. (Fig. 5)
7. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 6)
8. Remove the dummy slider from Bertrand lens ③
and insert the analyser ④. (Fig. 7 - 8)

Page 12
FFig. 10ig. 10
FFig. 11ig. 11
FFig. 12ig. 12
①
②
③
④
⑤
FFig. 9ig. 9
9. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 9)
7.3.2 B-1000POL-I
1. Insert the reected light illuminator ① on the
frame and lock the locking screw ② with the
provided Allen wrench. (Fig. 10)
2. Connect the illuminator cable jack to the socket
③ placed in the back side of the frame. (Fig. 11)
3. Insert the Bertrand lens ④ on the reected light
illuminator and lock the locking screw ⑤ with the
provided Allen wrench. (Fig. 12)

Page 13
FFig. 14ig. 14
FFig. 15ig. 15
①
FFig. 16ig. 16
①
②
4. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 13)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
5. Insert both eyepieces into the tubes of the optical
head. (Fig. 14)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
6. Insert the condenser under the stage: position
until it is well inserted into its holder (under the
condenser there is a pin that must fully enter the
holder guide). (Fig. 15)
7. Lock the condenser xing knob ①.
8. Mount the rotating stage: at the bottom of the
stage there is a spring, push this spring toward
the stage support ①, then push the stage down-
ward ②. (Fig. 16)
FFig. 13ig. 13

Page 14
FFig. 19ig. 19
FFig. 20ig. 20
FFig. 18ig. 18
①
②
③
9. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 17)
10. Insert reected light polarizer ①. (Fig. 18)
11. Remove the dummy slider from Bertrand lens ②
and insert the analyser ③. (Fig. 19 - 20)
FFig. 17ig. 17

Page 15
FFig. 21ig. 21
12. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig.
21).

Page 16
Main switch
(Used commands) (Chapter)
Nosepiece
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Aperture diaphragm
Field diaphragm
Nosepiece
Coarse and fine focus knobs
Set the main switch to “I”
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
9)
5
5
5
9
9
9
9
9
9
Coarse and fine focus knobs 5
Brightness adjustment knob 5/9
Bertrand lens
Set the Bertrand lens ring on “O” 12
Remove all filters from the light path Analyzer
Tint plates
12
12
ON-OFF key on the keyboard
Brightness adjustment knob
Turn on the microscope and adjust light intensity 9
5/9
8. Transmittedlightbrighteldobservationprocedures
8.1 B-10000POL

Page 17
(Used commands) (Chapter)
Bertrand lens
Nosepiece
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Turn on the microscope and adjust light intensity
Set the Bertrand lens ring on “O”
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and
focus the specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
12
5
5
5
5
5/9
Remove all filters from the light path
ON-OFF key on the keyboard
Brightness adjustment knob
9
5/9
Analyzer
Tint plates
12
12
Reected light illuminator
Set on “T” the light path selector on the reflected
light illuminator 12
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
9
9
9
9
Aperture diaphragm
Field diaphragm
9
9
Main switch
Set the main switch to “I” 9
8.2 B-1000POL-I

Page 18
③
④
②
FFig. 23ig. 23
FFig. 24ig. 24
⑤
①
FFig. 22ig. 22
①
9. Useofthemicroscope(transmittedlightbrighteld)
9.1 General switch on
To activate the transmitted light illuminator, put the
main switch ①, located on the left side of the stand,
to the position “1”. (Fig. 22)
• For B-1000POL-I model only. There is a three-
position switch on the left side of the stand:
position “I” turns on the transmitted light, position
“II” turns on the reected light and position “O”
turns o󰀨 the microscope.
9.2 Control keyboard
B-1000 illumination can be managed through the
keyboard placed on the left of the stand. (Fig. 23)
• ON-OFF (②): press this key (after switching the
main switch on “1”) to turn ON or OFF the micro-
scope LED.
• BOOST (③): press this button in order to in-
crease the brightness (useful for high-magni-
cation objectives or very opaque specimens).
Do not enable boost mode while observing
with low magnication objectives (4x, 10x)
with fully open diaphragm: the high bright-
ness may hurt user’s eyes.
• AUTO OFF (④): if you want the illuminator to
switch o󰀨 automatically, press this button until 15,
30 or 60 minutes delay is set. After this period of
time, the light will turn o󰀨. You have to press the
ON-OFF button to turn it on again.
9.3 Brightness adjustment
Use the brightness adjustment dial ⑤ on the left side
of the microscope to increase or decrease the light
intensity on the specimen. (Fig. 24)

Page 19
FFig. 25ig. 25
FFig. 26ig. 26
FFig. 28ig. 28
FFig. 27ig. 27
①
③
②
9.4 Adjust the observation head
Loosen the locking screw ①, turn the observation
head to a comfortable position for observation, and
then lock the locking screw again. (Fig. 25)
9.5 Adjust the interpupillary distance
Observing with both eyes, hold the two eyepiece
prism assemblies. Rotate them around their common
axis until the elds of view coincide.
• The graduation on the interpupillary distance
indicator ②, pointed by the spot “.” on the
eyepieceholder,showsthedistancebetween
theoperator’seyes.(Fig.26)
The range of the interpupillary distance is 48-75 mm.
9.6 Diopter adjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ③ to compensate. (Fig. 27)
• The adjustment range is ±5 diopter. The
number indicated on the adjustment ring
graduation should correspond to the
operator’s dioptric correction.
9.7 Use of eye shields
• Use with eyeglasses
Fold rubber eye shields with both hands. Folded eye
shields avoid scratching the lenses of eyeglasses.
(Fig. 28)

Page 20
FFig. 29ig. 29
FFig. 31ig. 31
FFig. 30ig. 30
②
①
• Use without eyeglasses
Raise eye shields and observe at the microscope
placing eyes to the shields, avoiding external light to
disturb the observation. (Fig. 29)
9.8 Light path selection
• The observation head is equipped with an optical
path selector that allows the light to be distributed
to the eyepieces and to the photo / TV port.
1. Move the selector ① to one of the three possible
positions to distribute the light. (Fig. 30)
POSITION LIGHT
IN 100% EYEPIECES
MIDDLE 50% EYEPIECES / 50% TV
OUT 100% TV
9.9 Coarse focus tension adjustment
The tension of the coarse focusing knob is factory
preset.
1. To modify the tension according to personal’s
needs, rotate the ring ② , placed on the right side
of the frame just beside the coarse focus knob.
(Fig. 31)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension .