Optika Italy B-1000 Series User manual

Ver. 2.0 2020
INSTRUCTION MANUAL
B-1000 Series
Model
B-1000POL
B-1000POL-I

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-1000POL 4
5.2 B-1000POL-I 6
6. Unpacking 8
7. Assembling 8
7.1 B-1000POL 8
7.2 B-1000POL-I 9
7.3 Microscope assembling 10
7.3.1 B-1000POL 10
7.3.2 B-1000POL-I 12
8. Transmittedlightbrighteldobservationprocedures 16
8.1 B-10000POL 16
8.2 B-1000POL-I 17
9. Useofthemicroscope(transmittedlightbrighteld) 18
9.1 General switch on 18
9.2 Control keyboard 18
9.3 Brightness adjustment 18
9.4 Adjust the observation head 19
9.5 Adjust the interpupillary distance 19
9.6 Diopter adjustment 19
9.7 Use of eye shields 19
9.8 Light path selection 20
9.9 Coarse focus tension adjustment 20
9.10 Focus stop lever 21
9.11 Centering the condenser 21
9.12 E󰀨ectsoftheelddiaphragm 21
9.13 Aperture diaphragm 22
9.14 Stage 22
9.14.1 45° click stop lever 22
10. Polarized transmitted light observation procedures 23
10.1 B-1000POL 23
10.2 B-1000POL-I 24
11. Polarizedreectedlightobservationprocedures 25
11.1 B-1000POL-I 25
12. Use of the microscope in polarized light 26
12.1 Centering of rotatable stage 26
12.2 Nosepiece centering 27
12.3 Checking the extinction of the light 27
12.3.1 B-1000POL 27
12.3.2 B-1000POL-I 28
12.4 Centeringofreectedlightdiaphragms 29
12.4.1Fielddiaphragm(FS) 29
12.4.2 Aperture diaphragm(AS) 30
12.5 Use of tint plates 30
12.6 Use of the Bertrand lens 31
12.7 Useofdi󰀨usinglter(B-1000POL-I) 31
13. Microphotography 32
13.1 Use of C-mount cameras 32
13.2 UseofReexcameras 32
14. Maintenance 33
15. Troubleshooting 34
Equipment disposal 36

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
PHOTO/TV PORT
STAGE ROTATION
LOCK KNOB
NOSEPIECE
DIOPTER
ADJUSTMENT
RING
MAIN SWITCH
EYEPIECE
BERTRAND
LENS
CONDENSER
CENTERING
SCREWS
APERTURE
DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
COARSE UPPER
LIMIT LEVER
OBJECTIVES
STAGE
BERTRAND LENS
CENTERING SCREWS
CONTROL
PANEL
STAGE
CENTERING
SCREWS
5. Overview
5.1 B-1000POL
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

Page 5
COARSE
FOCUSING
KNOB
ANALYZER
FIELD DIAPHRAGM
FINE
FOCUSING
KNOB
X-Y MECHANICAL
STAGE
FILTER
HOLDER
TRANSMITTED LIGHT
POLARIZER
TINT PLATES SLOT
LIGHT PATH
SELECTOR
TENSION
ADJUSTMENT RING
B-1000POL-I(oppositeside)

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EYEPIECE
ANALYZER
BERTRAND
LENS
APERTURE
DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
OBJECTIVES
STAGE
REFLECTED LIGHT
ILLUMINATOR
BERTRAND LENS
CENTERING SCREWS
DIOPTER
ADJUSTMENT
RING
PHOTO/TV PORT
MAIN SWITCH
CONTROL
PANEL
STAGE
CENTERING
SCREWS
COARSE UPPER
LIMIT LEVER
CONDENSER
CENTERING
SCREWS
TINT PLATES SLOT
NOSEPIECE
FIELD DIAPHRAGM
CENTERING SCREWS
APERTURE DIAPHRAGM
CENTERING SCREWS
5.2 B-1000POL-I
STAGE ROTATION
LOCK KNOB
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

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FILTER
HOLDER
ANALYZER
FIELD DIAPHRAGM
X-Y MECHANICAL
STAGE
TRANSMITTED LIGHT
POLARIZER
LIGHT PATH
SELECTOR
REFLECTED LIGHT
POLARIZER
DIFFUSING FILTER
SLOT
FIELD DIAPHRAGM
APERTURE DIAPHRAGM
TENSION
ADJUSTMENT RING
COARSE
FOCUSING
KNOB
FINE
FOCUSING
KNOB
TRANSMITTED/
REFLECTED LIGHT
SELECTOR
B-1000POL-I(oppositeside)

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① Microscope frame
② Observation head
③ Condenser
④ Eyepieces
⑤ Objectives
⑥ Rotatable stage + mechanical stage
⑦ Bertrand lens
⑧ Analyzer
⑨ Tint plates
⑩ Dummy slider
⑪ Nosepiece centering screws
⑫ Allen wrench
⑬ Power supply
⑭ Dust cover
①
②
③
④
⑤
⑥⑦
⑧⑨⑩
⑪
⑫
⑬
⑭
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the microscope box, the microscope parts are the following:
7.1 B-1000POL

Page 9
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Condenser
⑧ Rotatable stage + mechanical stage
⑨ Tint plates
⑩ Diffusing filter
⑪ Reflected light illuminator
⑫ Reflected light polarizer
⑬ Nosepiece centering screws
⑭ Allen wrench
⑮ Power supply
⑯ Dust cover
7.2 B-1000POL-I
⑫
⑬⑭
①
②
③
④
⑤
⑥⑦ ⑧
⑨
⑩
⑪
⑮
⑯

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FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
①
7.3 Microscope assembling
7.3.1 B-1000POL
1. Insert the Bertrand lens ① on the frame and
lock the locking screw ② with the provided Allen
wrench. (Fig. 1)
2. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 2)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
4. Insert the condenser under the stage: position
until it is well inserted into its holder (under the
condenser there is a pin that must fully enter the
holder guide). (Fig. 4)
5. Lock the condenser xing knob ①.